Emmanuel Margeat

Publications

Article dans une revue (40)

Triple Labeling Resolves a GPCR Intermediate State by Using Three-Color Single Molecule FRET Léo Bonhomme,Ecenaz Bilgen,Caroline Clerté,Jean-Philippe Pin,Philippe Rondardet al. Journal of the American Chemical Society, 2025, 147 (21), pp.17689-17700. ⟨10.1021/jacs.4c18364⟩ Article dans une revue hal-05079126v1
Single-stranded DNA drives σ subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations Rishi Kishore Vishwakarma,Nils Marechal,Zakia Morichaud,Mickaël Blaise,Emmanuel Margeatet al. Nucleic Acids Research, 2025, 53 (7), pp.gkaf272. ⟨10.1093/nar/gkaf272⟩ Article dans une revue hal-05037511v1
Structural basis of orientated asymmetry in a mGlu heterodimer Weizhu Huang,Nan Jin,Jia Guo,Cangsong Shen,Chanjuan Xuet al. Nature Communications, 2024, 15 (1), pp.10345. ⟨10.1038/s41467-024-54744-7⟩ Article dans une revue hal-04819462v1
Concerted conformational changes control metabotropic glutamate receptor activity Nathalie Lecat-Guillet,Robert Quast,Hongkang Liu,Emmanuel Bourrier,Thor Mølleret al. Science Advances , 2023, 9 (22), pp.eadf1378. ⟨10.1126/sciadv.adf1378⟩ Article dans une revue hal-04121751v1
Dissecting metabotropic glutamate receptor activation by single molecule FRET using minimally invasive labeling strategies Leo Bonhomme,Robert Quast,Nathalie Lecat-Guillet,Jean-Philippe Pin,Philippe Rondardet al. Biophysical Journal, 2023, 122 (3), pp.58a. ⟨10.1016/j.bpj.2022.11.521⟩ Article dans une revue hal-04598517v1
Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins Ganesh Agam,Christian Gebhardt,Milana Popara,Rebecca Mächtel,Julian Folzet al. Nature Methods, 2023, 20 (4), pp.523 - 535. ⟨10.1038/s41592-023-01807-0⟩ Article dans une revue hal-04120543v1
A modular spring-loaded actuator for mechanical activation of membrane proteins A. Mills,N. Aissaoui,Damien Maurel,J. Elezgaray,F. Morvanet al. Nature Communications, 2022, 13 (1), pp.3182. ⟨10.1038/s41467-022-30745-2⟩ Article dans une revue hal-03751001v1
Cryo-EM structure of transcription termination factor Rho from Mycobacterium tuberculosis reveals bicyclomycin resistance mechanism Emmanuel Saridakis,Rishi Vishwakarma,Josephine Lai-Kee-Him,Kevin Martin,Isabelle Simonet al. Communications Biology, 2022, 5 (1), pp.120. ⟨10.1038/s42003-022-03069-6⟩ Article dans une revue hal-03563179v1
Viro-fluidics: Real-time analysis of virus production kinetics at the singlecell level Joëlle Eid,Marius Socol,Antoine Naillon,Jérôme Feuillard,Luca Ciandriniet al. Biophysical Reports, 2022, 2 (3), pp.100068. ⟨10.1016/j.bpr.2022.100068⟩ Article dans une revue hal-03751935v2
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices Eitan Lerner,Anders Barth,Jelle Hendrix,Benjamin Ambrose,Victoria Birkedalet al. eLife, 2021, 10, ⟨10.7554/elife.60416⟩ Article dans une revue hal-03191929v1
Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state Anne-Marinette Cao,Robert B Quast,Fataneh Fatemi,Philippe Rondard,Jean-Philippe Pinet al. Nature Communications, 2021, 12 (1), pp.5426. ⟨10.1038/s41467-021-25620-5⟩ Article dans une revue hal-03352901v2
Single-molecule FRET on its way to structural biology in live cells Robert Quast,Emmanuel Margeat Nature Methods, 2021, 18 (4), pp.344-345. ⟨10.1038/s41592-021-01084-9⟩ Article dans une revue hal-03191934v1
Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies Thales Fernandes,Oscar Saavedra,Emmanuel Margeat,Pierre-Emmanuel Milhiet,Luca Costa Scientific Reports, 2020, 10 (1), pp.7098. ⟨10.1038/s41598-020-62529-3⟩ Article dans une revue hal-02568884v1
Studying GPCR conformational dynamics by single molecule fluorescence Robert Quast,Emmanuel Margeat Molecular and Cellular Endocrinology, 2019, 493, pp.110469. ⟨10.1016/j.mce.2019.110469⟩ Article dans une revue hal-02346229v1
