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Yvain Nicolet



Radical S -Adenosyl- l -Methionine Enzyme PylB: A C-Centered Radical to Convert l -Lysine into (3 R )-3-Methyl- d -Ornithine

Feryel Soualmia , Mickael Cherrier , Timothée Chauviré , Mickaël Mauger , Philip Tatham
Journal of the American Chemical Society, 2024, 146 (10), pp.6493-6505. ⟨10.1021/jacs.3c03747⟩
Journal articles hal-04496232v1

Structure and Catalytic Mechanism of Radical SAM Methylases

Tu-Quynh Nguyen , Yvain Nicolet
Life, 2022, 12 (11), pp.1732. ⟨10.3390/life12111732⟩
Journal articles hal-03853118v1

Characterization of a Radical SAM Oxygenase for the Ether Crosslinking in Darobactin Biosynthesis

Hai Nguyen , I Dewa Made Kresna , Nils Böhringer , Jeremie Ruel , Eugenio de La Mora
Journal of the American Chemical Society, 2022, ⟨10.1021/jacs.2c05565⟩
Journal articles hal-03807959v1

Iron–sulfur clusters as inhibitors and catalysts of viral replication

Kourosh Honarmand Ebrahimi , Simone Ciofi-Baffoni , Peter-Leon Hagedoorn , Yvain Nicolet , Nick Le Brun
Nature Chemistry, 2022, ⟨10.1038/s41557-021-00882-0⟩
Journal articles hal-03590147v1
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Oxygen-sensitive metalloprotein structure determination by cryo-electron microscopy

Mickaël Cherrier , Xavier Vernède , Daphna Fenel , Lydie Martin , Benoit Arragain
Biomolecules, 2022, 12 (3), pp.441. ⟨10.3390/biom12030441⟩
Journal articles hal-03615151v1

L-tyrosine-bound ThiH structure reveals C–C bond break differences within radical SAM aromatic amino acid lyases

Patricia Amara , Claire Saragaglia , Jean-Marie Mouesca , Lydie Martin , Yvain Nicolet
Nature Communications, 2022, 13 (1), pp.2284. ⟨10.1038/s41467-022-29980-4⟩
Journal articles hal-03669800v1

An Unexpected P-Cluster like Intermediate En Route to the Nitrogenase FeMo-co

Leon Jenner , Mickaël Cherrier , Patricia Amara , Luis Rubio , Yvain Nicolet
Chemical Science, 2021, ⟨10.1039/D1SC00289A⟩
Journal articles hal-03161940v1
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Radical SAM Enzymes and Metallocofactor Assembly: A Structural Point of View

Yvain Nicolet , Mickael Cherrier , Patricia Amara
ACS Bio & Med Chem Au, 2021, 2 (1), pp.36-52. ⟨10.1021/acsbiomedchemau.1c00044⟩
Journal articles hal-03454299v1

Methods to Screen for Radical SAM Enzyme Crystallization Conditions

Lydie Martin , Xavier Vernède , Yvain Nicolet
Methods in Molecular Biology, 2021, 2353, pp.333-348. ⟨10.1007/978-1-0716-1605-5_17⟩
Journal articles hal-03328451v1

Crystal structure of the [FeFe]-Hydrogenase maturase HydE bound to complex-B

Roman Rohac , Lydie Martin , Liang Liu , Debashis Basu , Lizhi Tao
Journal of the American Chemical Society, 2021, 143 (22), pp.8499-8508. ⟨10.1021/jacs.1c03367⟩
Journal articles hal-03328547v1

New developments in RiPP discovery, enzymology and engineering

Manuel Montalbán-López , Thomas Scott , Sangeetha Ramesh , Imran Rahman , Auke van Heel
Natural Product Reports, 2021, ⟨10.1039/d0np00027b⟩
Journal articles hal-02947402v1
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A way to thioacetate esters compatible with non-oxidative prebiotic conditions

Naoual Leqraa , Yvain Nicolet , Anne Milet , Yannick Vallée
Scientific Reports, 2020, 10 (1), pp.14488. ⟨10.1038/s41598-020-71524-7⟩
Journal articles hal-02934448v1

High-resolution structure of a modular hyperthermostable endo-β-1,4-mannanase from Thermotoga petrophila: The ancillary immunoglobulin-like module is a thermostabilizing domain

Viviam da Silva , Aline Cabral , Marcia Sperança , Fabio Squina , João Renato C Muniz
Biochimica et Biophysica Acta Proteins and Proteomics, 2020, 1868 (8), pp.140437. ⟨10.1016/j.bbapap.2020.140437⟩
Journal articles hal-02556971v1

Structural insights into the mechanism of the radical SAM carbide synthase NifB, a key nitrogenase cofactor maturating enzyme

