Frédéric Cassé

### **CURRENT POSITION** ###### **Research engineer in Biology**, University of Caen, UNICAEN, France. > > Plateform Normandie Equine Vallée. > Team: UR 7450 BIOTARGEN Biology, genetics and osteoarticular and respiratory therapies. ### **ACADEMIC QUALIFICATIONS** ###### **Ph.D**. **in Molecular and Cellular Biology**, Sept 2008 - Oct 2011 > University of Caen, France awarded October 12th, 2011. > ###### **M.Sc. Molecular and cellular Biology,** Sept 2006 - June 2008 > Life sciences and health, specialty cell biology, University of Caen, France. > ###### **Bachelor's degree**, Sept 2005 - June 2006 > Life sciences, Science and technology, University of Caen, France. ### **RESEARCH EXPERIENCE** ###### **Research engineer in Biology**, Dec 2019 - present Plateform Normandie Equine Vallée. University of Caen, UNICAEN, France. Team: UR 7450 BIOTARGEN Biology, genetics and osteoarticular and respiratory therapies. Director: Dr Magali Demoor, co-director : Dr Nicolas Gruchy. Project: *Cellular therapy and tissue engineering for articular cartilage repair.* ###### **Research scientist**, Apr 2017 - Nov 2019 > Center for psychiatric neurosciences. Lausanne University Hospital, Switzerland. > Team: Adult hippocampal neurogenesis: from regulation mechanisms to functional impact. PI: Pr Nicolas Toni. > Project: *Roles of astrocytes in adult hippocampal neurogenesis and mood disorder.* ###### **Post-Doctoral position**, Mar 2015 - Mar 2017 Department of Fundamental Neurosciences. University of Lausanne, Switzerland. Team: Synaptic integration of new neurons in the adult hippocampus. PI: Pr Nicolas Toni. Project: *Control of adult hippocampal neurogenesis by astrocytes.* ###### **Post-Doctoral position,** Mar 2012 - Feb 2015 Institute of Molecular and Cellular Pharmacology. University of Nice, France. Team: Sumoylation in neuronal function and dysfunction. PI: Dr Stéphane Martin. Project: *Regulation of the sumoylation machinery in neuronal and glial cells.* ###### **Ph.D**. **training,** in Molecular and Cellular Biology, Sept 2008 - Oct 2011 University of Caen, France. Team: Serine Proteases and Pathophysiology of the neurovascular Unit. Supervisors: Pr Denis Vivien and Dr Fabian Docagne. Thesis title: Tissue type plasminogen activator: a new gliotransmitter of tripartite glutamatergic synapse. Defended October 12th, 2011. ### **TECHNICAL SKILLS** **Cellular Biology** Isolation and culture of equine and human articular chondrocytes, mesenchymal stem cell derived from bone marrow and umbilical cord blood (Equine). 3D cartilage organoid models. Isolation and culture of adult neuronal stem cell (rodent), primary cultures of rodent’s hippocampal and cortical neurons, astrocytes and mixed cultures of neurons and astrocytes, adherent mammalian cell lines (COS-7, HEK293T, CHO), fibroblast cell culture. Transfection and preparation of viruses (sindbis virus, lentivirus, moloney murine leukemia virus). Models of cell death. Test of cytotoxicity and apoptosis, proliferation and wound-healing assay. **Microbiology** Isolation and culture, bacterial transformation, plasmid amplification, gram coloration, API identification. **Molecular Biology** RNA and DNA extraction from cell culture and tissue, real-time quantitative PCR, transient transfection, siRNA transfection, plasmid construction and subcloning, site-specific mutagenesis, genotyping. **Biochemistry** SDS-Page, western blotting, dot-blot, immunoprecipitation, zymography assay, sumoylation assay, synaptosomal preparation, protein purification techniques. **Live cell Imaging** Fluorescence recovery after photobleaching (FRAP)/Fluorescence loss in photobleaching (FLIP) experiments, Dendra photoconversion, cell surface protein mobility trafficking with Super Ecliptic Phluorin, protein mobility trafficking with photoswitchable fluorescent protein Dendra2, calcium imaging, mitochondria imaging (Mito Tracker, Mito Timer), cell proliferation assay. **Microscopy** Immunohistochemistry, immunocytochemistry, acquisition and analyses of confocal images. **Rodent Surgery** Stereotactic surgery, intraperitoneal, intravenous, and intracerebral injections. **Behavioral tests** Morris water maze, object recognition and location, fear conditioning, place recognition. **Technical Software** Image J (Analysis and macro programming),Imaris,Volocity, zen, NIS, Snapgene, Scalfold proteome software, Funrich, GraphPad Prism,JMP, Statview, Photoshop, Canvas, Microsoft office.