Yong Chen

71
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Publications

Publications

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Piezoelectric and microfluidic tuning of an infrared cavity for vibrational polariton studies

Wei Wang , Jaime de la Fuente Diez , Nicolas Delsuc , Juan Peng , Riccardo Spezia et al.
Lab on a Chip, 2024, 24 (9), pp.2497-2505. ⟨10.1039/D3LC01101A⟩
Article dans une revue hal-04572532 v1
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Diffusion-based culture and real-time impedance monitoring of tumor spheroids in hydrogel microwells of a suspended membrane under microfluidic conditions

Wei Wang , Yuanhui Liu , Xiaochen Huang , Feng Liang , Haoyue Luo et al.
Talanta, 2024, 278, pp.126473. ⟨10.1016/j.talanta.2024.126473⟩
Article dans une revue hal-04635076 v1
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High-porosity thin membrane for high-efficiency capture of rare cells

Haoyue Luo , Feng Liang , Wei Wang , Xiaochen Huang , Zheng Mao et al.
Sensors and Actuators B: Chemical, 2024, 398, pp.134720. ⟨10.1016/j.snb.2023.134720⟩
Article dans une revue hal-04242676 v1
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Aerosol Jet-Printed High-Aspect Ratio Micro-Needle Electrode Arrays Applied for Human Cerebral Organoids and 3D Neurospheroid Networks

Sabine Zips , Boxin Huang , Salammbô Hotte , Lukas Hiendlmeier , Chen Wang et al.
ACS Applied Materials & Interfaces, 2023, ⟨10.1021/acsami.3c06210⟩
Article dans une revue hal-04167370 v1
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Aligned Nanofibrous Net Deposited Perpendicularly on Microridges Supports Endothelium Formation and Promotes the Structural Maturation of hiPSC-Derived Cardiomyocytes

Feng Tian , Linlin Yin , Peiran Lin , Yurong Liu , Wenlong Wang et al.
ACS Applied Materials & Interfaces, 2023, 15 (14), pp.17518-17531. ⟨10.1021/acsami.2c22551⟩
Article dans une revue hal-04240029 v1
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On-chip real-time impedance monitoring of hiPSC-derived and artificial basement membrane-supported endothelium

Xiaochen Huang , Feng Liang , Boxin Huang , Haoyue Luo , Jian Shi et al.
Biosensors and Bioelectronics, 2023, 235, ⟨10.1016/j.bios.2023.115324⟩
Article dans une revue hal-04239989 v1
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A microfluidic tool for real-time impedance monitoring of in vitro renal tubular epithelial cell barrier

Feng Liang , Xiaochen Huang , Boxin Huang , Yong He , Haoyue Luo et al.
Sensors and Actuators B: Chemical, 2023, 392, pp.134077. ⟨10.1016/j.snb.2023.134077⟩
Article dans une revue hal-04240032 v1

Plant-on-chip: Core morphogenesis processes in the tiny plant Wolffia australiana

Feng Li , Jing-Jing Yang , Zong-Yi Sun , Lei Wang , Le-Yao Qi et al.
PNAS Nexus, 2023, 2 (5), ⟨10.1093/pnasnexus/pgad141⟩
Article dans une revue hal-04239991 v1
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High-throughput tuning of ovarian cancer spheroids for on-chip invasion assays

Changchong Chen , Yong He , Elliot Lopez , Franck Carreiras , Ayako Yamada et al.
Micro and Nano Engineering, 2022, 15, pp.100138. ⟨10.1016/j.mne.2022.100138⟩
Article dans une revue hal-03684974 v1
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Reconstituted basement membrane enables airway epithelium modeling and nanoparticle toxicity testing

Elrade Rofaani , Boxin Huang , Feng Liang , Juan Peng , Yong Chen
International Journal of Biological Macromolecules, 2022, 204, pp.300-309. ⟨10.1016/j.ijbiomac.2022.02.018⟩
Article dans une revue hal-03818520 v1
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Sars‐Cov‐2 Spike Protein‐Induced Damage of hiPSC‐Derived Cardiomyocytes

Xiaochen Huang , Boxin Huang , Yong He , Liang Feng , Jian Shi et al.
Advanced Biology, In press, ⟨10.1002/adbi.202101327⟩
Article dans une revue hal-03664385 v1
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Generation of Alveolar Epithelium Using Reconstituted Basement Membrane and hiPSC‐Derived Organoids

