Cécile Polge

46
Documents

Présentation

Proteolysis plays a key role in controlling muscle growth and atrophy. However, the regulatory mechanisms by which nutrients, hormones and/or physical activity control proteolysis are still poorly understood. The Ubiquitin Proteasome System (UPS) is responsible for the breakdown of the major contractile proteins and is a highly complex and tightly regulated proteolytic system comprising hundreds of enzymes. Understanding the regulatory mechanisms that control muscle mass is compulsory to ultimately preserve muscle strength during aging and many pathological wasting conditions like cancers, infections, trauma, renal failure, etc. Therefore, our final goal is to propose new therapeutic approaches that may prevent or limit muscle loss in catabolic conditions and/or improve muscle recovery following atrophying situations. These studies are of obvious clinical interest for reducing the length of hospitalizations and for improving treatments efficiency, and thus should ultimately result in decreased health care costs.
As a key player in muscle atrophy, the Ubiquitin Proteasome System (UPS) is a potential target for fighting against an exacerbated proteolysis. UPS substrates are labeled by a ubiquitin chain catalyzed by a coordinated enzymatic cascade E1-E2-E3. The polyubiquitinated proteins are then degraded by the 26S proteasome. The UPS regulates numerous cellular processes and the proteasome is common to all the proteins to be degraded. Thus, targeting the proteasome would inhibit the whole UPS-linked cellular protein degradation and would be highly toxic for cells. By contrast, E2-E3 couples (35 and > 600 respectively) are responsible for UPS selectivity, the E3 ligase being responsible for the recognition of the substrate while the E2 defines the type of ubiquitin chain linked to the substrate. Thus, E2-E3 couples represent a more promising target for fighting against muscle atrophy.

Research activities
We demonstrated that α-actin is a UPS substrate in cultured myotubes and in human biopsies. In addition, the muscle-specific E3 ligase MuRF1 targets the myofibrillar actin pool (Polge et al, FASEB J. 2011). We demonstrated that UBE2B, an E2 highly expressed in atrophying skeletal muscle, targets the cytoplasmic pool of actin for MuRF1-independent degradation, which suggests that different E2-E3 couples target distinct pools of actin (Polge et al, JCSM 2016a). We determined that UBE2D2 is not involved in MuRF1-dependent muscle wasting during hindlimb suspension (Polge et al, IJBCB 2016b). Using complementary approaches, we recently identified five E2 enzymes interacting with MuRF1 (E2E1, E2G1, E2J1, E2J2, and E2L3) (Polge et al, JCSM 2018). We showed that telethonin, a newly identified MuRF1 substrate, governed the affinity between MuRF1 and E2E1 or E2J1 (Polge et al, JCSM 2018). We are currently studying the capacity of E2-MuRF1 duos to target the main contractile proteins (actin, myosin heavy chains, …) both in vitro and in vivo (Polge et al, Cells 2018).
We routinely use in vitro approaches and various cellular (C2C12, L6, …), animal (rodents) and human (biopsies) catabolic models. We are specialized in protein-protein interaction studies using complementary approaches like yeast 2- and 3-hybrid and SPR (Biacore).

Publications

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Publications

Structure predictions of MuRF 1‐ UBE2 complexes identify amino acid residues governing interaction selectivity for each MuRF 1‐ E2 pair

Agnès Claustre , Mélodie Malige , Maëlys Macheton , Lydie Combaret , Etienne Lefai et al.
FEBS Journal, 2025, ⟨10.1111/febs.70017⟩
Article dans une revue hal-04993910 v1

Tripartite Split‐GFP for High Throughput Screening of Small Molecules: A Powerful Strategy for Targeting Transient/Labile Interactors like E2‐E3 Ubiquitination Enzymes

Cécile Polge , Stéphanie Cabantous , Daniel Taillandier
ChemBioChem, 2024, 25 (6), ⟨10.1002/cbic.202300723⟩
Article dans une revue hal-04651502 v1
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Knockout of the muscle-specific E3 ligase MuRF1 affects liver lipid metabolism upon dexamethasone treatment in mice

