Frédéric Pecorari

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Publications

Recombinant Human IgG1 Enhances Complement-Mediated Bacteriolysis and Macrophage Phagocytosis of Pseudomonas aeruginosa via Targeting Cell Surface Polysaccharides

Thae Thae Min , Audrey Guilbaud , Frédéric Pecorari , Pawana Panomket , Nitima Suttipanta et al.

ACS Infectious Diseases, 2026, Online ahead of print. ⟨10.1021/acsinfecdis.5c01032⟩

Article dans une revue inserm-05479887v1
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Development of potent Affitin-based bispecific NK cell engagers for the therapy of MSLN-expressing cancers

Tina Briolay , Tacien Petithomme , Hermelyne Gravoueille , Judith Fresquet , Sylvia Lambot et al.

Molecular Therapy: Oncology, 2025, pp.201095. ⟨10.1016/J.omton.2025.201095⟩

Article dans une revue inserm-05381179v1
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Optimized pipeline for generating highly potent neutralizing Affitins: Application to SARS-CoV-2 spike protein

Thomas Maurice , Mélanie Laporte , Nicolas Barquilla , Agnès Fortun , Klara Echasserieau et al.

International Journal of Biological Macromolecules, 2025, pp.149321. ⟨10.1016/j.ijbiomac.2025.149321⟩

Article dans une revue inserm-05392675v2

Construction of Synthetic VHH Libraries in Ribosome Display Format

Audrey Guilbaud , Frédéric Pecorari

Methods in Molecular Biology, 2023, Methods in Molecular Biology, 2681, pp.19-31. ⟨10.1007/978-1-0716-3279-6_2⟩

Article dans une revue inserm-04163220v1
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Antibacterial activity of human natural killer cells in the absence of accessory cells against extracellular<i>Staphylococcus aureus</i>and hypervirulent<i>Pseudomonas aeruginosa</i>bacteria

Audrey Guilbaud , Baptiste Lottin , Gwenann Cadiou , Tiffany Beauvais , Sylvia Lambot et al. Article dans une revue inserm-04788349v1
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A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening

Juliette Vaurs , Gaël Douchin , Klara Echasserieau , Romain Oger , Nicolas Jouand et al.

Scientific Reports, 2021, 11 (1), pp.17234. ⟨10.1038/s41598-021-96560-9⟩

Article dans une revue inserm-03331942v1
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Characterization of Affitin proteolytic digestion in biorelevant media and improvement of their stabilities via protein engineering

Aurélie Loussouarn , Ghislaine Béhar , Frédéric Pecorari , Mikael Croyal , Axelle Renodon-Cornière

Scientific Reports, 2020, 10 (1), pp.1-13. ⟨10.1038/s41598-020-76855-z⟩

Article dans une revue inserm-03007124v1
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Whole-bacterium ribosome display selection for isolation of highly specific anti-Staphyloccocus aureus Affitins for detection- and capture- based biomedical applications

Ghislaine Béhar , Axelle Renodon-Cornière , Stanimir Kambarev , Petar Vukojicic , Nathalie Caroff et al.

Biotechnology and Bioengineering, 2019, Epub ahead of print. ⟨10.1002/bit.26989⟩

Article dans une revue inserm-02140227v1
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Multivalent Affidendrons with High Affinity and Specificity toward Staphylococcus aureus as Versatile Tools for Modulating Multicellular Behaviors

Petar Vukojicic , Ghislaine Behar , Maun H. Tawara , Marcos Fernandez-Villamarin , Frédéric Pecorari et al.

ACS Applied Materials & Interfaces, 2019, 11 (24), pp.21391-21398. ⟨10.1021/acsami.9b05702⟩

Article dans une revue inserm-02167902v1
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Prosthetic groups for radioiodination and astatination of peptides and proteins: a comparative study of five potential bioorthogonal labeling strategies

Laurent Navarro , Marion Berdal , Michel Chérel , Frédéric Pecorari , Jean-François Gestin et al.

Bioorganic and Medicinal Chemistry, In press, pp.167-174. ⟨10.1016/j.bmc.2018.11.034⟩

Article dans une revue inserm-01939636v1
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Model Affitin and PEG modifications onto siRNA lipid nanocapsules: cell uptake and in vivo biodistribution improvements

Pauline Resnier , Elise Lepeltier , Anthea Lucrezia Emina , Natacha Galopin , Jérôme Bejaud et al.

RSC Advances, 2019, 9 (47), pp.27264-27278. ⟨10.1039/c9ra03668g⟩

Article dans une revue inserm-02337070v1
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Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus

Stanimir Kambarev , Frédéric Pecorari , Stéphane Corvec

Journal of Antimicrobial Chemotherapy, 2018, 73 (5), pp.1201 - 1205. ⟨10.1093/jac/dky016⟩

Article dans une revue inserm-01813206v1
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A novel, smaller scaffold for Affitins: Showcase with binders specific for EpCAM.

Valentina Kalichuk , Axelle Renodon-Cornière , Ghislaine Béhar , Federico Carrión , Gonzalo Obal et al.

