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Giuliano Sciara

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Présentation

**Address** <a name="_Hlt476690674"></a>Laboratory “Biodiversity and Biotechnology of Fungi” BBF UMR1163 Aix-Marseille Université / INRAE - Polytech Marseille, Faculté des Sciences de Luminy - 163, Av de Luminy - 13009 Marseille, France tel: +33 6 2385 6888 / email: giuliano.sciara@inrae.fr **Personal Information** Date of birth: 10/03/1973 ========================= Place of birth: Rimini (ITALY) Nationality: Italian **Work Experience** Since 01/01/2017: **INRAE Researcher** (Chargé de Recherche). Unit: BBF - UMR1163 Aix-Marseille Université / INRAE. 01/02/2015 - 31/12/2016: **PostDoc** BBF UMR1163 Aix-Marseille Université / INRAE **-** Marseille, FRANCE (Dr. J-G. Berrin) (Characterization of Fungal Enzymes involved in Plant Cell Wall degradation). 01/04/2013 - 31/03/2014: **PostDoc (Marie Curie IOF fellow)** BIP UMR7281 Aix-Marseille Université / CNRS **-** Marseille, FRANCE (Dr. M.T. Giudici-Orticoni) (Structural Studies of Respiratory Chain Metalloproteins from Extremophiles). 01/01/2010 - 31/03/2013: **PostDoc (2011 - 13: Marie Curie IOF fellow)** Dept. of Physiology and Cellular Biophysics - Columbia University - New York, USA (Prof. F. Mancia) (Membrane Proteins: Production, Stability by high-throughput approaches and Crystallography). 01/01/2005 - 31/12/2009: **PostDoc (2006 - 07: Marie Curie EIF fellow)** AFMB UMR6098 - Aix-Marseille Université / CNRS **-** Marseille, FRANCE (Dr. C. Cambillau) (Membrane Proteins and Large Macromoleculaar Complexes: high-throughput approaches, Solution Studies by Light Scattering and Structural Studies by Crystallography). 10/02 - 30/09/2000; 01/10/2003 - 31/12/2004: **Predoctoral Internship / PostDoc** Dipart. Sc. Biochimiche - Università « La Sapienza » **-** Roma, ITALY (Prof. M. Brunori and B. Vallone) (Structural Studies of Enzymes and Globins, , Structural Investigation of Protein Conformational Changes, Mechanisms of Natural Antibiotics Biosynthesis). **Scientific Skills** \- Studied Systems: plant cell wall active Enzymes, Metalloproteins, large Macromolecular Assemblies and Membrane Proteins. \- Structural Studies: crystallization and crystallography (data collection and analysis); building and analysis of protein models; NMR. \- Solution Studies: light scattering (aggregation state, protein-protein and protein-detergent interactions and stoichiometry, protein glycosylation); analytical HPLC; spectroscopies (UV/Vis, Fluorescence, CD). \- Molecular Biology and Protein Production in *E. coli*, *A. niger* and *P. pastoris*: cloning; protein engineering; protein purification; high-throughput approaches. **Other Professional Skills** \- Scientific Writing: applications for funding and fellowships, articles, reports. \- Scientific Presentations: conferences, meetings. \- Informatics: Office, graphics packages, scientific software. Operating systems: MacOS, Linux, PC. \- Group Management and Tutoring (students and technicians). \- Project Management: strategies, problem solving, protocol design, execution. \- Lab Management: section responsibilities, platform setup and supervision. \- Languages: Italian (mother tongue); English (proficient user); French (proficient user). **Teaching and Tutoring Activities** \- Since 2005: (co-)tutoring of technicians, bachelor, master and PhD students. \- 2015-16: fixed-term lecturer for master (BBSG, 1st and 2nd year) and bachelor courses (Aix-Marseille Université): lectures on protein crystallography. \- 2003-2004: teaching activities for the Chemistry and Introduction to Biochemistry Course (Medicine Faculty “La Sapienza”): tutorials and laboratory classes (titrations, buffer systems, protein precipitation at their pI); member of examination committees; ADE “Elective Didactic Activity” (lecture on radioactivity and its applications in medicine). **Fellowships and Distinctions** 2011: Postdoctoral Fellowship (3 years) : Marie Curie International Outgoing Fellowship (IOF) 2006: Postdoctoral Fellowship (2 years) : Marie Curie Intra European Fellowship (EIF) 2004: PhD Thesis Prize « Mario Rippa - 2003/2004 » 1995: ERASMUS Fellowship (3 months) : Laboratory for Crystallography, Universiteit van Amsterdam – NETHERLANDS (Prof. H. Schenk) **Education and Qualifications** 2015: **Qualification Maitre de Conférence** (equivalent to “Abilitazione Prof. Associato”) - Ministère de L'Education Française - Section 64: « Biochemistry and Molecular Biology ». 2004: **Esame di Stato** (Biologo) - Ministero Italiano dell’Istruzione. 2004: **PhD in Biochemistry** - Dipart. Sc. Biochimiche - Università « La Sapienza » **-** Roma, ITALY (Prof. M. Brunori and B. Vallone) PhD Thesis: « The crystal structure at 1.3 Å resolution of ActVA-orf6 from *Streptomyces coelicolor*, a monooxygenase without cofactors ». 1999: **Master Degree in Biology** (Laurea cum laude) Dipart. Chimica Agraria - Università di Bologna **-** Bologna, ITALY (Prof. S. Ciurli) Master Thesis: « Solution structure of oxidized cytochrome *c*553 from *B. pasteurii* through NMR spectroscopy ». 1987 - 92: **Italian high school:** Liceo Scientifico “A. Volta”, Riccione – ITALY. 1987 - 92: **Music high school:** Conservatorio “G. Rossini”, Pesaro – ITALY.

