Clément Nizak

Présentation

My current research focuses on enzymes and antibodies as experimental models to tackle fundamental questions related to bio-molecule function and evolution. Our experiments are based on droplet microfluidics, phage display and high-throughput DNA sequencing technologies. Our approaches are strongly motivated by computational analysis and theory.

I work at ESPCI Paris in collaboration with Andrew Griffiths (Laboratory of Biochemistry) and at CIRB-College de France in collaboration with Olivier Rivoire (Statistical Biology).

I teach part of the Systems Biology course at ESPCI and the ICFP Masters program of ENS Paris.

Publications

Article dans une revue (28)

Continuous emergence of phototaxis in Dictyostelium discoideum Damien Genettais,Charles Bernard,Félix Geoffroy,Clément Nizak,Sandrine Adiba PLoS ONE, 2025, 20 (5), pp.e0321614. ⟨10.1371/journal.pone.0321614⟩ Article dans une revue hal-05343361v1
Inference and design of antibody specificity: From experiments to models and back Jorge Fernandez-De-Cossio-Diaz,Guido Uguzzoni,Kévin Ricard,Francesca Anselmi,Clément Nizaket al. PLoS Computational Biology, 2024, 20 (10), pp.e1012522. ⟨10.1371/journal.pcbi.1012522⟩ Article dans une revue hal-04778023v2
Parameters and determinants of responses to selection in antibody libraries Steven Schulz,Sébastien Boyer,Matteo Smerlak,Simona Cocco,Rémi Monassonet al. PLoS Computational Biology, 2021, 17 (3), pp.e1008751. ⟨10.1371/journal.pcbi.1008751⟩ Article dans une revue hal-03190048v2
High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics Annabelle Gérard,Adam Woolfe,Guillaume Mottet,Marcel Reichen,Carlos Castrillónet al. Nature Biotechnology, 2020, 38, pp.715-721. ⟨10.1038/s41587-020-0466-7⟩ Article dans une revue pasteur-02505776v1
Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring Klaus Eyer,Raphaël C L Doineau,Carlos E Castrillon,Luis Briseño-Roa,Vera Menrathet al. Nature Biotechnology, 2017, 35 (10), pp.977 - 982. ⟨10.1038/nbt.3964⟩ Article dans une revue hal-01639647v1
Synthesis of new hydrophilic rhodamine based enzymatic substrates compatible with droplet-based microfluidic assays Johan Fenneteau,Dany Chauvin,Andrew D. Griffiths,Clément Nizak,Janine Cossy Chemical Communications, 2017, 53 (39), pp.5437-5440. ⟨10.1039/c7cc01506b⟩ Article dans une revue hal-02104894v1
Hierarchy and extremes in selections from pools of randomized proteins Sébastien Boyer,Dipanwita Biswas,Ananda Kumar Soshee,Natale Scaramozzino,Clément Nizaket al. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (13), pp.3482-3487. ⟨10.1073/pnas.1517813113⟩ Article dans une revue hal-04128959v1
Hierarchy and extremes in selections from pools of randomized proteins Sébastien Boyer,Dipanwita Biswas,Anandakumar Soshee,Natate Scaramozzino,Clément Nizaket al. Proceedings of the National Academy of Sciences of the United States of America, 2016, ⟨10.1073/pnas.1517813113⟩ Article dans une revue hal-01301847v1
Recombinant antibody mediated delivery of organelle-specific DNA pH sensors along endocytic pathways Souvik Modi,S. Haider,Clément Nizak,Yamuna Krishnan Nanoscale, 2014, 6 (2), pp.1144-52. ⟨10.1039/c3nr03769j⟩ Article dans une revue hal-00959788v1
An evolutionarily significant unicellular strategy in response to starvation stress in Dictyostelium social amoebae Darja Dubravcic,Minus van Baalen,Clément Nizak F1000Research, 2014, 3, pp.133. ⟨10.12688/f1000research.4218.1⟩ Article dans une revue hal-01064077v1
Selection of Arginine-Rich Anti-Gold Antibodies Engineered for Plasmonic Colloid Self-Assembly Purvi Jain,Anandakumar Soshee,S.Shankara Narayanan,Jadab Sharma,Christian Girardet al. Journal of Physical Chemistry C, 2014, 118 (26), 10.1021/jp502118n. ⟨10.1021/jp502118n⟩ Article dans une revue hal-00983923v3
General in vitro method to analyze the interactions of synthetic polymers with human antibody repertoires Anandakumar Soshee,Stefan Zürcher,N. Spencer,A. Halperin,Clément Nizak Biomacromolecules, 2014, 15 (1), pp.113-21. ⟨10.1021/bm401360y⟩ Article dans une revue hal-00959789v1
An evolutionarily significant unicellular strategy in response to starvation in social amoebae Dictyostelium social amoebae [version 2; referees: 2 approved] Darja Dubravcic,Minus van Baalen,Clément Nizak F1000Research, 2014, 3, pp.133. ⟨10.12688/f1000research.4218.2⟩ Article dans une revue hal-02392322v1
