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Fernanda FONSECA

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#### **EDUCATION** **Habilitation à Diriger des Recherches (HDR).** Université de Technologie de Compiègne, 2010. Title : « Stabilization of bio functionalities trough an approach combining the understanding of mechanisms and the product-process engineering” **Ph.D. Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech),**2001**. Ph.D. thesis title: "Influence of cryoprotection and freezing on the acidification activity of thermopile lactic acid bacteria: relationships between biological and thermodynamic properties." **Master Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech), **1997**. M.Sc. thesis title: "Study of the freezing step on the activity of frozen lactic acid starters." Engineer** in **Chemical Engineering**, Montevideo University (Uruguay), **1996.** **SCIENTIFIC EXPERIENCE** ------------------------- **Research Director (since January 2015),** in Paris-Saclay Food and Bioproduct Engineering research unit (UMR 782 SayFood INRAE-AgroParisTech), Versailles-Grignon Centre, at the French National Institute for Agriculture, Food and Environment (INRAE), France. **Researcher Scientist** (**September 2001 – December 2014**), in the Joint Research Unit for Food Process Engineering and Microbiology (UMR 782 GMPA INRA-AgroParisTech), Versailles-Grignon Centre, Institut National de la Recherche Agronomique (INRA), France. **Main fields of expertise:**Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. - Bioproducts: micro-organisms, proteins, food products. - Stabilization processes: freezing and freeze-drying - Biophysical characterization of bioproducts (thermal analysis, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy) **Research approach:** Focusing on biophysical and chemical behaviors of bioproducts during stabilization processes and by relating them to biological and functional responses, to identify and quantify the mechanisms governing the degradation and preservation of bioproducts during the stabilization processes. The ambition is also to find general principles for simplifying and rationalizing the development of newly formulated products and the stabilization process optimization, with particular attention to limiting the environmental impact.

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Publications

712996
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Membrane lipid composition of Carnobacterium maltaromaticum CNCM I-3298, a highly cryoresistant lactic bacterium

Ha Phuong Ta , C. Clarisse , E. Maes , N. Yamakawa , Y. Guérardel
Chemistry and Physics of Lipids, 2023, 255, pp.105326. ⟨10.1016/j.chemphyslip.2023.105326⟩
Article dans une revue hal-04397076v1
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Multi-objective optimization of frozen and freeze-dried Lactobacillus delbrueckii subsp. bulgaricus CFL1 production via the modification of fermentation conditions

Maria de Lourdes Tovilla Coutino , Stephanie Passot , Ioan-Cristian Trelea , Marie-Hélène Ropers , Yann Gohon
Journal of Applied Microbiology, 2023, 134 (2), pp.1-34. ⟨10.1093/jambio/lxad003⟩
Article dans une revue hal-04345553v1
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Insights into the mechanisms of L. salivarius CECT5713 resistance to freeze-dried storage

Maria Guerrero Sanchez , Stéphanie Passot , Sarrah Ghorbal , Sonia Campoy , Monica Olivares
Cryobiology, 2023, 112, pp.104556. ⟨10.1016/j.cryobiol.2023.104556⟩
Article dans une revue hal-04395152v1
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Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

M. Guerrero Sanchez , Stephanie Passot , S. Campoy , M. Olivares , F. Fonseca
Applied Microbiology and Biotechnology, 2022, 106 (1), pp.57-80. ⟨10.1007/s00253-021-11694-0⟩
Article dans une revue hal-03822336v1
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Insights into lactic acid bacteria cryoresistance using FTIR micro-spectroscopy

Amélie Girardeau , Stephanie Passot , Julie Meneghel , Stéphanie Cenard , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2022, 414 (3), pp.1425-1443. ⟨10.1007/s00216-021-03774-x⟩
Article dans une revue hal-03552585v1
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Effect of protective agents on the storage stability of freeze‑dried Ligilactobacillus salivarius CECT5713