Laurent Mosoni , Arno Germond , Cécile Coudy-Gandilhon , Mélodie Malige , Agnès Claustre et al.
ACS Omega, 2024, 9 (45), pp.45610-45623. ⟨10.1021/acsomega.4c08501&ref=pdf⟩
Article dans une revue hal-04766081 v1
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Induction of ATF4-Regulated Atrogenes Is Uncoupled from Muscle Atrophy during Disuse in Halofuginone-Treated Mice and in Hibernating Brown Bears

Laura Cussonneau , Cécile Coudy-Gandilhon , Christiane Deval , Ghita Chaouki , Mehdi M. Djelloul-Mazouz et al.
International Journal of Molecular Sciences, 2023, 24 (1), pp.621. ⟨10.3390/ijms24010621⟩
Article dans une revue hal-04178468 v1
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A Single Bout of Ultra-Endurance Exercise Reveals Early Signs of Muscle Aging in Master Athletes

Cécile Coudy-Gandilhon , Marine Gueugneau , Christophe Chambon , Daniel Taillandier , Lydie Combaret et al.
International Journal of Molecular Sciences, 2022, 23 (7), pp.3713. ⟨10.3390/ijms23073713⟩
Article dans une revue hal-03776164 v1
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UBE2L3, a Partner of MuRF1/TRIM63, Is Involved in the Degradation of Myofibrillar Actin and Myosin

Dulce Peris-Moreno , Mélodie Malige , Agnès Claustre , Andrea Armani , Cécile Coudy-Gandilhon et al.
Cells, 2021, 10 (8), pp.1974. ⟨10.3390/cells10081974⟩
Article dans une revue hal-03435380 v1
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Muscle Proteomic and Transcriptomic Profiling of Healthy Aging and Metabolic Syndrome in Men

Marine Gueugneau , Cécile Coudy-Gandilhon , Christophe Chambon , Julien Verney , Daniel Taillandier et al.
International Journal of Molecular Sciences, 2021, 22 (8), pp.4205. ⟨10.3390/ijms22084205⟩
Article dans une revue hal-03238377 v1
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Concurrent BMP Signaling Maintenance and TGF-β Signaling Inhibition Is a Hallmark of Natural Resistance to Muscle Atrophy in the Hibernating Bear

Laura Cussonneau , Christian Boyer , Charlotte Brun , Christiane Deval , Emmanuelle Loizon et al.
Cells, 2021, 10 (8), pp.1873. ⟨10.3390/cells10081873⟩
Article dans une revue hal-03314541 v1
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Ubiquitin Ligases at the Heart of Skeletal Muscle Atrophy Control

Dulce Peris-Moreno , Laura Cussonneau , Lydie Combaret , Cécile Polge , Daniel Taillandier
Molecules, 2021, 26 (2), pp.407. ⟨10.3390/molecules26020407⟩
Article dans une revue (article de synthèse) hal-03182261 v1
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Mitophagy and mitochondria biogenesis are differentially induced in rat skeletal muscles during immobilization and/or remobilization

Christiane Deval , Julie Calonne , Cécile Coudy-Gandilhon , Emilie Vazeille , Daniel D. Bechet et al.
International Journal of Molecular Sciences, 2020, 21 (10), pp.3691. ⟨10.3390/ijms21103691⟩
Article dans une revue hal-02935975 v2
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MuRF1/TRIM63, Master regulator of muscle mass

Dulce Peris-Moreno , Daniel Taillandier , Cécile Polge
International Journal of Molecular Sciences, 2020, 21 (18), pp.6663. ⟨10.3390/ijms21186663⟩
Article dans une revue hal-02952557 v1

Skeletal muscle atrogenes: from rodent models to human pathologies

Daniel Taillandier , Cécile Polge
Article dans une revue (article de synthèse) hal-02192519 v1
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Muscle wasting in patients with end‐stage renal disease or early‐stage lung cancer: common mechanisms at work

Julien Aniort , Alexandre Stella , Carole Philipponnet , Anaïs Poyet , Cécile Polge et al.
Journal of Cachexia, Sarcopenia and Muscle, 2019, 10 (2), pp.323-337. ⟨10.1002/jcsm.12376⟩
Article dans une revue hal-02167448 v1

Magnesium transport and homeostasis-related gene expression in skeletal muscle of young and old adults: analysis of the transcriptomic data from the PROOF cohort Study.

