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Isabelle Fournier

Professor Senior Member Institut Universitaire de France Vice President for Doctoral Research at University of Lille
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Presentation

Prof. Fournier is Distinguished Professor at University of Lille, and she holds a position as co-director of the PRISM Inserm U1192 Lab and as Vice President for Doctoral Research for the university. She is a bioanalytical chemist specialized in clinical mass spectrometry and proteomics. She started research working on fundamental of MALDI during her PhD at University Pierre & Marie Curie in Paris first and then, as a postdoctoral fellow within the group of Prof. Michael Karas in Frankfurt. In 2002, she established her own group at University of Lille where she started developing MALDI MS Imaging. She contributed to the field with several developments including strategies for imaging of FFPE tissues and proteins, and the development of tagged probes for specific imaging of mRNA and antigens. In 2009, she was hired as Full Professor and awarded a Junior position at Institut Universitaire de France. Since 2012, she got interested by the development of Spatially Resolved Proteomics in combination to MALDI MSI for applications in oncology. Over the past 5 years, she also worked on the development of in vivo MS for guided surgery and intraoperative analysis. She confounded the Imabiotech Company which provides services in MALDI MSI and recently CELEOS that will commercialize the SpiderMass technology. She was recently distinguished by the international distinguish award from MSACL and was awarded a senior position at Institut Universitaire de France in 2019.

Research domains

Analytical chemistry Genomics [q-bio.GN] Imaging

Skills

mass spectrometry imaging proteomics spatial proteomics lipidomics health technology

Publications

Real-time glioblastoma tumor microenvironment assessment by SpiderMass for improved patient management.

Yanis Zirem , Léa Ledoux , Lucas Roussel , Claude Alain Maurage , Pierre Tirilly
Cell Reports Medicine, 2024, pp.101482. ⟨10.1016/j.xcrm.2024.101482⟩
Journal articles hal-04527077v1

The impact and future of artificial intelligence in medical genetics and molecular medicine: an ongoing revolution

Firat Ozcelik , Mehmet Sait Dundar , A. Baki Yildirim , Gary Henehan , Oscar Vicente
Functional and Integrative Genomics, 2024, Functional and Integrative Genomics, 24, pp.138. ⟨10.1007/s10142-024-01417-9⟩
Journal articles hal-04712663v1
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Neuronal A2A receptor exacerbates synapse loss and memory deficits in APP/PS1 mice

V. Gomez-Murcia , Agathe Launay , K. Carvalho , A. Burgard , C. Meriaux
Brain - A Journal of Neurology , 2024, Brain - A Journal of Neurology, 147 (8), pp.2691-2705. ⟨10.1093/brain/awae113⟩
Journal articles hal-04684882v1
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A co-culture system of macrophages with breast cancer tumoroids to study cell interactions and therapeutic responses

Antonella Raffo Romero , Lydia Ziane Chaouche , Sophie Salome , Nawale Hajjaji , Isabelle Fournier
Cell Reports Methods, 2024, Cell Reports Methods, 4 (6), pp.100792. ⟨10.1016/j.crmeth.2024.100792⟩
Journal articles hal-04718878v1
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Deciphering genetic and nongenetic factors underlying tumour dormancy: insights from multiomics analysis of two syngeneic MRD models of melanoma and leukemia

Marie-Oceane Laguillaumie , Sofia Titah , Aurélie Guillemette , Bernadette Neve , Frédéric Leprêtre
Biological Research, 2024, Biological Research, 57 (1), pp.59. ⟨10.1186/s40659-024-00540-y⟩
Journal articles hal-04718849v1

In Vivo and Real-Time Metabolic Profiling of Plant-Microbe Interactions in Leaves, Stems, and Roots of Bacterially Inoculated Chardonnay Plantlets using SpiderMass

Nina Ogrinc , Essaïd Ait Barka , Christophe Clément , Michel Salzet , Lisa Sanchez
Analytical Chemistry, 2024, Analytical Chemistry, 96 (34), pp.13821-13829. ⟨10.1021/acs.analchem.4c01470⟩
Journal articles hal-04718924v1
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Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome.