A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription Cédric Nadiras,Mildred Delaleau,Annie Schwartz,Emmanuel Margeat,Marc Boudvillain Biochemistry, 2019, 58 (7), pp.865-874. ⟨10.1021/acs.biochem.8b01216⟩ Article dans une revue hal-02067416v1
Accurate Determination of Human CPR Conformational Equilibrium by smFRET Using Dual Orthogonal Noncanonical Amino Acid Labeling Robert Quast,Fataneh Fatemi,Michel Kranendonk,Emmanuel Margeat,Gilles Truan ChemBioChem, 2019, 20 (5), pp.659-666. ⟨10.1002/cbic.201800607⟩ Article dans une revue hal-02174030v1
Chloride ions stabilize the glutamate-induced active state of the metabotropic glutamate receptor 3 Amélie S. Tora,Xavier Rovira,Anne-Marinette Cao,Alexandre Cabaye,Linnea Olofssonet al. Neuropharmacology, 2018, 140, pp.275-286. ⟨10.1016/j.neuropharm.2018.08.011⟩ Article dans une revue hal-02346230v1
Single-molecule analysis reveals the mechanism of transcription activation in M. tuberculosis Rishi Kishore Vishwakarma,Anne-Marinette Cao,Zakia Morichaud,Ayyappasamy Sudalaiyadum Perumal,Emmanuel Margeatet al. Science Advances , 2018, 4 (5), pp.eaao5498. ⟨10.1126/sciadv.aao5498⟩ Article dans une revue hal-02346231v1
Oligomerization of a G protein-coupled receptor in neurons controlled by its structural dynamics Thor C Møller,Jérôme Hottin,Caroline Clerté,Jurriaan M. Zwier,Thierry Durrouxet al. Scientific Reports, 2018, 8 (1), ⟨10.1038/s41598-018-28682-6⟩ Article dans une revue hal-02398134v1
Competitive folding of RNA structures at a termination-antitermination site Soraya Ait-Bara,Caroline Clerté,Nathalie Declerck,Emmanuel Margeat RNA, 2017, 23 (5), pp.721-734. ⟨10.1261/rna.060178.116⟩ Article dans une revue hal-01602716v1
Optimisation of vectorisation property: A comparative study for a secondary amphipathic peptide Karidia Konate,Mattias F. Lindberg,Anaïs Vaissiere,Carole Jourdan,Gudrun Aldrianet al. International Journal of Pharmaceutics, 2016, 509 (1-2), pp.71--84. ⟨10.1016/j.ijpharm.2016.05.030⟩ Article dans une revue hal-01878021v1
Imaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies Mireia Ferrer,Caroline Clerté,Célia Chamontin,Eugenia Basyuk,Sébastien Lainéet al. Nucleic Acids Research, 2016, 44 (16), pp.7922-7934. ⟨10.1093/nar/gkw511⟩ Article dans une revue hal-02141204v1
Automatic detection of diffusion modes within biological membranes using back-propagation neural network Patrice Dosset,Patrice Rassam,Laurent Fernandez,Cedric Espenel,Eric Rubinsteinet al. BMC Bioinformatics, 2016, 17 (1), pp.197. ⟨10.1186/s12859-016-1064-z⟩ Article dans une revue inserm-01322561v1
Plasmonic Nanoantennas Enable Forbidden Förster Dipole–Dipole Energy Transfer and Enhance the FRET Efficiency Juan de Torres,Mathieu Mivelle,Satish Babu Moparthi,Hervé Rigneault,Niek F. Van Hulstet al. Nano Letters, 2016, 16 (10), pp.6222-6230. ⟨10.1021/acs.nanolett.6b02470⟩ Article dans une revue hal-01421637v1
Direct observation of the translocation mechanism of transcription termination factor Rho V. Gocheva,Antoine Le Gall,M. Boudvillain,E. Margeat,Marcelo Nollmann Nucleic Acids Research, 2015, 43 (4), pp.2367-2377. ⟨10.1093/nar/gkv085⟩ Article dans une revue hal-01171254v1
Fine tuning of sub-millisecond conformational dynamics controls metabotropic glutamate receptors agonist efficacy Linnea Olofsson,S. Felekyan,Etienne Doumazane,Pauline Scholler,Ludovic Fabreet al. Nature Communications, 2014, 5, pp.5206. ⟨10.1038/ncomms6206⟩ Article dans une revue hal-01943332v1
Chromatin insulator factors involved in long-range DNA interactions and their role in the folding of the Drosophila genome Jutta Vogelmann,Antoine Le Gall,Stephanie Déjardin,Frédéric Allemand,Adrien Gamotet al. PLoS Genetics, 2014, 10 (8), ⟨10.1371/journal.pgen.1004544⟩ Article dans une revue hal-02640560v1
