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Badreddine Douzi

Badreddine Douzi
13
Documents
Identifiants chercheurs

Présentation

- **Career Biography** Once he obtained a diploma in Biotechnology Engineering from University of Sfax in Tunisia, Badreddine Douzi moved to France to start a scientific career in the field of microbiology. He studied biochemistry, structural biology and molecular biology at the University of Aix-Marseille and obtained his Master degree. Then he earned a PhD in biochemistry and structural Biology under the supervision of Dr Romé Voulhoux and Dr Mariella Tegoni. His thesis work was dedicated to the study of molecular mechanisms underlying the assembly and function of a bacterial nanomachine called the type II secretion system (T2SS). Fascinated by these bacterial nanomachines, he was interested during his first postdoctorat, under the supervision of Dr Christian Cambillau, by studying a different secretion system called the type VI secretion system (T6SS) dedicated to the injection of toxins into neighboring cells. Then he moved to the University of British Columbia in the laboratory of Prof Franck Duong to study the molecular mechanisms that coordinates the translocation of colicins across the bacterial envelope. In 2015, he went back to France to join the team of Dr Romé Voulhoux as research associate to work on the T2SS, specifically on the assembly and function of the portal complex called the Secretin. After a short period on the laboratory of Dr Etienne Maisonneuve, Badreddine Douzi was appointed “Chargé de Recherche at the INRA in 2018 in the DynAMic laboratory. - **Research interest** Working on bacterial nanomachines since 2008, Badreddine Douzi main’s interests are to understand, at molecular levels, the mechanisms underlying the traffic of molecules across the bacterial envelope. The transport of molecules is orchestrated by a specialized bacterial apparatus called secretion systems. They are large macromolecular complexes dedicated to the transport of proteins and DNA to the extracellular milieu or directly to host cells. Among the nine secretion systems identified up to date, Badreddine Douzi is currently focused on the study of the assembly and function of the conjugative type IV secretion system (T4SS) in Gram + bacteria. These nanomachines are responsible for the transfer of mobile genetic elements between bacteria and consequently play a crucial role in horizontal gene transfer. He is using complementary approaches (genetics, molecular biology, biochemistry and structural biology) to understand how these nanomachines assembles on the bacterial envelope and ensure the DNA transfer.

Publications

eric-cascales
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Towards a complete structural deciphering of Type VI secretion system

van Son Nguyen , Badreddine Douzi , Eric Durand , Alain Roussel , E. Cascales
Current Opinion in Structural Biology, 2018, 49, pp.77 - 84. ⟨10.1016/j.sbi.2018.01.007⟩
Article dans une revue hal-01780753v1
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Structure–Function Analysis of the C-Terminal Domain of the Type VI Secretion TssB Tail Sheath Subunit

Badreddine Douzi , Laureen Logger , Silvia Spinelli , Stephanie Blangy , Christian Cambillau
Journal of Molecular Biology, 2018, 430 (3), pp.297 - 309. ⟨10.1016/j.jmb.2017.11.015⟩
Article dans une revue hal-01780748v1
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The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly

Melvin G Renault , Jordi Zamarreño Beas , Badreddine Douzi , Maïalène Chabalier , Abdelrahim Zoued
Journal of Molecular Biology, 2018, 430 (18 Pt B), pp.3143-3156. ⟨10.1016/j.jmb.2018.07.018⟩
Article dans une revue hal-01847145v1
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Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli

Badreddine Douzi , Yannick R Brunet , Silvia Spinelli , Valentine Lensi , Stephanie Blangy
Scientific Reports, 2016, 6 (1), ⟨10.1038/srep34405⟩
Article dans une revue hal-01780690v1
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Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17"

Abdelrahim Zoued , Eric Durand , Yannick R Brunet , Silvia Spinelli , Badreddine Douzi
Nature, 2016, 531 (7592), pp.59 - 63. ⟨10.1038/nature17182⟩
Article dans une revue hal-01778575v1
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Structure–Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes

Abdelrahim Zoued , Chloé J Cassaro , Eric Durand , Badreddine Douzi , Alexandre España
Journal of Molecular Biology, 2016, 428 (22), pp.4413 - 4423. ⟨10.1016/j.jmb.2016.08.030⟩
Article dans une revue hal-01780163v1
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The Type VI Secretion TssEFGK-VgrG Phage-Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves As Assembly Platform for Tail Tube/Sheath Polymerization

Yannick R Brunet , Abdelrahim Zoued , Frédéric Boyer , Badreddine Douzi , E. Cascales
PLoS Genetics, 2015, 11 (10), pp.e1005545. ⟨10.1371/journal.pgen.1005545⟩
Article dans une revue hal-01458191v1
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Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody

van Son Nguyen , Laureen Logger , Silvia Spinelli , Aline Desmyter , Thi Thu Hang Le
PLoS ONE, 2015, 10 (e0122187), pp.1-14
Article dans une revue hal-01217205v1
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Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli

Badreddine Douzi , Silvia Spinelli , Stéphanie Blangy , Alain Roussel , Eric Durand
PLoS ONE, 2014, 9 (2), pp.e86918. ⟨10.1371/journal.pone.0086918⟩
Article dans une revue hal-02091448v1

Architecture and assembly of the Type VI secretion system.

Abdelrahim Zoued , Yannick R Brunet , Eric Durand , Marie-Stéphanie Aschtgen , Laureen Logger
Biochim. Biophys. Acta, 2014, 1843 (8), pp.1664--73. ⟨10.1016/j.bbamcr.2014.03.018⟩
Article dans une revue hal-01458220v1
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TssK Is a Trimeric Cytoplasmic Protein Interacting with Components of Both Phage-like and Membrane Anchoring Complexes of the Type VI Secretion System

Abdelrahim Zoued , Eric Durand , Cecilia C. Bebeacua , Yannick R Brunet , Badreddine Douzi
Journal of Biological Chemistry, 2013, 288 (38), pp.27031-27041. ⟨10.1074/jbc.M113.499772⟩
Article dans une revue hal-02091435v1
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Dissection of the TssB-TssC Interface during Type VI Secretion Sheath Complex Formation

Xiang Y. Zhang , Yannick R Brunet , Laureen Logger , Badreddine Douzi , Christian Cambillau
PLoS ONE, 2013, 8 (11), pp.e81074. ⟨10.1371/journal.pone.0081074⟩
Article dans une revue hal-02091436v1
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Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar

Catarina Felisberto-Rodrigues , Eric Durand , Marie-Stéphanie Aschtgen , Stephanie Blangy , Miguel Ortiz-Lombardia
PLoS Pathogens, 2011, 7 (11), pp.e1002386. ⟨10.1371/journal.ppat.1002386⟩
Article dans une revue hal-01458272v1