Alice Capuz , Sylvain Osien , Tristan Cardon , Melodie-Anne Karnoub , Soulaimane Aboulouard
Cell Death Dis, 2023, Cell Death Dis, 14, pp.526. ⟨10.1038/s41419-023-06037-y⟩
Journal articles hal-04400988v1
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Astrocytes express aberrant immunoglobulins as putative gatekeeper of astrocytes to neuronal progenitor conversion

Alice Capuz , Sylvain Osien , Melodie-Anne Karnoub , Soulaimane Aboulouard , Estelle Laurent
Cell Death and Disease, 2023, Cell Death and Disease, 14 (4), pp.237. ⟨10.1038/s41419-023-05737-9⟩
Journal articles hal-04278200v2
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Protocol to identify human subcellular alternative protein interactions using cross-linking mass spectrometry.

Diego-Fernando Garcia del Rio , Isabelle Fournier , Tristan Cardon , Michel Salzet
STAR Protocols, 2023, STAR Protocols, 4 (3), pp.102380. ⟨10.1016/j.xpro.2023.102380⟩
Journal articles hal-04400811v1

From precise surgery to precision surgery: The multiple dimensions of therapeutic precision for head and neck cancer

Antoine Galmiche , Zuzana Saidak , Emmanuel Babin , Esteban Brenet , Julien Davrou
Journal of Stomatology, Oral and Maxillofacial Surgery, 2023, ⟨10.1016/j.jormas.2022.11.018⟩
Journal articles hal-03881465v1

Development of a novel apigenin prodrug programmed for alkaline-phosphatase instructed self-inhibition to combat cancer.

D. Diamantis , A. D. Tsiailanis , C. Papaemmanouil , M. C. Nika , Z. Kanaki
Journal of Biomolecular Structure and Dynamics, 2023, Journal of Biomolecular Structure and Dynamics, pp.1-22. ⟨10.1080/07391102.2023.2247083⟩
Journal articles hal-04400762v1

Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome

Alice Capuz , Sylvain Osien , Tristan Cardon , Mélodie Anne Karnoub , Soulaimane Aboulouard
Cell Death and Disease, 2023, 14 (8), pp.526. ⟨10.1038/s41419-023-06037-y⟩
Journal articles hal-04476023v1
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Employing non-targeted interactomics approach and subcellular fractionation to increase our understanding of the ghost proteome

Diego-Fernando Garcia del Rio , Tristan Cardon , S. Eyckerman , Isabelle Fournier , Amelie Bonnefond
iScience, 2023, Iscience, 26, pp.105943. ⟨10.1016/j.isci.2023.105943⟩
Journal articles hal-04401042v1
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Comparing MS imaging of lipids by WALDI and MALDI: two technologies for evaluating a common ground truth in MS imaging.

Lea Ledoux , Yanis Zirem , Florence Renaud , Ludovic Duponchel , Michel Salzet
Analyst, 2023, Analyst, 148, pp.4982-4986. ⟨10.1039/d3an01096a⟩
Journal articles hal-04401014v2
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Prophylactic Radical Fimbriectomy with Delayed Oophorectomy in Women with a High Risk of Developing an Ovarian Carcinoma: Results of a Prospective National Pilot Study

Éric Leblanc , Fabrice Narducci , Gwénaël Ferron , Audrey Mailliez , Jean Yves Charvolin
Cancers, 2023, 15 (4), ⟨10.3390/cancers15041141⟩
Journal articles hal-04068556v1
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Establishment and characterization of canine mammary tumoroids for translational research.

Antonella Raffo Romero , Soulaimane Aboulouard , Emmanuel Bouchaert , Agata Rybicka , Dominique Tierny
BMC Biology, 2023, BMC Biology, 21, pp.23. ⟨10.1186/s12915-023-01516-2⟩
Journal articles hal-04401119v1
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Fallopian tube lesions as potential precursors of early ovarian cancer: a comprehensive proteomic analysis.

Maxence Wisztorski , Soulaimane Aboulouard , Lucas Roussel , Marie Duhamel , Philippe Saudemont
Cell Death and Disease, 2023, Cell Death and Disease, 14, pp.644. ⟨10.1038/s41419-023-06165-5⟩
Journal articles hal-04400929v1
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OpenProt 2.0 builds a path to the functional characterization of alternative proteins.

