NICOLAS ROUHIER
Présentation
I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins. After a PhD in plant physiology, molecular biology and biochemistry, I have been recruited in 2004 as an assistant professor at the Université of Nancy, obtained an habilitation (HDR) in 2007 before gettting a professor position in 2011 at the newly created Université of Lorraine. For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with Populus trichocarpa and Arabidopsis thaliana as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles. In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX. The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations. Collaborators: C. Didierjean (Univ. Lorraine), JM. Herrmann (Kaiserslautern), S. Lemaire (CNRS – Sorbonne Université), A. Meyer (Bonn), B. Morgan (Saarbrücken), P. Rey (CEA Cadarache) The general objective here is to understand the functioning of the Fe-S cluster assembly machineries in organelles and more precisely the molecular mechanisms controlling the second step, i.e. the trafficking of preformed Fe-S clusters from scaffold proteins (made in the first step) to final acceptors. After focusing for some time on the capacity of several plant GRXs to assemble [2Fe-2S] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. Particular emphasis is given to the maturation of the photosynthetic and respiratory electron transfer complexes. Collaborators: J. Balk (JIC, Norwich), MK. Johnson (Athens, USA), O. Keech (Umea), A. Meyer (Bonn), U. Mühlenhoff (Marburg), C. Remacle (Liège), F. Vignols (BPMP Montpellier) Glutathione transferases (GSTs) constitute large multigenic families of more than 50 genes in terrestrial plants. They have three major biochemical functions. Those having a serinyl residue in their catalytic site (Ser-GSTs) possess GSH-conjugation activity. This catalytic activity is often described as required for xenobiotic detoxification. Those having a cysteinyl residue in their catalytic site (Cys-GSTs) possess the ability to catalyze the reverse reaction, i.e., the removal of glutathione from a number of structurally different heterocyclic molecules. The third function is the binding and/or transport of molecules/specialized metabolites without catalytic transformation, so-called ligandin function. It can be performed both by Ser-GSTs and Cys-GSTs. Although they are present in all kingdoms, the physiological functions of most plant GSTs remain elusive. The current work is centered on the structure-function analysis of specific classes of glutathione transferases, focusing in particular on the isolation and identification of physiological substrates explaining their implication in biotic or abiotic stress responses. In particular, we are interested in GSTs regulated during poplar leaf infection by the rust fungus Melampsora larici-populina or regulated during iron starvation in A. thaliana. The strategies used are to perform pull-down and affinity chromatographies, inhibition kinetics and thermal denaturation combining fluorescent probes and model ligands or plant extracts that are eventually separated by liquid ichromatography. We will not only focus on the previously characterized Cys-GSTs (lambda GSTs and glutathionyl hydroquinone reductases) but also on less explored or newly identified GST families. The nature (ligandin vs catalytic transformation) and the strength of the interactions will be validated by measuring binding constants using glutathionylated and non-glutathionylated molecules using biophycal approaches (ITC, Switchsense, fluorimetry). In parallel, the structure of GST alone or in complex with their ligand/substrate will be tentatively solved. Overall, we expect to decipher new intermediary steps of xenobiotic detoxification or of metabolic pathways requiring glutathione-conjugating or transport activities. New research questions have emerged recently from the observation that tau GST are able to bind porphyrins and hemes b in particular. Collaborators: C. Didierjean (Univ. Lorraine), C. Dubos (BPMP, Montpellier), S. Lemaire (CNRS – Sorbonne Université) Grants -2019-2022 : Projet ANR-2018: MITOGLU: Dissecting the molecular interactions of mitochondrial glutaredoxin S15 in plants (coordinators N. Rouhier/A. Meyer) -2018-2021 : Projet Lorraine Université Excellence, PEPS Mirabelle + : FeSprot: Maturation and structure-function analyses of iron-sulfur proteins (coordinator N. Rouhier) – 2018-2021: Projet ANR 2017: MOBIFER: Dynamics of coumarin secretion by plant roots into the soil in the context of iron nutrition (coordinator C. Dubos) – 2014-2017: Projet ANR 2013: Fe-S traffic: The cellular trafficking of iron-sulfur (Fe-S) clusters in plants (coordinator N. Rouhier) – 2011-2013: Projet ANR 2010: FIRES: Functions of glutaredoxins in Iron and REdox sensing and Signaling in plants (coordinator N. Rouhier) – 2009-2011: Projet INRA/FORMAS: The role of organellar glutaredoxins in iron-sulfur cluster biogenesis in plants: an interactomic study for the identification of protein partners (G. Wingsle (Umea Plant Science center, sweden)/ coordinator N. Rouhier) – 2008-2010: Projet ANR Jeune Chercheur: The role of organellar CGFS containing glutaredoxins in iron-sulfur clusters assembly and biogenesis in plants (coordinator N. Rouhier) – 2006-2008: Projet ANR, Réseau de Génomique végétale-Genoplante 2010: GNP05010P: Repair mechanisms of oxidized proteins in chloroplasts of Arabidopsis thaliana (coordinator Pascal Rey)
Publications
Publications
The chloroplastic Fe-S cluster maturation factors from Chlamydomonas, NFU1 and HCF101, have partially overlapping functions both in dark and light conditions21st International Conference on the Cell and Molecular Biology of Chlamydomonas, Aug 2025, Münster (DE), Germany |