Publications

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Beyond the coupled distortion model: structural analysis of the single domain cupredoxin AcoP, a green mononuclear copper centre with original features

Magali Roger , Philippe Leone , Ninian J Blackburn , Sam Horrell , Tadeo Moreno Chicano
Dalton Transactions, 2024, 53, pp.1794 - 1808. ⟨10.1039/d3dt03372d⟩
Article dans une revue hal-04417309v1
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Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccase

Sona Malric-Garajova , Florian Fortuna , Florian Pion , Elise Martin , Adithya Raveendran Thottathil
Molecules, 2023, 28 (12), pp.4873. ⟨10.3390/molecules28124873⟩
Article dans une revue hal-04228332v1

Effect of laccase pre-treatment on the mechanical properties of lignin-based agrocomposites reinforced with wood fibers

Elise Martin , Eric Badel , Stéphanie Léger , Pascal Dubessay , Cedric Delattre
Industrial Crops and Products, 2022, 189, pp.115876. ⟨10.1016/j.indcrop.2022.115876⟩
Article dans une revue hal-04055690v1
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Optimization of the Decolorization of the Reactive Black 5 by a Laccase-like Active Cell-Free Supernatant from Coriolopsis gallica

Amal Ben Ayed , Bilel Hadrich , Giuliano Sciara , Anne Lomascolo , Emmanuel Bertrand
Microorganisms, 2022, 10 (6), pp.1137. ⟨10.3390/microorganisms10061137⟩
Article dans une revue hal-03684201v1
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Biotransformation of the fluoroquinolone, levofloxacin, by the white-rot fungus $Coriolopsis\ gallica$

Amal Ben Ayed , Imen Akrout , Quentin Albert , Stéphane Greff , Charlotte Simmler
Journal of Fungi, 2022, 8 (9), pp.965. ⟨10.3390/jof8090965⟩
Article dans une revue hal-03780697v1
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The Maize Pathogen Ustilago maydis Secretes Glycoside Hydrolases and Carbohydrate Oxidases Directed toward Components of the Fungal Cell Wall

Jean‐lou Reyre , Sacha Grisel , Mireille Haon , David Navarro , David Ropartz
Applied and Environmental Microbiology, 2022, 88 (23), pp.1-16. ⟨10.1128/aem.01581-22⟩
Article dans une revue hal-03885449v1
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Exploring the Diversity of Fungal DyPs in Mangrove Soils to Produce and Characterize Novel Biocatalysts

Amal Ben Ayed , Geoffroy Saint-Genis , Laurent Vallon , Dolores Linde , Annick Turbé-Doan
Journal of Fungi, 2021, 7 (5), pp.321. ⟨10.3390/jof7050321⟩
Article dans une revue hal-03215284v2
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Screening New Xylanase Biocatalysts from the Mangrove Soil Diversity

Corinne Ivaldi , Mariane Daou , Laurent Vallon , Alexandra Bisotto , Mireille Haon
Microorganisms, 2021, 9 (7), pp.1-16. ⟨10.3390/microorganisms9071484⟩
Article dans une revue insu-03293424v1

Treatment of wood fbres with laccases: improved hardboard properties through phenolic oligomerization

Sona Garajova , Ilabahen Patel , Anne Lomascolo , Frédéric F. Legée , Laurent L. Cezard
European Journal of Wood and Wood Products, 2021
Article dans une revue hal-03512563v1
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Crystal structure and functional characterization of an oligosaccharide dehydrogenase from Pycnoporus cinnabarinus provides insights into fungal breakdown of lignocellulose

Gabriele Cerutti , Elena Gugole , Linda Celeste Montemiglio , Annick Turbé-Doan , Dehbia Chena
Biotechnology for Biofuels, 2021, 14, pp.161. ⟨10.1186/s13068-021-02003-y⟩
Article dans une revue hal-03344334v1