S. Leblanc , F. Yala , N. Provencher , J. F. Lucier , M. Levesque
Nucleic Acids Research, 2023, Nucleic Acids Research, ⟨10.1093/nar/gkad1050⟩
Journal articles hal-04423840v1

Correction: Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome

Alice Capuz , Sylvain Osien , Tristan Cardon , Mélodie Anne Karnoub , Soulaimane Aboulouard
Cell Death and Disease, 2023, 14 (10), pp.677. ⟨10.1038/s41419-023-06143-x⟩
Journal articles hal-04278155v1

Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome

Alice Capuz , Sylvain Osien , Tristan Cardon , Mélodie Anne Karnoub , Soulaimane Aboulouard
Cell Death and Disease, 2023, 14 (8), pp.526. ⟨10.1038/s41419-023-06037-y⟩
Journal articles hal-04528686v1
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Challenges in glioblastoma research: focus on the tumor microenvironment

Andreas Bikfalvi , Cristine Alves da Costa , Tony Avril , Jean-Vianney Barnier , Luc Bauchet
Trends in Cancer, 2023, 9 (1), pp.9-27. ⟨10.1016/j.trecan.2022.09.005⟩
Journal articles hal-03860005v1

Early Diagnosis: End-to-End CNN–LSTM Models for Mass Spectrometry Data Classification

Khawla Seddiki , Frédéric Precioso , Melissa Sanabria , Michel Salzet , Isabelle Fournier
Analytical Chemistry, 2023, 95 (36), pp.13431-13437. ⟨10.1021/acs.analchem.3c00613⟩
Journal articles hal-04459618v1
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Mass Spectrometry-Based Differentiation of Oral Tongue Squamous Cell Carcinoma and Nontumor Regions With the SpiderMass Technology

Nina Ogrinc , Christophe Attencourt , Emilien Colin , Ahmed Boudahi , Riad Tebbakha
Frontiers in Oral Health, 2022, 3, ⟨10.3389/froh.2022.827360⟩
Journal articles hal-03722795v1

The Antibody Dependant Neurite Outgrowth Modulation Response Involvement in Spinal Cord Injury

Alice Capuz , Mélodie-Anne Karnoub , Sylvain Osien , Mélanie Rose , Céline Mériaux
Frontiers in Immunology, 2022, 13, ⟨10.3389/fimmu.2022.882830⟩
Journal articles hal-04278167v1

Trop-2, Na+/K+ ATPase, CD9, PKCα, cofilin assemble a membrane signaling super-complex that drives colorectal cancer growth and invasion

Emanuela Guerra , Valeria Relli , Martina Ceci , Romina Tripaldi , Pasquale Simeone
Oncogene, 2022, 41 (12), pp.1795-1808. ⟨10.1038/s41388-022-02220-1⟩
Journal articles hal-04460581v1
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Direct In Vivo Analysis of CBD- and THC-Acid Cannabinoids and Classification of Cannabis Cultivars Using SpiderMass.

Nina Ogrinc , S. Schneider , A. Bourmaud , N. Gengler , Michel Salzet
Metabolites, 2022, Metabolites, 12, ⟨10.3390/metabo12060480⟩
Journal articles hal-04412905v1
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On-tissue spatially resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape

Stacy Alyse Malaker , Jusal Quanico , Antonella Raffo-Romero , Firas Kobeissy , Soulaimane Aboulouard
Cell Chemical Biology, 2022, 29 (1), pp.30-42.e4. ⟨10.1016/j.chembiol.2021.05.007⟩
Journal articles hal-04460596v1
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Spatial analysis of the glioblastoma proteome reveals specific molecular signatures and markers of survival.