Biotechnology and Bioengineering, 2017, Epub ahead of print. ⟨10.1002/bit.26463⟩

Article dans une revue inserm-01629637v1
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Draft Genome Sequence of Mycobacterium ulcerans S4018 Isolated from a Patient with an Active Buruli Ulcer in Benin, Africa

Stanimir Kambarev , Stéphane Corvec , Annick Chauty , Estelle Marion , Laurent Marsollier et al.

Genome Announcements, 2017, 5 (17), ⟨10.1128/genomeA.00248-17⟩

Article dans une revue inserm-01550042v1
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In vitro emergence of fluoroquinolone resistance in Cutibacterium (formerly Propionibacterium ) acnes and molecular characterization of mutations in the gyrA gene

Eve-Marie Takoudju , Aurélie Guillouzouic , Stanimir Kambarev , Frederic Pecorari , Stephane Corvec

Anaerobe, 2017, 47, pp.194 - 200. ⟨10.1016/j.anaerobe.2017.06.005⟩

Article dans une revue inserm-01636129v1
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Draft Genome Sequences of Two Highly Erythromycin-Resistant Streptococcus gallolyticus subsp. gallolyticus Isolates Containing a Novel Tn916-Like Element, Tn6331

Stanimir Kambarev , Frédéric Pecorari , Stéphane Corvec

Genome Announcements, 2017, 5 (16), Epub ahead of print. ⟨10.1128/genomeA.00226-17⟩

Article dans une revue inserm-01550034v1
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The archaeal "7kDa DNA-binding" proteins: extended characterization of an old gifted family

Valentina Kalichuk , Ghislaine Béhar , Axelle Renodon-Cornière , Georgi Danovski , Gonzalo Obal et al.

Scientific Reports, 2016, 6, pp.37274 - 37274. ⟨10.1038/srep37274⟩

Article dans une revue inserm-01398571v1
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Affitins for protein purification by affinity magnetic fishing

Claudia S. M. Fernandes , Raquel dos Santos , Stella Ottengy , Aline Canani Viecinski , Ghislaine Béhar et al.

Journal of Chromatography A, 2016, 1457, pp.50-8. ⟨10.1016/j.chroma.2016.06.020⟩

Article dans une revue inserm-01342446v1
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Affitins as robust tailored reagents for affinity chromatography purification of antibodies and non-immunoglobulin proteins

Ghislaine Béhar , Axelle Renodon-Cornière , Barbara Mouratou , Frédéric Pecorari

Journal of Chromatography A, 2016, 1441, pp.44-51. ⟨10.1016/j.chroma.2016.02.068⟩

Article dans une revue inserm-01292506v1
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Biotechnological Uses of Archaeal Proteins

Frédéric Pecorari , Vickery Arcus , Juergen Wiegel

Archaea, 2015, 2015, pp.809758. ⟨10.1155/2015/809758⟩

Article dans une revue inserm-02913698v1
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Draft Genome Sequence of Erythromycin-Resistant Streptococcus gallolyticus subsp. gallolyticus NTS 31106099 Isolated from a Patient with Infective Endocarditis and Colorectal Cancer

Stanimir Kambarev , Clément Caté , Stéphane Corvec , Frédéric Pecorari

Genome Announcements, 2015, 3 (2), pp.e00370-15. ⟨10.1128/genomeA.00370-15⟩

Article dans une revue inserm-02913669v1
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Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes

Sabino Pacheco , Emiliano Cantón , Fernando Zuñiga‑navarrete , Frédéric Pecorari , Alejandra Bravo et al.

AMB Express, 2015, 5 (1), pp.73. ⟨10.1186/s13568-015-0160-1⟩

Article dans une revue inserm-01819791v1
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Artificial Affinity Proteins as Ligands of Immunoglobulins

Barbara Mouratou , Ghislaine Béhar , Frédéric Pecorari

Biomolecules, 2015, 5 (1), pp.60 - 75. ⟨10.3390/biom5010060⟩

Article dans une revue inserm-01818739v1

Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR

Sabino Pacheco , Ghislaine Behar , Mike Maillasson , Barbara Mouratou , Frédéric Pecorari

Protein Engineering, Design and Selection, 2014, 27 (10), pp.431-438. ⟨10.1093/protein/gzu042⟩

Article dans une revue hal-04969077v1

Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability

Ghislaine Béhar , Sabino Pacheco , Mike Maillasson , Barbara Mouratou , Frédéric Pecorari

Journal of Biotechnology, 2014, 192, pp.123-129. ⟨10.1016/j.jbiotec.2014.10.006⟩

Article dans une revue hal-04968943v1
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Potent and Specific Inhibition of Glycosidases by Small Artificial Binding Proteins (Affitins)

Agustín Correa , Sabino Pacheco , Ariel E Mechaly , Gonzalo Obal , Ghislaine Béhar et al.

PLoS ONE, 2014, 9 (5), pp.e97438. ⟨10.1371/journal.pone.0097438⟩

Article dans une revue hal-04969159v1

Tolerance of the archaeal Sac7d scaffold protein to alternative library designs: characterization of anti-immunoglobulin G Affitins

Ghislaine Behar , Marco Bellinzoni , Mike Maillasson , Lauranne Paillard-Laurance , Pedro M. Alzari et al.

