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Louis-Stéphane IV Le Clercq
Post-Doctorant
100%
Libre accès
12
Documents
Affiliations actuelles
- 473351
- 58867
Identifiants chercheurs
- louis-stephane-iv-le-clercq
- ResearcherId : AAO-9057-2021
- VIAF : 472162664553655002597
- ISNI : 0000000502704324
- Google Scholar : https://scholar.google.co.za/citations?user=aFHkVVgAAAAJ&hl=en
Site web
- https://sites.google.com/view/lsleclercq/
Présentation
My name is Louis-Stéphane Le Clercq and I am a South African national of French- Italian heritage. My interest in a career in the Biological Sciences was peeked when my high school biology teacher recommended I enter the Mittal Science Olympiad and placed in the top 50 nationally. I started my formal education with a Bachelors of Science (B.Sc.) from the University of Pretoria, after which I completed a B.Sc. Honours from which I graduated cum laude and received academic honorary colours. During this degree I also completed several short courses including training on chemical grades and laboratory safety, good laboratory practice (GLP) and good clinical practice (GCP). My main supervisor was Prof. Duncan Cromarty for a "mini-project" on measuring transcription factor activation and nuclear translocation in macrophage-like cultured cells.
This was followed by a research based Masters of Science (M.Sc.) as further postgraduate education under the supervision of Dr. Sheila Bowyer and Prof. Sim Mayaphi, on a project that focused on the full genome PCR amplification and mass and parallel sequencing (NGS) of viral meta-populations in chronic infections. This project was funded through grants from the National Research Foundation (NRF) and Poliomyelitis Research Fund (PRF). During this degree I also completed additional courses in phylogenetics, bioinformatics, and laboratory automation. Following my masters, I completed a year long internship/stage by the DST-NRF at the National Zoological Gardens, SANBI. This period provided significant exposure to areas such as conservation genetics, zoology, and animal science, including a workshop on population genetics.
I recently completed thesis research towards a Ph.D. degree in Genetics at the University of the Free State (UFS), with my main supervisor Dr. Desire Dalton (Teesside), in collaboration with Prof. Paul Grobler (UFS) and Prof. Antoinette Kotze (SANBI). My project focused on molecular clocks and avian migration models and biological clocks such as methylation and telomere attrition as age markers for wildlife. For the avian migration component, I used short range PCR and fragment analyses to study polymorphisms in circadian clock genes in two bird species in relation to geographic distribution and general population structure. For the second component, I designed a new assay using methylation patterns in five genes, quantified by bisulfite sequencing through mass-array fragmentation analyses, to model and empirically determine age in a carnivore. This study was funded by grants from the NRF and a core grant from SANBI. During my time at UFS I also participated in the "UFS Diamond League" program that focuses on helping graduates be career ready though providing added training on the writing of cover letters and CVs, tips for job searches and interviews, and assistance in creating and improving your LinkedIn profile. Participation in these activities were graded and I placed in the top 15 among institution wide participants.
I am presently registered with the South African Council for Natural Scientific Professions (SACNASP) as a Candidate Scientist in Molecular Biology (Registration No. 145810).
Domaines de recherche
Biodiversité et Ecologie
Biologie animale
Génétique
Compétences
DNA extraction
DNA quantitation
PCR
qPCR
Sequencing
NGS
Bisulfite sequencing
Python
R
Bioinformatics
Statistics
GIS
Publications
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Molecular age estimation based on promotor CpG methylation using Methylation Sensitive PCR9th Annual Research Symposium of the National Zoological Gardens of South Africa, Nov 2018, Pretoria, South Africa. ScienceOpen Posters, 2022, ⟨10.14293/S2199-1006.1.SOR-.PPROAEF.v1⟩
Poster de conférence
hal-03337417v1
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Full Genome Amplification and Quasispecies Reconstruction of the Hepatitis B Virus from Next Generation Sequencing DataFaculty Day 2014, Aug 2014, Pretoria, South Africa. ⟨10.13140/RG.2.2.24117.04322⟩
Poster de conférence
hal-04179539v1
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Intra-host quasispecies reconstructions resemble inter-host variability of transmitted chronic hepatitis B virus strains2023
Pré-publication, Document de travail
hal-04099060v1
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Molecular characterization of full genome hepatitis b virus sequences from an urban hospital cohort in Pretoria, South AfricaVirology. University of Pretoria, 2014. English. ⟨NNT : ⟩
Thèse
tel-03334742v1
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