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Béatrice Roche

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***EDUCATION*** **2015 Ph.D in microbiology**, University of Aix-Marseille, IMM, France. **2011 Postgraduate engineer**, Polytech School of Advanded Studies in Engineering of Luminy, Marseille, France. **2008 Bachelor's** degree in cellular biology, University of Aix-Marseille, France ***RESEARCH EXPERIENCES*** **2019-2022 Post-doctoral researcher**, University of Basel, Biozentrum; Infection Biology, Basel; Switzerland. Director: Pr Dirk BUMANN. **2016-2019 Post-doctoral researcher**, University of Strasbourg-CNRS, ESBS, UMR 7242, Illkirch, France. Director: Dr Isabelle SCHALK. **2011-2015 Ph.D** in microbiology, LCB, Marseille, France. Director: Pr Frédéric BARRAS. **2011** **Engineering internship**, Nosopharm Biotech company, Nîmes, France. ***MENTORING*** **2019-2020 Master**, Jasmin Künnecke, Biozentrum, Basel, Switzerland **2017-2019 Ph.D student**, Anne Bonneau, ESBS, Illkirch, France. MRT fellowship. **2017 Master**, Anne Bonneau, ESBS, Illkirch, France. **2017** **Training** in protein purification in liquid chromatography, CNRS-ESBS, Illkirch, France. ***GRANTS/FUNDINGS*** **2018 Instruct** (PID 6435), Macromolecular crystallization, 1500 € **2017 FRISBI** funding, Electron microscopy, 500 € **2017 Instruct** funding (PID 2886), Electron microscopy, 1500 € **2016-2019** **Hoffmann-La Roche** post-doctoral fellowship **2015** **Foundation for Medical Research (FRM)**, 4th year PhD salary **2011-2015** French Ministry of Advanced Instruction and Research, Ph.D fellowship ***MAJOR SKILLS*** **Writing: fundings and research articles (12)** including 6 first author and 3 corresponding author. **Review Editor** in Frontiers in Microbiology – Infectious Agents and Disease (from Nov 2021) **Molecular biology**: overlap PCR, gene cloning, construction of deletion mutant, PCR mutagenesis, RNA extraction. **Microbiology**: *E. coli* and BSL2 pathogens (*P. aeruginosa*, *S. enterica*), 55Fe uptake assay, conjugation, antibiotic killing. **Biochemistry**: protein production and purification (liquid chromatography), purification of membrane proteins, electrophoresis SDS-PAGE, immunodetection assays. **Biophysics/microscopy**: flow-cytometry, phase contrast and fluorescence microscopy, negative-stain EM data processing with Relion2, EMAN2.1, Chimera. **Animal skills**: mice handling, iv injection (FELASA number LTK4727) ***PUBLICATIONS*** 1\. **Roche B** \*, Garcia-Rivera MA, Normant V, Kuhn L, Hammann P, Brönstrup M, Mislin GLA, Schalk IJ \*. A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa. *Environ Microbiol.* **2021**. doi: 10.1111/1462-2920.15811 2\. **Roche B** \*, Mislin GLA and Schalk IJ. Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway. *FEBS Lett*. **2020**; 595: 370-378. 3\. **Roche B** and Bumann D \*. Single-cell reporters for pathogen responses to antimicrobial host attacks. *Curr Opin Microbiol*. **2020**; 59:16-23. 4\. Perraud Q, Cantero P, **Roche B**, Gasser V, Normant VP, Kuhn L, Hammann P, Mislin GLA, Ehret-Sabatier L and Schalk IJ \*. Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms. *Mol Cell Proteomics*. **2020**;19: 589–607. 5\. Bonneau A, **Roche B** \***,** Schalk IJ \*. Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins. *Sci Rep*. **2020**;10: 120. 6\. Paulen A, Hoegy F, **Roche B**, Schalk IJ, Mislin GLA \*. Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue. *Bioorg Med Chem Lett*. **2017**;27: 4867–4870. 7\. Ganne G, Brillet K, Basta B, **Roche B**, Hoegy F, Gasser V and Schalk IJ \*. Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH. *ACS Chem Biol*. **2017**;12: 1056–1065. 8\. **Roche B**, Agrebi R, Huguenot A, Ollagnier de Choudens S, Barras F, Py B \*. Turning Escherichia coli into a Frataxin-Dependent Organism. *PLoS Genet*. **2015**;11: e1005134. 9\. **Roche B**, Huguenot A, Barras F, Py B \*. The iron-binding CyaY and IscX proteins assist the ISC-catalyzed Fe-S biogenesis in Escherichia coli. *Mol Microbiol*. **2015**;95: 605–623. 10\. Yan R, Konarev PV, Iannuzzi C, Adinolfi S, **Roche B,** Kelly G, et al. Ferredoxin competes with bacterial frataxin in binding to the desulfurase IscS. *J Biol Chem*. **2013**;288: 24777–24787. 11\. **Roche B**, Aussel L, Ezraty B, Mandin P, Py B, Barras F \*. Iron/sulfur proteins biogenesis in prokaryotes: formation, regulation and diversity. *Biochim Biophys Acta*. **2013**;1827: 455–469. 12\. Bordi C, Lamy M-C, Ventre I, Termine E, Hachani A, Fillet S, **Roche B**, Bleves S, et al. Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis. *Mol Microbiol.* **2010**;76: 1427–1443. \* = corresponding author ***SCIENTIFIC COMMUNICATIONS*** **2022&2021**-Biozentrum, Basel, Switzerland ; **2020**-LBP, Illkirch, France ; **2020-**Biozentrum, Basel, Switzerland ; **2018**-Institut Pasteur, Paris, France ; **2017**-Hoffmann-la Roche, Basel, Switzerland ; **2017**-ESBS, Illkirch, France ; **2016**-ESBS, Illkirch, France ; **2015**-University of Uppsala, Sweden, EMBO fellowship interview ; **2015**-Norwich, UK ; **2014**-MRC, UK ; **2013**- University of South Carolina, USA ; **2013**-LCB, Marseille, France.