Maria Guerrero Sanchez , Stéphanie Passot , Sonia Campoy , Monica Olivares , Fernanda Fonseca
Applied Microbiology and Biotechnology, 2022, 106, pp.7235-7249. ⟨10.1007/s00253-022-12201-9⟩
Article dans une revue hal-03845922v1
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The transfer temperature from slow cooling to cryogenic storage is critical for optimal recovery of cryopreserved mammalian cells

Peter Kilbride , Julie Meneghel , Fernanda Fonseca , John Morris
PLoS ONE, 2021, 16 (11), pp.e0259571. ⟨10.1371/journal.pone.0259571⟩
Article dans une revue hal-03446319v1
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Physical events occurring during the cryopreservation of immortalized human T cells

Julie Meneghel , Peter Kilbride , John G. Morris , Fernanda Fonseca
PLoS ONE, 2019, 14 (5), pp.e0217304. ⟨10.1371/journal.pone.0217304⟩
Article dans une revue hal-02154629v1
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Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Fernanda Fonseca , Caroline Pénicaud , E. Elizabeth Tymczyszyn , Andrea Gómez-Zavaglia , Stephanie Passot
Applied Microbiology and Biotechnology, 2019, 103 (17), pp.6867-6883. ⟨10.1007/s00253-019-10002-1⟩
Article dans une revue hal-03824544v1
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Influence of culture conditions on the technological properties of Carnobacterium maltaromaticum CNCM I-3298 starters

Amélie Girardeau , C. Puentes , S. Keravec , P. Peteuil , Ioan-Cristian Trelea
Journal of Applied Microbiology, 2019, 126 (5), pp.1468-1479. ⟨10.1111/jam.14223⟩
Article dans une revue hal-02154448v1

Differential scanning calorimetry applied to the preservation of bacteria and mammalian cells

Fernanda Fonseca , Stéphanie Passot
JCAT53 - 53èmes Journées de Calorimétrie et d'Analyse Thermique, May 2023, Palaiseau, France
Communication dans un congrès hal-04395225v1

Environmental performances of the production of dried lactic acid bacteria concentrates protected by fructo-oligosaccharides ICEF14, June 20 th to 23 rd 2023, Nantes, France

Stéphanie Passot , Maite Gagneten , Camille Quentier , Stéphanie Cenard , Baptiste Gauducheau-Chevalier
International Congress on Engineering and Food, Jun 2023, Nantes, France
Communication dans un congrès hal-04397246v1

Stabilization of Lactiplantibacillus plantarum WCFS1 during freezing, freeze-drying, and storage using legume wastewater extracts containing galacto-oligosaccharides

Gonçalo N. Martins , Maria Guerrero Sanchez , Angela D. Carboni , Fernanda Fonseca , Sonia Campoy
10th CQM Annual Meeting, Jun 2023, Funchal, Portugal
Communication dans un congrès hal-04397545v1

A critical transfer temperature from slow cooling to cryogenic storage for optimal recovery across a range of cryopreserved mammalian cells.

Julie Meneghel , Peter Kilbride , Fernanda Fonseca , John Morris
Annual Meeting of the Society for Cryobiology, Jul 2022, Dublin, Ireland
Communication dans un congrès hal-04397788v1

Bacterial cell adaptation to environmental stress during fermentation improves cryo-and drying resistance: A focus on membrane properties

Maria de L Tovilla-Coutino , Stéphanie Passot , Jérôme Delettre , Sarrah Ghorbal , Marie-Hélène Ropers
5th Microbial Stress Conference of the European Federation of Biotechnology, Nov 2020, Virtual, France
Communication dans un congrès hal-04398166v1

Effects of pH stress during growth on the physiological, biophysical and biochemical properties of Carnobacterium maltaromaticum cells

Amélie Girardeau , Stéphanie Passot , Stéphanie Cénard , Pascale Lieben , Fernanda Fonseca
5th Microbial Stress Conference of the European Federation of Biotechnology, Nov 2020, Virtual, France
Communication dans un congrès hal-04398133v1
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Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation