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The hexameric atypical thioredoxin from poplar, DCC1,possesses a redox holdase activityCongrès annuel de la SFBBM, SFBBM, Jun 2025, Paris, France |
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Molecular mechanisms of the assembly of iron-sulfur clusters by the SUF machinery in chloroplastsInternational congress of the French society of photosynthesis. “The Many Facets of Photosynthesis”, May 2025, Paris, France |
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CyReB, a biosensor dedicated to the dynamic monitoring of the intracellular cysteine poolEMBO Workshop Thiol-based redox switches: From chemistry to physiology and pathology, Sep 2025, Sant Feliu De Guixols, Spain |
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Role of NFU1 and HCF101 in the insertion of [4Fe-4S] clusters in plastidial proteins of the green microalga Chlamydomonas reinhardtiiCOST FeSImmChemNet scientific meeting, Apr 2025, Belgrade, Serbia |
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Biochemical characterization of thioredoxin-related protein Clot/TRP14 of Populus trichocarpaSBIO2024 – 3rd Plant and Human sulfur meeting, Sep 2024, Pont-à-Mousson, France |
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Insertion of [4Fe-4S] clusters in plastidial proteins of Chlamydomonas reinhardtiiClosing Symposium of the DFG Priority Program SPP 1927 “Iron-sulfur for life”, Sep 2024, Potsdam (Germany), Germany |
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The plant DCC1 are atypical thioredoxins with a holdase activityPlant and Human Sulfur Biology Meeting, Nicolas Rouhier; Jérémy Couturier, Sep 2024, Pont-à-Mousson, France |
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Characterization of the chloroplastic NFU1 and HCF101, two [4Fe-4S] cluster transfer proteins of the sulfur mobilization (SUF) machinery in Chlamydomonas reinhardtii.Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting., Jun 2024, Winston Salem, United States |
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The plant atypical thioredoxins DCC1 possess chaperone activity.13th International Conference for Plant Mitochondrial Biology 2024, May 2024, Saint Malo, France |
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Assembly and trafficking of iron sulfur clusters by NFU proteins in plastids is essential for photosynthesis.Enzymes & Photosynthesis- Key for a green and sustainable future. Japanese-European Symposium, May 2024, Osaka, Japan |
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Integration of the iron-sulfur cluster transfer protein NFU1 of the green microalga Chlamydomonas in chloroplast metabolismCOST Action CA21115 – FeSImmChemNet’s Annual Meeting, Apr 2024, Prague, Czech Republic |
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Dynamic monitoring of the intracellular cysteine pool with the genetically-encoded biosensor CyReBPOG2024 - 16th International Conference on Reactive Oxygen and Nitrogen Species in Plants, May 2024, Antibes Juan-les-Pins, France |
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Monitoring intracellular dynamics of cysteine with the genetically-encoded biosensor CyReBCompartmentalized redox biology, meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2023, Düsseldorf, Germany |
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Are Trx-like 3 and 4 new players in the thiol-based redox metabolism in the chloroplast ?Compartmentalized redox biology, Jan Riemer & Carsten Berndt, Sep 2023, Düsseldorf, Germany |
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Functional characterization of high-chlorophyll fluorescence 101 (HCF101), a chloroplastic Fe-S cluster transfer protein in the green microalga Chlamydomonas reinhardtii7th Annual Meeting on Plant Ecology and Evolution (AMPEE7) "Small is beautiful, also in botany", Nov 2023, Liege, Belgium |
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Monitoring intracellular dynamics of cysteine with the genetically-encoded biosensor CyReBGDR NADPH Oxydase-Stress Oxydant & Club Oxydase, Nov 2023, Orsay, France |
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Roles of glutaredoxin S15 and BOLA4 in the maturation of iron-sulfur proteins in mitochondria.Congrès du Groupe Français de Bioénergétique, Sep 2023, Bédoin, France |
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Characterization of NFU1 and HCF101, two [4Fe-4S] cluster transfer proteins of the sulfur mobilization (SUF) machinery in Chlamydomonas chloroplast20th International Conference on the Cell and Molecular Biology of Chlamydomonas, Jun 2023, Princeton, United States |
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The critical role of glutaredoxin S15 for the maturation of iron-sulfur proteins in mitochondria.12th International Conference for Plant Mitochondrial Biology 2022, May 2022, Malmö, Sweden |
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The critical role of glutaredoxin S15 for the maturation of iron-sulfur proteins in mitochondria.Workshop “Metalloenzymes – cofactor assembly, catalytic mechanism and redox partners”., Nov 2022, Bochum, Germany |
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NAD(P)H cofactor electroenzymatic regeneration for selective biocatalytic reductions in a hybrid flow reactor73rd Annual Meeting of the International Society of Electrochemistry, Sep 2022, On Line, Switzerland |
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Role of the chloroplastic iron-sulfur transfer protein NFU1 in the microalga Chlamydomonas reinhardtiiBrussels Institute for Advanced Studies - Photosynthesis and primary production, Christian Hermans, Feb 2022, e-conference, Belgium |
