Didier Flament

Publications

Article dans une revue (42)

On the origins and variation of nucleotide skews of archaeal genomes Adrien Paravel,Clémence Mottez,Romain Puech,Didier Flament,Hubert F Beckeret al. Frontiers in Microbiology, 2026, 17, pp.1727296. ⟨10.3389/fmicb.2026.1727296⟩ Article dans une revue hal-05543510v1
Wide diversity and complex evolution of M42 aminopeptidases with contrasted functional properties in Archaea Emilie Chagny,Najwa Taib,Daphna Fenel,Eric Girard,Simonetta Gribaldoet al. Molecular Biology and Evolution, 2025, 42 (10), ⟨10.1093/molbev/msaf233⟩ Article dans une revue hal-05274166v1
Evolutionary and functional insights into the Ski2-like helicase family in Archaea: a comparison of Thermococcales ASH-Ski2 and Hel308 activities Manon Batista,Petra Langendijk-Genevaux,Marta Kwapisz,Isabelle Canal,Duy khanh Phunget al. NAR Genomics and Bioinformatics, 2024, 6 (1), lqae026 (16p.). ⟨10.1093/nargab/lqae026⟩ Article dans une revue hal-04573206v1
Cooperation between two modes for DNA replication initiation in the archaeon &lt;i&gt;Thermococcus barophilus&lt;/i&gt; Logan Mc Teer,Yann Moalic,Valérie Cueff-Gauchard,Ryan Catchpole,Gaëlle Hogrelet al. mBio, 2024, 15, ⟨10.1128/mbio.03200-23⟩ Article dans une revue hal-04546373v1
Cooperation between two modes for DNA replication initiation in the archaeon Thermococcus barophilus Logan Mc Teer,Yann Moalic,Valérie Cueff-Gauchard,Ryan Catchpole,Gaëlle Hogrelet al. mBio, 2024, 15 (4), ⟨10.1128/mbio.03200-23⟩ Article dans une revue hal-04728152v1
DNA-binding mechanism and evolution of replication protein A Clément Madru,Markel Martínez-Carranza,Sébastien Laurent,Alessandra Alberti,Maelenn Chevreuilet al. Nature Communications, 2023, 14 (1), pp.2326. ⟨10.1038/s41467-023-38048-w⟩ Article dans une revue hal-04112308v1
DNA polymerization in icy moon abyssal pressure conditions Lorenzo Carré,Ghislaine Henneke,Etienne Henry,Didier Flament,Éric Girardet al. Astrobiology, 2023, pp.0201. ⟨10.1089/ast.2021.0201⟩ Article dans une revue hal-03944643v1
Structural and functional determinants of the archaeal 8-oxoguanine-DNA glycosylase AGOG for DNA damage recognition and processing Coste Franck,Stéphane Goffinont,Julien Cros,Virginie Gaudon,Guérin Martineet al. Nucleic Acids Research, 2022, ⟨10.1093/nar/gkac932⟩ Article dans une revue hal-03841526v1
Characterization of a small tRNA ‐binding protein that interacts with the archaeal proteasome complex Gaëlle Hogrel,Laura Marino-Puertas,Sébastien Laurent,Ziad Ibrahim,Jacques Covèset al. Molecular Microbiology, 2022, Bacterial macromolecular machineries, 118 (1-2), pp.16-29. ⟨10.1111/mmi.14948⟩ Article dans une revue hal-03701716v1
Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase Mirna Hajj,Petra Langendijk-Genevaux,Manon Batista,Yves Quentin,Sébastien Laurentet al. Biomolecules, 2021, 11 (7), pp.950. ⟨10.3390/biom11070950⟩ Article dans une revue hal-03365138v1
RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery Duy Khanh Phung,Clarisse Etienne,Manon Batista,Petra Langendijk-Genevaux,Yann Moalicet al. Nucleic Acids Research, 2020, 48 (7), pp.3832-3847. ⟨10.1093/nar/gkaa052⟩ Article dans une revue hal-03028340v1
Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea Gaelle Hogrel,Yang Lu,Nicolas Alexandre,Audrey Bossé,Remi Dulermoet al. Biomolecules, 2020, 10 (7), 1045 (17p.). ⟨10.3390/biom10071045⟩ Article dans une revue hal-04202612v1
Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus Gaëlle Hogrel,Yang Lu,Sébastien Laurent,Etienne Henry,Clarisse Etienneet al. Nucleic Acids Research, 2018, 46 (11), pp.5651-5663. ⟨10.1093/nar/gky322⟩ Article dans une revue hal-02390072v1
Development of an Effective 6-Methylpurine Counterselection Marker for Genetic Manipulation in Thermococcus barophilus Tiphaine Birien,Axel Thiel,Ghislaine Henneke,Didier Flament,Yann Moalicet al. Genes, 2018, 9 (2), pp.77. ⟨10.3390/genes9020077⟩ Article dans une revue hal-04202052v1
Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of eIF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive Sébastien Laurent,Adrien Richard,Odile Mulner-Lorillon,Julia Morales,Didier Flamentet al. Frontiers in Genetics, 2014, 5, pp.117. ⟨10.3389/fgene.2014.00117⟩ Article dans une revue hal-01079758v1
Genetic manipulations of the hyperthermophilic piezophilic archaeon Thermococcus barophilus. Axel Thiel,Grégoire Michoud,Yann Moalic,Didier Flament,Mohamed Jebbar Applied and Environmental Microbiology, 2014, 80 (7), pp.2299-306. ⟨10.1128/AEM.00084-14⟩ Article dans une revue hal-01019712v1
An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs Pierre-François Pluchon,Thomas Fouqueau,Christophe Crezé,Sébastien Laurent,Julien Briffotauxet al. PLoS ONE, 2013, 8 (11), pp.1-15. ⟨10.1371/journal.pone.0079707⟩ Article dans une revue hal-00911795v1
Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels. Christophe Creze,Alessio Ligabue,Sébastien Laurent,Roxane Lestini,Sergey P. Laptenoket al. Journal of Biological Chemistry, 2012, 287 (19), pp.15648-60. ⟨10.1074/jbc.M112.346361⟩ Article dans une revue hal-00808519v1
Structure and function of a novel endonuclease acting on branched DNA substrates C. Creze,Roxane Lestini,Joëlle Kühn,Alessio Ligabue,Hubert F. Beckeret al. Biochemical Society Transactions, 2011, 39 (1), pp.145-149. ⟨10.1042/BST0390145⟩ Article dans une revue hal-00838893v1
MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms. Agnès Groisillier,Cécile Hervé,Alexandra Jeudy,Etienne Rebuffet,Pierre François Pluchonet al. Microb Cell Fact, 2010, 9, pp.45. ⟨10.1186/1475-2859-9-45⟩ Article dans une revue hal-00609166v1
The glycine-rich motif of Pyrococcus abyssi DNA polymerase D is critical for protein stability. Benoît Castrec,Sébastien Laurent,Ghislaine Henneke,Didier Flament,Jean-Paul Raffin Journal of Molecular Biology, 2010, 396 (4), pp.840-848. ⟨10.1016/j.jmb.2010.01.006⟩ Article dans une revue hal-00609913v1
Binding to PCNA in Euryarchaeal DNA Replication requires two PIP motifs for DNA polymerase D and one PIP motif for DNA polymerase B Benoît Castrec,Christophe Rouillon,Ghislaine Henneke,Didier Flament,Joël Querellouet al. Journal of Molecular Biology, 2009, 394 (2), pp.209-218. ⟨10.1016/j.jmb.2009.09.044⟩ Article dans une revue hal-00609928v1
Structure and function of a novel endonuclease acting on branched DNA substrates. Bin Ren,Joelle Kühn,Laurence Meslet-Cladiere,Julien Briffotaux,Cedric Noraiset al. EMBO Journal, 2009, 28 (16), pp.2479-89. ⟨10.1038/emboj.2009.192⟩ Article dans une revue hal-00406013v1