Protein Engineering, Design and Selection, 2013, 26 (4), pp.267-275. ⟨10.1093/protein/gzs106⟩

Article dans une revue pasteur-03137902v1

Engineering of a phosphorylatable tag for specific protein binding on zirconium phosphonate based microarrays

Mathieu Cinier , Marc Petit , Frederic Pecorari , Daniel R. Talham , Bruno Bujoli et al.

Journal of Biological Inorganic Chemistry, 2012, 17 (3), pp.399-407. ⟨10.1007/s00775-011-0863-y⟩

Article dans une revue istex hal-02142701v1
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Reagentless fluorescent biosensors from artificial families of antigen binding proteins

Frederico F Miranda , Elodie Brient-Litzler , Nora Zidane , Frédéric Pecorari , Hugues Bedouelle

Biosensors and Bioelectronics, 2011, 26 (10), pp.4184-4190. ⟨10.1016/j.bios.2011.04.030⟩

Article dans une revue hal-05317582v1
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Llama V(H)H antibody fragments against GFAP: better diffusion in fixed tissues than classical monoclonal antibodies.

Claire Perruchini , Frederic Pecorari , Jean-Pierre Bourgeois , Charles Duyckaerts , François Rougeon et al.

Acta Neuropathologica, 2009, 118 (5), pp.685-95. ⟨10.1007/s00401-009-0572-6⟩

Article dans une revue istex pasteur-00429632v1

Bisphosphonate Adaptors for Specific Protein Binding on Zirconium Phosphonate-based Microarrays.

Frédéric Pecorari

Bioconjug Chem., 2009, 20 (12), pp.2270-7

Article dans une revue hal-00459484v1
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Type II secretion system secretin PulD localizes in clusters in the Escherichia coli outer membrane.

Nienke Buddelmeijer , Martin Krehenbrink , Frédéric Pecorari , Anthony P Pugsley

Journal of Bacteriology, 2009, 191 (1), pp.161-168. ⟨10.1128/JB.01138-08⟩

Article dans une revue hal-00414292v1

Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD.

M. Krehenbrink , M. Chami , I. Guilvout , Pedro Maria Alzari , F. Pécorari et al.

Journal of Molecular Biology, 2008, 383 (5), pp.1058-68. ⟨10.1016/j.jmb.2008.09.016⟩

Article dans une revue istex hal-00414255v1

Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD.

B. Mouratou , F. Schaeffer , I. Guilvout , D. Tello-Manigne , Anthony Paul Pugsley et al.

Proceedings of the National Academy of Sciences of the United States of America, 2007, 104 (46), pp.17983-8

Article dans une revue hal-00412884v1

Structures of in Vitro Evolved Binding Sites on Neocarzinostatin Scaffold Reveal Unanticipated Evolutionary Pathways

Antoine Drevelle , Marc Graille , Bernadette Heyd , Isabelle Sorel , Nathalie Ulryck et al.

Journal of Molecular Biology, 2006, 358, pp.455 - 471. ⟨10.1016/j.jmb.2006.02.002⟩

Article dans une revue istex hal-03299317v1

Selection and Characterization of Her2 Binding-designed Ankyrin Repeat Proteins

Christian Zahnd , Frédéric Pecorari , Nadine Straumann , Emanuel Wyler , Andreas Plückthun

Journal of Biological Chemistry, 2006, 281 (46), pp.35167-35175

Article dans une revue hal-00412873v1

Development of modified siRNA lipid nanocapsules - Evaluation of cellular uptake and stealth properties

Pauline Resnier , Jérôme Bejaud , Stephanie David , Pierre Legras , Thierry Benvegnu et al.

9th annual wold meeting on Pharmaceutics, Biopharmaceuticsand Pharmaceutical Technology, 2014, Lisbonne, Portugal. 2014

Poster de conférence hal-03184087v1

Biodistribution, tumor targeting and melanoma anticancer properties of innovative pegylated siRNA lipid nanocapsules

Pauline Resnier , Anthea Emina , Natacha Galopin , Jérôme Bejaud , Stephanie David et al.

Workshop SFNano "Tools and models for translation of Drug Delivery Systems", 2014, Porto, Portugal. 2014

Poster de conférence hal-03184099v1

Etude de l'impact sur l'internalisation cellulaire et le comportement sanguin de nanocapsules de siRNA modifiées en surface

Pauline Resnier , Jérôme Bejaud , Pierre Legras , Thierry Benvegnu , Frédéric Pecorari et al.

8èmes journées du Cancéropôle Grand Ouest, 2014, Les Sables d'Olonne, France. 2014

Poster de conférence hal-03137646v1

Development of smart siRNA Lipid NanoCapsules

Pauline Resnier , Jérôme Bejaud , C Gaillard , Pierre Legras , Nolwenn Lautram et al.

28th annual meeting of the GTRV, 2013, Orleans, France. 2013

Poster de conférence hal-03137655v1