Treatment of wood fibres with laccases: improved hardboard properties through phenolic oligomerization

Soňa Garajová , Ilabahen Patel , Anne Lomascolo , Frédéric Legée , Laurent Cézard
European Journal of Wood and Wood Products, 2021, ⟨10.1007/s00107-021-01720-3⟩
Article dans une revue hal-03303387v1
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Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

Wissal Ben Ali , Delphine Chaduli , David Navarro , Christian Lechat , Annick Turbé-Doan
BMC Biotechnology, 2020, 20 (1), pp.27. ⟨10.1186/s12896-020-00617-y⟩
Article dans une revue hal-02928294v1
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Characterization of the CAZy Repertoire from the Marine-Derived Fungus Stemphylium lucomagnoense in Relation to Saline Conditions

Wissal Ben Ali , David Navarro , Abhishek Kumar , Elodie Drula , Annick Turbé-Doan
Marine drugs, 2020, 18 (9), pp.1-21. ⟨10.3390/md18090461⟩
Article dans une revue hal-02953538v1

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

Wissal Ben Ali , Delphine Chaduli , David Navarro , Christian Lechat , Annick Turbé-Doan
BMC Biotechnology, 2020, 20 (1), ⟨10.1186/s12896-020-00617-y⟩
Article dans une revue hal-03512407v1
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Enzyme Properties of a Laccase Obtained from the Transcriptome of the Marine-Derived Fungus Stemphylium lucomagnoense

Wissal Ben Ali , Amal Ben Ayed , Annick Turbé-Doan , Emmanuel Bertrand , Yann Mathieu
International Journal of Molecular Sciences, 2020, 21 (21), pp.8402. ⟨10.3390/ijms21218402⟩
Article dans une revue hal-03000290v1
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Characterization and Dye Decolorization Potential of Two Laccases from the Marine-Derived Fungus Pestalotiopsis sp.

Saowanee Wikee , Juliette Hatton , Annick Turbé-Doan , Yann Mathieu , Marianne Daou
International Journal of Molecular Sciences, 2019, 20 (8), pp.1864. ⟨10.3390/ijms20081864⟩
Article dans une revue hal-02276821v1

Impact of copper ligand mutations on a cupredoxin with a green copper center.

Magali Roger , Giuliano Sciara , Frédéric Biaso , Elisabeth Lojou , Xie Wang
BBA - Biochimica et Biophysica Acta, 2017, 1858 (5), pp.351-359. ⟨10.1016/j.bbabio.2017.02.007⟩
Article dans une revue hal-01481398v1

Functional analysis and crystallographic structure of clotrimazole bound OleP, a cytochrome P450 epoxidase from Streptomyces antibioticus involved in oleandomycin biosynthesis.

Linda Celeste Montemiglio , Giacomo Parisi , Antonella Scaglione , Giuliano Sciara , Carmelinda Savino
Biochimica et Biophysica Acta (BBA) - General Subjects, 2016, 1860 (3), pp.465-475. ⟨10.1016/j.bbagen.2015.10.009⟩
Article dans une revue hal-01581613v1
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Spectroscopic Characterization of a Green Copper Site in a Single-Domain Cupredoxin

Magali Roger , Frédéric Biaso , Cindy J. Castelle , Marielle Bauzan , Florence Chaspoul
PLoS ONE, 2014, 9 (6), pp.e98941. ⟨10.1371/journal.pone.0098941⟩
Article dans une revue hal-01494439v1
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Structural basis for catalysis in a CDP-alcohol phosphotransferase.

Giuliano Sciara , Oliver B Clarke , David Tomasek , Brian Kloss , Shantelle Tabuso
Nature Communications, 2014, 5, pp.4068. ⟨10.1038/ncomms5068⟩
Article dans une revue hal-01594683v1

Highlights from recently determined structures of membrane proteins: a focus on channels and transporters.

Giuliano Sciara , Filippo Mancia
Current Opinion in Structural Biology, 2012, 22 (4), pp.476-81. ⟨10.1016/j.sbi.2012.03.005⟩
Article dans une revue hal-01615073v1

Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1.

Kerstin Michalke , Céline Huyghe , Julie Lichière , Marie-Eve Gravière , Marina Siponen
Analytical Biochemistry, 2010, 401 (1), pp.74-80. ⟨10.1016/j.ab.2010.02.017⟩
Article dans une revue hal-01595269v1

Structure of lactococcal phage p2 baseplate and its mechanism of activation.

Giuliano Sciara , Cecilia Bebeacua , Patrick Bron , Denise Tremblay , Miguel Ortiz-Lombardia
Proceedings of the National Academy of Sciences of the United States of America, 2010, 107 (15), pp.6852-7. ⟨10.1073/pnas.1000232107⟩
Article dans une revue hal-01595268v1

Solution and electron microscopy characterization of lactococcal phage baseplates expressed in Escherichia coli.

