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**Equipe SeeDev, UMR 1318 Institut Jean Pierre Bourgin, INRA, centre de Versailles** From my past and present research achievements, I have acquired a good expertise in plant molecular genetics. My technical skills are based on the tools of molecular biology, cytology and bioinformatics and serve to answer biological questions involving seed development. My main topic is the transcriptional network controlling the switch between embryogenesis and accumulation of reserves. I am focusing on LEC2, a master regulator of seed maturation belonging to the B3 family. My work aims to determine (i) how this factor binds, or interact, with its DNA targets, in a possible complex with ABI3 and FUS3, (ii) how is this protein complex is regulated, and (iii) how LEC2 is transcriptionnaly and post-transcriptionally tuned. We aim at modelling the regulatory network involved in Arabidopsis and investigate how it is conserved in cereals. Scientific manager of the cytology and imaging platform of the Plant Observatory, I ensure the proper scientific and human functioning of a strategic tool for my institute (1200M2, 5 Full Time Equivalents, 100 regular users, 5 confocal micoscopes, laser microdissector, twenty large optic pieces, and various cytology-related materials Operating correspondent for the institute, I manage all aspects of the unit's 9 buildings (8000M2), requests for equipment, works, modifications, in relations with the research center for all these aspects. I am also managing the unit’s workshop (3 FTEs).


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Custom methods to identify conserved genetic modules applied to novel transcriptomic data from Amborella trichopoda

Ana Rivarola Sena , Amélie Andres-Robin , Aurélie Vialette , Jérémy Just , Alexandra Launay-Avon et al.
Journal of Experimental Botany, 2022, 73 (8), pp.2487-2498. ⟨10.1093/jxb/erac044⟩
Journal articles hal-03566722v1
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The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

Pierre Jacob , Gwilherm Brisou , Marion Dalmais , Johanne Thévenin , Froukje van Der Wal et al.
Genes, 2021, 12 (5), pp.1-18. ⟨10.3390/genes12050746⟩
Journal articles hal-03335244v1

Genetic and Molecular Control of Somatic Embryogenesis

Camille Salaün , Loïc Lepiniec , Bertrand Dubreucq
Plants, 2021, 10 (7), pp.1467. ⟨10.3390/plants10071467⟩
Journal articles hal-03754308v1

Variation in expression of the HECT E3 ligase UPL3 modulates LEC2 levels, seed size, and crop yields in brassica napus

Charlotte Miller , Rachel Wells , Neil Mckenzie , Martin Trick , Joshua Ball et al.
The Plant cell, 2019, 31 (10), pp.2370-2385. ⟨10.1105/tpc.18.00577⟩
Journal articles hal-02622699v1

Regulation of FUSCA3 Expression During Seed Development in Arabidopsis

Thomas James Roscoe , Virginie Vaissayre , Gaël Paszkiewicz , Fernando Clavijo , Zsolt Kelemen et al.
Plant and Cell Physiology, 2019, 60 (2), pp.476-487. ⟨10.1093/pcp/pcy224⟩
Journal articles hal-02386031v1
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Combining laser-assisted microdissection (LAM) and RNA-seq allows to perform a comprehensive transcriptomic analysis of epidermal cells of Arabidopsis embryo

Kaori Sakai , Ludivine Taconnat , Néro Borrega , Jennifer Yansouni , Véronique Brunaud et al.
Plant Methods, 2018, 14, pp.10. ⟨10.1186/s13007-018-0275-x⟩
Journal articles hal-02625394v1

LEC1 (NF-YB9) directly interacts with LEC2 to control gene expression in seed

Céline Boulard , Johanne Thévenin , O. Tranquet , V. Laporte , L. Lepiniec et al.
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , 2018, 1861 (5), pp.443-450. ⟨10.1016/j.bbagrm.2018.03.005⟩
Journal articles hal-01746034v1
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Molecular and epigenetic regulations and functions of the LAFL transcriptional regulators that control seed development

L. Lepiniec , M. Devic , T. Roscoe , D. Bouyer , D.-X Zhou et al.
Sexual Plant Reproduction, 2018, 31 (3), pp.291-307. ⟨10.1007/s00497-018-0337-2⟩
Journal articles hal-01969458v1

Regulation and evolution of the interaction of the seed B3 transcription factors with NF-Y subunits

