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Fernanda FONSECA

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#### **EDUCATION** **Habilitation à Diriger des Recherches (HDR).** Université de Technologie de Compiègne, 2010. Title : « Stabilization of bio functionalities trough an approach combining the understanding of mechanisms and the product-process engineering” **Ph.D. Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech),**2001**. Ph.D. thesis title: "Influence of cryoprotection and freezing on the acidification activity of thermopile lactic acid bacteria: relationships between biological and thermodynamic properties." **Master Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech), **1997**. M.Sc. thesis title: "Study of the freezing step on the activity of frozen lactic acid starters." Engineer** in **Chemical Engineering**, Montevideo University (Uruguay), **1996.** **SCIENTIFIC EXPERIENCE** ------------------------- **Research Director (since January 2015),** in Paris-Saclay Food and Bioproduct Engineering research unit (UMR 782 SayFood INRAE-AgroParisTech), Versailles-Grignon Centre, at the French National Institute for Agriculture, Food and Environment (INRAE), France. **Researcher Scientist** (**September 2001 – December 2014**), in the Joint Research Unit for Food Process Engineering and Microbiology (UMR 782 GMPA INRA-AgroParisTech), Versailles-Grignon Centre, Institut National de la Recherche Agronomique (INRA), France. **Main fields of expertise:**Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. - Bioproducts: micro-organisms, proteins, food products. - Stabilization processes: freezing and freeze-drying - Biophysical characterization of bioproducts (thermal analysis, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy) **Research approach:** Focusing on biophysical and chemical behaviors of bioproducts during stabilization processes and by relating them to biological and functional responses, to identify and quantify the mechanisms governing the degradation and preservation of bioproducts during the stabilization processes. The ambition is also to find general principles for simplifying and rationalizing the development of newly formulated products and the stabilization process optimization, with particular attention to limiting the environmental impact.

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712996
"stephanie-passot"
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Multi-objective optimization of frozen and freeze-dried Lactobacillus delbrueckii subsp. bulgaricus CFL1 production via the modification of fermentation conditions

Maria de Lourdes Tovilla Coutino , Stephanie Passot , Ioan-Cristian Trelea , Marie-Hélène Ropers , Yann Gohon
Journal of Applied Microbiology, 2023, 134 (2), pp.1-34. ⟨10.1093/jambio/lxad003⟩
Article dans une revue hal-04345553v1
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Effect of protective agents on the storage stability of freeze‑dried Ligilactobacillus salivarius CECT5713

Maria Guerrero Sanchez , Stéphanie Passot , Sonia Campoy , Monica Olivares , Fernanda Fonseca
Applied Microbiology and Biotechnology, 2022, 106, pp.7235-7249. ⟨10.1007/s00253-022-12201-9⟩
Article dans une revue hal-03845922v1
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Insights into lactic acid bacteria cryoresistance using FTIR micro-spectroscopy

Amélie Girardeau , Stephanie Passot , Julie Meneghel , Stéphanie Cenard , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2022, 414 (3), pp.1425-1443. ⟨10.1007/s00216-021-03774-x⟩
Article dans une revue hal-03552585v1
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Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

M. Guerrero Sanchez , Stephanie Passot , S. Campoy , M. Olivares , F. Fonseca
Applied Microbiology and Biotechnology, 2022, 106 (1), pp.57-80. ⟨10.1007/s00253-021-11694-0⟩
Article dans une revue hal-03822336v1
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Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Fernanda Fonseca , Caroline Pénicaud , E. Elizabeth Tymczyszyn , Andrea Gómez-Zavaglia , Stephanie Passot
Applied Microbiology and Biotechnology, 2019, 103 (17), pp.6867-6883. ⟨10.1007/s00253-019-10002-1⟩
Article dans une revue hal-03824544v1
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Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties

Fernanda Fonseca , Amélie Girardeau , Stephanie Passot
Willem F. Wolkers; Harriëtte Oldenhof. Cryopreservation and Freeze-Drying Protocols, 2180, Springer US, pp.703-719, 2021, Methods in Molecular Biology, 978-1-0716-0782-4. ⟨10.1007/978-1-0716-0783-1_38⟩
Chapitre d'ouvrage hal-03824400v1