Béatrice Roche

15
Documents

Présentation

EDUCATION

2015 Ph.D in microbiology, University of Aix-Marseille, IMM, France.

2011 Postgraduate engineer, Polytech School of Advanded Studies in Engineering of Luminy, Marseille, France.

2008 Bachelor's degree in cellular biology, University of Aix-Marseille, France

RESEARCH EXPERIENCES

2019-2022 Post-doctoral researcher, University of Basel, Biozentrum; Infection Biology, Basel; Switzerland. Director: Pr Dirk BUMANN.

2016-2019 Post-doctoral researcher, University of Strasbourg-CNRS, ESBS, UMR 7242, Illkirch, France. Director: Dr Isabelle SCHALK.

2011-2015 Ph.D in microbiology, LCB, Marseille, France. Director: Pr Frédéric BARRAS.

2011 Engineering internship, Nosopharm Biotech company, Nîmes, France.

MENTORING

2019-2020 Master, Jasmin Künnecke, Biozentrum, Basel, Switzerland

2017-2019 Ph.D student, Anne Bonneau, ESBS, Illkirch, France. MRT fellowship.

2017 Master, Anne Bonneau, ESBS, Illkirch, France.

2017 Training in protein purification in liquid chromatography, CNRS-ESBS, Illkirch, France.

GRANTS/FUNDINGS

2018 Instruct (PID 6435), Macromolecular crystallization, 1500 €

2017 FRISBI funding, Electron microscopy, 500 €

2017 Instruct funding (PID 2886), Electron microscopy, 1500 €

2016-2019 Hoffmann-La Roche post-doctoral fellowship

2015 Foundation for Medical Research (FRM), 4th year PhD salary

2011-2015 French Ministry of Advanced Instruction and Research, Ph.D fellowship

MAJOR SKILLS

Writing: fundings and research articles (12) including 6 first author and 3 corresponding author.

Review Editor in Frontiers in Microbiology – Infectious Agents and Disease (from Nov 2021)

Molecular biology: overlap PCR, gene cloning, construction of deletion mutant, PCR mutagenesis, RNA extraction.

Microbiology: E. coli and BSL2 pathogens (P. aeruginosa, S. enterica), 55Fe uptake assay, conjugation, antibiotic killing.

Biochemistry: protein production and purification (liquid chromatography), purification of membrane proteins, electrophoresis SDS-PAGE, immunodetection assays.

Biophysics/microscopy: flow-cytometry, phase contrast and fluorescence microscopy, negative-stain EM data processing with Relion2, EMAN2.1, Chimera.

Animal skills: mice handling, iv injection (FELASA number LTK4727)

PUBLICATIONS

1. Roche B *, Garcia-Rivera MA, Normant V, Kuhn L, Hammann P, Brönstrup M, Mislin GLA, Schalk IJ *. A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa. Environ Microbiol. 2021. doi: 10.1111/1462-2920.15811

2. Roche B *, Mislin GLA and Schalk IJ. Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway. FEBS Lett. 2020; 595: 370-378.

3. Roche B and Bumann D *. Single-cell reporters for pathogen responses to antimicrobial host attacks. Curr Opin Microbiol. 2020; 59:16-23.

4. Perraud Q, Cantero P, Roche B, Gasser V, Normant VP, Kuhn L, Hammann P, Mislin GLA, Ehret-Sabatier L and Schalk IJ *. Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms. Mol Cell Proteomics. 2020;19: 589–607.

5. Bonneau A, Roche B *, Schalk IJ *. Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins. Sci Rep. 2020;10: 120.

6. Paulen A, Hoegy F, Roche B, Schalk IJ, Mislin GLA *. Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue. Bioorg Med Chem Lett. 2017;27: 4867–4870.

7. Ganne G, Brillet K, Basta B, Roche B, Hoegy F, Gasser V and Schalk IJ *. Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH. ACS Chem Biol. 2017;12: 1056–1065.

8. Roche B, Agrebi R, Huguenot A, Ollagnier de Choudens S, Barras F, Py B *. Turning Escherichia coli into a Frataxin-Dependent Organism. PLoS Genet. 2015;11: e1005134.

9. Roche B, Huguenot A, Barras F, Py B *. The iron-binding CyaY and IscX proteins assist the ISC-catalyzed Fe-S biogenesis in Escherichia coli. Mol Microbiol. 2015;95: 605–623.

10. Yan R, Konarev PV, Iannuzzi C, Adinolfi S, Roche B, Kelly G, et al. Ferredoxin competes with bacterial frataxin in binding to the desulfurase IscS. J Biol Chem. 2013;288: 24777–24787.

11. Roche B, Aussel L, Ezraty B, Mandin P, Py B, Barras F *. Iron/sulfur proteins biogenesis in prokaryotes: formation, regulation and diversity. Biochim Biophys Acta. 2013;1827: 455–469.

12. Bordi C, Lamy M-C, Ventre I, Termine E, Hachani A, Fillet S, Roche B, Bleves S, et al. Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis. Mol Microbiol. 2010;76: 1427–1443.

