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Harold Duruflé

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RESEARCH EXPERIENCE =================== 2021 **Research Scientist: Integrative genomic prediction of complex phenotypes using system biology approach** **-** Integrated Biology for the Development of Tree and Forest Diversity Laboratory, UMR INRAE/ONF 0588 2017 **PostDoc position: Develop the systems biology approach within the [SUNRISE project](https://www6.inrae.fr/sunrise/What-is-SUNRISE) ; 3 years** \- Laboratory of Plant-Microbe Interactions, UMR 2594/441 CNRS/INRA, Toulouse, France \- Team: Sunflower Genetics and Genomics (N. Langlade) 2014 **PhD: “Production and treatment of heterogeneous Omics data in order to study the cell wall plasticity in *A. thaliana* ecotypes living at contrasted altitudinal level”; 3 years** \- Plant Science Research Laboratory, UMR 5546 UPS/CNRS, Toulouse, France (Pr C. Dunand) \- Toulouse Mathematics Institute, France (Pr P. Besse) 2014 **Crop ontology curator. Bioversity International, Montpellier Office (France); 10 months** Team: Crop Ontology project of the Generation Challenge Programme (E. Arnaud) Internship: 2013 **Station Biologique de Roscoff, Marine Plants and Biomolecules, UMR 7139 UPMC/CNRS; 6 months** 2012 **Laboratory “Ecosystems, Biodiversity, Evolution”, UMR 6553 Rennes 1 University/CNRS; 3 months** 2011 **Laboratory of Plants Ecophysiological Responses to Environmental Stress, INRA Montpellier; 6 months** 2010 **Laboratory “Ecology Systematic Evolution”, Paris-Sud 11 University; 4 months** Team: Photosynthesis, environment and adaptation (P. Streb & G. Cornic) 2004-2011 **Joseph Fourier Alpine Station, Alpine Botanical garden of Lautaret; Internship 2 months / year** EDUCATION ========== 2013 **M.Sc. Degree: Integrative plant biology: Gene, Plant, Agro-system** 6 collaborating universities & Agrocampus Ouest (France) *Major: Physiology/Metabolism and Eco-Physiology.* 2011 **BSc Degree: Functional Plant Biology** Montpellier 2 University (France) TEACHING and MENTORING ====================== **Supervision:** PhD: **2021-2022** - Abdou Rahmane Wade. Improved genome-based phenotypic predictions with a systems biology approach. (Co-doctoral advisor: Leopoldo Sanchez Rodriguez & Vincent Segura) **2022-on going** - Alexandre Duplan. Improvement of pan-genomic knowledge and prediction of complex phenotypes by an integrative multi-omics approach. (Co-doctoral advisor: Leopoldo Sanchez Rodriguez & Stephane Maury) PostDoc: **2022** - Xabi Cazenave : Postdoc: 11 months Internship: **2023** - Alae-Eddine LEKCHIRI, Master degree, Rennes University **2023** - Maya Doureradjam. Master degree, Montpellier University (France): 6 months **2022** - Julien Desforges. Master degree, Orleans University (France): 4 months **2018** - Claire Espérou. 4th year in mathematics and modelling engineering school of INSA, Toulouse (France): 3 months **2017** - Sébastien Viudes. Master degree, Toulouse University (France): 2 months **2016** - Merwann Selmani. 4th year in mathematics and modelling engineering school of INSA, Toulouse (France): 3 months **2015** - Duchesse Lacour Mbadinga Mbadinga. BSc. Degree, Toulouse University (France): 6 months Others: **2019** - Caroline Laffray. Engineer: 1 year **2018** - Charlotte Penouilh-Suzette. Engineer: 6 months **Teaching:** **2015 - 2017**: Toulouse University, biostatistics and biophysics at BSc. Degree, 70 h per year. PROFESSIONAL SERVICES ===================== International meeting organization committees of: \- The [IX European Plant Science Retreat](http://epsr.net/), 20 - 23 June **2017**, Toulouse (France) \- The [Workshop on Multivariate data integration using the R package mixOmics](http://mixomics.org/2016/05/12-14-sept-toulouse/), 12 - 14 September **2016**, Toulouse (France) \- The [Workshop on Crop Ontology and Phenotyping Data Interoperability](https://sites.google.com/a/cgxchange.org/cropontologycommunity/), 31 March - 4 April **2014**, Montpellier (France‎)