Cécile Coudy-Gandilhon , Marine Gueugneau , Daniel Taillandier , Lydie Combaret , Cécile Polge et al.
Magnesium Research, 2019, 32 (3), pp.72-82. ⟨10.1684/mrh.2019.0458⟩
Article dans une revue hal-02520159 v1
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UBE2E1 Is Preferentially Expressed in the Cytoplasm of Slow-Twitch Fibers and Protects Skeletal Muscles from Exacerbated Atrophy upon Dexamethasone Treatment (vol 7, 214, 2018)

Cécile Polge , Julien Aniort , Andrea Armani , Agnes Claustre , Cécile Coudy-Gandilhon et al.
Cells, 2018, 7 (12), ⟨10.3390/cells7120242⟩
Article dans une revue hal-02622197 v1
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UBE2E1 Is Preferentially Expressed in the Cytoplasm of Slow-Twitch Fibers and Protects Skeletal Muscles from Exacerbated Atrophy upon Dexamethasone Treatment

Cécile Polge , Julien Aniort , Andrea Armani , Agnes Claustre , Cécile Coudy-Gandilhon et al.
Cells, 2018, 7 (11), ⟨10.3390/cells7110214⟩
Article dans une revue hal-01958290 v1
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A muscle-specific MuRF1-E2 network requires stabilization of MuRF1-E2 complexes by telethonin, a newly identified substrate

Cécile Polge , Stéphanie Cabantous , Christiane Deval , Agnes Claustre , Antoine Hauvette et al.
Journal of Cachexia, Sarcopenia and Muscle, 2018, 1 (1), pp.129-145. ⟨10.1002/jcsm.12249⟩
Article dans une revue hal-01730002 v1

UBE2D2 is not involved in MuRF1-dependent muscle wasting during hindlimb suspension

Cécile Polge , Nathalie Koulmann , Agnes Claustre , Marianne Jarzaguet , Bernard Serrurier et al.
International Journal of Biochemistry and Cell Biology, 2016, 79, pp.488-493. ⟨10.1016/j.biocel.2016.06.019⟩
Article dans une revue hal-01512230 v1
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Docosahexaenoic acid-supplementation prior to fasting prevents muscle atrophy in mice

Christiane Deval , Frédéric Capel , Brigitte Laillet , Cécile Polge , Daniel Bechet et al.
Journal of Cachexia, Sarcopenia and Muscle, 2016, 7 (5), pp.587-603. ⟨10.1002/jcsm.12103⟩
Article dans une revue hal-01512083 v1

Upregulation of MuRF1 and MAFbx participates to muscle wasting upon gentamicin-induced acute kidney injury

Julien Aniort , Cécile Polge , Agnes Claustre , Lydie Combaret , Daniel Bechet et al.
International Journal of Biochemistry and Cell Biology, 2016, 79, pp.505-516. ⟨10.1016/j.biocel.2016.04.006⟩
Article dans une revue hal-01512019 v1
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UBE2B is implicated in myofibrillar protein loss in catabolic C2C12 myotubes

Cécile Polge , Roza Leulmi , Marianne Jarzaguet , Agnes Claustre , Lydie Combaret et al.
Journal of Cachexia, Sarcopenia and Muscle, 2016, 7 (3), pp.377-87. ⟨10.1002/jcsm.12060⟩
Article dans une revue hal-02633728 v1

Lower skeletal muscle capillarization in hypertensive elderly men

Marine Gueugneau , Cécile Coudy-Gandilhon , Bruno Meunier , Lydie Combaret , Daniel Taillandier et al.
Experimental Gerontology, 2016, 76, pp.80-88. ⟨10.1016/j.exger.2016.01.013⟩
Article dans une revue hal-01297805 v1
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The delayed recovery of the remobilized rat tibialis anterior muscle reflects a defect in proliferative and terminal differentiation that impairs early regenerative processes