Yong He , Elrade Rofaani , Xiaochen Huang , Boxin Huang , Feng Liang et al.
Advanced Healthcare Materials, 2022, 11 (6), pp.2101972. ⟨10.1002/adhm.202101972⟩
Article dans une revue hal-03818521 v1
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Modulation of designer biomimetic matrices for optimized differentiated intestinal epithelial cultures

Wang Xi , Jad Saleh , Ayako Yamada , Caterina Tomba , Barbara Mercier et al.
Biomaterials, 2022, 282, pp.121380. ⟨10.1016/j.biomaterials.2022.121380⟩
Article dans une revue hal-03598543 v1
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Automatic differentiation of human induced pluripotent stem cells toward synchronous neural networks on an arrayed monolayer of nanofiber membrane

Boxin Huang , Yong He , Elrade Rofaani , Feng Liang , Xiaochen Huang et al.
Acta Biomaterialia, 2022, 150, pp.168-180. ⟨10.1016/j.actbio.2022.07.038⟩
Article dans une revue hal-03818522 v1
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Epithelial folding of alveolar cells derived from human induced pluripotent stem cells on artificial basement membrane

Elrade Rofaani , Yong He , Juan Peng , Yong Chen
Acta Biomaterialia, In press, ⟨10.1016/j.actbio.2022.03.022⟩
Article dans une revue hal-03818519 v1
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Nanocasting of fibrous morphology on a substrate for long-term propagation of human induced pluripotent stem cells

Sisi Li , Momoko Yoshioka , Junjun Li , Li Liu , Shixin Ye et al.
Biomedical Materials, 2022, 17 (2), pp.025014. ⟨10.1088/1748-605X/ac51b8⟩
Article dans une revue hal-03601217 v1
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Three-Dimensional Collagen Topology Shapes Cell Morphology, beyond Stiffness

Changchong Chen , Zeinab Ibrahim , Marion Marchand , Tristan Piolot , Sahil Kamboj et al.
ACS Biomaterials Science and Engineering, 2022, 8 (12), pp.5284-5294. ⟨10.1021/acsbiomaterials.2c00879⟩
Article dans une revue hal-03926139 v1
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Microfluidic release of the rare cells captured by a filter with tapered holes

Feng Liang , Juan Peng , Jian Shi , Li Wang , Carole Aimé et al.
Micro and Nano Engineering, 2022, 14, pp.100119. ⟨10.1016/j.mne.2022.100119⟩
Article dans une revue hal-03687625 v1
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Generation of Interconnected Neural Clusters in Multiscale Scaffolds from Human-Induced Pluripotent Stem Cells

Boxin Huang , Juan Peng , Xiaochen Huang , Feng Liang , Li Wang et al.
ACS Applied Materials & Interfaces, 2021, 13 (47), pp.55939-55952. ⟨10.1021/acsami.1c18465⟩
Article dans une revue hal-03442115 v1
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Highly sensitive nonenzymetic glucose sensing based on multicomponent hierarchical NiCo-LDH/CCCH/CuF nanostructures

Zhenting Zhao , Yongjiao Sun , Jinxiang Song , Yajia Li , Yun Xie et al.
Sensors and Actuators B: Chemical, 2021, 326, pp.128811 -. ⟨10.1016/j.snb.2020.128811⟩
Article dans une revue hal-03491975 v1

Highly sensitive and portable electrochemical detection system based on AuNPs@CuO NWs/Cu2O/CF hierarchical nanostructures for enzymeless glucose sensing

Zhenting Zhao , Qiuguo Li , Yongjiao Sun , Cancan Zhao , Zhaohui Guo et al.
Sensors and Actuators B: Chemical, 2021, 345, pp.130379. ⟨10.1016/j.snb.2021.130379⟩
Article dans une revue hal-03442094 v1
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Native Collagen: Electrospinning of Pure, Cross-Linker-Free, Self-Supported Membrane

Dounia Dems , Julien Rodrigues da Silva , Christophe Hélary , Frank Wien , Marion Marchand et al.
ACS Applied Bio Materials, 2020, ⟨10.1021/acsabm.0c00006⟩
Article dans une revue hal-02541707 v1
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Fabrication of ultrathin artificial basement membrane for epithelial cell culture