RESEARCH EXPERIENCE =================== 2021 **Research Scientist: Integrative genomic prediction of complex phenotypes using system biology approach** **-** Integrated Biology for the Development of Tree and Forest Diversity Laboratory, UMR INRAE/ONF 0588 2017 **PostDoc position: Develop the systems biology approach within the [SUNRISE project](https://www6.inrae.fr/sunrise/What-is-SUNRISE) ; 3 years** \- Laboratory of Plant-Microbe Interactions, UMR 2594/441 CNRS/INRA, Toulouse, France \- Team: Sunflower Genetics and Genomics (N. Langlade) 2014 **PhD: “Production and treatment of heterogeneous Omics data in order to study the cell wall plasticity in *A. thaliana* ecotypes living at contrasted altitudinal level”; 3 years** \- Plant Science Research Laboratory, UMR 5546 UPS/CNRS, Toulouse, France (Pr C. Dunand) \- Toulouse Mathematics Institute, France (Pr P. Besse) 2014 **Crop ontology curator. Bioversity International, Montpellier Office (France); 10 months** Team: Crop Ontology project of the Generation Challenge Programme (E. Arnaud) Internship: 2013 **Station Biologique de Roscoff, Marine Plants and Biomolecules, UMR 7139 UPMC/CNRS; 6 months** 2012 **Laboratory “Ecosystems, Biodiversity, Evolution”, UMR 6553 Rennes 1 University/CNRS; 3 months** 2011 **Laboratory of Plants Ecophysiological Responses to Environmental Stress, INRA Montpellier; 6 months** 2010 **Laboratory “Ecology Systematic Evolution”, Paris-Sud 11 University; 4 months** Team: Photosynthesis, environment and adaptation (P. Streb & G. Cornic) 2004-2011 **Joseph Fourier Alpine Station, Alpine Botanical garden of Lautaret; Internship 2 months / year** EDUCATION ========== 2013 **M.Sc. Degree: Integrative plant biology: Gene, Plant, Agro-system** 6 collaborating universities & Agrocampus Ouest (France) *Major: Physiology/Metabolism and Eco-Physiology.* 2011 **BSc Degree: Functional Plant Biology** Montpellier 2 University (France) TEACHING and MENTORING ====================== **Supervision:** PhD: **2021-2022** - Abdou Rahmane Wade. Improved genome-based phenotypic predictions with a systems biology approach. (Co-doctoral advisor: Leopoldo Sanchez Rodriguez & Vincent Segura) **2022-on going** - Alexandre Duplan. Improvement of pan-genomic knowledge and prediction of complex phenotypes by an integrative multi-omics approach. (Co-doctoral advisor: Leopoldo Sanchez Rodriguez & Stephane Maury) PostDoc: **2022** - Xabi Cazenave : Postdoc: 11 months Internship: **2023** - Alae-Eddine LEKCHIRI, Master degree, Rennes University **2023** - Maya Doureradjam. Master degree, Montpellier University (France): 6 months **2022** - Julien Desforges. Master degree, Orleans University (France): 4 months **2018** - Claire Espérou. 4th year in mathematics and modelling engineering school of INSA, Toulouse (France): 3 months **2017** - Sébastien Viudes. Master degree, Toulouse University (France): 2 months **2016** - Merwann Selmani. 4th year in mathematics and modelling engineering school of INSA, Toulouse (France): 3 months **2015** - Duchesse Lacour Mbadinga Mbadinga. BSc. Degree, Toulouse University (France): 6 months Others: **2019** - Caroline Laffray. Engineer: 1 year **2018** - Charlotte Penouilh-Suzette. Engineer: 6 months **Teaching:** **2015 - 2017**: Toulouse University, biostatistics and biophysics at BSc. Degree, 70 h per year. PROFESSIONAL SERVICES ===================== International meeting organization committees of: \- The [IX European Plant Science Retreat](http://epsr.net/), 20 - 23 June **2017**, Toulouse (France) \- The [Workshop on Multivariate data integration using the R package mixOmics](http://mixomics.org/2016/05/12-14-sept-toulouse/), 12 - 14 September **2016**, Toulouse (France) \- The [Workshop on Crop Ontology and Phenotyping Data Interoperability](https://sites.google.com/a/cgxchange.org/cropontologycommunity/), 31 March - 4 April **2014**, Montpellier (France‎)