Lamia Slimani , Emilie Vazeille , Christiane Deval , Bruno Meunier , Cécile Polge et al.
Journal of Cachexia, Sarcopenia and Muscle, 2015, 6 (1), pp.73-83. ⟨10.1002/jcsm.12011⟩
Article dans une revue hal-01901485 v1
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Role of E2-Ub-conjugating enzymes during skeletal muscle atrophy

Cécile Polge , Didier Attaix , Daniel Taillandier
Frontiers in Physiology, 2015, 6, n.p. ⟨10.3389/fphys.2015.00059⟩
Article dans une revue (article de synthèse) hal-02639857 v1

Skeletal muscle lipid content and oxidative activity in relation to muscle fiber type in aging and metabolic syndrome

Marine Gueugneau , Cécile Coudy-Gandilhon , Laetitia Theron , Bruno Meunier , Christiane Barboiron et al.
Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 2015, 70 (5), pp.566-576. ⟨10.1093/gerona/glu086⟩
Article dans une revue hal-01812812 v1

Proteomics of muscle chronological ageing in post-menopausal women

Marine Gueugneau , Cécile Coudy-Gandilhon , Ophélie Gourbeyre , Christophe Chambon , Lydie Combaret et al.
BMC Genomics, 2014, 15 (1), pp.1165. ⟨10.1186/1471-2164-15-1165⟩
Article dans une revue hal-04217877 v1
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Apoptosis in capillary endothelial cells in ageing skeletal muscle

Huijuan Wang , Anne Listrat , Bruno Meunier , Marine Gueugneau , Cécile C. Coudy-Gandilhon et al.
Aging Cell, 2014, 13 (2), pp.254-262. ⟨10.1111/acel.12169⟩
Article dans une revue hal-02636978 v1
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Proteomics of muscle chronological ageing in post-menopausal women

Marine Gueugneau , Cecile Coudy-Gandilhon , Ophelie Gourbeyre , Christophe Chambon , Lydie Combaret et al.
BMC Genomics, 2014, 15 (1165), pp.1165. ⟨10.1186/1471-2164-15-1165⟩
Article dans une revue hal-01543975 v1
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Glutathione S-Transferases Interact with AMP-Activated Protein Kinase: Evidence for S-Glutathionylation and Activation In Vitro

Anna Klaus , Sarah Zorman , Alexandre Berthier , Cécile Polge , Sacnicte Ramirez et al.
PLoS ONE, 2013, 8 (5), pp.e62497. ⟨10.1371/journal.pone.0062497⟩
Article dans une revue hal-01987684 v1

Deciphering the ubiquitin proteome: Limits and advantages of high throughput global affinity purification-mass spectrometry approaches

Cécile Polge , Sandrine Uttenweiler-Joseph , Roza Leulmi , Anne-Elisabeth Heng , Odile Burlet-Schiltz et al.
International Journal of Biochemistry and Cell Biology, 2013, 45 (10), pp.2136-2146. ⟨10.1016/j.biocel.2013.05.031⟩
Article dans une revue istex hal-02502779 v1

The worsening of tibialis anterior muscle atrophy during recovery post-immobilization correlates with enhanced connective tissue area, proteolysis, and apoptosis

Lamia Slimani , Didier D. Micol , Julien Amat , Geoffrey Delcros , Bruno Meunier et al.
AJP - Endocrinology and Metabolism, 2012, 303 (11), pp.E1335 - E1347. ⟨10.1152/ajpendo.00379.2012⟩
Article dans une revue hal-02651117 v1
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A two-dimensional screen for AMPK substrates identifies tumor suppressor fumarate hydratase as a preferential AMPKα2 substrate.

Anna Klaus , Cécile Polge , Sarah Zorman , Yolanda Auchli , René Brunisholz et al.
Journal of Proteomics, 2012, 75 (11), pp.3304-13. ⟨10.1016/j.jprot.2012.03.040⟩
Article dans une revue inserm-00696309 v1

Muscle actin is polyubiquitinylated in vitro and in vivo and targeted for breakdown by the E3 ligase MuRF1

Cécile Polge , Anne-Elisabeth Heng , Marianne Jarzaguet , Sophie Ventadour , Agnes Claustre et al.
FASEB Journal, 2011, 25 (11), pp.3790-3802. ⟨10.1096/fj.11-180968⟩
Article dans une revue hal-02420104 v1
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Voies protéolytiques du muscle : oeuvres solistes ou ensemble orchestré ?

