Hervé LE HIR

46
Documents

Présentation

CURRENT POSITION Research Director, 1st class - CNRS

Head of IBENS section "Functional Genomics"

Group leader, team "Expression of eukaryotic mRNAs"

Institut de Biologie de l’Ecole Normale Supérieure (IBENS)–

UMR8197-U1024. 46 rue d’Ulm, 75005, Paris

EDUCATION 2006 : HDR, University Paris-Sud Orsay (Paris 11)

1998: PhD, Molecular Biology, University Paris Diderot (Paris 7)

1993 : Master (B.A.) Biochemistry, UPMC (Paris 6)

DISTINCTION 2009 :ATIP and ATIP+ CNRS

2010, 2014, 2019 : Prime d’Encadrement Doctoral et de Recherche CNRS

RESEARCH EXPERIENCE

Since 2009: Group leader of the team "Expression of eukaryotic mRNAs"

IBENS – Section 2, Functional Genomics – Paris

2001-2008: CR2/1 CNRS in the group of Dr. Bertrand Séraphin

CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France.

1998-2001: Post-doctoral fellow in the lgroups of Dr. Lynne Maquat (Roswell Park Cancer Institute / Buffalo USA) and of Pr. Melissa J. Moore (HHMI / Brandeis University, MA. USA)

1993-1998: Ph.D. (PI: Dr. Claude Thermes) – University Paris 7

CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France.

FUNDING

2018-2021: ANR blanche – spEJCificity (Project leader)

2015-2018: ANR blanche – CleaNMD (Partner, coordinator Alain Jacquier)

2014-2017: ANR blanche – DifferEJnCe (Project leader)

2014-2015: PSL research – (Partner, coordinator Stephan Vagner)

2014-2015: Association Recherche contre le Cancer – (Project leader)

2012-2015: FP7 Marie Curie Initial Training Network « RNPnet »

2012-2013: Fondation P.G. de Gennes (Partner)

2011-2013: ANR blanche – EJCbirth (Project leader)

2010-2013: Equipe FRM (Project leader)

2010: Installation Nouvelle Equipe FRM

2010-2011: Ligue Nationale Contre le Cancer (Project leader)

2009-2011: ANR blanche – Explore EJC (Project leader)

2009-2011: ATIP CNRS

2005-2008: ANR blanche – L’EJC (Project leader)

2003-2006: A.C.I. Jeunes Chercheurs

2003-2004: France-Berkeley Fund

Supervision activities

1 Researcher (CR INSERM, J. Saulière)

6 PhD students (B. Marchadier, PE Chazal, R. Mishra, J. Kanaan, V. Busetto, J. Paternina)

5 Post-docs (C. Barrandon, F. Fiorini, Z. Wang, E. Marquenet, O.S. Kwon)

7 Engineers (F. Bonneau, V. Murigneux, L. Bastianelli, R. Servignat d’Aout, R. Hocq, Q.Alasseur, J. Paternina) 1 technician CNRS (I. Barbosa).

9 Master students

COMMUNICATIONS

Teaching activities

Since 2009: Master in Genetics, UPMC (3h per year)

Since 2009: ENS L3 Chimie (2h per year)

Since 2009: ENS Master in Genetics, UPMC (3h per year)

2014: PhD program, Wellcome Trust Centre for Cell Biology, Edinburgh, UK (2014)

2014: PhD program, FMI, Basel, Switzerland (2014)

2012: PhD program, University Toulouse (2012)

2017: Co-organizer and teaching: International course Curie-ENS “Posttranscriptional

gene regulation” (2017)

Peer review and expertise activity

• Reviewer for several journals including: Cell; Nature; Nature Structural & Molecular Biology; Genes & Development; EMBO Journal; Journal of Cell Biology; Nucleic Acids Research; P.N.A.S.; Cell Report.

• Reviewer for several grant agencies including: ERC; Wellcome Trust; ANR; Ligue

contre le Cancer; Foreign agencies (Denmark, Poland, Israel, etc…).

• Since 2013: Member executive council doctoral school “Complexité du Vivant” – University UPMC (Paris 6).

• Since 2015: Member scientifc council “Ligue Grand Est”

• 2013-2016: Member scientifc council “Fondation Pierre-Gilles de Gennes”

• 2014: Vice-President ANR committee CES10

• 2016: Member ANR committee CES12 • 2017-2020: President ANR committee CES12

SELECTED PUBLICATIONS

Kanaan Hocq R, Paternina J, Alasseur Q, Genovesio A, Le Hir H. (2018) Monitored eCLIP: high accuracy mapping of RNA-protein interactions. Nucleic Acids Research 2018 Nov;46(21):11553-11565.

