Virgile Adam

Identifiants chercheurs

IdHAL :

virgile-adam

ORCID :

0000-0003-2209-7846

IdRef :

250406187

ResearcherID :

B-9359-2009

Publications

Article dans une revue (41)

The in crystallo optical spectroscopy toolbox Nicolas Caramello,Virgile Adam,Arwen Pearson,Antoine Royant Journal of Applied Crystallography, 2025, 58 (3), pp.1068-1078. ⟨10.1107/S1600576725003541⟩ Article dans une revue hal-05168361v1
Decoding mEos4b day-long maturation and engineering fast maturing variants Arijit Maity,Oleksandr Glushonkov,Isabel Ayala,Pascale Tacnet,Jip Wulffeléet al. Protein Science, 2025, 34 (8), pp.e70234. ⟨10.1002/pro.70234⟩ Article dans une revue hal-04743092v1
Photophysical Studies at Cryogenic Temperature Reveal a Novel Photoswitching Mechanism of rsEGFP2 Angela Mantovanelli,Oleksandr Glushonkov,Virgile Adam,Jip Wulffelé,Daniel Thédiéet al. Journal of the American Chemical Society, 2023, 145 (27), pp.14636-14646. ⟨10.1021/jacs.3c01500⟩ Article dans une revue hal-04162903v1
Structural heterogeneity in a phototransformable fluorescent protein impacts its photochemical properties Arijit Maity,Jip Wulffelé,Isabel Ayala,Adrien Favier,Virgile Adamet al. Advanced Science, 2023, pp.e2306272. ⟨10.1002/advs.202306272⟩ Article dans une revue hal-04426227v1
Rational control of off‐state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement Virgile Adam,Kyprianos Hadjidemetriou,Nickels Jensen,Robert L. Shoeman,Joyce Woodhouseet al. ChemPhysChem, 2022, 23 (19), pp.e202200192. ⟨10.1002/cphc.202200192⟩ Article dans une revue hal-03758975v1
Supramolecular assembly of the Escherichia coli LdcI upon acid stress Matthew Jessop,Clarissa Liesche,Jan Felix,Ambroise Desfosses,Megghane Baulardet al. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (2), pp.e2014383118. ⟨10.1073/pnas.2014383118⟩ Article dans une revue hal-03112998v1
Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy Nina Eleni Christou,Karine Giandoreggio-Barranco,Isabel Ayala,Oleksandr Glushonkov,Virgile Adamet al. Journal of the American Chemical Society, 2021, 143 (19), pp.7521-7530. ⟨10.1021/jacs.1c02442⟩ Article dans une revue hal-03328569v1
Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State Elke de Zitter,Jacqueline Ridard,Daniel Thédié,Virgile Adam,Bernard C. Levyet al. Journal of the American Chemical Society, 2020, 142 (25), pp.10978-10988. ⟨10.1021/jacs.0c01880⟩ Article dans une revue hal-02895781v1
Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy. Joyce Woodhouse,Gabriela Nass Kovacs,Nicolas Coquelle,Lucas M. Uriarte,Virgile Adamet al. Nature Communications, 2020, 11 (1), pp.741. ⟨10.1038/s41467-020-14537-0⟩ Article dans une revue hal-02498645v1
NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein Nina Eleni Christou,Isabel Ayala,Karine Giandoreggio-Barranco,Martin Byrdin,Virgile Adamet al. Biophysical Journal, 2019, 117 (11), pp.2087-2100. ⟨10.1016/j.bpj.2019.10.035⟩ Article dans une revue hal-02389522v1
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM Elke de Zitter,Daniel Thédié,Viola Mönkemöller,Siewert Hugelier,Joël Beaudouinet al. Nature Methods, 2019, 16 (8), pp.707-710. ⟨10.1038/s41592-019-0462-3⟩ Article dans une revue hal-02270788v1
Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography Nicolas Coquelle,Michel Sliwa,Joyce Woodhouse,Giorgio Schirò,Virgile Adamet al. Nature Chemistry, 2018, 10 (1), pp.31-37. ⟨10.1038/nchem.2853⟩ Article dans une revue hal-01618533v1
Photoswitching of Green mEos2 by Intense 561 nm Light Perturbs Efficient Green-to-Red Photoconversion in Localization Microscopy Daniel Thédié,Romain Berardozzi,Virgile Adam,Dominique Bourgeois Journal of Physical Chemistry Letters, 2017, 8 (18), pp.4424 - 4430. ⟨10.1021/acs.jpclett.7b01701⟩ Article dans une revue hal-01592521v1
Arginine 66 Controls Dark-State Formation in Green-to-Red Photoconvertible Fluorescent Proteins Romain Berardozzi,Virgile Adam,Alexandre Martins,Dominique Bourgeois Journal of the American Chemical Society, 2016, 138 (2), pp.558-565. ⟨10.1021/jacs.5b09923⟩ Article dans une revue hal-01266594v1
Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm Mariam El Khatib,Alexandre Martins,Dominique Bourgeois,Jacques-Philippe Colletier,Virgile Adam Scientific Reports, 2016, 6, ⟨10.1038/srep18459⟩ Article dans une revue hal-01280266v1
Serial femtosecond crystallography and ultrafast absorption spectroscopy of the photoswitchable fluorescent protein IrisFP Jacques-Philippe Colletier,M. Sliwa,François-Xavier Gallat,M. Sugahara,Virginia Guillonet al. Journal of Physical Chemistry Letters, 2016, 7 (5), pp.882 - 887. ⟨10.1021/acs.jpclett.5b02789⟩ Article dans une revue hal-01285611v1
Remodeling of the Z-Ring Nanostructure during the Streptococcus pneumoniae Cell Cycle Revealed by Photoactivated Localization Microscopy. Maxime Jacq,Virgile Adam,Dominique Bourgeois,Christine Moriscot,Anne-Marie Di Guilmiet al. mBio, 2015, 6 (4), pp.e01108-15 Article dans une revue hal-01199165v1
Rational design of enhanced photoresistance in a photoswitchable fluorescent protein Chenxi Duan,Martin Byrdin,Mariam El Kathib,Xavier Henry,Virgile Adamet al. Methods and Applications in Fluorescence, 2015, 3 (1), pp.XXX. ⟨10.1088/2050-6120/3/1/014004⟩ Article dans une revue hal-01161782v1
La microscopie de fluorescence à super-résolution : un outil révolutionnaire pour imager le vivant à l'échelle nanométrique. Virgile Adam,Dominique Bourgeois Photoniques, 2015, 74, pp.34-39 Article dans une revue hal-01158907v1
Phototransformable fluorescent proteins: Future challenges. Virgile Adam,Romain Berardozzi,Martin Byrdin,Dominique Bourgeois Current Opinion in Chemical Biology, 2014, 20, pp.92-102 Article dans une revue hal-01093360v1
In cellulo Evaluation of Phototransformation Quantum Yields in Fluorescent Proteins Used As Markers for Single-Molecule Localization Microscopy Sergiy Avilov,Romain Berardozzi,Mudalige S. Gunewardene,Virgile Adam,Samuel T. Hesset al. PLoS ONE, 2014, 9 (6), pp.Volume 9 | Issue 6 | e98362. ⟨10.1371/journal.pone.0098362⟩ Article dans une revue hal-01093348v1
Excited state dynamics of the photoconvertible fluorescent protein Kaede revealed by ultrafast spectroscopy E. Fron,M. Sliwa,Virgile Adam,J. Michiels,Susana Rochaet al. Photochemical & Photobiological Sciences , 2014, 13, pp.867 - 874. ⟨10.1039/c3pp50335f⟩ Article dans une revue hal-01113460v1
Phototransformable fluorescent proteins: which one for which application? Virgile Adam Histochemistry and Cell Biology, 2014, 142 (1), pp.19-41 Article dans une revue hal-01131208v1
Structural basis of photoswitching in fluorescent proteins. Chenxi Duan,Virgile Adam,Martin Byrdin,Dominique Bourgeois Methods in Molecular Biology, 2014, 1148, pp.177-202 Article dans une revue hal-01131245v1
Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2 Eduard Fron,Mark van Der Auweraer,Benjamien Moeyaert,Jan Michiels,Hideaki Mizunoet al. Journal of Physical Chemistry B, 2013, 117 (8), pp.2300 - 2313. ⟨10.1021/jp309219m⟩ Article dans une revue hal-01662511v1
Structural evidence for a two-regime photobleaching mechanism in a reversibly switchable fluorescent protein. Chenxi Duan,Virgile Adam,Martin Byrdin,Jacqueline Ridard,Sylvie Kieffer-Jaquinodet al. Journal of the American Chemical Society, 2013, 135 (42), pp.15841-50 Article dans une revue hal-01322350v1
Structural Evidence for a Two-Regime Photobleaching Mechanism in a Reversibly Switchable Fluorescent Protein Chenxi Duan,Virgile Adam,Martin Byrdin,Jacqueline Ridard,Sylvie Kieffer-Jaquinodet al. Journal of the American Chemical Society, 2013, 135 (42), pp.15841 - 15850. ⟨10.1021/ja406860e⟩ Article dans une revue hal-02650196v1
Photoactivated structural dynamics of fluorescent proteins Dominique Bourgeois,Aline Regis-Faro,Virgile Adam Biochemical Society Transactions, 2012, 40 (3), pp.531-538. ⟨10.1042/BST20120002⟩ Article dans une revue hal-02389719v1
Reversible photoswitching in fluorescent proteins: A mechanistic view Dominique Bourgeois,Virgile Adam IUBMB Life, 2012, 64 (6), pp.482-491. ⟨10.1002/iub.1023⟩ Article dans une revue hal-02389714v1
From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family Jörg Wiedenmann,Susan Gayda,Virgile Adam,Franz Oswald,Karin Nienhauset al. Journal of Biophotonics, 2011, 4 (6), pp.377-390. ⟨10.1002/jbio.201000122⟩ Article dans une revue istex hal-02389707v1
Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications Virgile Adam,Benjamien Moeyaert,Charlotte c. David,Hideaki Mizuno,Mickaël Lelimousinet al. Chemistry and Biology, 2011, 18 (10), pp.1241-1251. ⟨10.1016/j.chembiol.2011.08.007⟩ Article dans une revue hal-02389699v1
The Nature of Transient Dark States in a Photoactivatable Fluorescent Protein Arijit Roy,Martin J Field,Virgile Adam,Dominique Bourgeois Journal of the American Chemical Society, 2011, 133 (46), pp.18586-18589. ⟨10.1021/ja2085355⟩ Article dans une revue hal-02389684v1
Low-temperature switching by photoinduced protonation in photochromic fluorescent proteins Aline Regis Faro,Virgile Adam,Philippe Carpentier,Claudine Darnault,Dominique Bourgeoiset al. Photochemical & Photobiological Sciences , 2010, 9 (2), pp.254. ⟨10.1039/b9pp00121b⟩ Article dans une revue istex hal-02389665v1
Fine Tuning the Optical Properties of Green to Red Photoconvertible Fluorescent Proteins Karin Nienhaus,Virgile Adam,Dominique Bourgeois,G. Ulrich Nienhaus Biophysical Journal, 2010, 98 (3), pp.413a. ⟨10.1016/j.bpj.2009.12.2228⟩ Article dans une revue hal-02389667v1
Data storage based on photochromic and photoconvertible fluorescent proteins Virgile Adam,Hideaki Mizuno,Alexei Grichine,Jun-Ichi Hotta,Yutaka Yamagataet al. Journal of Biotechnology, 2010, 149 (4), pp.289-298. ⟨10.1016/j.jbiotec.2010.04.001⟩ Article dans une revue istex hal-02389676v1
Photoconversion of the Fluorescent Protein EosFP: A Hybrid Potential Simulation Study Reveals Intersystem Crossings Mickaël Lelimousin,Virgile Adam,G. Ulrich Nienhaus,Dominique Bourgeois,Martin J Field Journal of the American Chemical Society, 2009, 131 (46), pp.16814-16823. ⟨10.1021/ja905380y⟩ Article dans une revue hal-02389649v1
Structural basis of X-ray-induced transient photobleaching in a photoactivatable green fluorescent protein. Virgile Adam,Philippe Carpentier,Sebastien Violot,Mickaël Lelimousin,Claudine Darnaultet al. Journal of the American Chemical Society, 2009, 131 (50), pp.18063-5. ⟨10.1021/ja907296v⟩ Article dans une revue hal-00474158v1
Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme. Gergely Katona,Philippe Carpentier,Vincent Nivière,Patricia Amara,Virgile Adamet al. Science, 2007, pp.449-53 Article dans une revue hal-01075775v1
Detoxification of superoxide without production of H2O2: antioxidant activity of superoxide reductase complexed with ferrocyanide. Fernando P Molina-Heredia,Chantal Houée-Levin,C. Berthomieu,Danièle Touati,Emilie Tremeyet al. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103 (40), pp.14750-5. ⟨10.1073/pnas.0510828103⟩ Article dans une revue hal-01075778v1
The crystal structure of Mycobacterium tuberculosis thymidylate kinase in complex with 3'-azidodeoxythymidine monophosphate suggests a mechanism for competitive inhibition. Emanuela Fioravanti,Virgile Adam,Hélène Munier-Lehmann,Dominique Bourgeois Biochemistry, 2005, 44 (1), pp.130-7. ⟨10.1021/bi0484163⟩ Article dans une revue istex pasteur-00166954v1
Structure of superoxide reductase bound to ferrocyanide and active site expansion upon X-ray-induced photo-reduction. Virgile Adam,Antoine Royant,Vincent Nivière,Fernando P Molina-Heredia,Dominique Bourgeois Structure, 2004, 12, pp.1729-40. ⟨10.1016/j.str.2004.07.013⟩ Article dans une revue hal-01075786v1

Pré-publication, Document de travail (1)

Photophysical studies at cryogenic temperature reveal a novel photoswitching mechanism of rsEGFP2 Angela Mantovanelli,Oleksandr Glushonkov,Virgile Adam,Jip Wulffele,Daniel Thédiéet al. 2022 Pré-publication, Document de travail hal-03810073v1

Thèse (1)

Mechanistic studies of photoactivatable fluorescent proteins: a combined approach by crystallography and spectroscopy Virgile Adam Biological Physics [physics.bio-ph]. Université Joseph-Fourier - Grenoble I, 2009. English. ⟨NNT : ⟩ Thèse tel-00449332v1

HDR (1)

Etudes photo-physiques et ingénierie des protéines fluorescentes photo-transformables Virgile Adam Biophysique. Université Grenoble Alpes, 2023 HDR tel-04322660v1