The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP). Emilie Soares,Annie Schwartz,Marcelo Nollmann,Emmanuel Margeat,Marc Boudvillain Nucleic Acids Research, 2014, 42 (14), pp.9270-9284. ⟨10.1093/nar/gku595⟩ Article dans une revue hal-01180888v1
Recruitment, Assembly, and Molecular Architecture of the SpoIIIE DNA Pump Revealed by Superresolution Microscopy Jean-Bernard Fiche,Diego Cattoni,Nele Diekmann,Julio Mateos Langerak,Caroline Clerteet al. PLoS Biology, 2013, 11 (5), pp.e1001557. ⟨10.1371/journal.pbio.1001557⟩ Article dans une revue hal-03273727v1
Analysis of helicase-RNA interactions using nucleotide analog interference mapping. Annie Schwartz,Makhlouf Rabhi,Emmanuel Margeat,Marc Boudvillain Methods in Enzymology, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩ Article dans une revue hal-00726256v1
Mutagenesis-Based Evidence for an Asymmetric Configuration of the Ring-Shaped Transcription Termination Factor Rho Makhlouf Rabhi,Veronika Gocheva,Frederique Jacquinot,Andrea Lee,Emmanuel Margeatet al. Journal of Molecular Biology, 2011, 405 (2), pp.497-518. ⟨10.1016/j.jmb.2010.11.006⟩ Article dans une revue istex hal-00608241v1
Structural Insight into the Mycobacterium tuberculosis Rv0020c Protein and Its Interaction with the PknB Kinase Christian Roumestand,Jade Leiba,Nathalie Galophe,Emmanuel Margeat,André Padillaet al. Structure, 2011, 19 (10), pp.1525-1534. ⟨10.1016/j.str.2011.07.011⟩ Article dans une revue hal-02282715v1
Structural Insight into the Mycobacterium tuberculosis Rv0020c Protein and Its Interaction with the PknB Kinase Christian Roumestand,Jade Leiba,Nathalie Galophe,Emmanuel Margeat,André Padillaet al. Structure, 2011, 19 (10), pp.1525-1534. ⟨10.1016/j.str.2011.07.011⟩ Article dans une revue hal-03726704v1
Keeping up to speed with the transcription termination factor Rho motor. Marc Boudvillain,Marcelo Nollmann,Emmanuel Margeat Transcription, 2010, 1 (2), pp.70-75. ⟨10.4161/trns.1.2.12232⟩ Article dans une revue hal-00591655v1
A stepwise 2 '-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase Annie Schwartz,Makhlouf Rabhi,Frederique Jacquinot,Emmanuel Margeat,A. Rachid Rahmouniet al. Nature Structural and Molecular Biology, 2009, 16 (12), pp.1309-1316. ⟨10.1038/nsmb.1711⟩ Article dans une revue istex hal-00525325v1
Quantitative analysis of the binding of ezrin to large unilamellar vesicles containing phosphatidylinositol 4,5 bisphosphate. Guillaume Blin,Emmanuel Margeat,Kévin Carvalho,Catherine A. Royer,Christian Royet al. Biophysical Journal, 2008, 94 (3), pp.1021-33. ⟨10.1529/biophysj.107.110213⟩ Article dans une revue inserm-00193998v1
Inducer-modulated cooperative binding of the tetrameric CggR repressor to operator DNA. Silvia Zorrilla,Thierry Doan,Carlos Alfonso,Emmanuel Margeat,Alvaro Ortegaet al. Biophysical Journal, 2007, 92 (9), pp.3215-27. ⟨10.1529/biophysj.106.095109⟩ Article dans une revue hal-00164236v1
Fructose-1,6-bisphosphate acts both as an inducer and as a structural cofactor of the central glycolytic genes repressor (CggR). Silvia Zorrilla,Denis Chaix,Alvaro Ortega,Carlos Alfonso,Thierry Doanet al. Biochemistry, 2007, 46 (51), pp.14996-5008. ⟨10.1021/bi701805e⟩ Article dans une revue istex hal-00315549v1
Transcription termination factor Rho can displace streptavidin from biotinylated RNA Annie Schwartz,Emmanuel Margeat,A. Rachid Rahmouni,Marc Boudvillain Journal of Biological Chemistry, 2007, 282 (43), pp.31469-31476. ⟨10.1074/jbc.M706935200⟩ Article dans une revue hal-00176284v1
The H-NS dimerization domain defines a new fold contributing to DNA recognition. Vanessa Bloch,Yinshan Yang,Emmanuel Margeat,Alain Chavanieu,Marie Thérèse Augéet al. Nature Structural Biology, 2003, 10 (3), pp.212-8. ⟨10.1038/nsb904⟩ Article dans une revue istex hal-00257410v1

