Béatrice Roche
Présentation
EDUCATION 2015 Ph.D in microbiology, University of Aix-Marseille, IMM, France. 2011 Postgraduate engineer, Polytech School of Advanded Studies in Engineering of Luminy, Marseille, France. 2008 Bachelor's degree in cellular biology, University of Aix-Marseille, France RESEARCH EXPERIENCES 2019-2022 Post-doctoral researcher, University of Basel, Biozentrum; Infection Biology, Basel; Switzerland. Director: Pr Dirk BUMANN. 2016-2019 Post-doctoral researcher, University of Strasbourg-CNRS, ESBS, UMR 7242, Illkirch, France. Director: Dr Isabelle SCHALK. 2011-2015 Ph.D in microbiology, LCB, Marseille, France. Director: Pr Frédéric BARRAS. 2011 Engineering internship, Nosopharm Biotech company, Nîmes, France. MENTORING 2019-2020 Master, Jasmin Künnecke, Biozentrum, Basel, Switzerland 2017-2019 Ph.D student, Anne Bonneau, ESBS, Illkirch, France. MRT fellowship. 2017 Master, Anne Bonneau, ESBS, Illkirch, France. 2017 Training in protein purification in liquid chromatography, CNRS-ESBS, Illkirch, France. GRANTS/FUNDINGS 2018 Instruct (PID 6435), Macromolecular crystallization, 1500 € 2017 FRISBI funding, Electron microscopy, 500 € 2017 Instruct funding (PID 2886), Electron microscopy, 1500 € 2016-2019 Hoffmann-La Roche post-doctoral fellowship 2015 Foundation for Medical Research (FRM), 4th year PhD salary 2011-2015 French Ministry of Advanced Instruction and Research, Ph.D fellowship MAJOR SKILLS Writing: fundings and research articles (12) including 6 first author and 3 corresponding author. Review Editor in Frontiers in Microbiology – Infectious Agents and Disease (from Nov 2021) Molecular biology: overlap PCR, gene cloning, construction of deletion mutant, PCR mutagenesis, RNA extraction. Microbiology: E. coli and BSL2 pathogens (P. aeruginosa, S. enterica), 55Fe uptake assay, conjugation, antibiotic killing. Biochemistry: protein production and purification (liquid chromatography), purification of membrane proteins, electrophoresis SDS-PAGE, immunodetection assays. Biophysics/microscopy: flow-cytometry, phase contrast and fluorescence microscopy, negative-stain EM data processing with Relion2, EMAN2.1, Chimera. Animal skills: mice handling, iv injection (FELASA number LTK4727) PUBLICATIONS 1. Roche B *, Garcia-Rivera MA, Normant V, Kuhn L, Hammann P, Brönstrup M, Mislin GLA, Schalk IJ *. A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa. Environ Microbiol. 2021. doi: 10.1111/1462-2920.15811 2. Roche B *, Mislin GLA and Schalk IJ. Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway. FEBS Lett. 2020; 595: 370-378. 3. Roche B and Bumann D *. Single-cell reporters for pathogen responses to antimicrobial host attacks. Curr Opin Microbiol. 2020; 59:16-23. 4. Perraud Q, Cantero P, Roche B, Gasser V, Normant VP, Kuhn L, Hammann P, Mislin GLA, Ehret-Sabatier L and Schalk IJ *. Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms. Mol Cell Proteomics. 2020;19: 589–607. 5. Bonneau A, Roche B *, Schalk IJ *. Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins. Sci Rep. 2020;10: 120. 6. Paulen A, Hoegy F, Roche B, Schalk IJ, Mislin GLA *. Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue. Bioorg Med Chem Lett. 2017;27: 4867–4870. 7. Ganne G, Brillet K, Basta B, Roche B, Hoegy F, Gasser V and Schalk IJ *. Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH. ACS Chem Biol. 2017;12: 1056–1065. 8. Roche B, Agrebi R, Huguenot A, Ollagnier de Choudens S, Barras F, Py B *. Turning Escherichia coli into a Frataxin-Dependent Organism. PLoS Genet. 2015;11: e1005134. 9. Roche B, Huguenot A, Barras F, Py B *. The iron-binding CyaY and IscX proteins assist the ISC-catalyzed Fe-S biogenesis in Escherichia coli. Mol Microbiol. 2015;95: 605–623. 10. Yan R, Konarev PV, Iannuzzi C, Adinolfi S, Roche B, Kelly G, et al. Ferredoxin competes with bacterial frataxin in binding to the desulfurase IscS. J Biol Chem. 2013;288: 24777–24787. 11. Roche B, Aussel L, Ezraty B, Mandin P, Py B, Barras F *. Iron/sulfur proteins biogenesis in prokaryotes: formation, regulation and diversity. Biochim Biophys Acta. 2013;1827: 455–469. 12. Bordi C, Lamy M-C, Ventre I, Termine E, Hachani A, Fillet S, Roche B, Bleves S, et al. Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis. Mol Microbiol. 2010;76: 1427–1443. * = corresponding author SCIENTIFIC COMMUNICATIONS 2022&2021-Biozentrum, Basel, Switzerland ; 2020-LBP, Illkirch, France ; **2020-**Biozentrum, Basel, Switzerland ; 2018-Institut Pasteur, Paris, France ; 2017-Hoffmann-la Roche, Basel, Switzerland ; 2017-ESBS, Illkirch, France ; 2016-ESBS, Illkirch, France ; 2015-University of Uppsala, Sweden, EMBO fellowship interview ; 2015-Norwich, UK ; 2014-MRC, UK ; 2013- University of South Carolina, USA ; 2013-LCB, Marseille, France.