Kanaan J, Raj S, Decourty L, Saveanu C, Croquette V, Le Hir H. (2018) UPF1-like helicase grip on nucleic acids dictates processivity. Nature Communications. 2018 Sep;14(9):3752.

Le Hir H, Saulière J, Wang Z. (2016) The exon junction complex as a node of postranscriptional networks. Nature Review in Molecular and Cellular Biology. 2016 Jan;17(1):41-54.

Fiorini F, Bagchi D, Le Hir H*, Croquette V*. (2015) Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities. Nature Communications. Jul 3;6:7581. *Corresponding authors

Wang Z, Murigneux V, Le Hir H. (2014). Transcriptome-wide modulation of splicing by the Exon Junction Complex. Genome Biology. 15(12):551.

Chazal PE, Daguenet E, Wendling C, Ulrycke N, Tomasetto C, Sargueil B, Le Hir H (2013). EJC core component MLN51 interacts with eIF3 and activates translation. P.N.A.S. 110(15):5903-8.

Saulière J, Murigneux V, Wang Z, Marquenet E, Barbosa I, Le Tonquèze O, Audic Y, Paillard L, Roest Crollius H, Le Hir H. (2012). CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex. Nature Structural and Molecular Biology 19(11):1124-31.

Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, Le Hir H. (2012). Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly. Nature Structural and Molecular Biology 19(10):983-90.

Chakrabarti S, Jayachandran U, Bonneau F, Fiorini F, Basquin C, Domcke S, Le Hir H, Conti E. (2011). Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and Its Regulation by Upf2. Molecular Cell 41(6):682-692.

Saulière J, Haque N, Harms S, Barbosa I, Blanchette M, Le Hir H. (2010). The exon junction complex differentially marks spliced junctions. Nature Structural and Molecular Biology 17(10):1269-71.

Le Hir H, Séraphin B. (2008). EJCs at the heart of translational control. Cell. 133(2):213-6.

Chamieh H, Ballut L, Bonneau F, Le Hir H. (2008). NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity. Nature Structural and Molecular Biology 15(1):85-93.

Andersen CB*, Ballut L*, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H#, Andersen GR#. (2006). Structure of the Exon Junction Core Complex with a Trapped DEAD-Box ATPase Bound to RNA. Science 313:1968-1972.

Ballut L, Marchadier B, Baguet A, Tomasetto C, Séraphin B, Le Hir H. (2005). The Exon-Junction complex is locked onto mRNA by inhibition of eIF4A3 ATPase activity. Nature Structural and Molecular Biology 12(10):861-9.

Le Hir, H., Gatfield, D., Izaurralde, E. & Moore, M.J. (2001). The exon-exon junction complex provides a binding platform for factors involved in mRNA export and NMD. The EMBO Journal 20 (17):4987-4997.

Le Hir, H., Izaurralde, E., Maquat, L.E. & Moore, M.J. (2000). The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. The EMBO Journal 19 (24):6860-6869.

Publications

Publications

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Co-translational determination of quaternary structures in chaperone factories

Manon Philippe , Soha Salloum , Floric Slimani , Davide Normanno , Isabelle Barbosa et al.

Nature Communications, 2026, 17 (1), pp.1978. ⟨10.1038/s41467-026-68687-8⟩

Article dans une revue hal-05482009v2
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RNA anchoring of Upf1 facilitates recruitment of Dcp2 in the NMD decapping complex

Nadia Ruiz-Gutierrez , Jeanne Dupas , Elvire Auquier , Irène Barbarin-Bocahu , Claudine Gaudon-Plesse et al.

Nucleic Acids Research, 2025, 53 (5), pp.gkaf160. ⟨10.1093/nar/gkaf160⟩

Article dans une revue hal-05051133v1
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Polysome sorting controls mRNA localization and protein fate

Adham Safieddine , Jonathan Bizarro , Soha Salloum , Hervé Le Hir , Edouard Bertrand

Trends in Cell Biology, 2025, ⟨10.1016/j.tcb.2025.08.005⟩

Article dans une revue hal-05371249v1
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A low-level Cdkn1c/p57kip2 expression in spinal progenitors drives the transition from proliferative to neurogenic modes of division

Baptiste Mida , Nathalie Lehmann , Rosette Goïame , Fanny Coulpier , Kamal Bouhali et al.