Elrade Rofaani , Juan Peng , Li Wang , Yong He , Boxin Huang et al.
Microelectronic Engineering, 2020, 232, pp.111407. ⟨10.1016/j.mee.2020.111407⟩
Article dans une revue hal-03013350 v1

Evaluating impacts of pulse fishing on the effectiveness of seasonal closure

Lei Xing , Yong Chen , Chongliang Zhang , Bai Li , Yunne-Jai Shin et al.
Acta Oceanologica Sinica, 2020, 39 (4), pp.89--99. ⟨10.1007/s13131-020-1536-x⟩
Article dans une revue hal-04934358 v1
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Alveolar mimics with periodic strain and its effect on the cell layer formation

Milad Radiom , Yong He , Juan Peng , Armelle Baeza-Squiban , Jean-François Berret et al.
Biotechnology and Bioengineering, 2020, 117 (9), pp.2827-2841. ⟨10.1002/bit.27458⟩
Article dans une revue hal-02868726 v1
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Fabrication of micro-cages and caged tumor spheroids for microfluidic chip- based assays

Yong He , Boxin Huang , Elrade Rofaani , Jie Hu , Yuanhui Liu et al.
Microelectronic Engineering, 2020, 225, pp.111256. ⟨10.1016/j.mee.2020.111256⟩
Article dans une revue hal-02565343 v2
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Microfluidic channel with embedded monolayer nanofibers for cell culture and co-culture

Boxin Huang , Yong He , Li Wang , Jian Shi , Jie Hu et al.
Microelectronic Engineering, 2020, 225, pp.111235. ⟨10.1016/j.mee.2020.111235⟩
Article dans une revue hal-02565348 v2
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An automatic cell culture platform for differentiation of human induced pluripotent stem cells

Gabriele Pitingolo , Yong He , Boxin Huang , Li Wang , Jian Shi et al.
Microelectronic Engineering, 2020, 231, pp.111371. ⟨10.1016/j.mee.2020.111371⟩
Article dans une revue hal-03013336 v1
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Engineering of Droplet Charges in Microfluidic Chips

Rongxiang He , Meilin Ruan , Yuyang Qi , Hongni Liu , Zhengtao Zhang et al.
Advanced Engineering Materials, 2020, 22 (4), pp.1901521. ⟨10.1002/adem.201901521⟩
Article dans une revue hal-03442107 v1
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Circulating re-entrant waves promote maturation of hiPSC-derived cardiomyocytes in self-organized tissue ring

Junjun Li , Lu Zhang , Leqian Yu , Itsunari Minami , Shigeru Miyagawa et al.
Communications Biology, 2020, 3 (1), ⟨10.1038/s42003-020-0853-0⟩
Article dans une revue hal-03001825 v1
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Circulating re-entrant waves promote maturation of hiPSC-derived cardiomyocytes in self-organized tissue ring

Junjun Li , Lu Zhang , Leqian Yu , Itsunari Minami , Shigeru Miyagawa et al.
Communications Biology, 2020, 3, pp.122. ⟨10.1038/s42003-020-0853-0⟩
Article dans une revue hal-02514949 v1
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Fabrication of elastomer pillar arrays with elasticity gradient for cell migration, elongation and patterning

Bin Wang , Jian Shi , Jin Wei , Xiaolong Tu , Yong Chen
Biofabrication, 2019, 11 (4), pp.045003. ⟨10.1088/1758-5090/ab21b3⟩
Article dans une revue hal-03016323 v1
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Effect of oblique polymer pillars on spreading and elongation of rat mesenchymal stem cells

Jie Hu , Yan-Jun Liu , Jian Shi , Li Wang , Matthieu Piel et al.
Colloids and Surfaces B: Biointerfaces, 2019, 183, pp.110485. ⟨10.1016/j.colsurfb.2019.110485⟩
Article dans une revue hal-02415221 v1
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Heart on a chip: Micro-nanofabrication and microfluidics steering the future of cardiac tissue engineering