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Maturation of iron-sulfur proteins in chloroplasts: focus on the NFU transfer proteinsSPS “Symposium on redox regulation and carbon-nitrogen interactions in plants. A tribute to Pierre Gadal”, Jun 2022, Gif sur Yvette, France |
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3-mercaptopyruvate sulfurtransferases: new actors in sulfur trafficking and H2S mediated-redox signalling in plants?Redox Biology Congress 2022 Oxidative stress, redox biology and antioxidants from plants to humans, Aug 2022, Ghent, Belgium |
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Rhodanese domain-containing sulfurtransferase family: a yet mysterious component of sulfur trafficking in plants12th International Plant Sulfur Workshop, Jul 2022, London, Canada |
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Redox properties of the plant atypical thioredoxin DCC1.Redox Biology Congress, Aug 2022, Ghent, Belgium |
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The chloroplastic iron-sulfur transfer protein NFU1 is required for chlorophyll synthesis in the dark and fermentation in the green microalga Chlamydomonas reinhardtiiIron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Sep 2022, Sainte Maxime, France |
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Gene atlas of iron-containing proteins (ironOME) in Arabidopsis thalianaIron Nutrition and Interactions in Plants, Jul 2022, Reims, France |
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Deciphering the biochemical relationships between cysteine desulfurase and sulfurtransferase protein families in sulfur trafficking12th International Plant Sulfur Workshop, Jul 2022, London, Canada |
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Identification of client proteins of the chloroplastic iron-sulfur transfer protein NFU2 in Arabidopsis thalianaJournées du Protéome Vert, INRAE, Jun 2022, Paris, France |
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Integration of the iron-sulfur transfer protein NFU1 of the microalga C. reinhardtii in chloroplast metabolism in the light, in the dark and in anaerobiosisCongrès du Groupe Français de Bioénergétique, online seminars, Sep 2021, online, France |
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Integration of the iron-sulfur transfer protein NFU1 of the microalga C. reinhardtii in chloroplast metabolism in the light, in the dark and in anaerobiosisChlamy 2020+1. 19th International Conference on the Cell and Molecular Biology of Chlamydomonas., Aug 2021, Six-fours-les-plages, France |
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The role of chloroplastic NFU in the green alga Chlamydomonas reinhardtiiOnline seminar series on “Iron-sulfur protein biogenesis 2021”, Jun 2021, e-seminar, France |
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Towards the identification of novel metal- or thiol-based regulated proteins in plantsInternational Symposium of the DFG Priority Program 1710 Dynamics of Thiol-based Redox Switches in Cellular Physiology, Mar 2021, e-conference, Germany |
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The role of chloroplastic NFU in the green alga Chlamydomonas reinhardtiiOnline seminar series on “Iron-sulfur protein biogenesis 2021”, Jun 2021, online, France |
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Role of the chloroplastic NFU3 iron-sulfur transfer protein in the microalga Chlamydomonas reinhardtii.International Bioenergy and environment congress, Feb 2021, e-conference, France |
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The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in plant mitochondria.Congrès du Groupe Français de Bioénergétique, Sep 2021, online seminars, France |
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Maturation of Fe-S proteins in plant organelles.12th congress of the French Society of Plant Biology, Jul 2019, Strasbourg, France |
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Are the ubiquitous mitochondrial glutaredoxin-like proteins of 12 kDa (MGP12) true glutaredoxins?Thiol-based switches and redox regulation – from microbes to men, Sep 2019, San Feliu de Guixols, Spain |
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Sulfur trafficking and H2S-mediated redox signaling in plants: roles of Arabidopsis 3-MP sulfurtransferasesSPP1710 Conference Thiol-based switches and redox regulation – from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain |
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The maturation of Fe-S proteins in chloroplasts and mitochondriaJacques Monod conference “Retrograde signalling from endosymbiotic organelles”, Oct 2018, Roscoff, France |
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Elucidating the function of the chloroplastic glutaredoxin S16 of Arabidopsis thaliana.12th Congress of the International Plant Molecular Biology, Aug 2018, Montpellier, France |
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Deciphering the biochemical properties of bacterial rhodanese fusion proteins.Fourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany |
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Functional characterization of mitochondrial NFU proteins in plants8th International Conference on Iron-Sulfur Cluster Biogenesis and Regulation, May 2018, Madison, United States |
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Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition18. International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan |
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How are Fe-S cofactors and proteins assembled in plant cells? focus on NFUs, involved in the late steps of the maturation process in organellesDays of the French society of photosynthesis, Jun 2017, Paris, France |
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Arabidopsis thaliana GRXS16, a multifaceted glutaredoxin in chloroplasts.3rd Redox Biology Symposium, Redox regulation: Historical background and future developments, Mar 2017, Nancy, France |
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Who are the partners of mitochondrial Arabidopsis thaliana GRXS15 in trafficking of iron-sulfur clusters?Redox meeting and Conversation, Mar 2017, Vandoeuvre Lès Nancy, France |
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The chloroplastic glutaredoxin S16: a plant-specific, two-domain protein with multiple biochemical properties.EMBO Conference on Redox Biology, Jul 2017, Moscou, Russia |
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Deciphering the late-steps of FeS cluster assembly in plant organelles: focus on NFUs.Kick off meeting of the COST action CA15133 FeSBioNET “The biogenesis of iron-sulfur proteins: from cellular biology to molecular aspects”., Sep 2016, Patras, Greece |
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Deciphering the molecular mechanisms of sulfur trafficking in plants.hird meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Jul 2016, Düsseldorf, Germany |
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Structure-function analysis of cysteine-containing glutathione transferases (GSTs) from Populus trichocarpaThiol-based redox switches in life sciences, EMBO-ESF meeting, Sep 2015, san feliu de guixols, Spain |
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The highly expanded thioredoxin and glutaredoxin classes in plants: specificities in the regeneration systems of enzymes using a sulfenic acid chemistryTheoretical and Practical Course “Redox Chemistry and Biology of Thiols” and associated symposium, Mar 2015, Montevideo, Uruguay |
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Exploring the partners of late-acting Fe-S transfer proteins belonging to the mitochondrial ISC machinery in Arabidopsis thaliana.Labex Mitocross 2015: mitochondria at the crossroad, Sep 2015, Strasbourg, France |
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Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexesIUBMB Symposium FeS 2015 - Iron Sulfur Cluster Biogenesis and Regulation, Jun 2015, Bergamo, Italy |
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Plastidial Nfu2 and Nfu3 are crucial for a proper root and shoot development in Arabidopsis thaliana7. International Conference on Iron-Sulfur Cluster Biogenesis and Regulation, Jun 2015, bergame, Italy |
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The multiple facets of glutaredoxins: one fold, several partners and functions12. International Conference on Reactive Oxygen and Nitrogen Species in Plants: from model systems to field, Jun 2015, Verona, Italy |
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Structure-function analyses of plant glutathione transferases with catalytic cysteine residuesTheoretical and Practical Course “Redox Chemistry and Biology of Thiols” and associated symposium., Mar 2015, montevideo, Uruguay |
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Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexes.IJB Symposium «Molecular Sciences, Facing Up to Major Societal Challenges»., Oct 2014, Nancy, France |
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Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexes.Gordon research conference “Thiol-based redox regulation and signalling”., Jul 2014, girone, Spain |
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Plastidial glutaredoxins: glutathione-dependent enzymes involved in detoxification, redox signalling and iron-sulfur cluster biogenesis.9th International Workshop - Sulfur Metabolism in Plants., Apr 2014, freiburg, Germany |
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Biochemical, spectroscopic and structural insights into glutaredoxins and their multiple types of interactions with BolAs2nd Symposium of the DFG Priority Program SPP 1710 “Dynamics of Thiol-based Redox Switches in Cellular Physiology”, Sep 2014, Heidelberg, Germany |
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Monothiol glutaredoxin and BolA in plants: two proteins with multiple interaction modes.9th International Workshop on Sulfur Metabolism in Plants: Molecular Physiology and Ecophysiology of Sulfur, Apr 2014, Freiburg, Germany |
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Genomics, transcriptomics and effectoromics of the poplar leaf rust fungus <em>Melampsora larici-populina</em>IUFRO Joint Meeting on Genetics of Five-Needle Pines and Rusts in Mountain Landscapes, Jun 2014, Fort Collins, United States |
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Investigation of the role of glutaredoxins and NFU proteins for the plastidial iron-sulfur cluster assembly machinary5. International Meeting of the Institute of Metals in Biology of Grenoble (IMBG), Sep 2012, Autrans, France |
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Les apports de la génomique pour l’étude des familles multigéniques : exemple des thaumatin-like proteins (TLPs) chez le peuplierSéminaire de l’Ecole Doctorale RP2E. Biodiversité, Ingénierie écologique, Jan 2011, Vandoeuvre-lès-Nancy, France. 8 p |
Expression of the chloroplastic FDX1 in different subcellular compartments of the green microalga Chlamydomonas reinhardtiiFeSImmChemNet COST scientific meeting, Apr 2025, Belgrade, Serbia |