Valérie Campanacci , David Veesler , Julie Lichière , Stéphanie Blangy , Giuliano Sciara
Journal of Structural Biology, 2010, 172 (1), pp.75-84. ⟨10.1016/j.jsb.2010.02.007⟩
Article dans une revue hal-01595270v1

Pattern of cavities in globins: the case of human hemoglobin.

Carmelinda Savino , Adriana E Miele , Federica Draghi , Kenneth A Johnson , Giuliano Sciara
Biopolymers, 2009, 91 (12), pp.1097-1107. ⟨10.1002/bip.21201⟩
Article dans une revue hal-01617675v1

Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone.

Marina Siponen , Giuliano Sciara , Manuela Villion , Silvia Spinelli , Julie Lichière
Journal of Bacteriology, 2009, 191 (3), pp.728-34. ⟨10.1128/JB.01363-08⟩
Article dans une revue hal-01615930v1

Surfactant Poloxamer 188 as a New Crystallizing Agent for Urate Oxidase

Marion Giffard , Vanessa Delfosse , Giuliano Sciara , Claudine Mayer , Christian Cambillau
Crystal Growth & Design, 2009, 9 (9), pp.4199-4206. ⟨10.1021/cg900473n⟩
Article dans une revue hal-01617674v1

Production and biophysical characterization of the CorA transporter from Methanosarcina mazei.

David Veesler , Stéphanie Blangy , Marina Siponen , Renaud Vincentelli , Christian Cambillau
Analytical Biochemistry, 2009, 388 (1), pp.115-21. ⟨10.1016/j.ab.2009.02.018⟩
Article dans une revue hal-01615932v1
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Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.

Carmelinda Savino , Linda C Montemiglio , Giuliano Sciara , Adriana E Miele , Steven G Kendrew
Journal of Biological Chemistry, 2009, 284 (42), pp.29170-9. ⟨10.1074/jbc.M109.003590⟩
Article dans une revue hal-01617673v1

A Topological Model of the Baseplate of Lactococcal Phage Tuc2009

Giuliano Sciara , Stéphanie Blangy , Marina I Siponen , Stephen Mc Grath , Douwe van Sinderen
Journal of Biological Chemistry, 2008, 283 (5), pp.2716-2723. ⟨10.1074/jbc.M707533200⟩
Article dans une revue hal-02066265v1

There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization.

David Veesler , Stéphanie Blangy , Christian Cambillau , Giuliano Sciara
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2008, 64 (Pt 10), pp.880-885. ⟨10.1107/S1744309108028248⟩
Article dans une revue hal-01617665v1

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of C-12 hydroxylase EryK from Saccharopolyspora erythraea.

Carmelinda Savino , Giuliano Sciara , Adriana E Miele , Steven G Kendrew , Beatrice Vallone
Protein and Peptide Letters, 2008, 15 (10), pp.1138-1141. ⟨10.2174/092986608786071201⟩
Article dans une revue hal-01617667v1

Extended subnanosecond structural dynamics of myoglobin revealed by Laue crystallography.

Dominique Bourgeois , Beatrice Vallone , Alessandro Arcovito , Giuliano Sciara , Friedrich Schotte
Proceedings of the National Academy of Sciences of the United States of America, 2006, 103 (13), pp.4924-4929. ⟨10.1073/pnas.0508880103⟩
Article dans une revue hal-01617679v1

Complex landscape of protein structural dynamics unveiled by nanosecond Laue crystallography.

Dominique Bourgeois , Beatrice Vallone , Friedrich Schotte , Alessandro Arcovito , Adriana E Miele
Proceedings of the National Academy of Sciences of the United States of America, 2003, 100 (15), pp.8704-9. ⟨10.1073/pnas.1430900100⟩
Article dans une revue hal-01595186v1

Analysis of the effect of microgravity on protein crystal quality: the case of a myoglobin triple mutant.

Adriana E Miele , Luca Federici , Giuliano Sciara , Federica Draghi , Maurizio Brunori
Acta crystallographica Section D : Structural biology [1993-..], 2003, 59 (6), pp.982-928
Article dans une revue hal-01595187v1

The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.

Giuliano Sciara , Steven G Kendrew , Adriana E Miele , Neil G Marsh , Luca Federici
EMBO Journal, 2003, 22 (2), pp.205-15. ⟨10.1093/emboj/cdg031⟩
Article dans une revue hal-01595326v1

NMR Solution Structure, Backbone Mobility, and Homology Modeling ofc-Type Cytochromes from Gram-Positive Bacteria

Lucia Banci , Ivano Bertini , Stefano Ciurli , Alexander Dikiy , Jens Dittmer
Article dans une revue hal-03259473v1