Marie Duhamel , L. Drelich , Maxence Wisztorski , Soulaimane Aboulouard , Jean-Pascal Gimeno
Nature Communications, 2022, Nature Communications, 13, pp.6665. ⟨10.1038/s41467-022-34208-6⟩
Journal articles hal-04212126v1
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Path to clonal theranostics in luminal breast cancers

Nawale Hajjaji , Soulaimane Aboulouard , Tristan Cardon , Delphine Bertin , Yves-Marie Robin
Frontiers in Oncology, 2022, Frontiers in Oncology, 11, pp.802177. ⟨10.3389/fonc.2021.802177⟩
Journal articles hal-04022193v1
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Unveiling a ghost proteome in the glioblastoma non-coding rnas

Tristan Cardon , Isabelle Fournier , Michel Salzet
Frontiers in Cell and Developmental Biology, 2021, Frontiers in Cell and Developmental Biology, 9, pp.703583. ⟨10.3389/fcell.2021.703583⟩
Journal articles hal-04011459v1
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Apport des outils de chemobiologie à l’imagerie par spectrométrie de masse

David Touboul , Isabelle Fournier
L'Actualité Chimique, 2021
Journal articles hal-03840976v1
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Shedding Light on the Ghost Proteome

Tristan Cardon , Isabelle Fournier , Michel Salzet
Trends in Biochemical Sciences, 2021, 46 (3), pp.239-250. ⟨10.1016/j.tibs.2020.10.003⟩
Journal articles hal-04446903v1
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Robot-assisted spidermass for in vivo real-time topography mass spectrometry imaging

Nina Ogrinc , Alexandre Kruszewski , Paul Chaillou , Philippe Saudemont , Chann Lagadec
Analytical Chemistry, 2021, Analytical Chemistry, ⟨10.1021/acs.analchem.1c01692⟩
Journal articles hal-03990566v1
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Surfaceome proteomic of glioblastoma revealed potential targets for immunotherapy

Melanie Rose , Tristan Cardon , Soulaimane Aboulouard , Nawale Hajjaji , Firas Kobeissy
Frontiers in Immunology, 2021, Frontiers in Immunology, 12, pp.746168. ⟨10.3389/fimmu.2021.746168⟩
Journal articles hal-04009596v1
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Therapeutic anti-glioma effect of the combined action of pcsk inhibitor with the anti-tumoral factors secreted by poly (i:c)-stimulated macrophages

Melanie Rose , Marie Duhamel , Soulaimane Aboulouard , Firas Kobeissy , Dominique Tierny
Cancer Gene Therapy, 2021, Cancer Gene Therapy, 29, pp.22-36. ⟨10.1038/s41417-020-00286-1⟩
Journal articles hal-04009706v1
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Persistence of coxsackievirus b4 in pancreatic ? cells disturbs insulin maturation, pattern of cellular proteins, and dna methylation

Magloire Pandoua Nekoua , Antoine Bertin , Famara Sane , Jean-Pascal Gimeno , Isabelle Fournier
Microorganisms, 2021, Microorganisms, 9 (6), pp.1125. ⟨10.3390/microorganisms9061125⟩
Journal articles hal-03955619v1

Persistence of Coxsackievirus B4 in Pancreatic β Cells Disturbs Insulin Maturation, Pattern of Cellular Proteins, and DNA Methylation

Magloire Pandoua Nekoua , Antoine Bertin , Famara Sane , Jean-Pascal Gimeno , Isabelle Fournier
Microorganisms, 2021, 9 (6), pp.1125. ⟨10.3390/microorganisms9061125⟩
Journal articles inserm-04456755v1
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Protein kinase c activation drives a differentiation program in an oligodendroglial precursor model through the modulation of specific biological networks

Marina Damato , Tristan Cardon , Maxence Wisztorski , Isabelle Fournier , Damiana Pieragostino
International Journal of Molecular Sciences, 2021, International Journal of Molecular Sciences, 22 (10), pp.5245. ⟨10.3390/ijms22105245⟩
Journal articles hal-03967287v1
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The NANOTUMOR consortium - Towards the Tumor Cell Atlas

Florent Colin , Kristine Schauer , Ali Hamiche , Pierre Martineau , Jean-Paul Borg
Biology of the Cell, 2021, 113 (6), pp.272-280. ⟨10.1111/boc.202000135⟩
Journal articles hal-03169719v1

Neurotrauma investigation through spatial omics guided by mass spectrometry imaging: Target identification and clinical applications

