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Louis-Stéphane IV Le Clercq

Post-Doctorant
100%
Libre accès
12
Documents
Affiliations actuelles
  • 473351
  • 58867
Identifiants chercheurs
Contact
Site web
  • https://sites.google.com/view/lsleclercq/

Présentation

My name is Louis-Stéphane Le Clercq and I am a South African national of French- Italian heritage. My interest in a career in the Biological Sciences was peeked when my high school biology teacher recommended I enter the Mittal Science Olympiad and placed in the top 50 nationally. I started my formal education with a Bachelors of Science (B.Sc.) from the University of Pretoria, after which I completed a B.Sc. Honours from which I graduated cum laude and received academic honorary colours. During this degree I also completed several short courses including training on chemical grades and laboratory safety, good laboratory practice (GLP) and good clinical practice (GCP). My main supervisor was Prof. Duncan Cromarty for a "mini-project" on measuring transcription factor activation and nuclear translocation in macrophage-like cultured cells. This was followed by a research based Masters of Science (M.Sc.) as further postgraduate education under the supervision of Dr. Sheila Bowyer and Prof. Sim Mayaphi, on a project that focused on the full genome PCR amplification and mass and parallel sequencing (NGS) of viral meta-populations in chronic infections. This project was funded through grants from the National Research Foundation (NRF) and Poliomyelitis Research Fund (PRF). During this degree I also completed additional courses in phylogenetics, bioinformatics, and laboratory automation. Following my masters, I completed a year long internship/stage by the DST-NRF at the National Zoological Gardens, SANBI. This period provided significant exposure to areas such as conservation genetics, zoology, and animal science, including a workshop on population genetics. I recently completed thesis research towards a Ph.D. degree in Genetics at the University of the Free State (UFS), with my main supervisor Dr. Desire Dalton (Teesside), in collaboration with Prof. Paul Grobler (UFS) and Prof. Antoinette Kotze (SANBI). My project focused on molecular clocks and avian migration models and biological clocks such as methylation and telomere attrition as age markers for wildlife. For the avian migration component, I used short range PCR and fragment analyses to study polymorphisms in circadian clock genes in two bird species in relation to geographic distribution and general population structure. For the second component, I designed a new assay using methylation patterns in five genes, quantified by bisulfite sequencing through mass-array fragmentation analyses, to model and empirically determine age in a carnivore. This study was funded by grants from the NRF and a core grant from SANBI. During my time at UFS I also participated in the "UFS Diamond League" program that focuses on helping graduates be career ready though providing added training on the writing of cover letters and CVs, tips for job searches and interviews, and assistance in creating and improving your LinkedIn profile. Participation in these activities were graded and I placed in the top 15 among institution wide participants. I am presently registered with the South African Council for Natural Scientific Professions (SACNASP) as a Candidate Scientist in Molecular Biology (Registration No. 145810).

Domaines de recherche

Biodiversité et Ecologie Biologie animale Génétique

Compétences

DNA extraction DNA quantitation PCR qPCR Sequencing NGS Bisulfite sequencing Python R Bioinformatics Statistics GIS

Publications

Image document

Methylation-based markers for the estimation of age in African cheetah, Acinonyx jubatus

Louis-Stéphane Le Clercq , Antoinette Kotzé , J. Paul Grobler , Desire Lee Dalton
Molecular Ecology Resources, 2024, 24, ⟨10.1111/1755-0998.13940⟩
Article dans une revue hal-04474850v1
Image document

Dataset generated in a systematic review and meta-analysis of biological clocks as age estimation markers in animal ecology

Louis-Stéphane Le Clercq , J Paul Grobler , Antoinette Kotzé , Desiré Lee Dalton
Data in Brief, 2023, 51 (109615), ⟨10.1016/j.dib.2023.109615⟩
Article dans une revue hal-04229681v1
Image document

ABCal: a Python package for author bias computation and scientometric plotting for reviews and meta-analyses

Louis-Stéphane Le Clercq
Scientometrics, 2023, 129, pp.581-600. ⟨10.1007/s11192-023-04880-6⟩
Article dans une revue hal-04308276v1
Image document

Time trees and clock genes: a systematic review and comparative analysis of contemporary avian migration genetics

Louis‐Stéphane Le Clercq , Gaia Bazzi , Jacopo G Cecere , Luca Gianfranceschi , Johannes Paul Grobler
Biological Reviews, 2023, 98 (4), pp.1051-1080. ⟨10.1111/brv.12943⟩
Article dans une revue hal-04116625v1
Image document

PAReTT: A Python Package for the Automated Retrieval and Management of Divergence Time Data from the TimeTree Resource for Downstream Analyses

Louis-Stéphane Le Clercq , Antoinette Kotzé , J. Paul Grobler , Desiré Lee Dalton
Journal of Molecular Evolution, 2023, 91 (4), pp.502-513. ⟨10.1007/s00239-023-10106-3⟩
Article dans une revue hal-04116629v1
Image document

Biological clocks as age estimation markers in animals: a systematic review and meta‐analysis

Louis‐Stéphane Le Clercq , Antoinette Kotzé , J. Paul Grobler , Desiré Lee Dalton
Biological Reviews, 2023, ⟨10.1111/brv.12992⟩
Article dans une revue hal-04142813v1