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Badreddine Douzi

Badreddine Douzi CRCN INRAE
Researcher identifiers


- **Career Biography** Once he obtained a diploma in Biotechnology Engineering from University of Sfax in Tunisia, Badreddine Douzi moved to France to start a scientific career in the field of microbiology. He studied biochemistry, structural biology and molecular biology at the University of Aix-Marseille and obtained his Master degree. Then he earned a PhD in biochemistry and structural Biology under the supervision of Dr Romé Voulhoux and Dr Mariella Tegoni. His thesis work was dedicated to the study of molecular mechanisms underlying the assembly and function of a bacterial nanomachine called the type II secretion system (T2SS). Fascinated by these bacterial nanomachines, he was interested during his first postdoctorat, under the supervision of Dr Christian Cambillau, by studying a different secretion system called the type VI secretion system (T6SS) dedicated to the injection of toxins into neighboring cells. Then he moved to the University of British Columbia in the laboratory of Prof Franck Duong to study the molecular mechanisms that coordinates the translocation of colicins across the bacterial envelope. In 2015, he went back to France to join the team of Dr Romé Voulhoux as research associate to work on the T2SS, specifically on the assembly and function of the portal complex called the Secretin. After a short period on the laboratory of Dr Etienne Maisonneuve, Badreddine Douzi was appointed “Chargé de Recherche at the INRA in 2018 in the DynAMic laboratory. - **Research interest** Working on bacterial nanomachines since 2008, Badreddine Douzi main’s interests are to understand, at molecular levels, the mechanisms underlying the traffic of molecules across the bacterial envelope. The transport of molecules is orchestrated by a specialized bacterial apparatus called secretion systems. They are large macromolecular complexes dedicated to the transport of proteins and DNA to the extracellular milieu or directly to host cells. Among the nine secretion systems identified up to date, Badreddine Douzi is currently focused on the study of the assembly and function of the conjugative type IV secretion system (T4SS) in Gram + bacteria. These nanomachines are responsible for the transfer of mobile genetic elements between bacteria and consequently play a crucial role in horizontal gene transfer. He is using complementary approaches (genetics, molecular biology, biochemistry and structural biology) to understand how these nanomachines assembles on the bacterial envelope and ensure the DNA transfer.


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Unravelling HetC as a peptidase-based ABC exporter driving functional cell differentiation in the cyanobacterium Nostoc PCC 7120

Raphaël Rachedi , Véronique Risoul , Maryline Foglino , Yanis Aoudache , Kevin Lang
Microbiology Spectrum, 2024, 12 (4), ⟨10.1128/spectrum.04058-23⟩
Journal articles hal-04539483v1

Refining the Dynamic Network of T2SS Endopilus Tip Heterocomplex Combining cw‐EPR and Nitroxide–Gd III Distance Measurements

Guillaume Gerbaud , Brice Barbat , Mathilde Tribout , Emilien Etienne , Valérie Belle
ChemBioChem, 2023, 24 (15), ⟨10.1002/cbic.202300099⟩
Journal articles hal-04210232v1
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Insights into dynamics and gating properties of T2SS secretins

Brice Barbat , Badreddine Douzi , Geneviève Ball , Mathilde Tribout , Khalid El Karkouri
Science Advances , 2023, 9 (40), ⟨10.1126/sciadv.adg6996⟩
Journal articles hal-04254401v1
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Structural lessons on bacterial secretins

Brice Barbat , Badreddine Douzi , Romé Voulhoux
Biochimie, 2022, 205, pp.110-116. ⟨10.1016/j.biochi.2022.08.019⟩
Journal articles hal-03845257v1

A new twin expands the VirB8-like protein family

Robine Maffo-Woulefack , Nathalie N. Leblond-Bourget , Badreddine Douzi
Structure, 2022, 30 (6), pp.790-792. ⟨10.1016/j.str.2022.05.004⟩
Journal articles hal-03994713v1
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The crystal structure of CbpD clarifies substrate-specificity motifs in chitin-active lytic polysaccharide monooxygenases

Christopher Dade , Badreddine Douzi , Christian Cambillau , Genevieve Ball , Romé Voulhoux
Acta crystallographica Section D : Structural biology [1993-..], 2022, 78 (8), pp.1064-1078. ⟨10.1107/S2059798322007033⟩
Journal articles hal-03739502v1
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Structural interactions define assembly adapter function of a type II secretion system pseudopilin

Cristian Escobar , Badreddine Douzi , Geneviève Ball , Brice Barbat , Sebastien Alphonse
Structure, 2021, 29 (10), pp.1116-1127.e8. ⟨10.1016/j.str.2021.05.015⟩
Journal articles hal-03367306v1
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Structural and Biochemical Analysis of OrfG: The VirB8-like Component of the Conjugative Type IV Secretion System of ICESt3 From Streptococcus thermophilus

Julien Cappele , Abbas Mohamad Ali , Nathalie N. Leblond-Bourget , Sandrine Mathiot , Tiphaine Dhalleine
Frontiers in Molecular Biosciences, 2021, 8, ⟨10.3389/fmolb.2021.642606⟩
Journal articles hal-03196531v1
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HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacterium Nostoc PCC 7120