The heterodimeric primase from the euryarchaeon Pyrococcus abyssi: a multifunctional enzyme for initiation and repair? Magali Le Breton,Ghislaine Henneke,Cédric Norais,Didier Flament,Hannu Myllykallioet al. Journal of Molecular Biology, 2007, 374 (5), pp.1172-85. ⟨10.1016/j.jmb.2007.10.015⟩ Article dans une revue hal-00195212v1
Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families Didier Flament,Tristan Barbeyron,Murielle Jam,Philippe Potin,Mirjam Czjzeket al. Applied and Environmental Microbiology, 2007, 73 (14), pp.4691-4694. ⟨10.1128/AEM.00496-07⟩ Article dans une revue hal-00616725v1
Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi Claire Geslin,Mélusine Gaillard,Didier Flament,K. Rouault,Marc Le Romanceret al. Journal of Bacteriology, 2007, 189 (12), pp.4510-4519. ⟨10.1128/JB.01896-06⟩ Article dans une revue hal-00616744v1
A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen. Laurence Meslet-Cladiére,Cédric Norais,Joëlle Kuhn,Julien Briffotaux,Jerry W Sloostraet al. Journal of Molecular Biology, 2007, 372 (5), pp.1137-48. ⟨10.1016/j.jmb.2007.06.056⟩ Article dans une revue hal-00193755v1
Structural insights into a new homodimeric self-activated GTPase family. Stéphanie Gras,Valérie Chaumont,Bernard Fernandez,Philippe Carpentier,Fabienne Charrier-Savourninet al. EMBO Reports, 2007, 8 (6), pp.569-75. ⟨10.1038/sj.embor.7400958⟩ Article dans une revue hal-00195309v1
DNA polymerase switching on homotrimeric PCNA at the replication fork of the euryarchaea Pyrococcus abyssi Christophe Rouillon,Ghislaine Henneke,Didier Flament,Joël Querellou,Jean-Paul Raffin Journal of Molecular Biology, 2007, 369 (2), pp.343-355. ⟨10.1016/j.jmb.2007.03.054⟩ Article dans une revue hal-00617495v1
The Hyperthermophilic Euryarchaeota Pyrococcus abyssi Likely Requires the Two DNA Polymerases D and B for DNA Replication Ghislaine Henneke,Didier Flament,Ulrich Hübscher,Joël Querellou,Jean-Paul Raffin Journal of Molecular Biology, 2005, 350 (1), pp.53-64. ⟨10.1016/j.jmb.2005.04.042⟩ Article dans une revue hal-05586459v1
The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours. M. Jam,D. Flament,J. Allouch,P. Potin,Dolorès Thionet al. Biochemical Journal, 2005, 385, pp.703-713 Article dans une revue hal-00092292v1
The endo-β-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans : two paralogue enzymes with different molecular organizations and catalytic behaviours Murielle Jam,Didier Flament,Julie Allouch,Philippe Potin,Laurent Thionet al. Biochemical Journal, 2005, 385 (3), pp.703-713. ⟨10.1042/BJ20041044⟩ Article dans une revue hal-05586491v1
Genetic elements of Thermococcales D. Prieur,G. Erauso,C. Geslin,S. Lucas,M. Gaillardet al. Biochemical Society Transactions, 2004, 32 (2), pp.184-187. ⟨10.1042/bst0320184⟩ Article dans une revue hal-05587290v1
Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas Yannick Gueguen,Jean-Paul Cadoret,Didier Flament,Catherine Barreau-Roumiguière,Anne-Laure Girardotet al. Gene, 2003, 303, pp.139-145. ⟨10.1016/S0378-1119(02)01149-6⟩ Article dans une revue istex hal-02498520v1
An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi Georges Cohen,Valérie Barbe,Didier Flament,Michael Galperin,Roland Heiliget al. Molecular Microbiology, 2003, 47 (6), pp.1495-1512. ⟨10.1046/j.1365-2958.2003.03381.x⟩ Article dans une revue hal-04141223v1