Maria Kitsara , Dimitrios Kontziampasis , Onnik Agbulut , Yong Chen
Microelectronic Engineering, 2019, 203-204, pp.44-62. ⟨10.1016/j.mee.2018.11.001⟩
Article dans une revue hal-02415223 v1
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Electro-capture of heavy metal ions with carbon cloth integrated microfluidic devices

Francois-Marie Allioux , Pankaj Kapruwan , Nicholas Milne , Lingxue Kong , Jacques Fattaccioli et al.
Separation and Purification Technology, 2018, 194, pp.26-32. ⟨10.1016/j.seppur.2017.10.064⟩
Article dans une revue hal-04019160 v1
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A micro-/nano-chip and quantum dots-based 3D cytosensor for quantitative analysis of circulating tumor cells

Xuan Wu , Tingyu Xiao , Zhang Luo , Rongxiang He , Yiping Cao et al.
Journal of Nanobiotechnology, 2018, 16, pp.65. ⟨10.1186/s12951-018-0390-x⟩
Article dans une revue hal-01884713 v1
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Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

Maria Kitsara , Onnik Agbulut , Dimitrios Kontziampasis , Yong Chen , Philippe Menasché
Acta Biomaterialia, 2017, 48, pp.20-40. ⟨10.1016/j.actbio.2016.11.014⟩
Article dans une revue hal-01628334 v1
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Preparation and characterization of AuNPs/CNTs-ErGO electrochemical sensors for highly sensitive detection of hydrazine

Zhenting Zhao , Yongjiao Sun , Pengwei Li , Wendong Zhang , Kun Lian et al.
Talanta, 2016, 158, pp.283-291. ⟨10.1016/j.talanta.2016.05.065⟩
Article dans une revue hal-01324925 v1
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Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo

Marie-Aude Plamont , Emmanuelle Billon-Denis , Sylvie Maurin , Carole Gauron , Frederico M. Pimenta et al.
Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (3), pp.497-502. ⟨10.1073/pnas.1513094113⟩
Article dans une revue hal-01259909 v1

Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers †

Yadong Tang , Li Liu , Junjun Li , Leqian Yu , Francesco Paolo Ulloa Severino et al.
Journal of materials chemistry‎ B, 2016, ⟨10.1039/c6tb00351f⟩
Article dans une revue hal-01345448 v1
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Fabrication of adjacent micropillar arrays with different heights for cell studies

Jin Wei , Jian Shi , Bin Wang , Yadong Tang , Xiaolong Tu et al.
Microelectronic Engineering, 2016, ⟨10.1016/j.mee.2016.03.008⟩
Article dans une revue hal-01285505 v1
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Nanometric emulsions encapsulating solid particles as alternative carriers for intracellular delivery

Sandrine Quignard , Ghislaine Frebourg , Yong Chen , Jacques Fattaccioli
Nanomedicine, 2016, 11 (16), pp.2059-2072. ⟨10.2217/nnm-2016-0074⟩
Article dans une revue hal-01536652 v1
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3D printed PEGDA microstructures for gelatin scaffold integration and neuron differentiation

Xiaolong Tu , Lina Wang , Wei Jin , Bin Wang , Yadong Tang et al.
Microelectronic Engineering, 2016, ⟨10.1016/j.mee.2016.03.007⟩
Article dans une revue hal-01285501 v1

Luminescent and absorptive metal-coated emulsions for micro-velocimetry

Olivier Mesdjian , Yong Chen , Jacques Fattaccioli
Microelectronic Engineering, 2016, ⟨10.1016/j.mee.2016.03.028⟩
Article dans une revue hal-01290056 v1

Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections

Timothée Houssin , Jérémy Cramer , Rébecca Grojsman , Lyes Bellahsene , Guillaume Colas et al.
Lab Chip, 2016, in press. ⟨10.1039/C5LC01459J⟩
Article dans une revue hal-01295648 v1
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Agarose multi-wells for tumour spheroid formation and anti-cancer drug test

Yadong Tang , Jianmiao Liu , Yong Chen
Microelectronic Engineering, 2016, ⟨10.1016/j.mee.2016.03.009⟩
Article dans une revue hal-01285507 v1
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Fabrication of cardiac patch by using electrospun collagen fibers