Céline Boulard , Abdelhak Fatihi , Loic Lepiniec , Bertrand Dubreucq
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , 2017, 1860 (10), pp.1069-1078. ⟨10.1016/j.bbagrm.2017.08.008⟩
Journal articles hal-02627256v1

Deciphering and modifying LAFL transcriptional regulatory network in seed for improving yield and quality of storage compounds

Sébastien Baud , Abdelhak Fatihi , Celine Boulard , Daniel Bouyer , Sebastien S. Baud et al.
Plant Science, 2016, 250, pp.198-204. ⟨10.1016/j.plantsci.2016.06.013⟩
Journal articles hal-02356216v1

The Physcomitrella patens System for Transient Gene Expression Assays.

Johanne Thévenin , Wenjia Xu , Louise Vaisman , Loic Lepiniec , Bertrand Dubreucq et al.
Methods in Molecular Biology, 2016, 1482, pp.151-61. ⟨10.1007/978-1-4939-6396-6_10⟩
Journal articles hal-01389059v1

Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by L-AFL proteins in Arabidopsis seed.

Sebastien S. Baud , Zsolt Kelemen , Johanne Thévenin , Céline Boulard , Sandrine Blanchet et al.
Plant Physiology, 2016, 171 (171), pp.1099-1112. ⟨10.1104/pp.16.00034⟩
Journal articles hal-01406124v1
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La caméline, une plante modèle pour la recherche translationnelle

Mark Tepfer , Bertrand Dubreucq , Jean-Denis Faure
Biofutur, 2016, 380, pp.38-41
Journal articles hal-01529235v1

The structural organization of seed oil bodies could explain the contrasted oil extractability observed in two rapeseed genotypes.

Celine Boulard , Michel Bardet , Thierry Chardot , Bertrand Dubreucq , Marina Gromova et al.
Planta, 2015, 242 (1), pp.53-68. ⟨10.1007/s00425-015-2286-4⟩
Journal articles hal-01204182v1
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Imaging and quantitative analysis of size and distribution of sperical bodies, [i]e.g[/i]. embryonic oil bodies

Martine Miquel , Ghassen Trigui , Alain Trubuil , Bertrand Dubreucq
Bio-protocol , 2015, 5 (1), pp.1-6
Journal articles hal-01204094v1
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Analysis of the DNA-Binding Activities of the Arabidopsis R2R3-MYB Transcription Factor Family by One-Hybrid Experiments in Yeast

Zsolt Kelemen , Alvaro Sebastian , Wenjia Xu , Damaris Grain , Fabien Salsac et al.
PLoS ONE, 2015, 10 (10), pp.e0141044. ⟨10.1371/journal.pone.0141044⟩
Journal articles hal-01837546v1

Role of B3 domain transcription factors of the AFL family in maize kernel filling

Aurélie Grimault , Ghislaine Gendrot , Sandrine Chaignon , Françoise Gilard , Guillaume Tcherkez et al.
Plant Science, 2015, 236, pp.116-125. ⟨10.1016/j.plantsci.2015.03.021⟩
Journal articles hal-01204180v1

ZmZHOUPI, an endosperm-specific basic helix-loop-helix transcription factor involved in maize seed development

Aurélie Grimault , Ghislaine Gendrot , Sophy Chamot , Thomas Widiez , Hervé Rabillé et al.
Plant Journal, 2015, 84 (3), pp.574-586. ⟨10.1111/tpj.13024⟩
Journal articles hal-01536562v1

Specialization of oleosins in OB dynamics during seed development in Arabidopsis thaliana seeds.

Martine Miquel , Ghassen Trigui , Sabine d'Andrea , Zsolt Kelemen , Sebastien S. Baud et al.
Plant Physiology, 2014, 164 (4), pp.1866-1878. ⟨10.1104/pp.113.233262⟩
Journal articles hal-01019614v1
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Function and localization of the Arabidopsis thaliana diacylglycerol acyltransferase DGAT2 expressed in yeast

Laure Aymé , Sebastien S. Baud , Bertrand Dubreucq , Florent Joffre , Thierry Chardot et al.
PLoS ONE, 2014, 9 (3), 9 p. ⟨10.1371/journal.pone.0092237⟩
Journal articles hal-01204016v1