EMBO Reports, 2025, ⟨10.1038/s44319-025-00653-9⟩

Article dans une revue hal-05426812v1
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Chemical inhibition of exon junction complex assembly impairs mRNA localization and neural stem cells ciliogenesis

Tommaso Villa , Oriane Pourcelot , David Dierks , Marion Faucourt , Cindy Burel et al.

Nucleic Acids Research, 2025, 53 (51), pp.gkaf1200. ⟨10.1093/nar/gkaf1200⟩

Article dans une revue hal-05376224v1
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Structure of the Nmd4-Upf1 complex supports conservation of the nonsense-mediated mRNA decay pathway between yeast and humans

Irène Barbarin-Bocahu , Nathalie Ulryck , Amandine Rigobert , Nadia Ruiz Gutierrez , Laurence Decourty et al.

PLoS Biology, 2024, 22 (9), pp.e3002821. ⟨10.1371/journal.pbio.3002821⟩

Article dans une revue hal-04758977v1
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Exon-junction complex association with stalled ribosomes and slow translation-independent disassembly

Olivier Bensaude , Isabelle Barbosa , Lucia Morillo , Rivka Dikstein , Hervé Le Hir

Nature Communications, 2024, 15 (1), pp.4209. ⟨10.1038/s41467-024-48371-5⟩

Article dans une revue hal-04758965v1
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Exclusion of m6A from splice-site proximal regions by the exon junction complex dictates m6A topologies and mRNA stability

Anna Uzonyi , David Dierks , Ronit Nir , Oh Sung Kwon , Ursula Toth et al.

Molecular Cell, 2023, 83 (2), pp.237-251.e7. ⟨10.1016/j.molcel.2022.12.026⟩

Article dans une revue hal-04291793v1
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Comprehensive mapping of exon junction complex binding sites reveals universal EJC deposition in Drosophila

Lucía Morillo , Toni Paternina , Quentin Alasseur , Auguste Genovesio , Schraga Schwartz et al.

BMC Biology, 2023, 21 (1), pp.246. ⟨10.1186/s12915-023-01749-1⟩

Article dans une revue hal-04291789v1
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A choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport

Adham Safieddine , Emeline Coleno , Soha Salloum , Arthur Imbert , Abdel-Meneem Traboulsi et al.

Nature Communications, 2021, 12 (1), pp.1352. ⟨10.1038/s41467-021-21585-7⟩

Article dans une revue hal-03014936v2
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Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis

Oh Sung Kwon , Rahul Mishra , Adham Safieddine , Emeline Coleno , Quentin Alasseur et al.

Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-021-21590-w⟩

Article dans une revue hal-03382897v3
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A role for AKT1 in nonsense-mediated mRNA decay

Martine Palma , Catherine Leroy , Sophie Salomé-Desnoulez , Elisabeth Werkmeister , Rebekah Kong et al.

Nucleic Acids Research, 2021, 49 (19), pp.11022-11037. ⟨10.1093/nar/gkab882⟩

Article dans une revue hal-03423347v1

Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes

Virginia Busetto , Isabelle Barbosa , Jérôme Basquin , Émelie Marquenet , Rémi Hocq et al.

Nucleic Acids Research, 2020, 48 (10), pp.5670-5683. ⟨10.1093/nar/gkaa267⟩

Article dans une revue hal-03419075v1
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A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting

Racha Chouaib , Adham Safieddine , Xavier Pichon , Arthur Imbert , Oh Sung Kwon et al.

Developmental Cell, 2020, 54 (6), pp.773 - 791.e5. ⟨10.1016/j.devcel.2020.07.010⟩

Article dans une revue pasteur-02925687v1
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Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes

Virginia Busetto , Isabelle Barbosa , Jérôme Basquin , Emélie Marquenet , Rémi Hocq et al.

Nucleic Acids Research, 2020, 48 (10), pp.5670-5683. ⟨10.1093/nar/gkaa267⟩

Article dans une revue pasteur-02862504v1
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NCBP3 positively impacts mRNA biogenesis

Yuhui Dou , Isabelle Barbosa , Hua Jiang , Claudia Iasillo , Kelly Molloy et al.