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A new resource for the community: inventory of Chlamydomonas reinhardtii iron-sulfur proteins21st International Conference on the Cell and Molecular Biology of Chlamydomonas, Aug 2025, Münster (DE), Germany |
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Limitations of the fluorescent protein sensor roGFP2 for monitoring dehydroascorbate levels and dehydroascorbate reductase redox stateEMBO Workshop Thiol-based redox switches: From chemistry to physiology and pathology, Sep 2025, Sant Feliu De Guixols, Spain |
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The hexameric atypical thioredoxin from poplar, DCC1, possesses a redox holdase activityThiol oxidation in biology: Biochemical mechanisms to physiological outcomes, Sep 2025, Sant Feliu De Guixols, Spain |
Identifying potential actors promoting protein persulfidation in plants13th International Sulfur Workshop, Sep 2025, Heidelberg, France |
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The plant DCC1 are atypical thioredoxins with a holdase activity. Biochemical characterization of thioredoxin-like 3, a conserved protein in the green lineage3rd international conference on plant and human sulfur biology, Sep 2024, Pont-à-Mousson, France |
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Expression of the chloroplastic ferredoxin 1 in mitochondria of the green microalga Chlamydomonas reinhardtii.Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting., Jun 2024, Winston Salem, United States |
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Dynamic monitoring of the intracellular cysteine pool with the genetically-encoded biosensor CyReBSBIO2024 – 3rd Plant and Human sulfur meeting, Sep 2024, Pont-à Mousson, France |
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Expression and purification of Chlamydomonas reinhardtii hydrogenase HydA and maturation factors, HydG and HydEF.Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Jun 2024, Winston Salem, United States |
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Biochemical characterization of thioredoxin-related protein Clot/TRP14 of Populus trichocarpaSBIO2024 – 3rd Plant and Human sulfur meeting, Sep 2024, Pont-à-Mousson, France |
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Maturation of iron-sulfur containing subunits of respiratory complex I in plants.13th International Conference for Plant Mitochondrial Biology 2024, May 2024, Saint malo, France |
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Role of the of high-chlorophyll fluorescence 101 (HCF101), a chloroplastic Fe-S cluster transfer protein in the green microalga Chlamydomonas reinhardtiiIron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Jun 2024, Winston Salem, United States |
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Functional characterization of high-chlorophyll fluorescence 101 (HCF101), a chloroplastic Fe-S cluster transfer protein in the green microalga Chlamydomonas reinhardtiiJournées Françaises de la Photosynthèse" SPhi 2024, Mar 2024, Paris, France |
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Towards the understanding of the molecular mechanisms of the assembly of iron-sulfur clusters by the SUF machinery in chloroplastsXI International Conference on Fe-S Proteins: Function, Biogenesis, and Regulation, Jun 2024, Winston-Salem, United States |
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Characterization of thioredoxin-like 3, a conserved protein in the green lineagePlant Oxygen Group, May 2024, Juan-les-Pins / Antibes, France |
Biochemical characterization of thioredoxin-related protein Clot/TRP14 of Populus trichocarpaPOG2024 - 16th International Conference on Reactive Oxygen and Nitrogen Species in Plants, May 2024, Antibes Juan-les-Pins, France |
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Toward the characterisation of an unexplored class of glutaredoxins in photosynthetic eucaryotesVillage A2F, Jun 2024, Vandoeuvre -les-Nancy, France |
Biochemical characterization of thioredoxin-related protein Clot/TRP14 of Populus trichocarpaCompartmentalized redox biology, meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2023, Düsseldorf, Germany |
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Maturation of iron-sulfur containing subunits of respiratory complex I in plants3rd INUPRAG symposium, Feb 2023, Umea, Sweden |
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Are Trx-like 3 and 4 new players in the thiol-based redox metabolism in the chloroplast?Meeting on compartmentalized redox biology, Sep 2023, Dusseldorf, Germany |
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Development of a genetically-encoded biosensor to monitor an H2S-related metaboliteSociety for Redox Biology and Medicine (SfRBM) 30th Annual Conference and Society for Free Radical Research International (SFRRI) 21st Biennial Congress, Nov 2023, Punta del Este, Uruguay, Uruguay |
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Sulfurtransferase-mediated formation of H2S and persulfidation in Arabidopsis thalianaBotanik-Tagung International Conference of the German Society for Plant Sciences, Aug 2022, Bonn, Germany |
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The complex redox regulation of the SAL1 phosphatase present in plant organellesThiol oxidation in biology: Biochemical mechanisms to physiological outcomes, Oct 2022, sant feliu de guixols, Spain |