Maria Kitsara , Pierre Joanne , Solène Emmanuelle Boitard , Ibtihel Ben Dhiab , Barbara Poinard et al.
Microelectronic Engineering, 2015, 144, pp.46-50. ⟨10.1016/j.mee.2015.02.034⟩
Article dans une revue hal-01130222 v1
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Phagocytosis of immunoglobulin-coated emulsion droplets

Kalthoum Ben M'Barek , Diana Molino , Sandrine Quignard , Marie-Aude Plamont , Yong Chen et al.
Biomaterials, 2015, 51, pp.270-277. ⟨10.1016/j.biomaterials.2015.02.030⟩
Article dans une revue hal-01137459 v1
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Nanofibrous clinical-grade collagen scaffolds seeded with human cardiomyocytes induces cardiac remodeling in dilated cardiomyopathy

Pierre Joanne , Maria Kitsara , Solène-Emmanuelle Boitard , Hany Naemetalla , Valérie Vanneaux et al.
Biomaterials, 2015, 80, pp.157-168. ⟨10.1016/j.biomaterials.2015.11.035⟩
Article dans une revue hal-01250352 v1
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Microfluidic device with integrated microfilter of conical-shaped holes for high efficiency and high purity capture of circulating tumor cells

Yadong Tang , Jian Shi , Sisi Li , Li Wang , Yvon E. Cayre et al.
Scientific Reports, 2014, 4, pp.6052. ⟨10.1038/srep06052⟩
Article dans une revue hal-01358813 v1

In situ, real time monitoring of surface transformation : ellipsometric microscopy imaging of electrografting at microstructured gold surfaces

S. Munteanu , N. Garraud , J.P. Roger , Fabien Amiot , Jian Shi et al.
Analytical Chemistry, 2013, 85 (4), pp.1965-1971. ⟨10.1021/ac3034085⟩
Article dans une revue hal-00797107 v1

Patterning cells and shear flow conditions: convenient observation of endothelial cell remoulding, enhanced production of angiogenesis factors and drug response.

Li Wang , Zhi-Ling Zhang , Joanna Wdzieczak-Bakala , Dai-Wen Pang , Jianmiao Liu et al.
Lab on a Chip, 2011, 11 (24), pp.4235-40. ⟨10.1039/c1lc20722a⟩
Article dans une revue hal-00689145 v1

Fast microfluidic temperature control for high resolution live cell imaging

G. Velve-Casquillas , C. Fu , M. Le Berre , Jeremy Cramer , Sébastien Méance et al.
Lab on a Chip, 2011, 11, pp.484
Article dans une revue hal-00679141 v1

Fabrication of ZnO micro- and nano-structures by electrodeposition using nanoporous and lithography defined templates

Yamin Leprince-Wang , Salah Bouchaib , Tayeb Brouri , Martine Capo-Chichi , Kevin Laurent et al.
Materials Science and Engineering: B, 2010, 170, pp.107-112. ⟨10.1016/j.mseb.2010.02.037⟩
Article dans une revue istex hal-00504384 v1

Photomanipulation of a droplet by the chromocapillary effect

Antoine Diguet , Reine-Marie Guillermic , Nobuyuki Magome , Arnaud Saint-Jalmes , Yong Chen et al.
Angewandte Chemie International Edition, 2009, 48 (49), pp.9281-4. ⟨10.1002/anie.200904868⟩
Article dans une revue istex hal-00662830 v1

Degree of polarization of thermal light emitted by gratings supporting surface waves

François Marquier , Christophe Arnold , Marine Laroche , Jean-Jacques Greffet , Yong Chen
Optics Express, 2008, 16, pp.5305. ⟨10.1364/oe.16.005305⟩
Article dans une revue hal-00270145 v1

Fabrication of SOI photonic crystal slabs by soft UV-nanoimprint lithography

Michele Belotti , Jeremi Torres , Emmanuel Roy , Anne Pépin , Dario Gerace et al.
Microelectronic Engineering, 2006, 83 (4-9), pp.1773 - 1777. ⟨10.1016/j.mee.2005.12.025⟩
Article dans une revue istex hal-01801472 v1

Replication of photonic crystals by soft ultraviolet-nanoimprint lithography

Michele Belotti , Jeremi Torres , Emanuel Roy , Anne Pépin , Yong Chen et al.
Journal of Applied Physics, 2006, 99 (2), ⟨10.1063/1.2159544⟩
Article dans une revue hal-01801475 v1

