
Béatrice Roche
Présentation
EDUCATION
2015 Ph.D in microbiology, University of Aix-Marseille, IMM, France.
2011 Postgraduate engineer, Polytech School of Advanded Studies in Engineering of Luminy, Marseille, France.
2008 Bachelor's degree in cellular biology, University of Aix-Marseille, France
RESEARCH EXPERIENCES
2019-2022 Post-doctoral researcher, University of Basel, Biozentrum; Infection Biology, Basel; Switzerland. Director: Pr Dirk BUMANN.
2016-2019 Post-doctoral researcher, University of Strasbourg-CNRS, ESBS, UMR 7242, Illkirch, France. Director: Dr Isabelle SCHALK.
2011-2015 Ph.D in microbiology, LCB, Marseille, France. Director: Pr Frédéric BARRAS.
2011 Engineering internship, Nosopharm Biotech company, Nîmes, France.
MENTORING
2019-2020 Master, Jasmin Künnecke, Biozentrum, Basel, Switzerland
2017-2019 Ph.D student, Anne Bonneau, ESBS, Illkirch, France. MRT fellowship.
2017 Master, Anne Bonneau, ESBS, Illkirch, France.
2017 Training in protein purification in liquid chromatography, CNRS-ESBS, Illkirch, France.
GRANTS/FUNDINGS
2018 Instruct (PID 6435), Macromolecular crystallization, 1500 €
2017 FRISBI funding, Electron microscopy, 500 €
2017 Instruct funding (PID 2886), Electron microscopy, 1500 €
2016-2019 Hoffmann-La Roche post-doctoral fellowship
2015 Foundation for Medical Research (FRM), 4th year PhD salary
2011-2015 French Ministry of Advanced Instruction and Research, Ph.D fellowship
MAJOR SKILLS
Writing: fundings and research articles (12) including 6 first author and 3 corresponding author.
Review Editor in Frontiers in Microbiology – Infectious Agents and Disease (from Nov 2021)
Molecular biology: overlap PCR, gene cloning, construction of deletion mutant, PCR mutagenesis, RNA extraction.
Microbiology: E. coli and BSL2 pathogens (P. aeruginosa, S. enterica), 55Fe uptake assay, conjugation, antibiotic killing.
Biochemistry: protein production and purification (liquid chromatography), purification of membrane proteins, electrophoresis SDS-PAGE, immunodetection assays.
Biophysics/microscopy: flow-cytometry, phase contrast and fluorescence microscopy, negative-stain EM data processing with Relion2, EMAN2.1, Chimera.
Animal skills: mice handling, iv injection (FELASA number LTK4727)
PUBLICATIONS
1. Roche B *, Garcia-Rivera MA, Normant V, Kuhn L, Hammann P, Brönstrup M, Mislin GLA, Schalk IJ *. A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa. Environ Microbiol. 2021. doi: 10.1111/1462-2920.15811
2. Roche B *, Mislin GLA and Schalk IJ. Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway. FEBS Lett. 2020; 595: 370-378.
3. Roche B and Bumann D *. Single-cell reporters for pathogen responses to antimicrobial host attacks. Curr Opin Microbiol. 2020; 59:16-23.
4. Perraud Q, Cantero P, Roche B, Gasser V, Normant VP, Kuhn L, Hammann P, Mislin GLA, Ehret-Sabatier L and Schalk IJ *. Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms. Mol Cell Proteomics. 2020;19: 589–607.
5. Bonneau A, Roche B *, Schalk IJ *. Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins. Sci Rep. 2020;10: 120.
6. Paulen A, Hoegy F, Roche B, Schalk IJ, Mislin GLA *. Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue. Bioorg Med Chem Lett. 2017;27: 4867–4870.
7. Ganne G, Brillet K, Basta B, Roche B, Hoegy F, Gasser V and Schalk IJ *. Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH. ACS Chem Biol. 2017;12: 1056–1065.
8. Roche B, Agrebi R, Huguenot A, Ollagnier de Choudens S, Barras F, Py B *. Turning Escherichia coli into a Frataxin-Dependent Organism. PLoS Genet. 2015;11: e1005134.
9. Roche B, Huguenot A, Barras F, Py B *. The iron-binding CyaY and IscX proteins assist the ISC-catalyzed Fe-S biogenesis in Escherichia coli. Mol Microbiol. 2015;95: 605–623.
10. Yan R, Konarev PV, Iannuzzi C, Adinolfi S, Roche B, Kelly G, et al. Ferredoxin competes with bacterial frataxin in binding to the desulfurase IscS. J Biol Chem. 2013;288: 24777–24787.
11. Roche B, Aussel L, Ezraty B, Mandin P, Py B, Barras F *. Iron/sulfur proteins biogenesis in prokaryotes: formation, regulation and diversity. Biochim Biophys Acta. 2013;1827: 455–469.
12. Bordi C, Lamy M-C, Ventre I, Termine E, Hachani A, Fillet S, Roche B, Bleves S, et al. Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis. Mol Microbiol. 2010;76: 1427–1443.
* = corresponding author
SCIENTIFIC COMMUNICATIONS
2022&2021-Biozentrum, Basel, Switzerland ; 2020-LBP, Illkirch, France ; **2020-**Biozentrum, Basel, Switzerland ; 2018-Institut Pasteur, Paris, France ; 2017-Hoffmann-la Roche, Basel, Switzerland ; 2017-ESBS, Illkirch, France ; 2016-ESBS, Illkirch, France ; 2015-University of Uppsala, Sweden, EMBO fellowship interview ; 2015-Norwich, UK ; 2014-MRC, UK ; 2013- University of South Carolina, USA ; 2013-LCB, Marseille, France.