Hervé LE HIR
28
Documents
Presentation
**CURRENT POSITION Research Director, 1st class - CNRS**
**Head of IBENS section "*Functional Genomics*"**
**Group leader, team "*Expression of eukaryotic mRNAs*"**
Institut de Biologie de l’Ecole Normale Supérieure (IBENS)–
UMR8197-U1024. 46 rue d’Ulm, 75005, Paris
**EDUCATION** 2006 : **HDR,** University Paris-Sud Orsay (Paris 11)
1998: **PhD, Molecular Biology,** University Paris Diderot (Paris 7)
1993 : **Master (B.A.) Biochemistry,** UPMC (Paris 6)
**DISTINCTION** 2009 :**ATIP** and **ATIP+** CNRS
2010, 2014, 2019 : **P**rime d’**E**ncadrement **D**octoral et de **R**echerche CNRS
**RESEARCH EXPERIENCE**
**Since 2009**: Group leader of the team "*Expression of eukaryotic mRNAs*"
IBENS – Section 2, Functional Genomics – Paris
**2001-2008**: CR2/1 CNRS in the group of Dr. Bertrand Séraphin
CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France.
**1998-2001**: Post-doctoral fellow in the lgroups of Dr. Lynne Maquat (Roswell Park Cancer Institute / Buffalo USA) and of Pr. Melissa J. Moore (HHMI / Brandeis University, MA. USA)
**1993-1998**: Ph.D. (PI: Dr. Claude Thermes) – University Paris 7
CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France.
**FUNDING**
**2018-2021**: **ANR blanche** – spEJCificity (Project leader)
**2015-2018**: **ANR blanche** – CleaNMD (Partner, coordinator Alain Jacquier)
**2014-2017**: **ANR blanche** – DifferEJnCe (Project leader)
**2014-2015**: **PSL research** – (Partner, coordinator Stephan Vagner)
**2014-2015**: **Association Recherche contre le Cancer** – (Project leader)
**2012-2015**: **FP7 Marie Curie Initial Training Network** « RNPnet »
**2012-2013**: **Fondation P.G. de Gennes** (Partner)
**2011-2013**: **ANR blanche** – EJCbirth (Project leader)
**2010-2013**: **Equipe FRM** (Project leader)
**2010:** **Installation Nouvelle Equipe FRM**
**2010-2011**: **Ligue Nationale Contre le Cancer** (Project leader)
**2009-2011**: **ANR blanche** – Explore EJC (Project leader)
**2009-2011**: **ATIP** CNRS
**2005-2008**: **ANR blanche** – L’EJC (Project leader)
**2003-2006**: **A.C.I. Jeunes Chercheurs**
**2003-2004**: **France-Berkeley Fund**
**Supervision activities**
==========================
1 Researcher (CR INSERM, J. Saulière)
6 PhD students (B. Marchadier, PE Chazal, R. Mishra, J. Kanaan, V. Busetto, J. Paternina)
5 Post-docs (C. Barrandon, F. Fiorini, Z. Wang, E. Marquenet, O.S. Kwon)
7 Engineers (F. Bonneau, V. Murigneux, L. Bastianelli, R. Servignat d’Aout, R. Hocq, Q.Alasseur, J. Paternina) 1 technician CNRS (I. Barbosa).
9 Master students
**COMMUNICATIONS**
==================
**Teaching activities**
=======================
Since 2009: Master in Genetics, UPMC (3h per year)
Since 2009: ENS L3 Chimie (2h per year)
Since 2009: ENS Master in Genetics, UPMC (3h per year)
2014: PhD program, Wellcome Trust Centre for Cell Biology, Edinburgh, UK (2014)
2014: PhD program, FMI, Basel, Switzerland (2014)
2012: PhD program, University Toulouse (2012)
2017: Co-organizer and teaching: International course Curie-ENS “Posttranscriptional
gene regulation” (2017)
**Peer review and expertise activity**
======================================
• Reviewer for several journals including: Cell; Nature; Nature Structural & Molecular Biology; Genes & Development; EMBO Journal; Journal of Cell Biology; Nucleic Acids Research; P.N.A.S.; Cell Report.
• Reviewer for several grant agencies including: ERC; Wellcome Trust; ANR; Ligue
contre le Cancer; Foreign agencies (Denmark, Poland, Israel, etc…).
• Since 2013: Member executive council doctoral school “Complexité du Vivant” – University UPMC (Paris 6).