Bouncing transitions on microtextured materials

Mathilde Reyssat , Anne Pépin , Frédéric Marty , Yong Chen , David Quéré
EPL - Europhysics Letters, 2006, 74 (2), pp.306-312. ⟨10.1209/epl/i2005-10523-2⟩
Article dans une revue hal-02386495 v1

Microfabricated textured surfaces for super-hydrophobicity investigations

Mathilde Callies , Yong Chen , Frédéric Marty , Anne Pépin , David Quéré
Microelectronic Engineering, 2005, 78-79, pp.100-105. ⟨10.1016/j.mee.2004.12.093⟩
Article dans une revue istex hal-02386375 v1

Coherent spontaneous light emission

Jean-Jacques Greffet , François Marquier , Remi Carminati , Karl Joulain , Jean-Philippe Mulet et al.
Journal de Physique IV Proceedings, 2004, 119, pp.35
Article dans une revue hal-00270124 v1
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Short Bragg mirrors with adiabatic modal conversion

David Peyrade , Eric Silberstein , Philippe Lalanne , Anne Talneau , Yong Chen
Applied Physics Letters, 2002, 81 (2), pp.829-831. ⟨10.1063/1.1497722⟩
Article dans une revue hal-00878129 v1

Uniaxial-stress investigation of asymmetrical GaAs-(Ga,Al)As double quantum wells.

Bernard Gil , Pierre Lefebvre , Bonnel Philippe , Henry Mathieu , Christiane Deparis et al.
Physical Review B: Condensed Matter and Materials Physics (1998-2015), 1993, 47 (4), pp.1954. ⟨10.1103/PhysRevB.47.1954⟩
Article dans une revue hal-01176102 v1

Reflectance study of inter-well couplings in GaAs-(Ga,Al)As double quantum wells.

Bonnel Philippe , Pierre Lefebvre , Bernard Gil , Henry Mathieu , Christiane Deparis et al.
Physical Review B: Condensed Matter and Materials Physics (1998-2015), 1990, 42 (6), pp.3435. ⟨10.1103/PhysRevB.42.3435⟩
Article dans une revue hal-01175677 v1

Exchange effects on excitons in quantum wells.

Yong Chen , Bernard Gil , Pierre Lefebvre , Henry Mathieu
Physical Review B: Condensed Matter and Materials Physics (1998-2015), 1988, 37 (11), pp.4. ⟨10.1103/PhysRevB.37.6429⟩
Article dans une revue hal-01175568 v1

Photo-manipulation of a liquid droplet by chromocapillary effect

Arnaud Saint-Jalmes , Antoine Diguet , Reine-Marie Guillermic , Damien Baigl , Yong Chen et al.
APS March meeting 2010, Mar 2010, Portland, United States
Communication dans un congrès hal-00661175 v1

Degree of polarization of surface-wave assisted thermal source

François Marquier , Christophe Arnold , Marine Laroche , Jean-Jacques Greffet , Yong Chen
Koli Workshop on Partial Electromagnetic Coherence and 3D Polarization, May 2009, Lieksa, Finland
Communication dans un congrès hal-00576147 v1

Micro-photoluminescence of GaN quantum dots embedded in 100 nm wide cylindrical AlN pillars

Thierry Taliercio , Sébastien Rousset , Pierre Lefebvre , Thierry Bretagnon , Thierry Guillet et al.
European Materials Research Society 2004, Symposium L. InN, GaN, AlN and Related Materials, their Heterostructures and Devices., European Materials Research Society, May 2004, Strasbourg, France. pp.783-790, ⟨10.1016/j.spmi.2004.09.034⟩
Communication dans un congrès istex hal-01312254 v1

Coherent Spontaneous Emission of Light Due to Surface Waves

Jean-Jacques Greffet , Rémi Carminati , Karl Joulain , Jean-Philippe Mulet , Carsten Henkel et al.
Tominaga J.; Tsai D.P. Optical Nanotechnologies, 88, Springer, Berlin, Heidelberg, pp.163-182 2003, Topics in Applied Physics 978-3-540-44070-3. ⟨10.1007/3-540-45871-9_12⟩
Chapitre d'ouvrage istex hal-01737968 v1