Publications

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Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway

Béatrice Roche , Gaëtan L.A. Mislin , Isabelle J Schalk
FEBS Letters, In press, ⟨10.1002/1873-3468.14012⟩
Journal articles hal-03039826v1
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A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa

Béatrice Roche , Mariel Garcia‐rivera , Vincent Normant , Lauriane Kuhn , Philippe Hammann et al.
Environmental Microbiology, 2021, ⟨10.1111/1462-2920.15811⟩
Journal articles hal-03404109v1
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Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins

Anne Bonneau , Béatrice Roche , Isabelle Schalk
Scientific Reports, 2020, 10 (1), ⟨10.1038/s41598-019-56913-x⟩
Journal articles hal-02982784v1
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Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms

Quentin Perraud , Paola Cantero , Béatrice Roche , Véronique Gasser , Vincent Normant et al.
Molecular and Cellular Proteomics, 2020, 19 (4), pp.589-607. ⟨10.1074/mcp.RA119.001829⟩
Journal articles hal-02555371v1
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Prospective evaluation of a dynamic insulin infusion algorithm for non critically-ill diabetic patients: A before-after study

Nathanaëlle Montanier , Lise Bernard , Celine Lambert , Bruno Pereira , Françoise Desbiez et al.
PLoS ONE, 2019, 14 (1), pp.e0211425. ⟨10.1371/journal.pone.0211425⟩
Journal articles hal-02194859v1
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Issues in amiodarone-induced thyrotoxicosis: Update and review of the literature

Salwan Maqdasy , Thomas Benichou , Sarah Dallel , Béatrice Roche , Françoise Desbiez et al.
Annales d'Endocrinologie, 2019, 80, pp.54 - 60. ⟨10.1016/j.ando.2018.05.001⟩
Journal articles hal-03486649v1

Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue

Aurélie Paulen , Françoise. Hoegy , Béatrice. Roche , Isabelle Schalk , Gaëtan L.A. Mislin et al.
Bioorganic and Medicinal Chemistry Letters, 2017, 27 (21), pp.4867-4870. ⟨10.1016/j.bmcl.2017.09.039⟩
Journal articles hal-02348563v1

Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH

Géraldine Ganne , Karl Brillet , Beata Szafarowicz Basta , Béatrice Roche , Françoise Hoegy et al.
ACS Chemical Biology, 2017, 12 (4), pp.1056-1065. ⟨10.1021/acschembio.6b01077⟩
Journal articles hal-02348567v1

Amiodarone-Induced Thyrotoxicosis Recurrence After Amiodarone Reintroduction.

Salwan Maqdasy , Marie Batisse-Lignier , Candy Auclair , Françoise Desbiez , Bernard Citron et al.
American Journal of Cardiology, 2016, 117 (7), pp.1112-6. ⟨10.1016/j.amjcard.2016.01.003⟩
Journal articles hal-03020397v1

The iron-binding CyaY and IscX proteins assist the ISC-catalyzed Fe-S biogenesis in Escherichia coli

Beatrice Roche , Allison Huguenot , Frederic Barras , Beatrice Py
Molecular Microbiology, 2015, 95 (4), pp.605--623. ⟨10.1111/mmi.12888⟩
Journal articles hal-01452060v1
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Turning Escherichia coli into a Frataxin-Dependent Organism

Béatrice Roche , Rym Agrebi , Allison Huguenot , Sandrine Ollagnier de Choudens , Frédéric Barras et al.
PLoS Genetics, 2015, 11 (5), pp.UNSP e1005134. ⟨10.1371/journal.pgen.1005134⟩
Journal articles hal-01213670v1
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Leydig cell tumor in a patient with 49,XXXXY karyotype: a review of literature

Salwan Maqdasy , Laura Bogenmann , Marie Batisse-Lignier , Béatrice Roche , Frédéric Franck et al.
Reproductive Biology and Endocrinology, 2015, 13, pp.72. ⟨10.1186/s12958-015-0071-7⟩
Journal articles hal-01913959v1

Ferredoxin Competes with Bacterial Frataxin in Binding to the Desulfurase IscS*

Robert Yan , Petr Konarev , Clara Iannuzzi , Salvatore Adinolfi , Béatrice Roche et al.
Journal of Biological Chemistry, 2013, 288 (34), pp.24777-24787. ⟨10.1074/jbc.M113.480327⟩
Journal articles hal-03616114v1

Reprint of: Iron/sulfur proteins biogenesis in prokaryotes: Formation, regulation and diversity

Béatrice Roche , Laurent Aussel , Benjamin Ezraty , Pierre Mandin , Béatrice Py et al.
Biochimica biophysica acta (BBA) - Bioenergetics, 2013, 1827 (8-9), pp.923-937. ⟨10.1016/j.bbabio.2013.05.001⟩
Journal articles hal-03616227v1