Nucleic Acids Research, 2020, 48 (18), pp.10413-10427. ⟨10.1093/nar/gkaa744⟩

Article dans une revue hal-03033173v1
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ALFA: annotation landscape for aligned reads

Mathieu Bahin , Benoit Noel , Valentine Murigneux , Charles Bernard , Leila Bastianelli et al.

BMC Genomics, 2019, 20 (250), pp.1-11. ⟨10.1186/s12864-019-5624-2⟩

Article dans une revue hal-02084432v1
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Nonsense‐mediated mRNA decay involves two distinct Upf1‐bound complexes

Marine Dehecq , Laurence Decourty , Abdelkader Namane , Caroline Proux , Joanne Kanaan et al.

EMBO Journal, 2018, 37, pp.e99278. ⟨10.15252/embj.201899278⟩

Article dans une revue pasteur-01889054v1
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HTLV-1 Tax plugs and freezes UPF1 helicase leading to nonsense-mediated mRNA decay inhibition

Francesca Fiorini , Jean-Philippe Robin , Joanne Kanaan , Malgorzata Borowiak , Vincent Croquette et al.

Nature Communications, 2018, 9 (1), ⟨10.1038/s41467-017-02793-6⟩

Article dans une revue hal-02358887v1

Exon Junction Complexes can have distinct functional flavours to regulate specific splicing events

Zhen Wang , Lionel Ballut , Isabelle Barbosa , Hervé Le Hir

Scientific Reports, 2018, 8 (1), pp.9509. ⟨10.1038/s41598-018-27826-y⟩

Article dans une revue hal-04988943v1
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Monitored eCLIP: high accuracy mapping of RNA-protein interactions

Rémi Hocq , Janio Paternina , Quentin Alasseur , Auguste Genovesio , Hervé Le Hir

Nucleic Acids Research, 2018, 46 (21), pp.11553-11565. ⟨10.1093/nar/gky858⟩

Article dans une revue hal-02387433v1
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UPF1-like helicase grip on nucleic acids dictates processivity

Joanne Kanaan , Saurabh Raj , Laurence Decourty , Cosmin Saveanu , Vincent Croquette et al.

Nature Communications, 2018, 9 (1), pp.3752. ⟨10.1038/s41467-018-06313-y⟩

Article dans une revue hal-01878957v1
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A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner

Manjeera Gowravaram , Fabien Bonneau , Joanne Kanaan , Vincent D Maciej , Francesca Fiorini et al.

Nucleic Acids Research, 2018, 46 (5), pp.2648 - 2659. ⟨10.1093/nar/gky040⟩

Article dans une revue hal-01762546v1

A mechanistic study of helicases with magnetic traps

Samar Hodeib , Saurabh Raj , Maria Manosas , Weiting Zhang , Debjani Bagchi et al.

Protein Science, 2017, 26 (7), pp.1314-1336. ⟨10.1002/pro.3187⟩

Article dans une revue hal-02358906v1
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Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities

Francesca Fiorini , Debjani Bagchi , Hervé Le Hir , Vincent Croquette

Nature Communications, 2015, 6 (1), pp.7581. ⟨10.1038/ncomms8581⟩

Article dans une revue hal-02358918v1

Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains

Francesca Fiorini , Marc Boudvillain , Hervé Le Hir

Nucleic Acids Research, 2013, 41 (4), pp.2404-2415. ⟨10.1093/nar/gks1320⟩

Article dans une revue hal-02358926v1

EJC core component MLN51 interacts with eIF3 and activates translation

Pierre-Etienne Chazal , Elisabeth Daguenet , Corinne Wendling , Nathalie Ulryck , Catherine Tomasetto et al.

Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 (15), pp.5903-5908. ⟨10.1073/pnas.1218732110⟩

Article dans une revue hal-04211808v1

CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex.

Jérôme Saulière , Valentine Murigneux , Zhen Wang , Emélie Marquenet , Isabelle Barbosa et al.

Nature Structural and Molecular Biology, 2012, 19 (11), pp.1124-31. ⟨10.1038/nsmb.2420⟩

Article dans une revue istex hal-00982626v1
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Perispeckles are major assembly sites for the exon junction core complex.

Elisabeth Daguenet , Aurélie Baguet , Sébastien Degot , Ute Schmidt , Fabien Alpy et al.

Molecular Biology of the Cell, 2012, 23 (9), pp.1765-1782. ⟨10.1091/mbc.E12-01-0040⟩

Article dans une revue hal-00727654v1

Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly

Isabelle Barbosa , Nazmul Haque , Francesca Fiorini , Charlotte Barrandon , Catherine Tomasetto et al.