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Maturation of iron-sulfur-containing subunits of respiratory complex I in plantsIron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Sep 2022, Sainte Maxime, France |
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Exploiting a bacterial cysteine desulfurase-sulfurtransferase fusion to develop a roGFP2-based biosensor for free cysteineThiol oxidation in biology: Biochemical mechanisms to physiological outcomes, Oct 2022, San feliu de Guixols, Spain |
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Exploiting a bacterial cysteine desulfurase-sulfurtransferase fusion to develop a roGFP2-based biosensor for free cysteineRedox Biology Congress 2022 Oxidative stress, redox biology and antioxidants from plants to humans, Aug 2022, Ghent, Belgium |
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Cysteine catabolism and the role of sulfurtransferases in persulfidation reactions in ArabidopsisThiol oxidation in biology: Biochemical mechanisms to physiological outcomes, Oct 2022, San feliu de Guixols, Spain |
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Iron-sulfur cluster formation and transfer using plant mitochondrial glutaredoxin-BOLA complexes.Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting., Sep 2022, Sainte-maxime, France |
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Investigation of cellular interactions and biochemical characterization of the chloroplastic NFU1 transfer protein from Chlamydomonas reinhardtii.Chlamy 2020+1. 19th International Conference on the Cell and Molecular Biology of Chlamydomonas., Aug 2021, Six-Fours-les-Plages, France |
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Deciphering the biochemical relationships between cysteine desulfurase and sulfurtransferase protein families in sulfur traffickingS-Bio 2021 Glucosinolate Joint meeting for plant and human sulfur biology and glucosinolates, Sep 2021, Seville, Spain |
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Unravelling the role of an atypical mitochondrial ferredoxin from Arabidopsis thaliana.Congrès du Groupe Français de Bioénergétique, Sep 2021, online seminar, France |
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Integration of the chloroplastic iron-sulfur transfer protein NFU1 from Chlamydomonas reinhardtii in chlorophyll metabolism in the darkSfphi2021 : Journées de la Société Française de Photosynthèse, Apr 2021, online, France |
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Identification of client iron-sulfur proteins of the chloroplastic NFU3 transfer protein from Chlamydomonas reinhardtiiInternational Bioenergy and environment congress, Feb 2021, online, France |
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Shedding light on a H2S generating pathway in ArabidopsisS-Bio 2021 Glucosinolate Joint meeting for plant and human sulfur biology and glucosinolates, Sep 2021, Seville, Spain |
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Developing redox biosensors for sulfur compoundsSPP1710 Conference Thiol-based switches and redox regulation – from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain |
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Biochemical and structural characterization of A. thaliana glutathione transferases from the tau classThiol-based switches and redox regulation – from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain |
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Does Arabidopsis sulfurtransferase 18 participate to sulfur trafficking?Thiol-based switches and redox regulation - from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain |
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The α-amylase 3 of Arabidopsis thaliana is protected by S-glutathionylation in vitro114° Congresso della Società Botanica Italiana onlus, VI INTERNATIONAL PLANT SCIENCE CONFERENCE (IPSC)., Sep 2019, padova, Italy |
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Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition12ème Congrès de la Société Française de Biologie Végétale SFBV, Jul 2019, Strasbourg, France |
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MIA40 of Arabidopsis thaliana is essential for the optimal in vitro oxidation of TIM and COX proteinsFourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany |
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Decrypting the chloroplastic [fe-s] cluster assembly machinery12th Congress of the International Plant Molecular Biology, Aug 2018, Montpellier, France. , 2018 |
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Decrypting the chloroplastic [Fe-S] cluster assembly machinery18. International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan |
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Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition18. International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan |
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Deciphering the biochemical properties of plastidial GRXS14 and mitochondrial GRXS15 isoforms of Arabidopsis thalianaFourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany |
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Sulfur trafficking and H2S mediated-redox signalling in plants: Biochemical characterization of Arabidopsis thaliana 3-MP sulfurtransferases12th Congress of the International Plant Molecular Biology (IPMB), Aug 2018, Montpellier, France |
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Seeking the role of the plastidial NFU1 transfer protein in Arabidopsis thaliana.Iron-Sulfur Proteins-Biogenesis, Regulation and Function 39th Steenbock Symposium, May 2018, Madison, United States |