Publications

Multi-omics Data Integration in the Context of Plant Abiotic Stress Signaling

Harold Duruflé , Sébastien Déjean
Plant Abiotic Stress Signaling, 2642, Springer US, pp.295-318, 2023, Methods in Molecular Biology, ⟨10.1007/978-1-0716-3044-0_16⟩
Book sections hal-04040408v1
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Genetic control of abiotic stress-related specialized metabolites in sunflower

Marco Moroldo , Nicolas Blanchet , Harold Duruflé , Stéphane Bernillon , Thierry Berton
BMC Genomics, 2024, 25 (1), pp.199. ⟨10.1186/s12864-024-10104-9⟩
Journal articles hal-04468891v1
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Comprehensive Organ-Specific Profiling of Douglas Fir (Pseudotsuga menziesii) Proteome

Caroline Teyssier , Odile Rogier , Stéphane Claverol , Florian Gautier , Marie-Anne Lelu-Walter
Biomolecules, 2023, 13 (9), pp.1400. ⟨10.3390/biom13091400⟩
Journal articles hal-04209576v1
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Sunflower Hybrids and Inbred Lines Adopt Different Physiological Strategies and Proteome Responses to Cope with Water Deficit

Harold Duruflé , Thierry Balliau , Nicolas Blanchet , Adeline Chaubet , Alexandra Duhnen
Biomolecules, 2023, 13 (7), pp.1110. ⟨10.3390/biom13071110⟩
Journal articles hal-04161450v1
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eQTLs are key players in the integration of genomic and transcriptomic data for phenotype prediction

Abdou Rahmane Wade , Harold Duruflé , Leopoldo Sanchez , Vincent Segura
BMC Genomics, 2022, 23 (1), pp.476. ⟨10.1186/s12864-022-08690-7⟩
Journal articles hal-04031548v1
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Gene regulatory network inference methodology for genomic and transcriptomic data acquired in genetically related heterozygote individuals

Lise Pomiès , Celine Brouard , Harold Duruflé , Élise Maigné , Clément Carré
Bioinformatics, 2022, 38 (17), pp.4127-4134. ⟨10.1093/bioinformatics/btac445⟩
Journal articles hal-03776333v1
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A powerful framework for an integrative study with heterogeneous omics data: from univariate statistics to multi-block analysis

Harold Duruflé , Merwann Selmani , Philippe Ranocha , Elisabeth Jamet , Christophe Dunand
Briefings in Bioinformatics, 2021, 22 (3), pp.bbaa166. ⟨10.1093/bib/bbaa166⟩
Journal articles hal-02921927v3
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Proteomic data from leaves of twenty-four sunflower genotypes under water deficit

Thierry Balliau , Harold Duruflé , Nicolas Blanchet , Melisande Blein-Nicolas , Nicolas Langlade
OCL Oilseeds and fats crops and lipids, 2021, 28, ⟨10.1051/ocl/2020074⟩
Journal articles hal-03139476v1
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Leaf metabolomic data of eight sunflower lines and their sixteen hybrids under water deficit

Thierry Berton , Stéphane Bernillon , Olivier Fernandez , Harold Duruflé , Amélie Flandin
OCL Oilseeds and fats crops and lipids, 2021, 28, 42 - 6 p. ⟨10.1051/ocl/2021029⟩
Journal articles hal-03321074v1
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Données transcriptomiques de feuilles de huit lignées de tournesol et de leurs 16 hybrides soumis à un stress hydrique