MYB118 Represses Endosperm Maturation in Seeds of Arabidopsis

Guillaume Barthole , Alexandra To , Chloé Marchive , Veronique Brunaud , Ludivine Soubigou-Taconnat et al.
The Plant cell, 2014, 26 (9), pp.3519 - 3537. ⟨10.1105/tpc.114.130021⟩
Journal articles hal-01204153v1

Endosperm breakdown in Arabidopsis requires heterodimers of the basic helix-loop-helix proteins ZHOUPI and INDUCER OF CBP EXPRESSION 1

Gregoire Denay , Audrey Creff , Steven Moussu , Pauline Wagnon , Johanne Thévenin et al.
Development (Cambridge, England), 2014, 141 (6), pp.1222-U85. ⟨10.1242/dev.103531⟩
Journal articles hal-01204028v1
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Expression variation in connected recombinant populations of Arabidopsis thaliana highlights distinct transcriptome architectures

Francisco Cubillos , Jennifer Yansouni , Ahmid Khalili , Sandrine Balzergue , Samira Elftieh et al.
BMC Genomics, 2012, 13, pp.1-12. ⟨10.1186/1471-2164-13-117⟩
Journal articles hal-01189696v1
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The dynamics of oil bodies in Arabidopsis thaliana seeds : a mathematical model of biogenesis and coalescence

Ghassen Trigui , Béatrice Laroche , Martine Miquel , Bertrand B. Dubreucq , Alain A. Trubuil et al.
International Journal of Electrical, Computer, Energetic, Electronic and Communication Engineering, 2012, 64, pp.763-768
Journal articles hal-02643123v1

A comprehensive overview of grain development in Brachypodium distachyon variety Bd21

Fabienne Guillon , Colette Larre , Fabrice Petitpas , Adeline Berger , Jihad Moussawi et al.
Journal of Experimental Botany, 2012, 63 (2), pp.739-755. ⟨10.1093/jxb/err298⟩
Journal articles hal-01190419v1

Evolution goes GAGA: GAGA binding proteins across kingdoms

Nathalie N. Berger , Bertrand B. Dubreucq
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , 2012, 1819 (8), pp.863 - 868. ⟨10.1016/j.bbagrm.2012.02.022⟩
Journal articles hal-01004142v1

A new system for fast and quantitative analysis of heterologous gene expression in plants

Johanne Thévenin , Christian Dubos , Wenjia Xu , José Gentilhomme-Le Gourrierec , Zolt Kelemen et al.
New Phytologist, 2012, 193 (2), pp.504 - 512. ⟨10.1111/j.1469-8137.2011.03936.x⟩
Journal articles hal-01004263v1
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Transcriptional regulation of Arabidopsis LEAFY COTYLEDON2 involves RLE,a cis-element that regulates Trimethylation of Histone H3 at Lysine-27

Nathalie N. Berger , Bertrand B. Dubreucq , François Roudier , Christian C. Dubos , Loic L. Lepiniec et al.
The Plant cell, 2011, 23 (11), pp.4065-4078. ⟨10.1105/tpc.111.087866⟩
Journal articles hal-01000017v1
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NOF1 encodes an arabidopsis protein involved in the control of rRNA expression

Erwana Harscoet , Bertrand Dubreucq , Jean-Christophe Palauqui , Loic Lepiniec
PLoS ONE, 2010, 5 (9), pp.e12829. ⟨10.1371/journal.pone.0012829⟩
Journal articles hal-01203890v1
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Arabidopsis seed secrets unravelled after a decade of genetic and omics-driven research

Helen North , Sebastien S. Baud , Isabelle Debeaujon , Christian Dubos , Bertrand Dubreucq et al.
Plant Journal, 2010, 61 (6), pp.971-981. ⟨10.1111/j.1365-313X.2009.04095.x⟩
Journal articles hal-01203892v1

Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis.

Monica Santos-Mendoza , Bertrand Dubreucq , Sebastien S. Baud , François Parcy , Michel Caboche et al.
Plant Journal, 2008, 54 (4), pp.608-20. ⟨10.1111/j.1365-313X.2008.03461.x⟩
Journal articles hal-00297574v1

High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis.