* = corresponding author

SCIENTIFIC COMMUNICATIONS

2022&2021-Biozentrum, Basel, Switzerland ; 2020-LBP, Illkirch, France ; **2020-**Biozentrum, Basel, Switzerland ; 2018-Institut Pasteur, Paris, France ; 2017-Hoffmann-la Roche, Basel, Switzerland ; 2017-ESBS, Illkirch, France ; 2016-ESBS, Illkirch, France ; 2015-University of Uppsala, Sweden, EMBO fellowship interview ; 2015-Norwich, UK ; 2014-MRC, UK ; 2013- University of South Carolina, USA ; 2013-LCB, Marseille, France.

Publications

Publications

Image document

Shigella sonnei utilises colicins during inter-bacterial competition

P. Leung , X. Matanza , B. Roche , K. Ha , H. Cheung et al.
Microbiology, 2024, 170 (2), ⟨10.1099/mic.0.001434⟩
Article dans une revue hal-04772330 v1
Image document

SPATEs promote the survival of Shigella to the plasma complement system upon local hemorrhage and bacteremia

Lorine Debande , Ahmad Sabbah , Lauriane Kuhn , Richard Patryk Ngondo , Julie Maucotel et al.
Proceedings of the National Academy of Sciences of the United States of America, 2024, 121 (45), ⟨10.1073/pnas.2319951121⟩
Article dans une revue hal-05021137 v1

Heterogeneous dual-metal control of Salmonella infection

Béatrice Roche , Olivier Cunrath , Christopher Bleck , Beatrice Claudi , Minia Antelo Varela et al.
Article dans une revue hal-04772335 v1

SPATEs promote the survival of Shigella to the plasma complement system upon local hemorrhage and bacteremia

Lorine Debande , Ahmad Sabbah , Laurianne Kuhn , Patryk Ngondo , Antonin André et al.
Article dans une revue hal-04587887 v1
Image document

Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway

Béatrice Roche , Gaëtan L.A. Mislin , Isabelle J Schalk
FEBS Letters, In press, ⟨10.1002/1873-3468.14012⟩
Article dans une revue hal-03039826 v1

Single-cell reporters for pathogen responses to antimicrobial host attacks

Béatrice Roche , Dirk Bumann
Current Opinion in Microbiology, 2021, 59, pp.16-23. ⟨10.1016/j.mib.2020.07.013⟩
Article dans une revue hal-04772337 v1

A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa

Béatrice Roche , Mariel Garcia-Rivera , Vincent Normant , Lauriane Kuhn , Philippe Hammann et al.
Environmental Microbiology, 2021, 24 (2), pp.866-877. ⟨10.1111/1462-2920.15811⟩
Article dans une revue hal-04772336 v1
Image document

Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins

Anne Bonneau , Béatrice Roche , Isabelle Schalk
Scientific Reports, 2020, 10 (1), ⟨10.1038/s41598-019-56913-x⟩
Article dans une revue hal-02982784 v1
Image document

Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms

Quentin Perraud , Paola Cantero , Béatrice Roche , Véronique Gasser , Vincent Normant et al.
Molecular and Cellular Proteomics, 2020, 19 (4), pp.589-607. ⟨10.1074/mcp.RA119.001829⟩
Article dans une revue hal-02555371 v1

Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue

Aurélie Paulen , Françoise. Hoegy , Béatrice. Roche , Isabelle Schalk , Gaëtan L.A. Mislin
Bioorganic and Medicinal Chemistry Letters, 2017, 27 (21), pp.4867-4870. ⟨10.1016/j.bmcl.2017.09.039⟩
Article dans une revue hal-02348563 v1
Image document

Iron Release from the Siderophore Pyoverdine in Involves Three New Actors: FpvC, FpvG, and FpvH

Géraldine Ganne , Karl Brillet , Beata Szafarowicz Basta , Béatrice Roche , Françoise Hoegy et al.
ACS Chemical Biology, 2017, 12 (4), pp.1056-1065. ⟨10.1021/acschembio.6b01077⟩
Article dans une revue hal-02348567 v1

The iron-binding CyaY and IscX proteins assist the ISC-catalyzed Fe-S biogenesis in Escherichia coli

Beatrice Roche , Allison Huguenot , Frederic Barras , Beatrice Py
Molecular Microbiology, 2015, 95 (4), pp.605--623. ⟨10.1111/mmi.12888⟩
Article dans une revue hal-01452060 v1
Image document

Turning Escherichia coli into a Frataxin-Dependent Organism

Béatrice Roche , Rym Agrebi , Allison Huguenot , Sandrine Ollagnier de Choudens , Frédéric Barras et al.
PLoS Genetics, 2015, 11 (5), pp.UNSP e1005134. ⟨10.1371/journal.pgen.1005134⟩
Article dans une revue hal-01213670 v1

Reprint of: Iron/sulfur proteins biogenesis in prokaryotes: Formation, regulation and diversity

Béatrice Roche , Laurent Aussel , Benjamin Ezraty , Pierre Mandin , Béatrice Py et al.
Biochimica biophysica acta (BBA) - Bioenergetics, 2013, 1827 (8-9), pp.923-937. ⟨10.1016/j.bbabio.2013.05.001⟩
Article dans une revue hal-03616227 v1

Ferredoxin Competes with Bacterial Frataxin in Binding to the Desulfurase IscS*

Robert Yan , Petr Konarev , Clara Iannuzzi , Salvatore Adinolfi , Béatrice Roche et al.
Journal of Biological Chemistry, 2013, 288 (34), pp.24777-24787. ⟨10.1074/jbc.M113.480327⟩
Article dans une revue hal-03616114 v1