• Since 2015: Member scientifc council “Ligue Grand Est”
• 2013-2016: Member scientifc council “Fondation Pierre-Gilles de Gennes”
• 2014: Vice-President ANR committee CES10
• 2016: Member ANR committee CES12 • 2017-2020: President ANR committee CES12
**SELECTED PUBLICATIONS**
Kanaan Hocq R, Paternina J, Alasseur Q, Genovesio A, **Le Hir H**. (**2018**) Monitored eCLIP: high accuracy mapping of RNA-protein interactions. **Nucleic Acids Research** 2018 Nov;46(21):11553-11565.
Kanaan J, Raj S, Decourty L, Saveanu C, Croquette V, **Le Hir H**. (**2018**) UPF1-like helicase grip on nucleic acids dictates processivity. **Nature Communications**. 2018 Sep;14(9):3752.
**Le Hir H**, Saulière J, Wang Z. (**2016**) The exon junction complex as a node of postranscriptional networks. **Nature Review in Molecular and Cellular Biology**. 2016 Jan;17(1):41-54.
Fiorini F, Bagchi D, **Le Hir H\***, Croquette V\*. (**2015**) Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities. **Nature Communications**. Jul 3;6:7581. \**Corresponding authors*
Wang Z, Murigneux V, **Le Hir H**. (**2014**). Transcriptome-wide modulation of splicing by the Exon Junction Complex. **Genome Biology**. 15(12):551.
Chazal PE, Daguenet E, Wendling C, Ulrycke N, Tomasetto C, Sargueil B, **Le Hir H** (**2013**). EJC core component MLN51 interacts with eIF3 and activates translation. **P.N.A.S.** 110(15):5903-8.
Saulière J, Murigneux V, Wang Z, Marquenet E, Barbosa I, Le Tonquèze O, Audic Y, Paillard L, Roest Crollius H, **Le Hir H**. (**2012**). CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex. **Nature Structural and Molecular Biology** 19(11):1124-31.
Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, **Le Hir H**. (**2012**). Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly. **Nature Structural and Molecular Biology** 19(10):983-90.
Chakrabarti S, Jayachandran U, Bonneau F, Fiorini F, Basquin C, Domcke S, Le Hir H, Conti E. (**2011**). Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and Its Regulation by Upf2. **Molecular Cell** 41(6):682-692.
Saulière J, Haque N, Harms S, Barbosa I, Blanchette M, Le Hir H. (**2010**). The exon junction complex differentially marks spliced junctions. **Nature Structural and Molecular Biology** 17(10):1269-71.
Le Hir H, Séraphin B. (**2008**). EJCs at the heart of translational control. **Cell**. 133(2):213-6.
Chamieh H, Ballut L, Bonneau F, Le Hir H. (**2008**). NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity. **Nature Structural and Molecular Biology** 15(1):85-93.
Andersen CB\*, Ballut L\*, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H#, Andersen GR#. (**2006**). Structure of the Exon Junction Core Complex with a Trapped DEAD-Box ATPase Bound to RNA. **Science** 313:1968-1972.
Ballut L, Marchadier B, Baguet A, Tomasetto C, Séraphin B, Le Hir H. (2005). The Exon-Junction complex is locked onto mRNA by inhibition of eIF4A3 ATPase activity. Nature Structural and Molecular Biology 12(10):861-9.
Le Hir, H., Gatfield, D., Izaurralde, E. & Moore, M.J. (**2001**). The exon-exon junction complex provides a binding platform for factors involved in mRNA export and NMD. **The EMBO Journal** 20 (17):4987-4997.
Le Hir, H., Izaurralde, E., Maquat, L.E. & Moore, M.J. (**2000**). The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. **The EMBO Journal** 19 (24):6860-6869.
Publications
|
Novel approaches to study helicases using magnetic tweezersHelicase Enzymes Part B, Elsevier, pp.359-403, 2022, Methods in Enzymology, ⟨10.1016/bs.mie.2022.03.035⟩
Book sections
hal-03727451v1
|
Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-Mediated mRNA DecayRNA helicases, pp.255-274, 2012, ⟨10.1016/B978-0-12-396546-2.00012-7⟩
Book sections
hal-02358966v1
|
|
A localization screen reveals translation factories and widespread co-translational RNA targeting2020
Preprints, Working Papers, ...
pasteur-02873144v1
|