Elisabeth Truernit , Hélène Bauby , Bertrand Dubreucq , Olivier Grandjean , John Runions et al.
The Plant cell, 2008, 20 (6), pp.1494-1503. ⟨10.1105/tpc.107.056069⟩
Journal articles hal-02663189v1

WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis

Sebastien S. Baud , Monica Santos Mendoza , Alexandra A. To , Erwana E. Harscoet , Loic L. Lepiniec et al.
Plant Journal, 2007, 50 (5), pp.825-838. ⟨10.1111/j.1365-313X.2007.03092.x⟩
Journal articles hal-02664890v1

Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana

Sebastien S. Baud , Sylvie Wuilleme , Bertrand B. Dubreucq , Aurélie de Almeida , Cindy Vuagnat et al.
Plant Journal, 2007, 52 (3), pp.405-419. ⟨10.1111/j.1365-313X.2007.03232.x⟩
Journal articles hal-02661879v1

LEAFY COTYLEDON 2 activation is sufficient to trigger the accumulation of oil and seed specific mRNAs in Arabidopsis leaves

Monica Santos-Mendoza , Bertrand B. Dubreucq , Martine Miquel , Michel M. Caboche , Loic L. Lepiniec et al.
FEBS Letters, 2005, 579 (21), pp.4666-4670. ⟨10.1016/j.febslet.2005.07.037⟩
Journal articles hal-02682706v1

TT2, TT8 and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis

A. Baudry , M.A. Heim , Bertrand B. Dubreucq , B. Weisshaar , Loic L. Lepiniec et al.
Plant Journal, 2004, 39 (3), pp.366-380. ⟨10.1111/j.1365-313X.2004.02138.x⟩
Journal articles hal-02683484v1
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The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects

Esther Lechner , Daoxin Xie , Sandrine Grava , Emmanuelle Pigaglio , Séverine Planchais et al.
Journal of Biological Chemistry, 2002, 277 (51), pp.50069-50080. ⟨10.1074/jbc.M204254200⟩
Journal articles hal-02682424v1

T-DNA integration into Arabidopsis genome depends on sequences of pre-insertion sites

Véronique Brunaud , Sandrine Balzergue , Bertrand B. Dubreucq , Sebastien Aubourg , Franck Samson et al.
EMBO Reports, 2002, 3 (12), pp.1152-1157. ⟨10.1093/embo-reports/kvf237⟩
Journal articles hal-02682810v1
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FLAGdb/FST: a database of mapped flanking insersion sites (FSTs) of Arabidopsis thaliana T-DNA transformants

Franck Samson , Véronique Brunaud , Sandrine Balzergue , Bertrand B. Dubreucq , Loic L. Lepiniec et al.
Nucleic Acids Research, 2002, 30 (1), pp.94-97
Journal articles hal-02678939v1

Approches moléculaires de la qualité et du développement des graines

Bertrand B. Dubreucq , Philippe Grappin , Martine Miquel , Helen H. North , Christine Rochat et al.
Oléagineux, Corps Gras, Lipides, 2001, 8 (5), pp.487-495
Journal articles hal-02674355v1

Arabidopsis glucosidase I mutants reveal a critical role of N-glycan trimming in seed development

M. Boisson , Veronique Gomord , Corinne Audran , Nathalie Berger , Bertrand B. Dubreucq et al.
EMBO Journal, 2001, 20 (5), pp.1010-1019
Journal articles hal-02683628v1

Improved PCR-walking for large-scale isolation of plant T-DNA borders

Sandrine Balzergue , Bertrand B. Dubreucq , Sylvie Chauvin , I. Le-Clainche , F. Le Boulaire et al.
Biotechniques, 2001, 30 (3), 3 p
Journal articles hal-02670631v1

The Arabidopsis AtPRP1 extensin-like gene is specifically expressed in endosperm during seed germination

Bertrand B. Dubreucq , Nathalie Berger , E. Vincent , M. Boisson , G. Pelletier et al.
Plant Journal, 2000, 5, pp.643-652
Journal articles hal-02688788v1

La germination vient en dormant

Michel M. Caboche , Bertrand B. Dubreucq , Philippe Grappin , P. Lepiniec , Nathalie Nesi et al.
Biofutur, 1998, 175, pp.32-35
Journal articles hal-02689896v1

A new method for the identification and isolation of genes essential for Arabidopsis thaliana seed germination