Ana Sosa Fajardo , Pierre Legrand , Lucía Payá-Tormo , Lydie Martin , Maria Teresa Pellicer Martinez
Journal of the American Chemical Society, 2020, ⟨10.1021/jacs.0c02243⟩
Journal articles hal-02775951v1

Structure–function relationships of radical SAM enzymes

Yvain Nicolet
Nature Catalysis, 2020, 3, pp.337-350. ⟨10.1038/s41929-020-0448-7⟩
Journal articles hal-02547495v1

How [Fe]-hydrogenase metabolizes dihydrogen

Yvain Nicolet
Nature Catalysis, 2019, 2 (6), pp.481-482. ⟨10.1038/s41929-019-0296-5⟩
Journal articles hal-02166639v1

Human viperin catalyzes the modification of GPP and FPP potentially affecting cholesterol synthesis

Pavel Mikulecky , Elena Andreeva , Patricia Amara , Winfried Weissenhorn , Yvain Nicolet
FEBS Letters, 2018, ⟨10.1002/1873-3468.12941⟩
Journal articles hal-01677120v1

1,2-diol dehydration by the radical SAM enzyme AprD4 – a matter of proton circulation and substrate flexibility

Qi Liu , Patricia Amara , Jean-Marie Mouesca , Xinjian Ji , Oriane Renoux
Journal of the American Chemical Society, 2018, ⟨10.1021/jacs.7b10501⟩
Journal articles hal-01685058v1

Radical S -Adenosyl- l -methionine Tryptophan Lyase (NosL): How the Protein Controls the Carboxyl Radical •CO 2 – Migration

Patricia Amara , Jean-Marie Mouesca , Maxime Bella , Lydie Martin , Claire Saragaglia
Journal of the American Chemical Society, 2018, 140 (48), pp.16661-16668. ⟨10.1021/jacs.8b09142⟩
Journal articles hal-02008403v1

CO and CN- syntheses by [FeFe]-hydrogenase maturase HydG are catalytically differentiated events.

Adrien Pagnier , Lydie Martin , Laura Zeppieri , Yvain Nicolet , Juan-Carlos Fontecilla-Camps
Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (1), pp.104-9
Journal articles hal-01252697v1

Fine-tuning of a radical-based reaction by radical S-adenosyl-L-methionine tryptophan lyase

G. Sicoli , J.-M. Mouesca , Laura Zeppieri , Patricia Amara , Lydie Martin
Science, 2016, 351 (6279), pp.1320-1323. ⟨10.1126/science.aad8995⟩
Journal articles hal-01297885v1

Carbon-sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE.

Roman Rohac , Patricia Amara , Alhosna Benjdia , Lydie Martin , Pauline Ruffié
Nature Chemistry, 2016, 8 (5), pp.491-500. ⟨10.1038/NCHEM.2490⟩
Journal articles hal-01341083v1

The crystal structure of the global anaerobic transcriptional regulator FNR explains its extremely fine-tuned monomer-dimer equilibrium

Anne Volbeda , Claudine Darnault , O. Renoux , Yvain Nicolet , Juan-Carlos Fontecilla-Camps
Science Advances , 2015, 1 (11), pp.e1501086. ⟨10.1126/sciadv.1501086⟩
Journal articles hal-01240354v1

IscS from Archaeoglobus fulgidus has no desulfurase activity but may provide a cysteine ligand for [Fe2S2] cluster assembly.

Adrien Pagnier , Yvain Nicolet , Juan-Carlos Fontecilla-Camps
BBA - Biochimica et Biophysica Acta, 2015, 1853 (6), pp.1457-1463
Journal articles hal-01119798v1

Tryptophan Lyase (NosL): Mechanistic Insights from Substrate Analogues and Mutagenesis.

Dhananjay M Bhandari , Hui Xu , Yvain Nicolet , Juan-Carlos Fontecilla-Camps , Tadhg P Begley
Biochemistry, 2015, 54 (31), pp.4767-9
Journal articles hal-01191593v1

Crystal structure of HydG from Carboxydothermus hydrogenoformans: a trifunctional [FeFe]-hydrogenase maturase.

Yvain Nicolet , Adrien Pagnier , Laura Zeppieri , Lydie Martin , Patricia Amara
ChemBioChem, 2015, 16 (3), pp.397-402
Journal articles hal-01166126v1

Crystal structure of tryptophan lyase (NosL): evidence for radical formation at the amino group of tryptophan.