Khalil Mallah , Kazem Zibara , Coline Kerbaj , Ali Eid , Nour Khoshman
Mass Spectrometry Reviews, 2021, 42 (1), pp.189-205. ⟨10.1002/mas.21719⟩
Journal articles hal-04443805v1
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In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer

Soulaimane Aboulouard , Maxence Wisztorski , Marie Duhamel , Philippe Saudemont , Tristan Cardon
Cell Reports Medicine, 2021, Cell Reports Medicine, 2 (6), pp.100318. ⟨10.1016/j.xcrm.2021.100318⟩
Journal articles hal-04009565v1
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Toward high spatially resolved proteomics using expansion microscopy

Lauranne Drelich , Soulaimane Aboulouard , Julien Franck , Michel Salzet , Isabelle Fournier
Analytical Chemistry, 2021, Analytical Chemistry, 93 (36), pp.12195-12203. ⟨10.1021/acs.analchem.0c05372⟩
Journal articles hal-04011475v1
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In utero exposure to metformin reduces the fertility of male offspring in adulthood

Mélanie C. Faure , Rita Khoueiry , Jusal Quanico , Hervé Acloque , Marie-Justine Guerquin
Frontiers in Endocrinology, 2021, 12, pp.1-18. ⟨10.3389/fendo.2021.750145⟩
Journal articles hal-03462578v1
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Direct water-assisted laser desorption/ionization mass spectrometry lipidomic analysis and classification of formalin-fixed paraffin-embedded sarcoma tissues without dewaxing

Nina Ogrinc , Pierre-Damien Caux , Yves-Marie Robin , Emmanuel Bouchaert , Benoit Fatou
Clinical chemistry, 2021, 67 (11), pp.1513-1523. ⟨10.1093/clinchem/hvab160⟩
Journal articles hal-04019390v1
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Carbonic Anhydrase XII Expression Is Modulated during Epithelial Mesenchymal Transition and Regulated through Protein Kinase C Signaling

Daniele Vergara , Sara Ravaioli , Eugenio Fonzi , Loredaria Adamo , Marina Damato
International Journal of Molecular Sciences, 2020, 21 (3), pp.715. ⟨10.3390/ijms21030715⟩
Journal articles inserm-02941786v1

Spinal Cord Injury: Animal Models, Imaging Tools and the Treatment Strategies

Dasa Cizkova , Adriana-Natalia Murgoci , Veronika Cubinkova , Filip Humenik , Zuzana Mojzisova
Neurochemical Research, 2020, 45 (1), pp.134-143. ⟨10.1007/s11064-019-02800-w⟩
Journal articles inserm-02941635v1
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Reference and Ghost Proteins Identification in Rat C6 Glioma Extracellular Vesicles

Adriana-Natalia Murgoci , Tristan Cardon , Soulaimane Aboulouard , Marie Duhamel , Isabelle Fournier
iScience, 2020, 23 (5), pp.101045. ⟨10.1016/j.isci.2020.101045⟩
Journal articles inserm-02941792v1
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Cumulative learning enables convolutional neural network representations for small mass spectrometry data classification

Khawla Seddiki , Philippe Saudemont , Frédéric Precioso , Nina Ogrinc , Maxence Wisztorski
Nature Communications, 2020, 11 (1), pp.5595. ⟨10.1038/s41467-020-19354-z⟩
Journal articles hal-03132326v1
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Mechanisms of innate events during skin reaction following intradermal injection of seasonal influenza vaccine

Jessica Gonnet , Lauranne Poncelet , Celine Meriaux , Elena Gonçalves , Lina Weiss
Journal of Proteomics, 2020, 216, pp.103670. ⟨10.1016/j.jprot.2020.103670⟩
Journal articles hal-03984601v2

Epigenetic Studies Revealed a Ghost Proteome in PC1/3 KD Macrophages under Antitumoral Resistance Induced by IL-10

Tristan Cardon , Bilgehan Ozcan , Soulaimane Aboulouard , Firas Kobeissy , Marie Duhamel
Journal articles hal-04445712v1
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Openprot 2021: deeper functional annotation of the coding potential of eukaryotic genomes