Comment on &quot;The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1&quot;: evidence that the unique N-terminal sequence proposed comes from a maltodextrin phosphorylase Jean-Luc Rolland,Yannick Gueguen,Didier Flament,Yann Pouliquen,Peter Streetet al. Extremophiles, 2002, 6 (4), pp.349-350. ⟨10.1007/s00792-001-0258-z⟩ Article dans une revue istex hal-05587322v1
Replication Factor C from the Hyperthermophilic Archaeon Pyrococcus abyssi Does Not Need ATP Hydrolysis for Clamp-loading and Contains a Functionally Conserved RFC PCNA-binding Domain Ghislaine Henneke,Yannick Gueguen,Didier Flament,Philippe Azam,Joël Querellouet al. Journal of Molecular Biology, 2002, 323 (5), pp.795-810. ⟨10.1016/s0022-2836(02)01028-8⟩ Article dans une revue hal-05587314v1
Characterization of the maltooligosyl trehalose synthase from the thermophilic archaeon Sulfolobus acidocaldarius Yannick Gueguen,Jean-Luc Rolland,Silke Schroeck,Didier Flament,Sophie Defretinet al. FEMS Microbiology Letters, 2001, 194 (2), pp.201-206. ⟨10.1111/j.1574-6968.2001.tb09470.x⟩ Article dans une revue hal-05587331v1
Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon &lt;i&gt;Pyrococcus abyssi&lt;/i&gt;: DNA polymerases from &lt;i&gt;Pyrococcus abyssi&lt;/i&gt; Yannick Gueguen,Jean-Luc Rolland,Odile Lecompte,Philippe Azam,Gisèle Le Romanceret al. European Journal of Biochemistry, 2001, 268 (22), pp.5961-5969. ⟨10.1046/j.0014-2956.2001.02550.x⟩ Article dans une revue hal-04094338v1
Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi Joseph Boudrant,Jean Luc Rolland,Didier Flament,Yannick Gueguen,Jacques Dietrich Applied and Environmental Microbiology, 2001, 67 (10), pp.4504-11. ⟨10.1128/AEM.67.10.4504-4511.2001⟩ Article dans une revue hal-03059758v1
Expression, purification, crystallization and preliminary X-ray analysis of the ι-carrageenase from Alteromonas fortis Gurvan Michel,Didier Flament,Tristan Barbeyron,Thierry Vernet,Bernard Kloareget al. Acta Crystallographica Section D: Biological Crystallography, 2000, 56 (6), pp.766-768. ⟨10.1107/S0907444900004844⟩ Article dans une revue istex hal-05587498v1
Expression, purification, crystallization and preliminary X-ray analysis of the κ-carrageenase from Pseudoalteromonas carrageenovora Gurvan Michel,Tristan Barbeyron,Didier Flament,Thierry Vernet,Bernard Kloareget al. Acta Crystallographica Section D: Biological Crystallography, 1999, 55 (4), pp.918-920. ⟨10.1107/s0907444998018526⟩ Article dans une revue istex hal-05587507v1

Pré-publication, Document de travail (1)

Structuring effects of archaeal replication origins Clémence Mottez,Romain Puech,Didier Flament,Hannu Myllykallio 2023 Pré-publication, Document de travail hal-04311015v1

Communication dans un congrès (1)

Real-Time Fluorescence Assays to Monitor DNA Polymerase Activities Etienne Henry,Ghislaine Henneke,Didier Flament 61st Biophysical Society Annual Meeting, Feb 2017, New Orleans LA USA, United States. pp.513a, ⟨10.1016/j.bpj.2016.11.2776⟩ Communication dans un congrès hal-04461169v1

Chapitre d'ouvrage (1)

Deep-sea Thermococcales and their Genetic Elements: Plasmids and Viruses Daniel Prieur,Gaël Erauso,Didier Flament,Mélusine Gaillard,Claire Geslinet al. Extremophiles, Elsevier, pp.253-272, 2006, Methods in Microbiology ; 35, ⟨10.1016/S0580-9517(08)70014-X⟩ Chapitre d'ouvrage hal-00620699v1