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Fernanda FONSECA

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#### **EDUCATION** **Habilitation à Diriger des Recherches (HDR).** Université de Technologie de Compiègne, 2010. Title : « Stabilization of bio functionalities trough an approach combining the understanding of mechanisms and the product-process engineering” **Ph.D. Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech),**2001**. Ph.D. thesis title: "Influence of cryoprotection and freezing on the acidification activity of thermopile lactic acid bacteria: relationships between biological and thermodynamic properties." **Master Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech), **1997**. M.Sc. thesis title: "Study of the freezing step on the activity of frozen lactic acid starters." Engineer** in **Chemical Engineering**, Montevideo University (Uruguay), **1996.** **SCIENTIFIC EXPERIENCE** ------------------------- **Research Director (since January 2015),** in Paris-Saclay Food and Bioproduct Engineering research unit (UMR 782 SayFood INRAE-AgroParisTech), Versailles-Grignon Centre, at the French National Institute for Agriculture, Food and Environment (INRAE), France. **Researcher Scientist** (**September 2001 – December 2014**), in the Joint Research Unit for Food Process Engineering and Microbiology (UMR 782 GMPA INRA-AgroParisTech), Versailles-Grignon Centre, Institut National de la Recherche Agronomique (INRA), France. **Main fields of expertise:**Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. - Bioproducts: micro-organisms, proteins, food products. - Stabilization processes: freezing and freeze-drying - Biophysical characterization of bioproducts (thermal analysis, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy) **Research approach:** Focusing on biophysical and chemical behaviors of bioproducts during stabilization processes and by relating them to biological and functional responses, to identify and quantify the mechanisms governing the degradation and preservation of bioproducts during the stabilization processes. The ambition is also to find general principles for simplifying and rationalizing the development of newly formulated products and the stabilization process optimization, with particular attention to limiting the environmental impact.

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Publications

frederic-jamme

FTIR micro-spectroscopy using synchrotron-based and thermal source-based radiation for probing live bacteria

Julie Meneghel , Stéphanie Passot , Frédéric Jamme , Stéphane Lefrançois , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2020, 412 (26), pp.7049-7061. ⟨10.1007/s00216-020-02835-x⟩
Article dans une revue hal-03004447v1

Subcellular membrane fluidity of Lactobacillus delbrueckii subsp. bulgaricus under cold and osmotic stress

Julie Meneghel , Stéphanie Passot , Stéphanie Cenard , Matthieu Refregiers , Frédéric Jamme
Applied Microbiology and Biotechnology, 2017, 101 (18), pp.6907 - 6917. ⟨10.1007/s00253-017-8444-9⟩
Article dans une revue hal-01634459v1

Understanding the cryotolerance of lactic acid bacteria using combined synchrotron infrared and fluorescence microscopies

Stéphanie Passot , Julie Gautier , Frédéric Jamme , Stéphanie Cenard , Paul Dumas
Analyst, 2015, 140 (17), pp.5920-5928. ⟨10.1039/C5AN00654F⟩
Article dans une revue hal-01536496v1
Image document

Physiological and biochemical responses of Yarrowia lipolytica to dehydration induced by air-drying and freezing

Caroline Pénicaud , Sophie Landaud-Liautaud , Frederic Jamme , Pauline Talbot , Marielle Bouix
PLoS ONE, 2014, 9 (10), ⟨10.1371/journal.pone.0111138⟩
Article dans une revue hal-01195500v1

Synchrotron UV fluorescence microscopy for determining membrane fluidity modification of single bacteria with temperatures

Stéphanie S. Passot , Frederic Jamme , M. Refregiers , Julie J. Gautier , Stéphanie Cenard
Biomedical Spectroscopy and Imaging, 2014, 3 (3), pp.203-210. ⟨10.3233/BSI-140062⟩
Article dans une revue hal-02629450v1
Image document

Structural study of lipid droplet using synchrotron label-free multimodal imaging

Frederic Jamme , Isabelle Bouchez , Caroline Pénicaud , Stéphanie Passot , Fernanda Fonseca
4. International Conference on Physics and Biological Systems, Oct 2018, Gif-sur-Yvette, France. , 2018, 4th International Conference on Physics and Biological Systems 2018
Poster de conférence hal-02733903v1

Synchrotron label free multimodal imaging to explore lipid droplets in living cells

Frederic Jamme , Isabelle Bouchez , Caroline Pénicaud , Stéphanie Passot , Fernanda Fonseca
Conférence Jacques Monod Analyses moléculaires de l’organisation et du remodelage des membranes, Apr 2017, Roscoff, France. , 2017
Poster de conférence hal-02736520v1

Mapping membrane fluidity by fluorescence anisotropy to better understand bacterial toloerance to osmotic and cold stresses encountered during freezing

Julie Meneghel , Stéphanie Passot , Stéphanie Cenard , Jiawei Xu , Matthieu Refregiers
52. Annual Meeting of the Society for Cryobiology, Jul 2015, Ostrava, Czech Republic, Czech Republic. Elsevier Ltd, Cryobiology, 71, pp.549, 2015, Cryobiology. ⟨10.1016/j.cryobiol.2015.10.052⟩
Poster de conférence hal-01536575v1

Towards the measurement of membrane fluidity at subzero temperatures for improving cell cryopreservation

Stéphanie Passot , Frédéric Jamme , Matthieu Refregiers , J. Gautier , Stéphanie Cenard
European Conference on the Spectroscopy of Biological Molecules (ECSBM 15), Aug 2013, Oxford, United Kingdom. Biomedical Spectroscopy and Imaging, 3(3), 2013, Biomedical Spectroscopy and Imaging
Poster de conférence hal-01628117v1