Marie A. Brunet , Jean-Francois Lucier , Maxime Levesque , Sebastien Leblanc , Jean-Francois Jacques
Nucleic Acids Research, 2020, Nucleic Acids Research, 49 (D1), pp.D380-D388. ⟨10.1093/nar/gkaa1036⟩
Journal articles hal-04028636v1
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Location of neonatal microglia drives small extracellular vesicles content and biological functions in vitro

Adriana-Natalia Murgoci , Marie Duhamel , Antonella Raffo-Romero , Khalil Mallah , Soulaimane Aboulouard
Journal of Extracellular Vesicles, 2020, 9 (1), pp.1727637. ⟨10.1080/20013078.2020.1727637⟩
Journal articles inserm-02941810v1
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Sars-cov-2 interactome with human ghost proteome: a neglected world encompassing a wealth of biological data

Tristan Cardon , Isabelle Fournier , Michel Salzet
Microorganisms, 2020, Microorganisms, 8 (12), pp.2036. ⟨10.3390/microorganisms8122036⟩
Journal articles hal-03966962v1

A Hidden Human Proteome Signature Characterizes the Epithelial Mesenchymal Transition Program

Daniele Vergara , Tiziano Verri , Marina Damato , Marco Trerotola , Pasquale Simeone
Current Pharmaceutical Design, 2020, 26 (3), pp.372-375. ⟨10.2174/1381612826666200129091610⟩
Journal articles inserm-02941785v1
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The Role of a Proprotein Convertase Inhibitor in Reactivation of Tumor-Associated Macrophages and Inhibition of Glioma Growth

Mélanie Rose , Marie Duhamel , Soulaimane Aboulouard , Firas Kobeissy , Emilie Le Rhun
Molecular Therapy - Oncolytics, 2020, 17, pp.31-46. ⟨10.1016/j.omto.2020.03.005⟩
Journal articles inserm-02940430v1
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Alternative proteins are functional regulators in cell reprogramming by PKA activation

Tristan Cardon , Julien Franck , Etienne Coyaud , Estelle Laurent , Marina Damato
Nucleic Acids Research, 2020, 48 (14), pp.7864-7882. ⟨10.1093/nar/gkaa277⟩
Journal articles inserm-02941812v1

A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program

Anna Maria Giudetti , Stefania de Domenico , Andrea Ragusa , Paola Lunetti , Antonio Gaballo
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 2019, 1864 (3), pp.344-357. ⟨10.1016/j.bbalip.2018.12.011⟩
Journal articles inserm-02941613v1
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Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation

T Arab , A Raffo-Romero , C van Camp , Q Lemaire , F Le Marrec-Croq
Journal of Extracellular Vesicles, 2019, 8 (1), pp.1603048. ⟨10.1080/20013078.2019.1603048⟩
Journal articles inserm-02941660v1

Optimized Sample Preparation Workflow for Improved Identification of Ghost Proteins

Tristan Cardon , Flore Hervé , Vivian Delcourt , Xavier Roucou , Michel Salzet
Analytical Chemistry, 2019, 92 (1), pp.1122-1129. ⟨10.1021/acs.analchem.9b04188⟩
Journal articles inserm-02941813v1

Water-assisted laser desorption/ionization mass spectrometry for minimally invasive in vivo and real-time surface analysis using SpiderMass

Nina Ogrinc , Philippe Saudemont , Julia Balog , Yves-Marie Robin , Jean-Pascal Gimeno
Nature Protocols, 2019, 14 (11), pp.3162-3182. ⟨10.1038/s41596-019-0217-8⟩
Journal articles inserm-02941578v1

Optimizing the substrate-mediated laser ablation of biological tissues: Quest for the best substrate material

T. Maulouet , B. Fatou , C. Focsa , M. Salzet , I. Fournier
Applied Surface Science, 2019, 473, pp.486-492. ⟨10.1016/j.apsusc.2018.12.173⟩
Journal articles hal-02406750v1

Canine Bone Marrow-derived Mesenchymal Stem Cells: Genomics, Proteomics and Functional Analyses of Paracrine Factors