Louise Gody , Harold Duruflé , Nicolas Blanchet , Clément Carré , Ludovic Legrand
OCL Oilseeds and fats crops and lipids, 2020, 27, pp.48. ⟨10.1051/ocl/2020044⟩
Journal articles hal-02974900v1

RNA expression dataset of 384 sunflower hybrids in field condition

Charlotte Penouilh-Suzette , Lise Pomies , Harold Duruflé , Nicolas Blanchet , Fanny Bonnafous
OCL Oilseeds and fats crops and lipids, 2020, 27, pp.36. ⟨10.1051/ocl/2020027⟩
Journal articles hal-02912579v1
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An integrative Study Showing the Adaptation to Sub-Optimal Growth Conditions of Natural Populations of Arabidopsis thaliana: A Focus on Cell Wall Changes

Harold Duruflé , Philippe Ranocha , Thierry Balliau , Michel Zivy , Cécile Albenne
Cells, 2020, 9 (10), pp.2249. ⟨10.3390/cells9102249⟩
Journal articles hal-02965604v1
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Phenotypic Trait Variation as a Response to Altitude-Related Constraints in Arabidopsis Populations

Harold Duruflé , Philippe Ranocha , Duchesse Lacour Mbadinga Mbadinga , Sébastien Dejean , Maxime Bonhomme
Frontiers in Plant Science, 2019, 10, pp.430. ⟨10.3389/fpls.2019.00430⟩
Journal articles hal-02115698v1
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Transcriptomic and cell wall proteomic datasets of rosettes and floral stems from five Arabidopsis thaliana ecotypes grown at optimal or sub-optimal temperature

Harold Duruflé , Philippe Ranocha , Thierry Balliau , Christophe Dunand , Elisabeth Jamet
Data in Brief, 2019, 27, pp.104581. ⟨10.1016/j.dib.2019.104581⟩
Journal articles hal-02938744v1
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Phenotyping and cell wall polysaccharide composition dataset of five arabidopsis ecotypes grown at optimal or sub-optimal temperatures

Harold Duruflé , Cécile Albenne , Elisabeth Jamet , Christophe Dunand
Data in Brief, 2019, 25, pp.104318. ⟨10.1016/j.dib.2019.104318⟩
Journal articles hal-03121670v1
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Metabolomic characterization of sunflower leaf allows discriminating genotype groups or stress levels with a minimal set of metabolic markers

Olivier Fernandez , Maria Urrutia , Thierry Berton , Stéphane Bernillon , Catherine Deborde
Metabolomics, 2019, 15 (4), pp.56. ⟨10.1007/s11306-019-1515-4⟩
Journal articles hal-02487236v1
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Data describing the eco-physiological responses of twenty-four sunflower genotypes to water deficit

Nicolas Blanchet , Pierre Casadebaig , Philippe Debaeke , Harold Duruflé , Louise Gody
Data in Brief, 2018, 21, pp.1296-1301. ⟨10.1016/j.dib.2018.10.045⟩
Journal articles hal-02625452v1

Cell wall modifications of two Arabidopsis thaliana ecotypes, Col and Sha, in response to sub-optimal growth conditions

Harold Duruflé , Vincent Hervé , Philippe Ranocha , Thierry Balliau , Michel Zivy
Plant Science, 2017, 263, pp.183-193. ⟨10.1016/j.plantsci.2017.07.015⟩
Journal articles hal-03121655v1

Cell wall proteome analysis of Arabidopsis thaliana mature stems

Harold Duruflé , Hélène San Clemente , Thierry Balliau , Michel Zivy , Christophe Dunand
Proteomics, 2017, 17 (8), pp.1-5. ⟨10.1002/pmic.201600449⟩
Journal articles hal-01606770v1
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Proline hydroxylation in cell wall proteins: is it yet possible to define rules?

Harold Duruflé , Vincent Hervé , Thierry Balliau , Michel Zivy , Christophe Dunand
Frontiers in Plant Science, 2017, 8, ⟨10.3389/fpls.2017.01802⟩
Journal articles hal-02620851v1
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Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata

Andrés A Ritter , Léa Cabioch , Loraine A Brillet-Guéguen , Erwan A Corre , Audrey A Cosse
PLoS ONE, 2017, 12 (3), pp.e0173315. ⟨10.1371/journal.pone.0173315.s011⟩
Journal articles hal-01587420v1

An enlarged cell wall proteome of Arabidopsis thaliana rosettes.

