Hervé LE HIR
Présentation
CURRENT POSITION Research Director, 1st class - CNRS Head of IBENS section "Functional Genomics" Group leader, team "Expression of eukaryotic mRNAs" Institut de Biologie de l’Ecole Normale Supérieure (IBENS)– UMR8197-U1024. 46 rue d’Ulm, 75005, Paris EDUCATION 2006 : HDR, University Paris-Sud Orsay (Paris 11) 1998: PhD, Molecular Biology, University Paris Diderot (Paris 7) 1993 : Master (B.A.) Biochemistry, UPMC (Paris 6) DISTINCTION 2009 :ATIP and ATIP+ CNRS 2010, 2014, 2019 : Prime d’Encadrement Doctoral et de Recherche CNRS RESEARCH EXPERIENCE Since 2009: Group leader of the team "Expression of eukaryotic mRNAs" IBENS – Section 2, Functional Genomics – Paris 2001-2008: CR2/1 CNRS in the group of Dr. Bertrand Séraphin CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France. 1998-2001: Post-doctoral fellow in the lgroups of Dr. Lynne Maquat (Roswell Park Cancer Institute / Buffalo USA) and of Pr. Melissa J. Moore (HHMI / Brandeis University, MA. USA) 1993-1998: Ph.D. (PI: Dr. Claude Thermes) – University Paris 7 CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France. FUNDING 2018-2021: ANR blanche – spEJCificity (Project leader) 2015-2018: ANR blanche – CleaNMD (Partner, coordinator Alain Jacquier) 2014-2017: ANR blanche – DifferEJnCe (Project leader) 2014-2015: PSL research – (Partner, coordinator Stephan Vagner) 2014-2015: Association Recherche contre le Cancer – (Project leader) 2012-2015: FP7 Marie Curie Initial Training Network « RNPnet » 2012-2013: Fondation P.G. de Gennes (Partner) 2011-2013: ANR blanche – EJCbirth (Project leader) 2010-2013: Equipe FRM (Project leader) 2010: Installation Nouvelle Equipe FRM 2010-2011: Ligue Nationale Contre le Cancer (Project leader) 2009-2011: ANR blanche – Explore EJC (Project leader) 2009-2011: ATIP CNRS 2005-2008: ANR blanche – L’EJC (Project leader) 2003-2006: A.C.I. Jeunes Chercheurs 2003-2004: France-Berkeley Fund 1 Researcher (CR INSERM, J. Saulière) 6 PhD students (B. Marchadier, PE Chazal, R. Mishra, J. Kanaan, V. Busetto, J. Paternina) 5 Post-docs (C. Barrandon, F. Fiorini, Z. Wang, E. Marquenet, O.S. Kwon) 7 Engineers (F. Bonneau, V. Murigneux, L. Bastianelli, R. Servignat d’Aout, R. Hocq, Q.Alasseur, J. Paternina) 1 technician CNRS (I. Barbosa). 9 Master students Since 2009: Master in Genetics, UPMC (3h per year) Since 2009: ENS L3 Chimie (2h per year) Since 2009: ENS Master in Genetics, UPMC (3h per year) 2014: PhD program, Wellcome Trust Centre for Cell Biology, Edinburgh, UK (2014) 2014: PhD program, FMI, Basel, Switzerland (2014) 2012: PhD program, University Toulouse (2012) 2017: Co-organizer and teaching: International course Curie-ENS “Posttranscriptional gene regulation” (2017) • Reviewer for several journals including: Cell; Nature; Nature Structural & Molecular Biology; Genes & Development; EMBO Journal; Journal of Cell Biology; Nucleic Acids Research; P.N.A.S.; Cell Report. • Reviewer for several grant agencies including: ERC; Wellcome Trust; ANR; Ligue contre le Cancer; Foreign agencies (Denmark, Poland, Israel, etc…). • Since 2013: Member executive council doctoral school “Complexité du Vivant” – University UPMC (Paris 6). • Since 2015: Member scientifc council “Ligue Grand Est” • 2013-2016: Member scientifc council “Fondation Pierre-Gilles de Gennes” • 2014: Vice-President ANR committee CES10 • 2016: Member ANR committee CES12 • 2017-2020: President ANR committee CES12 SELECTED PUBLICATIONS Kanaan Hocq R, Paternina J, Alasseur Q, Genovesio A, Le Hir H. (2018) Monitored eCLIP: high accuracy mapping of RNA-protein interactions. Nucleic Acids Research 2018 Nov;46(21):11553-11565. Kanaan J, Raj S, Decourty L, Saveanu C, Croquette V, Le Hir H. (2018) UPF1-like helicase grip on nucleic acids dictates processivity. Nature Communications. 