Communication dans un congrès (3)

Structural basis for resistance of Mycobacterium tuberculosis Rho to bicyclomycin Emmanuel Saridakis,Rishi Vishwakarma,Joséphine Lai Kee Him,Kevin Martin,Isabelle Simonet al. Helicases & Nucleic Acid-Based Machines: Structure, Mechanism & Regulation, and Roles in HumanDisease, Jul 2021, Paris, France Communication dans un congrès hal-03419029v1
4D vertical imaging of plant growth in physiological conditions with two photons microscopy Houssam Hajjoul,Marc Lartaud,Christophe Perin,Philippe Nacry,Soazig Guyomarc'Het al. Hot Topics in Molecular Imaging-TOPIM, Jan 2014, Les Houches, France Communication dans un congrès hal-01594253v1
Second Harmonic Generation in GaN-based photonic crystals for single molecule investigations Dominique Coquillat,Jeremie Torres,Marine Le Vassor d'Yerville,David Cassagne,Frederic Teppeet al. GALLIUM NITRIDE MATERIALS AND DEVICES VII, Jan 2012, San Francisco (CA), United States. pp.82621R, ⟨10.1117/12.905593⟩ Communication dans un congrès hal-00704334v1

Poster de conférence (5)

Viro-fluidics: a new strategy to study HIV release in real time at the single cell and single particle scales J. Eid,M. Socol,Antoine Naillon,J. Feuillard,Emmanuel Margeatet al. XXIIIes Journées francophones de virologie, Apr 2021, En ligne, France Poster de conférence hal-03424136v1
Structural Dynamics of Single Metabotropic Glutamate Receptor Dimers Robert B Quast,Anne-Marinette Cao,Fataneh Fatemi,Linnea Olofsson,Philippe Rondardet al. Biophysical Society. Biophysical Society 64th Annual Meeting, Feb 2020, San Diego (CA), United States. Elsevier, BIOPHYSICAL JOURNAL, 118 (3), pp.58a, 2020, ⟨10.1016/j.bpj.2019.11.493⟩ Poster de conférence hal-04772779v1
La viro-fluidique une nouvelle stratégie pour étudier le relargage du VIH-1 Joëlle Eid,Marius Socol,Benoît Charlot,Emmanuel Margeat,Marylène Mougel Les journées ANRS: "Recherche dur le VIH/SIDA et les hépatites virales: quels nouveaux enjeux?", Nov 2019, Paris, France Poster de conférence hal-02327002v1
Single-molecule analysis of E. coli Rho translocase, Helicase, transcription termination activities, and its interactions with RNA polymerase R. Vishwakarma,A. Nord,F. Pedaci,M. Boudvillain,E. Margeat Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France Poster de conférence hal-02902115v1
Relationships between Rho and RNA polymerase in living bacteria C. Clerte,O. Manier,J-B. Fiche,M. Boudvillain,L. Bossiet al. Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France Poster de conférence hal-02902111v1

Autre publication scientifique (1)

The FRET-based structural dynamics challenge -- community contributions to consistent and open science practices Eitan Lerner,Benjamin Ambrose,Anders Barth,Victoria Birkedal,Scott C. Blanchardet al. 2020 Autre publication scientifique hal-03002379v1

Pré-publication, Document de travail (2)

Conformational Changes of the ABC Transporter BmrA Depend on Membrane Curvature Alicia Damm,Kemil Belhadji,Raj Kumar Sadhu,Su-Jin Paik,Aurélie Di-Ciccoet al. 2025 Pré-publication, Document de travail hal-05343468v1
Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state Anne-Marinette Cao,Robert Quast,Fataneh Fatemi,Philippe Rondard,Jean-Philippe Pinet al. 2021 Pré-publication, Document de travail hal-03191940v1