Yvain Nicolet , Laura Zeppieri , Patricia Amara , Juan-Carlos Fontecilla-Camps
Angewandte Chemie International Edition, 2014, 53 (44), pp.11840-4
Journal articles hal-01119795v1

X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters

Yvain Nicolet , Roman Rohac , Lydie Martin , Juan-Carlos Fontecilla-Camps
Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 (18), pp.7188 - 7192. ⟨10.1073/pnas.1302388110⟩
Journal articles hal-01664850v1

Crystal structure and functional studies of an unusual l -cysteine desulfurase from Archaeoglobus fulgidus

Yasuaki Yamanaka , Laura Zeppieri , Yvain Nicolet , Elodie N Marinoni , Jaim S de Oliveira
Dalton Transactions, 2013, 42 (9), pp.3092 - 3099. ⟨10.1039/c2dt32101g⟩
Journal articles hal-01666076v1

Crystal structures of human cholinesterases in complex with huprine W and tacrine: elements of specificity for anti-Alzheimer's drugs targeting acetyl- and butyryl-cholinesterase.

Florian Nachon , Eugénie Carletti , Cyril Ronco , Marie Trovaslet , Yvain Nicolet
Biochemical Journal, 2013, 453 (3), pp.393-399. ⟨10.1042/BJ20130013⟩
Journal articles hal-00996550v1

Further Characterization of the [FeFe]-Hydrogenase Maturase HydG

Cécile Tron , Mickael V Cherrier , Patricia Amara , Lydie Martin , Martin Fauth
European Journal of Inorganic Chemistry, 2011, 2011 (7 SI), pp.1121-1127. ⟨10.1002/ejic.201001101⟩
Journal articles hal-01140285v1

Reactibodies generated by kinetic selection couple chemical reactivity with faborable protein dynamics

Ivan Smirnov , Eugénie Carletti , Inna Kurkova , Florian Nachon , Yvain Nicolet
Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (38), pp.15954-15959. ⟨10.1073/pnas.1108460108⟩
Journal articles hal-00637416v1
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X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger

Florian Nachon , Eugenie Carletti , Marielle Wandhammer , Yvain Nicolet , Lawrence M Schopfer
Biochemical Journal, 2011, 434 (1), pp.73-82. ⟨10.1042/BJ20101648⟩
Journal articles hal-00560692v1

A glycyl free radical as the precursor in the synthesis of carbon monoxide and cyanide by the [FeFe]-hydrogenase maturase HydG

Yvain Nicolet , Lydie Martin , Cécile Tron , Juan-Carlos Fontecilla-Camps
FEBS Letters, 2010, 584 (19), pp.4197-4202. ⟨10.1016/j.febslet.2010.09.008⟩
Journal articles hal-01235598v1

Maturation of [FeFe]-hydrogenases: Structures and mechanisms

Yvain Nicolet , Juan-Carlos Fontecilla-Camps , Marc Fontecave
International Journal of Hydrogen Energy, 2010, 35 (19), pp.10750-10760. ⟨10.1016/j.ijhydene.2010.02.056⟩
Journal articles hal-01145486v1
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Structure-activity analysis of aging and reactivation of human butyrylcholinesterase inhibited by analogues of tabun

Eugénie Carletti , Nadine Aurbek , Emilie Gillon , Mélanie Loiodice , Yvain Nicolet
Biochemical Journal, 2009, 421 (1), pp.97-106. ⟨10.1042/BJ20090091⟩
Journal articles hal-00479152v1

Structure-function relationships of anaerobic gas-processing metalloenzymes

Juan-Carlos Fontecilla-Camps , Patricia Amara , Christine Cavazza , Yvain Nicolet , Anne Volbeda
Nature, 2009, 460 (7257), pp.814-822. ⟨10.1038/nature08299⟩
Journal articles cea-00909652v1

The role of the maturase HydG in [FeFe]-hydrogenase active site synthesis and assembly

Eric Pilet , Yvain Nicolet , Carole Mathevon , Thierry Douki , Juan-Carlos Fontecilla-Camps
FEBS Letters, 2009, 583 (3), pp.506-511
Journal articles hal-00371635v1

X-ray structure of the [Fe-Fe]-hydrogenase maturase hydE from thermotoga maritima.

Yvain Nicolet , Jon K. Ruback , Matthew C. Posewitz , Patricia Amara , Carole Mathevon
Journal of Biological Chemistry, 2008, 283 (27), pp.18861-18872
Journal articles hal-00377680v1

Characterization of the DNA repair spore photoproduct lyase enzyme from Clostridium acetobutylicum: A radical-SAM enzyme.

Alexia Chandor , Thierry Douki , Didier Gasparutto , Serge Gambarelli , Yiannis Sanakis
Comptes Rendus. Chimie, 2007, 10, pp.756-765
Journal articles hal-00377642v2
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Structural Changes of Escherichia coli Ferric Uptake Regulator during Metal-dependent Dimerization and Activation Explored by NMR and X-ray Crystallography

Ludovic Pecqueur , Benoit d'Autreaux , Jérôme Dupuy , Yvain Nicolet , Lilian Jacquamet
Journal of Biological Chemistry, 2006, 281 (30), pp.21286-21295. ⟨10.1074/jbc.M601278200⟩
Journal articles hal-02975695v1