Lydie Combaret , Daniel Béchet , Daniel Taillandier , Cécile Polge , Didier Attaix
Les Cahiers de Myologie, 2011, 4, pp.10-12
Article dans une revue hal-03318545 v1
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Evidence for the Existence in Arabidopsis thaliana of the Proteasome Proteolytic Pathway: ACTIVATION IN RESPONSE TO CADMIUM.

Cécile Polge , Michel Jaquinod , Frances Holzer , Jacques Bourguignon , Linda Walling et al.
Journal of Biological Chemistry, 2009, 284 (51), pp.35412-24. ⟨10.1074/jbc.M109.035394⟩
Article dans une revue hal-00483927 v1

Mitochondrial kinases and their molecular interaction with cardiolipin.

Uwe Schlattner , Malgorzata Tokarska-Schlattner , Sacnicte Ramirez , Anna Brückner , Laurence Kay et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 2009, 1788 (10), pp.2032-47. ⟨10.1016/j.bbamem.2009.04.018⟩
Article dans une revue inserm-00422473 v1
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Yeast two-hybrid, a powerful tool for systems biology.

Anna Brückner , Cécile Polge , Nicolas Lentze , Daniel Auerbach , Uwe Schlattner
International Journal of Molecular Sciences, 2009, 10 (6), pp.2763-88. ⟨10.3390/ijms10062763⟩
Article dans une revue inserm-00422470 v1

Homo-oligomerization and activation of AMP-activated protein kinase are mediated by the kinase domain alphaG-helix.

Roland Scholz , Marianne Suter , Théodore Weimann , Cécile Polge , Petr V. Konarev et al.
Journal of Biological Chemistry, 2009, 284 (40), pp.27425-37. ⟨10.1074/jbc.M109.047670⟩
Article dans une revue inserm-00422467 v1

β -Subunits of the SnRK1 Complexes Share a Common Ancestral Function Together with Expression and Function Specificities; Physical Interaction with Nitrate Reductase Specifically Occurs via AKIN β 1-Subunit

Cécile Polge , Mathieu Jossier , Pierre Crozet , Lionel Gissot , Martine Thomas
Plant Physiology, 2008, 148 (3), pp.1570-1582. ⟨10.1104/pp.108.123026⟩
Article dans une revue hal-03314679 v1
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Modifications in endopeptidase and 20S proteasome expression and activities in cadmium treated tomato (Solanum lycopersicum L.) plants

Wahbi Djebali , Philippe Gallusci , Cécile Polge , Latifa Boulila , Nathalie Galtier et al.
Planta, 2008, 227 (3), pp.625 - 639. ⟨10.1007/s00425-007-0644-6⟩
Article dans une revue istex hal-00188302 v1

SNF1/AMPK/SnRK1 kinases, global regulators at the heart of energy control?

Cécile Polge , Martine Thomas
Trends in Plant Science, 2007, 12 (1), pp.20-8. ⟨10.1016/j.tplants.2006.11.005⟩
Article dans une revue istex hal-00154622 v1

AKINbetagamma contributes to SnRK1 heterotrimeric complexes and interacts with two proteins implicated in plant pathogen resistance through its KIS/GBD sequence

Lionel L. Gissot , Cécile Polge , Mathieu Jossier , Thomas Girin , Jean-Pierre Bouly et al.
Plant Physiology, 2006, 142 (3), pp.931-944. ⟨10.1104/pp.106.087718⟩
Article dans une revue hal-02668884 v1

AKINbeta3, a plant specific SnRK1 protein, is lacking domains present in yeast and mammals non-catalytic ?-subunits

Cécile Polge , Lionel Gissot , Jean-Pierre Bouly , Thomas Lemaitre , Martin Kreis et al.
Plant Molecular Biology, 2004, 56 (5), pp.747-759. ⟨10.1007/s11103-004-5111-1⟩
Article dans une revue istex hal-03314592 v1