Xiaomei Xu , Véronique Risoul , Deborah Byrne , Stéphanie M Champ , Badreddine Douzi
Journal articles hal-02917356v1
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YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli

Elsa Germain , Paul Guiraud , Deborah Byrne , Badreddine Douzi , Meriem Djendli
Nature Communications, 2019, 10 (1), ⟨10.1038/s41467-019-13764-4⟩
Journal articles hal-02446175v1
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Towards a complete structural deciphering of Type VI secretion system

van Son Nguyen , Badreddine Douzi , Eric Durand , Alain Roussel , E. Cascales
Current Opinion in Structural Biology, 2018, 49, pp.77 - 84. ⟨10.1016/⟩
Journal articles hal-01780753v1
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Direct interactions between the secreted effector and the T2SS components GspL and GspM reveal a new effector-sensing step during type 2 secretion

Sandra Michel-Souzy , Badreddine Douzi , Frederic Cadoret , Claire Raynaud , Loïc Quinton
Journal of Biological Chemistry, 2018, 293 (50), pp.19441-19450. ⟨10.1074/jbc.RA117.001127⟩
Journal articles hal-01919531v1
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The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly

Melvin G Renault , Jordi Zamarreño Beas , Badreddine Douzi , Maïalène Chabalier , Abdelrahim Zoued
Journal of Molecular Biology, 2018, 430 (18 Pt B), pp.3143-3156. ⟨10.1016/j.jmb.2018.07.018⟩
Journal articles hal-01847145v1
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Structure–Function Analysis of the C-Terminal Domain of the Type VI Secretion TssB Tail Sheath Subunit

Badreddine Douzi , Laureen Logger , Silvia Spinelli , Stephanie Blangy , Christian Cambillau
Journal of Molecular Biology, 2018, 430 (3), pp.297 - 309. ⟨10.1016/j.jmb.2017.11.015⟩
Journal articles hal-01780748v1
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Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics

Badreddine Douzi , Nhung Trinh , Sandra Michel-Souzy , Aline Desmyter , Genevieve Ball
mBio, 2017, 8 (5), ⟨10.1128/mBio.01185-17⟩
Journal articles hal-01744494v1
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Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17"

Abdelrahim Zoued , Eric Durand , Yannick R Brunet , Silvia Spinelli , Badreddine Douzi
Nature, 2016, 531 (7592), pp.59 - 63. ⟨10.1038/nature17182⟩
Journal articles hal-01778575v1
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Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli

Badreddine Douzi , Yannick R Brunet , Silvia Spinelli , Valentine Lensi , Stephanie Blangy
Scientific Reports, 2016, 6 (1), ⟨10.1038/srep34405⟩
Journal articles hal-01780690v1
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Structure–Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes

Abdelrahim Zoued , Chloé J Cassaro , Eric Durand , Badreddine Douzi , Alexandre España
Journal of Molecular Biology, 2016, 428 (22), pp.4413 - 4423. ⟨10.1016/j.jmb.2016.08.030⟩
Journal articles hal-01780163v1
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The Type VI Secretion TssEFGK-VgrG Phage-Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves As Assembly Platform for Tail Tube/Sheath Polymerization

Yannick R Brunet , Abdelrahim Zoued , Frédéric Boyer , Badreddine Douzi , E. Cascales
PLoS Genetics, 2015, 11 (10), pp.e1005545. ⟨10.1371/journal.pgen.1005545⟩
Journal articles hal-01458191v1
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Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody

van Son Nguyen , Laureen Logger , Silvia Spinelli , Aline Desmyter , Thi Thu Hang Le
PLoS ONE, 2015, 10 (e0122187), pp.1-14
Journal articles hal-01217205v1

Txc, a new type II secretion system of Pseudomonas aeruginosa strain PA7, is regulated by the TtsS/TtsR two-component system and directs specific secretion of the CbpE chitin-binding protein.

Frederic Cadoret , Geneviève Ball , Badreddine Douzi , Romé Voulhoux
Journal of Bacteriology, 2014, 196 (13), pp.2376--86. ⟨10.1128/JB.01563-14⟩
Journal articles hal-01458218v1
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Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli

Badreddine Douzi , Silvia Spinelli , Stéphanie Blangy , Alain Roussel , Eric Durand
PLoS ONE, 2014, 9 (2), pp.e86918. ⟨10.1371/journal.pone.0086918⟩
Journal articles hal-02091448v1

Architecture and assembly of the Type VI secretion system.