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Deciphering the biochemical properties of a bacterial ferredoxin-thioredoxin reductase-rhodanese fusion proteinFourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany |
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Functional characterization of Arabidopsis BolA proteins in iron-sulfur center transfer pathways and in iron homeostasis12th International Plant Molecular Biology Congress, Aug 2018, Montpellier, France |
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Biochemical characterization of glutathione transferases potentially involved in the metabolism of coumarins in Arabidopsis thaliana during iron deficiencyJacques Monod conference “Retrograde signalling from endosymbiotic organelles", Oct 2018, Roscoff, France |
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Biochemical characterization of glutathione transferases potentially involved in the metabolism of coumarins in Arabidopsis thaliana during iron deficiencyMeeting of the SPP1710/Study group redox biology of the GBM, Sep 2018, Berlin, Germany |
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Mitochondrial Fe-S cluster assembly in Arabidopsis thaliana: In vitro characterization of the properties and role of GRX, ISCA and NFU proteinsIron-Sulfur Proteins-Biogenesis, Regulation and Function 39th Steenbock Symposium, May 2018, Madison, United States |
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Mia40 is not just a “poor man’s thioredoxin”Thiol oxidation in toxicity and signalling., Sep 2017, Sant Feliu de Guixols, Spain |
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The cellular maturation of iron-sulfur proteins in plants1st FrenchBIC summer school, Methods for studying metals in biology, Sep 2017, Carry-le-Rouet, France |
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Towards the roles of Arabidopsis plastidial CGFS glutaredoxins: involvement of GRXS14 in the maintenance of chlorophyll content under specific physiological situations“Thiol oxidation in toxicity and signalling” Workshop, Sep 2017, Sant Feliu de Guixols, Spain |
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Comparison of the redox properties of plant and yeast MIA40/ERV1 couples“Thiol oxidation in toxicity and signalling” Workshop, Sep 2017, Sant Feliu de Guixols, Spain |
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Deciphering the biochemical properties of plant sulfurtransferases to dissect cellular sulfur trafficking mechanisms.“Thiol oxidation in toxicity and signalling” Workshop, Sep 2017, Sant Feliu de Guixols, Spain |
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Biochemical characterization of glutathione transferases potentially involved in the metabolism of coumarins in Arabidopsis thaliana during iron deficiencyThiol oxidation in toxicity and signalling, Sep 2017, Sant Feliu de Guixols, Spain |
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The cellular maturation of iron-sulfur proteins in plants1st FeSBioNet Training School, Jun 2017, Lisbonne, Portugal |
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Identification of mitochondrial ferredoxin partners from Arabidospis thaliana leaves18th International Symposium on Iron Nutrition and Interaction in Plants, Jun 2016, Madrid, Spain |
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Arabidopsis thaliana NFU proteins participate in the final steps of the maturation of mitochondrial iron-sulfur proteinsGordon Research Conferences on Thiol-Based Redox Regulation & Signaling, Jul 2016, Barga, Italy |
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Towards the function of the chloroplastic Arabidopsis thaliana glutaredoxin S163rd meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Jul 2016, Düsseldorf, Germany |
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Mitochondrial Arabidopsis thaliana NFU transfer proteins: cooperation with ISCA proteins to deliver [4Fe-4S] cluster to specific apo-targets18. International Symposium on Iron Nutrition and Interactions in Plants, May 2016, Madrid, Spain. 2016 |
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Structural characterization of BolA-metal complexes through Sinorhizobium meliloti YrbACongrès de l’Association Française de Cristallographie (AFC), Jul 2016, Marseille, France |
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Diverse structural forms involving BolA proteinsMosbacher Kolloquium - Metals in Biology: Cellular functions and diseases, Mar 2015, Mosbach, Germany |
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Structural investigation of poplar dehydroascorbate reductase by NMR spectroscopy2nd Redox meeting, 2015, Vandoeuvre lès Nancy, France |
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Exploring the functions and partners of mitochondrial ISCA transfer proteins from A. thalianaIUBMB Symposium FeS 2015 - Iron Sulfur Cluster Biogenesis and Regulation, Jun 2015, Bergamo, Italy |
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Exploring the functions and partners of mitochondrial ISCA transfer proteins from A. thalianaMosbacher Kolloquium - Metals in Biology: Cellular functions and diseases, Mar 2015, Mosbach, Germany |
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Deciphering the functions and partners of mitochondrial ferredoxins from Arabidopsis thalianaChemistry & Biology of Iron-Sulfur Clusters 6th International IMBG Meeting & Advanced Courses, Sep 2015, Villard de Lans, France |
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Arabidopsis thaliana plastidial Nfu2 and Nfu3 are required for the maturation of [4Fe-4S] clusters into photosystem7th International Conference on the Biogenesis of Iron Sulphur Proteins, May 2013, Columbia, United States. 2013 |