Vincent Hervé , Harold Duruflé , Hélène San Clemente , Cécile Albenne , Thierry Balliau
Proteomics, 2016, 16 (24), pp.3183-3187. ⟨10.1002/pmic.201600290⟩
Journal articles pasteur-01421109v1
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Une approche de biologie des systèmes pour mieux comprendre le déterminisme génétique de la production et de la qualité du bois du peuplier

Harold Duruflé , Annabelle Déjardin , Véronique Jorge , Marie Pégard , Gilles Pilate
Journées Xylogenèse, Groupe de Travail Xylogenèse du GDR Sciences du Bois, Jun 2024, Orleans France, France
Conference papers hal-04628657v1
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Multi-omics systems biology approach to reveal heterosis and drought response genomic processes in sunflower

Harold Duruflé
Journées des OMIQUES VEGETALES, Les plateformes omiques végétales du plateau de Saclay, Jun 2024, Saclay, France, France
Conference papers hal-04628618v1

Integration of omics data: example of analysis in a plant biology context

Harold Duruflé
Journées du Groupe Vigne de BAP et ses amis, Nov 2023, Colmar, France
Conference papers hal-04300759v1

Multivariate analyses of heterogeneous omics data highlights the emergence of novel molecular and physiological strategies of drought response in sunflower.

Harold Duruflé , Thierry Balliau , Nicolas Blanchet , Melisande Blein-Nicolas , Brigitte Mangin
Omics Days, Feb 2020, Montpellier, France
Conference papers hal-03509300v1

Contribution of an integrative study to the understanding of plant adaptation to their environment: A focus on plant cell walls.

Harold Duruflé , Sébastien Dejean
Cell Wall Days, Oct 2020, Toulouse, France
Conference papers hal-03509280v1
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Introduction to Ontology Semantics & tools for data annotation

Harold Duruflé
2d Rencontres du réseau SPE (INRA), Mar 2019, Toulouse, France
Conference papers hal-03511777v1

Sunflower hybrids and inbred lines adopt different molecular and physiological strategies to respond to water deficit

Harold Duruflé , Thierry Balliau , Nicolas Blanchet , Melisande Blein-Nicolas , Adeline Chaubet
Green Proteome, Jun 2019, Paris, France
Conference papers hal-03509317v1

Statistical learning on heterogeneous omics data highlights the molecular basis of heterosis of drought response in sunflower

Harold Duruflé , Thierry Balliau , Nicolas Blanchet , Melisande Blein-Nicolas , A Chaubet
Plant and Animal Genome XXVII, Jan 2019, San Diego, United States
Conference papers hal-02391988v1

Proteome responses to water deficit in sunflower: genetic variability, heterosis and links with phenotypic traits.

Harold Duruflé , Thierry Balliau , Melisande Blein-Nicolas , Nicolas Blanchet , Adeline Chaubet
INPPO World Congress, Sep 2018, Padoue, Italy
Conference papers hal-03509356v1

Arabidopsis thaliana as a model plant to study acclimation to sub-optimal growth conditions: An integrative study combining omics approaches reveals the importance of the cell wall.

Harold Duruflé , Vincent Hervé , Thierry Balliau , Michel Zivy , Sébastien Dejean
INPPO World Congress, Sep 2018, Padoue, Italy
Conference papers hal-03509374v1

Cell wall adaptation to sub-optimal growth conditions of natural population of Arabidopsis.