Two DNA nanomachines map pH changes along intersecting endocytic pathways inside the same cell. Souvik Modi,Clément Nizak,Sunaina Surana,Saheli Halder,Yamuna Krishnan Nature Nanotechnology, 2013, 8 (6), pp.459-67. ⟨10.1038/nnano.2013.92⟩ Article dans une revue hal-00832208v1
Selection and use of intracellular antibodies (intrabodies). Sandrine Moutel,Clément Nizak,Franck Perez Methods in Molecular Biology, 2012, 907, pp.667-79. ⟨10.1007/978-1-61779-974-7_37⟩ Article dans une revue hal-00728051v1
Laser microstructuration of three-dimensional enzyme reactors in microfluidic channels Monica Iosin,Teodora Scheul,Clément Nizak,Olivier Stephan,Simion Astileanet al. Microfluidics and Nanofluidics, 2011, 10 (3), pp.685-690. ⟨10.1007/s10404-010-0698-9⟩ Article dans une revue istex hal-00728055v1
From Grazing Resistance to Pathogenesis: The Coincidental Evolution of Virulence Factors Sandrine Adiba,Clément Nizak,Minus van Baalen,Erick Denamur,Frantz Depaulis PLoS ONE, 2010, 5 (8), pp.e11882. ⟨10.1371/journal.pone.0011882⟩ Article dans une revue hal-00528524v1
Characterization of single chain antibody targets through yeast two hybrid. Ole Vielemeyer,Clément Nizak,Ana Joaquina Jimenez,Arnaud Echard,Bruno Goudet al. BMC Biotechnology, 2010, 10 (1), pp.59. ⟨10.1186/1472-6750-10-59⟩ Article dans une revue inserm-00617227v1
A multi-Fc-species system for recombinant antibody production S. Moutel,A. El Marjou,O. Vielemeyer,Clément Nizak,P. Benarochet al. BMC Biotechnology, 2009, pp.PMID: 19245715 Article dans une revue hal-00565195v1
Direct selection of monoclonal phosphospecific antibodies without prior phosphoamino acid mapping O. Vielemeyer,H. Yuan,S. Moutel,R. Saint-Fort,D. Tanget al. Journal of Biological Chemistry, 2009, pp.PMID: 19473967 Article dans une revue hal-00565193v1
Exploitation of other social amoebae by Dictyostelium caveatum Clément Nizak,Rj Fitzhenry,Rh Kessin PLoS ONE, 2007, pp.PMID: 17299592 Article dans une revue hal-00565197v1
Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery R. Sannerud,M. Marie,Clément Nizak,Ha Dale,Karin Pernet-Gallayet al. Molecular Biology of the Cell, 2006, pp.PMID: 16421253 Article dans une revue hal-00565238v1
Rab6A and Rab6A' GTPases play non-overlapping roles in membrane trafficking E. del Nery,S. Miserey-Lenkei,T. Falguières,Clément Nizak,Ludger Johanneset al. Traffic, 2006, pp.PMID: 16536738 Article dans une revue hal-00565201v1
Selection and application of recombinant antibodies as sensors of rab protein conformation Clément Nizak,S. Moutel,Bruno Goud,Franck Perez Methods in Enzymology, 2005, pp.PMID: 16473583 Article dans une revue hal-00565236v1
Golgi inheritance under a block of anterograde and retrograde traffic Clément Nizak,R. Sougrat,F. Jollivet,A. Rambourg,Bruno Goudet al. Traffic, 2004, pp.PMID: 15030570 Article dans une revue hal-00565237v1
Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Clément Nizak,S. Martin-Lluesma,S. Moutel,A. Roux,Te Kreiset al. Traffic, 2003, pp.PMID: 14617357 Article dans une revue hal-00565239v1
Recombinant antibodies to the small GTPase Rab6 as conformation sensors Clément Nizak,Solange Monier,Elaine del Nery,Sandrine Moutel,Bruno Goudet al. Science, 2003, 300 (5621), pp.PMID: 12738866. ⟨10.1126/science.1083911⟩ Article dans une revue hal-00565240v1
CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family Franck Perez,Karin Pernet-Gallay,Clément Nizak,Hv Goodson,Te Kreiset al. Journal of Cell Biology, 2002, pp.PMID: 11854307 Article dans une revue hal-00565241v1

Chapitre d'ouvrage (1)

Selection and Use of Intracellular Antibodies Sandrine Moutel,Clément Nizak,Franck Perez Methods in molecular biology, pp.491-503, 2018 Chapitre d'ouvrage hal-02104898v1

Pré-publication, Document de travail (1)

Continuous emergence of phototaxis in Dictyostelium discoideum Damien Genettais,Charles Bernard,Felix Geoffroy,Clément Nizak,Sandrine Adiba 2024 Pré-publication, Document de travail hal-04673041v1

Thèse (1)

Développement d'outils pour l'étude de la dynamique de l'appareil de Golgi en interphase et en mitose Clément Nizak Biophysique [physics.bio-ph]. Université Paris-Diderot - Paris VII, 2003. Français. ⟨NNT : ⟩ Thèse tel-00190898v1