Fernanda Fonseca , Julie Meneghel , Peter Kilbride , Stéphanie Passot , G. John Morris
9. International Symposium on Lyophilization of Pharmaceuticals ISLFD, Sep 2019, Ghent, Belgium
Communication dans un congrès hal-02734376v1

Development of a simplified lipid model of bacterial membrane to investigate specific interactions with oligosaccharides

Fernanda Fonseca , Michele da Silva Pinto , Julie Meneghel , Pascale Lieben , Stéphanie Passot
56. Annual Meeting of the Society for Cryobiology, Jul 2019, San Diego, CA, United States. pp.173, ⟨10.1016/j.cryobiol.2019.10.109⟩
Communication dans un congrès hal-02266911v1

Complementary analytical approaches improving knowledge on lactic acid bacteria cryoresistance

Amélie Girardeau , Julie Meneghel , Ioan-Cristian Trelea , Stéphanie Passot , Fernanda Fonseca
56. Annual Meeting of the Society for Cryobiology, Jul 2019, San Diego, CA, United States. 166-166 p., ⟨10.1016/j.cryobiol.2019.10.086⟩
Communication dans un congrès hal-02266910v1

Avancées récentes sur la production et la stabilisation de bactéries lactiques à haute fonctionnalité

Stéphanie Passot , Catherine Béal , Fernanda Fonseca
CBL 2019 - 22. Colloque du Club des Bactéries Lactiques, Jun 2019, Caen, France
Communication dans un congrès hal-02268915v1

Resistencia de L. plantarum WCFS1 frente a distintos métodos de secado: análisis de componentes celulares asociados a su degradación mediante microespectroscopía FTIR en suspensión

Maite Gagneten , Stéphanie Passot , Carolina Schebor , Fernanda Fonseca
XVIII Congreso argentino de ciencia y tecnología de alimento, Oct 2023, Buenos Aires, Argentina
Poster de conférence hal-04395365v1
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Stabilization of Lactiplantibacillus plantarum WCFS1 during freezing, freeze-drying, and storage using legume wastewater extracts

Gonçalo N. Martins , Maria Guerrero Sanchez , Angela D. Carboni , Fernanda Fonseca , Sonia Campoy
3rd Food Chemistry Conference, Oct 2023, Dresden, Germany
Poster de conférence hal-04395559v1
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Scale up of layer-by-layer microencapsulation of probiotic bacteria Lactiplantibacillus plantarum WCFS1

Gina Tavares , Nelson Romano , Stéphanie Passot , Maria Guerrero Sanchez , Marina A. Golowczyc
International Congress on Engineering and Food, Jun 2023, Nantes, France
Poster de conférence hal-04397455v1

Estudio de la estabilidad de L. bulgaricus protegido con fructooligosacáridos durante el secado y el almacenamiento

Maite Gagneten , Carolina Schebor , Stéphanie Cenard , Andrea Gomez-Zavaglia , Stéphanie Passot
VIII Congreso Internacional de Ciencia y Tecnología de Alimentos (CICYTAC 2022), Oct 2022, Cordoba, Argentina
Poster de conférence hal-04397979v1
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Identification of stressful conditions during fermentation and preservation for improving the stability of freeze-dried Lactobacillus salivarius BT621

Maria Guerrero Sánchez , Sonia Campoy García , Monica Olivares , Stephanie Passot , Fernanda Fonseca
5th Microbial Stress Conference of the European Federation of Biotechnology, Nov 2020, Virtual, France
Poster de conférence hal-04398066v1
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Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties

Fernanda Fonseca , Amélie Girardeau , Stephanie Passot
Willem F. Wolkers; Harriëtte Oldenhof. Cryopreservation and Freeze-Drying Protocols, 2180, Springer US, pp.703-719, 2021, Methods in Molecular Biology, 978-1-0716-0782-4. ⟨10.1007/978-1-0716-0783-1_38⟩
Chapitre d'ouvrage hal-03824400v1