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Arabidopsis thaliana plastidial Nfu2 and Nfu3 are required for the maturation of [4Fe-4S] clusters into photosystem I7th International Conference on Iron-Sulfur Cluster Biogenesis and Regulation, May 2013, South Carolina, United States |
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NMR study of Arabidopsis thaliana BolA2 : structure, dynamics and interaction with glutaredoxinsEuromar Magnetic Resonance Conference, Jun 2013, Hersonissos, Greece |
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NMR study of Arabidopsis thaliana BolA2 : structure, dynamics and interaction with glutaredoxinsXXVth International Conference on Magnetic Resonance in Biological Systems, Aug 2012, Lyon, France |
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Study of Arabidopsis thaliana BolA2 : Structure, Dynamics and Interaction with GlutaredoxinsXXVth International Conference on Magnetic Resonance in Biological Systems, Aug 2012, Lyon, France |
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Clusters fer-soufre : rôle des glutarédoxines dans la machinerieGTBio, Apr 2012, Montpellier, France |
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Roles of glutaredoxins in iron-sulfur cluster biogenesis and intracellular iron sensing in plants5th International Meeting and Second Advanced Courses of the IMBG: Metals Homeostasis, Sep 2012, Autrans, France |
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Peroxide detoxification systems in poplarPlant Abiotic Stress tolerance II. Section Plant Response to Oxidative Stress, Feb 2012, Vienna, Austria |
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Structure/function analysis of plant BolAs, deciphering their interaction with glutaredoxinsBiogenesis of iron sulphur proteins and regulatory functions, Aug 2011, Cambridge, United Kingdom |
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Structural and functional characterization of a plastidial poplar glutaredoxin with an atypical WCSYS active site36ème Forum des Jeunes Chercheurs de la Société Française de Biochimie et de Biologie Moléculaire, Aug 2009, Nancy, France |
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Structural and functional characterization of a plastidial poplar glutaredoxin with an atypical WCSYS active siteGordon Research Conferences : Thio-Based Redox Regulation & Signalling, May 2008, Barga, Italy |
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Structural and functional characterization of a plastidial poplar glutaredoxin with an atypical WCSYS active siteGlutathione International Symposium: Glutathione and related thiols in microorganisms and plants, Aug 2008, Nancy, France |
Structural diversity and specificities within the ferredoxin disulfide/thioredoxin reductase familyJohn Wiley & Sons. Encyclopedia of Inorganic and bioinorganic chemistry, 2024, 9781119951438. ⟨10.1002/9781119951438.eibc2868⟩ |
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The mitochondrion: from genome to proteomeThe Chlamydomonas Sourcebook, Elsevier, pp.369-412, 2022, ⟨10.1016/B978-0-12-821430-5.00011-0⟩ |
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Thiol-based redox control in chloroplastsRedox Chemistry and Biology of Thiols, Elsevier, pp.507-532, 2022, ⟨10.1016/B978-0-323-90219-9.00023-6⟩ |
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News on the redox front—A follow-up of ABR volume 52: Oxidative stress and redox regulation in plantsAdvances in Botanical Research, 100, pp.355-378, 2021, ⟨10.1016/bs.abr.2021.01.010⟩ |
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FeS Cluster Assembly: Role of Monothiol Grxs and Nfu ProteinsEncyclopedia of Inorganic and Bioinorganic Chemistry, John Wiley & Sons, Ltd, 2017, ⟨10.1002/9781119951438.eibc2470⟩ |
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In Vitro Alkylation Methods for Assessing the Protein Redox StatePhotorespiration : Methods and Protocols, pp.51-64, 2017, ⟨10.1007/978-1-4939-7225-8_4⟩ |
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Redox HomeostasisUrsula Jakob; Dan Reichmann. Oxidative Stress and Redox Regulation, Springer Netherlands, pp.59-84, 2013, 978-94-007-5786-8. ⟨10.1007/978-94-007-5787-5_3⟩ |
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Redox Regulation in Plants: Glutathione and “Redoxin” Related FamiliesUrsula Jakob; Dan Reichmann. Oxidative Stress and Redox Regulation, Springer Netherlands, pp.213-231, 2013, 978-94-007-5786-8. ⟨10.1007/978-94-007-5787-5_8⟩ |
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Glutaredoxin: The Missing Link Between Thiol-Disulfide Oxidoreductases and Iron Sulfur EnzymesAdvances in Botanical Research: Oxidative stress and redox regulations in plants, 52, Elsevier, 2009, Advances in Botanical Research, 978-0-12-378622-7. ⟨10.1016/S0065-2296(10)52013-5⟩ |
Régulation rédox glutathion dépendante chez le peuplier : Isolement et caractérisation de la glutarédoxine2000, 22p |
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The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in mitochondria2022 |
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The mitochondrial ferredoxin-like is essential for the formation of complex I-containing respiratory supercomplexes in Arabidopsis thaliana2022 |
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Aspects fonctionnels et structuraux des systèmes thiorédoxine et glutarédoxine de peuplier et de leurs enzymes cibles, les peroxyrédoxines et les méthionine sulfoxyde réductases de type ABiologie végétale. Université Henri Poincaré - Nancy 1, 2003. Français. ⟨NNT : 2003NAN10104⟩ |