Bertrand B. Dubreucq , Philippe Grappin , Michel M. Caboche
Molecular and General Genetics MGG, 1996, 252, pp.42-50
Journal articles hal-02692047v1

Shared Task SeeDev : Extraction de régulations impliquées dans le développement de la graine d’Arabidopsis thaliana à partir de publications scientifiques

Estelle Chaix , Bertrand Dubreucq , Dialekti Valsamou , Abdelhak Fatihi , Louise Deleger et al.
Les journées Bioinformatique de l'Inra, Mar 2016, Montpellier, France. pp.1
Conference papers hal-01455855v1
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Overview of the regulatory network of plant seed development (SeeDev) task at the BioNLP shared task 2016

Estelle Chaix , Bertrand Dubreucq , Abdelhak Fatihi , Dialekti Valsamou , Robert Bossy et al.
BioNLP Shared Task, Aug 2016, Berlin, Germany. pp.113
Conference papers hal-01455854v1

Information extraction from articles for the elaboration of the regulatory networks involved in Arabidopsis seed development

Bertrand Dubreucq , Dialekti Valsamou , Abdelhak Fatihi , Estelle Chaix , Robert Bossy et al.
26th International Conference on Arabidopsis Research, Jul 2015, Paris, France
Conference papers hal-01524850v1

Information extraction challenge Gene Regulation Network in Arabidopsis thaliana (GRNA)

Estelle Chaix , Bertrand Dubreucq , Dialekti Valsamou , Abdelhak Fatihi , Robert Bossy et al.
Paris-Saclay Center for Data Science: Open Software Initiative (OSI), Oct 2015, Orsay, France. pp.14 slides, ⟨10.13140/RG.2.1.4286.6326⟩
Conference papers hal-01603453v1
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Knowledge model of regulatory networks involved in Arabidopsis seed development for information extraction and integration from text

Estelle Chaix , Bertrand Dubreucq , Dialekti Valsamou , Abdelhak Fatihi , Robert Bossy et al.
BioCreative 5, Sep 2015, Madrid, Spain
Conference papers hal-01512197v1
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Analysis of factors affecting the growth of oil bodies in A. Thaliana seeds: use of ordinary least squares and quantile regression

Ghassen Trigui , Martine Miquel , Bertrand B. Dubreucq , Olivier David , Alain A. Trubuil et al.
The 10th International Workshop on Computational Systems Biology, WCSB 2013, Jun 2013, Tampere, Finland. pp.144
Conference papers hal-02748214v1
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The dynamics of oil bodies in A. thaliana seeds :A mathematical model of biogenesis and coalescence

Ghassen Trigui , Béatrice Laroche , Martine Miquel , Bertrand Dubreucq , Alain Trubuil et al.
ICMB 2012 - VIII International Conference on Mathematical Biology, Labo/service de l'auteur, Ville service., Apr 2012, Paris, France. pp.763-768
Conference papers hal-01019421v1

Brachypodium distachyon grain: development-associated changes in morphology and storage accumulation

Colette Larre , Anne-Laure Chateigner Boutin , Brigitte Bouchet , Fabrice Petitpas , Hélène Rogniaux et al.
1. European Brachypodium Workshop, Oct 2011, Versailles, France
Conference papers hal-02809921v1

L'alimentation des vaches est susceptible de modifier les caractéristiques sensorielles du lait

Bertrand B. Dubreucq , B. Martin , Anne Ferlay , Philippe Pradel , Isabelle Verdier-Metz et al.
9. Rencontres Recherches Ruminants, Dec 2002, Paris, France
Conference papers hal-02761189v1

Isolation and characterization of Arabidopsis mutants affected in seed development or germination

Loic L. Lepiniec , Sandrine Balzergue , Nicole Bechtold , M. Boisson , Bertrand B. Dubreucq et al.
9. International conference on Arabidopsis research, Jun 1998, Madison, United States
Conference papers hal-02765356v1

Seed Development

Bertrand Dubreucq , Sebastien S. Baud , Isabelle Debeaujon , Christian Dubos , Annie Marion-Poll et al.
Plant Developmental Biology - Biotechnological Perspectives, Springer-verlag, 497 p., 2010, 978-3-642-02300-2. ⟨10.1007/978-3-642-02301-9_17⟩
Book sections hal-01203968v1