Abdelrahim Zoued , Yannick R Brunet , Eric Durand , Marie-Stéphanie Aschtgen , Laureen Logger
Biochim. Biophys. Acta, 2014, 1843 (8), pp.1664--73. ⟨10.1016/j.bbamcr.2014.03.018⟩
Journal articles hal-01458220v1
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TssK Is a Trimeric Cytoplasmic Protein Interacting with Components of Both Phage-like and Membrane Anchoring Complexes of the Type VI Secretion System

Abdelrahim Zoued , Eric Durand , Cecilia C. Bebeacua , Yannick R Brunet , Badreddine Douzi
Journal of Biological Chemistry, 2013, 288 (38), pp.27031-27041. ⟨10.1074/jbc.M113.499772⟩
Journal articles hal-02091435v1
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Dissection of the TssB-TssC Interface during Type VI Secretion Sheath Complex Formation

Xiang Y. Zhang , Yannick R Brunet , Laureen Logger , Badreddine Douzi , Christian Cambillau
PLoS ONE, 2013, 8 (11), pp.e81074. ⟨10.1371/journal.pone.0081074⟩
Journal articles hal-02091436v1
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High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

Hervé Nozach , Carole Fruchart-Gaillard , François Fenaille , Fabrice Beau , Oscar Pereira Ramos
Microbial Cell Factories, 2013, 12, ⟨10.1186/1475-2859-12-37⟩
Journal articles cea-02100032v1

On the path to uncover the bacterial type II secretion system.

Badreddine Douzi , Alain Filloux , Romé Voulhoux
Philos. Trans. R. Soc. Lond., B, Biol. Sci., 2012, 367 (1592), pp.1059--72. ⟨10.1098/rstb.2011.0204⟩
Journal articles hal-01458255v1
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Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar

Catarina Felisberto-Rodrigues , Eric Durand , Marie-Stéphanie Aschtgen , Stephanie Blangy , Miguel Ortiz-Lombardia
PLoS Pathogens, 2011, 7 (11), pp.e1002386. ⟨10.1371/journal.ppat.1002386⟩
Journal articles hal-01458272v1

Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates.

Badreddine Douzi , Geneviève Ball , Christian Cambillau , Mariella Tegoni , Romé Voulhoux
Journal of Biological Chemistry, 2011, 286 (47), pp.40792--801. ⟨10.1074/jbc.M111.294843⟩
Journal articles hal-01458270v1

Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system.

Laura P Franz , Badreddine Douzi , Eric Durand , David H Dyer , Romé Voulhoux
Acta Crystallogr. D Biol. Crystallogr., 2011, 67 (Pt 2), pp.124--30. ⟨10.1107/S0907444910051954⟩
Journal articles hal-01458266v1

The Assembly Mode of the Pseudopilus: a hallmark to distinguish a novel secretion system subtype

E. Durand , S. Alphonse , Céline Brochier-Armanet , G. Ball , Badreddine Douzi
Journal of Biological Chemistry, 2011, 286 (27), pp.24407-24416. ⟨10.1074/jbc.M111.234278⟩
Journal articles hal-02299276v1
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D-Maurocalcine, a pharmacologically inert efficient cell-penetrating peptide analogue.

Cathy Poillot , Kaouthar Dridi , Hicham Bichraoui , Julien Pêcher , Sébastien Alphonse
Journal of Biological Chemistry, 2010, 285 (44), pp.34168-80. ⟨10.1074/jbc.M110.104919⟩
Journal articles inserm-00587800v1

Structure of the Pseudomonas aeruginosa XcpT pseudopilin, a major component of the type II secretion system

S. Alphonse , E. Durand , Badreddine Douzi , B. Waegele , H. Darbon
Journal of Structural Biology, 2010, 169 (1), pp.75-80. ⟨10.1016/j.jsb.2009.09.003⟩
Journal articles hal-02667520v1

The XcpV/GspI pseudopilin has a central role in the assembly of a quaternary complex within the T2SS pseudopilus

Badreddine Douzi , E. Durand , C. Bernard , S. Alphonse , C. Cambillau
Journal of Biological Chemistry, 2009, 284 (50), pp.34580-34589. ⟨10.1074/jbc.M109.042366⟩
Journal articles hal-02666049v1

Nanobodies to investigate the architecture and dynamics of the Type II secretion nanomachine

Badreddine Douzi
Third FRISBI annual user meeting, Nov 2017, paris, France
Conference papers hal-02791428v1

Nanobodies uncover a new transport pathway of the secretin XcpQ to the outer membrane

Badreddine Douzi
JSM3 Junior Scientist Microbiology, May 2016, marseille, France
Conference papers hal-02744297v1

Deciphering the Type II secretion system dynamics and function

Badreddine Douzi
EMBO-Clostridial Secretion, Sep 2012, marseille, France
Conference papers hal-02750355v1

Protein-Protein Interactions: Surface Plasmon Resonance

Badreddine Douzi
Bacterial Protein Secretion Systems : Methods and Protocols, Springer Link, 2017, Methods in Molecular Biology, 978-1-4939-7033-9 978-1-4939-7031-5. ⟨10.1007/978-1-4939-7033-9_21⟩
Book sections hal-02790765v1
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La machinerie de sécrétion de type II Xcp de pseudomonas aeruginosa

Badreddine Douzi
Sciences du Vivant [q-bio]. Université de Provence, 2011. Français. ⟨NNT : ⟩
Theses tel-02806009v1