2018 Sep;14(9):3752. Le Hir H, Saulière J, Wang Z. (2016) The exon junction complex as a node of postranscriptional networks. Nature Review in Molecular and Cellular Biology. 2016 Jan;17(1):41-54. Fiorini F, Bagchi D, Le Hir H*, Croquette V*. (2015) Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities. Nature Communications. Jul 3;6:7581. *Corresponding authors Wang Z, Murigneux V, Le Hir H. (2014). Transcriptome-wide modulation of splicing by the Exon Junction Complex. Genome Biology. 15(12):551. Chazal PE, Daguenet E, Wendling C, Ulrycke N, Tomasetto C, Sargueil B, Le Hir H (2013). EJC core component MLN51 interacts with eIF3 and activates translation. P.N.A.S. 110(15):5903-8. Saulière J, Murigneux V, Wang Z, Marquenet E, Barbosa I, Le Tonquèze O, Audic Y, Paillard L, Roest Crollius H, Le Hir H. (2012). CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex. Nature Structural and Molecular Biology 19(11):1124-31. Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, Le Hir H. (2012). Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly. Nature Structural and Molecular Biology 19(10):983-90. Chakrabarti S, Jayachandran U, Bonneau F, Fiorini F, Basquin C, Domcke S, Le Hir H, Conti E. (2011). Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and Its Regulation by Upf2. Molecular Cell 41(6):682-692. Saulière J, Haque N, Harms S, Barbosa I, Blanchette M, Le Hir H. (2010). The exon junction complex differentially marks spliced junctions. Nature Structural and Molecular Biology 17(10):1269-71. Le Hir H, Séraphin B. (2008). EJCs at the heart of translational control. Cell. 133(2):213-6. Chamieh H, Ballut L, Bonneau F, Le Hir H. (2008). NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity. Nature Structural and Molecular Biology 15(1):85-93. Andersen CB*, Ballut L*, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H#, Andersen GR#. (2006). Structure of the Exon Junction Core Complex with a Trapped DEAD-Box ATPase Bound to RNA. Science 313:1968-1972. Ballut L, Marchadier B, Baguet A, Tomasetto C, Séraphin B, Le Hir H. (2005). The Exon-Junction complex is locked onto mRNA by inhibition of eIF4A3 ATPase activity. Nature Structural and Molecular Biology 12(10):861-9. Le Hir, H., Gatfield, D., Izaurralde, E. & Moore, M.J. (2001). The exon-exon junction complex provides a binding platform for factors involved in mRNA export and NMD. The EMBO Journal 20 (17):4987-4997. Le Hir, H., Izaurralde, E., Maquat, L.E. & Moore, M.J. (2000). The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. The EMBO Journal 19 (24):6860-6869.Supervision activities
COMMUNICATIONS
Teaching activities
Peer review and expertise activity
Publications
Publications
Single molecule imaging reveals a conserved family of centrosomal mRNAs and a novel translation-dependent localization mechanism2022 ASCB Annual Meeting - Cell Bio Virtual 2022, Dec 2022, Washington (DC), United States. , Molecular Biology of the Cell, 34 (2), pp.B298/P1290, 2023 |
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Novel approaches to study helicases using magnetic tweezersHelicase Enzymes Part B, Elsevier, pp.359-403, 2022, Methods in Enzymology, ⟨10.1016/bs.mie.2022.03.035⟩ |
Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-Mediated mRNA DecayRNA helicases, pp.255-274, 2012, ⟨10.1016/B978-0-12-396546-2.00012-7⟩ |
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Biochemical insights into the conserved interactions of NMD factors from budding yeast to humans2026 |
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Chemical inhibition of Exon Junction Complex assembly impairs mRNA localization and neural stem cells ciliogenesis2025 |
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A localization screen reveals translation factories and widespread co-translational RNA targeting2020 |