Harold Duruflé , Philippe Ranocha , Sébastien Dejean , Thierry Balliau , Michel Zivy
11es Journées du Réseau Français des Parois, Jun 2017, Orléans, France
Conference papers hal-03509390v1

Analysis and integration of heterogeneous data from response to low temperature condition of two Arabidopsis thaliana ecotypes

Harold Duruflé , Vincent Hervé , Philippe Ranocha , Cécile Albenne , Sébastien Dejean
COST FA1306, Workgroup 2, Feb 2016, Versailles, France
Conference papers hal-03509455v1

Harmonizing trait classes in the crop ontology & aligning with plant and trait ontologies

Harold Duruflé , Marie-Angelique Laporte , Matteis Lucas , Elizabeth Arnaud
Workshop on Crop Ontology and Phenotyping Data Interoperability, Mar 2014, Montpellier, France
Conference papers hal-03509472v1
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What epigenetics can bring to (plant) physiologists and ecologists in a climate change context

Alexandre Duplan , Régis Fichot , Mamadou Dia Sow , Alain Delaunay , Isabelle Le Jan
Biotechnocentre, Oct 2023, Nouan-le-Fuzelier, France.
Conference poster hal-04283904v1
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Trees short-time and inter-annual epigenetic somatic memories in response to drought-rewatering cycles

Alexandre Duplan , Régis Fichot , Mamadou Dia Sow , Alain Delaunay , Isabelle Lejan
14th International Conference of the French Society of Plant Biology, Jul 2023, Marseille, France.
Conference poster hal-04157492v1
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Etude des variations géographiques, génétiques et temporelles de la phénologie de floraison chez le peuplier noir

Xabi Cazenave , Véronique Jorge , Marc Villar , Harold Duruflé
Premières journées doctorales de la forêt, Apr 2023, Blois, France.
Conference poster hal-04071607v1
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eQTLs are key players in the integration of genomic and transcriptomic data for phenotype prediction

Abdou Rahmane Wade , Harold Duruflé , Leopoldo Sanchez , Vincent Segura
B4EST International Conference: Managing Forest Genetic Resources (FGR) for an Uncertain Future, Jun 2022, Lisboa, Portugal. 2022
Conference poster hal-04540839v1
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Exploration du génome du peuplier noir : identification de variations structurales

Romane Guilbaud , Véronique Jorge , Odile Rogier , Leopoldo Sanchez Rodriguez , Patricia Faivre-Rampant
1. colloque INRAE Genomics, May 2022, Orléans, France.
Conference poster hal-03937688v1
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Short reads et trios, un duo gagnant pour l'identification de variations structurales chez le peuplier noir

Romane Guilbaud , Odile Rogier , Véronique Jorge , Harold Duruflé , Leopoldo Sanchez Rodriguez
Mini congrès, Jun 2022, Evry, France
Conference poster hal-03937654v1
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An integrative systems biology approach to study the genetic variability and the molecular involved in sunflower heterosis responses to drought stress

Harold Duruflé
GisBV symposium inter-PIA, Oct 2019, Paris, France
Conference poster hal-03511754v1
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A systems biology approach to study the genetic variability of proteome responses to water deficit in sunflower

Harold Duruflé , Thierry Balliau , Nicolas Blanchet , Mélisande Blein-Nicolas , Adeline Chaubet
First International Plant Systems Biology meeting, Sep 2018, Roscoff, France.
Conference poster hal-03511745v1
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Phenotypic trait variation in genetically distincts Arabidopsis thaliana populations from the Pyrenees Mountains highlight acclimation to environmental constraints.

Harold Duruflé , Philippe Ranocha , Duchesse Lacour Mbadinga Mbadinga , Sébastien Déjean , Maxime Bonhomme
9th European Plant Science Retreat, Mar 2017, Toulouse, France. , 1p
Conference poster hal-03511709v1
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Analysis and integration of heterogeneous data from response to low temperature condition of two Arabidopsis thaliana ecotypes

Harold Duruflé , Vincent Hervé , Philippe Ranocha , Cécile Albenne , Sébastien Déjean
Day of the Doctoral School SEVAB, Mar 2016, Toulouse, France
Conference poster hal-03511731v1
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Production and treatment of heterogeneous omics data in order to study the cell wall plasticity in various Pyrenean altitudinal A. thaliana ecotypes

Harold Duruflé , Vincent Hervé , Philippe Ranocha , Cécile Albenne , Josiane Chourré
Day of the Doctoral School SEVAB, Mar 2015, Toulouse, France
Conference poster hal-03511721v1