Nature Structural and Molecular Biology, 2012, 19 (10), pp.983-990. ⟨10.1038/nsmb.2380⟩

Article dans une revue istex hal-02358933v1

The Human T-Lymphotropic Virus Type 1 Tax Protein Inhibits Nonsense-Mediated mRNA Decay by Interacting with INT6/EIF3E and UPF1

Vincent Mocquet , Julia Neusiedler , Francesca Rende , David Cluet , Jean-Philippe Robin et al.

Journal of Virology, 2012, 86 (14), pp.7530-7543. ⟨10.1128/JVI.07021-11⟩

Article dans une revue (data paper) hal-04897285v1

Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and Its Regulation by Upf2

Sutapa Chakrabarti , Uma Jayachandran , Fabien Bonneau , Francesca Fiorini , Claire Basquin et al.

Molecular Cell, 2011, 41 (6), pp.693-703. ⟨10.1016/j.molcel.2011.02.010⟩

Article dans une revue hal-02358974v1

NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity

Hala Chamieh , Lionel Ballut , Fabien Bonneau , Hervé Le Hir

Nature Structural and Molecular Biology, 2007, 15 (1), pp.85-93. ⟨10.1038/nsmb1330⟩

Article dans une revue istex hal-04988921v1

Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies.

Karine Buchet-Poyau , Julien Courchet , Hervé Le Hir , Bertrand Séraphin , Jean-Yves Scoazec et al.

Nucleic Acids Research, 2007, 35 (4), pp.1289-300. ⟨10.1093/nar/gkm016⟩

Article dans une revue hal-00167447v1
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The exon-junction-complex-component metastatic lymph node 51 functions in stress-granule assembly.

Aurélie Baguet , Sébastien Degot , Nicolas Cougot , Edouard Bertrand , Marie-Pierre Chenard et al.

Journal of Cell Science, 2007, 120 (Pt 16), pp.2774-84. ⟨10.1242/jcs.009225⟩

Article dans une revue hal-00189164v1

Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.

Christian B F Andersen , Lionel Ballut , Jesper S Johansen , Hala Chamieh , Klaus H Nielsen et al.

Science, 2006, 313 (5795), pp.1968-72. ⟨10.1126/science.1131981⟩

Article dans une revue hal-00168014v1

The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity.

Lionel Ballut , Brice Marchadier , Aurélie Baguet , Catherine Tomasetto , Bertrand Séraphin et al.

Nature Structural and Molecular Biology, 2005, 12 (10), pp.861-9. ⟨10.1038/nsmb990⟩

Article dans une revue istex hal-00187421v1

Expression of GFRα1 receptor splicing variants with different biochemical properties is modulated during kidney development

Nicolas Charlet-Berguerand , Hervé Le Hir , Mariarosaria Incoronato , Umberto Di Porzio , Yanbin Yu et al.

Cellular Signalling, 2004, 16 (12), pp.1425-1434. ⟨10.1016/j.cellsig.2004.05.006⟩

Article dans une revue istex hal-03339582v1

5′-End RET Splicing: Absence of Variants in Normal Tissues and Intron Retention in Pheochromocytomas

Hervé Le Hir , Nicolas Charlet-Berguerand , Vittorio de Franciscis , Claude Thermes

Oncology, 2002, 63 (1), pp.84-91. ⟨10.1159/000065725⟩

Article dans une revue hal-03339610v1

Relative Expression of the RET9 and RET51 Isoforms in Human Pheochromocytomas

Hervé Le Hir , Nicolas Charlet-Berguerand , Anne-Paule Gimenez-Roqueplo , Massimo Mannelli , Pierre-François Plouin et al.

Oncology, 2000, 58 (4), pp.311-318. ⟨10.1159/000012118⟩

Article dans une revue hal-03339608v1

Single molecule imaging reveals a conserved family of centrosomal mRNAs and a novel translation-dependent localization mechanism

Adham Safieddine , Emeline Coleno , S. Salloum , A. Imbert , Oh Sung Kwon et al.

2022 ASCB Annual Meeting - Cell Bio Virtual 2022, Dec 2022, Washington (DC), United States. , Molecular Biology of the Cell, 34 (2), pp.B298/P1290, 2023

Poster de conférence hal-05484086v1