
NICOLAS ROUHIER
Présentation
I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins.
After a PhD in plant physiology, molecular biology and biochemistry, I have been recruited in 2004 as an assistant professor at the Université of Nancy, obtained an habilitation (HDR) in 2007 before gettting a professor position in 2011 at the newly created Université of Lorraine. For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with Populus trichocarpa and Arabidopsis thaliana as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles.
- Redox post-translational modifications of proteins
In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX.
The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations.
Collaborators: C. Didierjean (Univ. Lorraine), JM. Herrmann (Kaiserslautern), S. Lemaire (CNRS – Sorbonne Université), A. Meyer (Bonn), B. Morgan (Saarbrücken), P. Rey (CEA Cadarache)
- Molecular analysis of late steps of Fe-S cluster maturation in organelles
The general objective here is to understand the functioning of the Fe-S cluster assembly machineries in organelles and more precisely the molecular mechanisms controlling the second step, i.e. the trafficking of preformed Fe-S clusters from scaffold proteins (made in the first step) to final acceptors. After focusing for some time on the capacity of several plant GRXs to assemble [2Fe-2S] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. Particular emphasis is given to the maturation of the photosynthetic and respiratory electron transfer complexes.
Collaborators: J. Balk (JIC, Norwich), MK. Johnson (Athens, USA), O. Keech (Umea), A. Meyer (Bonn), U. Mühlenhoff (Marburg), C. Remacle (Liège), F. Vignols (BPMP Montpellier)
- Structure-Function analysis of glutathione transferases
Glutathione transferases (GSTs) constitute large multigenic families of more than 50 genes in terrestrial plants. They have three major biochemical functions. Those having a serinyl residue in their catalytic site (Ser-GSTs) possess GSH-conjugation activity. This catalytic activity is often described as required for xenobiotic detoxification. Those having a cysteinyl residue in their catalytic site (Cys-GSTs) possess the ability to catalyze the reverse reaction, i.e., the removal of glutathione from a number of structurally different heterocyclic molecules. The third function is the binding and/or transport of molecules/specialized metabolites without catalytic transformation, so-called ligandin function. It can be performed both by Ser-GSTs and Cys-GSTs. Although they are present in all kingdoms, the physiological functions of most plant GSTs remain elusive.
The current work is centered on the structure-function analysis of specific classes of glutathione transferases, focusing in particular on the isolation and identification of physiological substrates explaining their implication in biotic or abiotic stress responses. In particular, we are interested in GSTs regulated during poplar leaf infection by the rust fungus Melampsora larici-populina or regulated during iron starvation in A. thaliana.
The strategies used are to perform pull-down and affinity chromatographies, inhibition kinetics and thermal denaturation combining fluorescent probes and model ligands or plant extracts that are eventually separated by liquid ichromatography. We will not only focus on the previously characterized Cys-GSTs (lambda GSTs and glutathionyl hydroquinone reductases) but also on less explored or newly identified GST families. The nature (ligandin vs catalytic transformation) and the strength of the interactions will be validated by measuring binding constants using glutathionylated and non-glutathionylated molecules using biophycal approaches (ITC, Switchsense, fluorimetry). In parallel, the structure of GST alone or in complex with their ligand/substrate will be tentatively solved. Overall, we expect to decipher new intermediary steps of xenobiotic detoxification or of metabolic pathways requiring glutathione-conjugating or transport activities. New research questions have emerged recently from the observation that tau GST are able to bind porphyrins and hemes b in particular.
Collaborators: C. Didierjean (Univ. Lorraine), C. Dubos (BPMP, Montpellier), S. Lemaire (CNRS – Sorbonne Université)
Grants
-2019-2022 : Projet ANR-2018: MITOGLU: Dissecting the molecular interactions of mitochondrial glutaredoxin S15 in plants (coordinators N. Rouhier/A. Meyer)
-2018-2021 : Projet Lorraine Université Excellence, PEPS Mirabelle + : FeSprot: Maturation and structure-function analyses of iron-sulfur proteins (coordinator N. Rouhier)
– 2018-2021: Projet ANR 2017: MOBIFER: Dynamics of coumarin secretion by plant roots into the soil in the context of iron nutrition (coordinator C. Dubos)
– 2014-2017: Projet ANR 2013: Fe-S traffic: The cellular trafficking of iron-sulfur (Fe-S) clusters in plants (coordinator N. Rouhier)
– 2011-2013: Projet ANR 2010: FIRES: Functions of glutaredoxins in Iron and REdox sensing and Signaling in plants (coordinator N. Rouhier)
– 2009-2011: Projet INRA/FORMAS: The role of organellar glutaredoxins in iron-sulfur cluster biogenesis in plants: an interactomic study for the identification of protein partners (G. Wingsle (Umea Plant Science center, sweden)/ coordinator N. Rouhier)
– 2008-2010: Projet ANR Jeune Chercheur: The role of organellar CGFS containing glutaredoxins in iron-sulfur clusters assembly and biogenesis in plants (coordinator N. Rouhier)
– 2006-2008: Projet ANR, Réseau de Génomique végétale-Genoplante 2010: GNP05010P: Repair mechanisms of oxidized proteins in chloroplasts of Arabidopsis thaliana (coordinator Pascal Rey)
Publications
Publications
The plant atypical thioredoxins DCC1 possess chaperone activity.13th International Conference for Plant Mitochondrial Biology 2024, May 2024, Saint Malo, France
Communication dans un congrès
hal-04628771
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Characterization of the chloroplastic NFU1 and HCF101, two [4Fe-4S] cluster transfer proteins of the sulfur mobilization (SUF) machinery in Chlamydomonas reinhardtii.Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting., Jun 2024, Winston Salem, United States
Communication dans un congrès
hal-04628764
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Assembly and trafficking of iron sulfur clusters by NFU proteins in plastids is essential for photosynthesis.Enzymes & Photosynthesis- Key for a green and sustainable future. Japanese-European Symposium, May 2024, Osaka, Japan
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hal-04628782
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The plant DCC1 are atypical thioredoxins with a holdase activityPlant and Human Sulfur Biology Meeting, Nicolas Rouhier; Jérémy Couturier, Sep 2024, Pont-à-Mousson, France
Communication dans un congrès
hal-04991152
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Monitoring intracellular dynamics of cysteine with the genetically-encoded biosensor CyReBGDR NADPH Oxydase-Stress Oxydant & Club Oxydase, Nov 2023, Orsay, France
Communication dans un congrès
hal-04300881
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Roles of glutaredoxin S15 and BOLA4 in the maturation of iron-sulfur proteins in mitochondria.Congrès du Groupe Français de Bioénergétique, Sep 2023, Bédoin, France
Communication dans un congrès
hal-04628793
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Monitoring intracellular dynamics of cysteine with the genetically-encoded biosensor CyReBCompartmentalized redox biology, meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2023, Düsseldorf, Germany
Communication dans un congrès
hal-04300871
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Are Trx-like 3 and 4 new players in the thiol-based redox metabolism in the chloroplast ?Compartmentalized redox biology, Jan Riemer & Carsten Berndt, Sep 2023, Düsseldorf, Germany
Communication dans un congrès
hal-04229620
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The critical role of glutaredoxin S15 for the maturation of iron-sulfur proteins in mitochondria.Workshop “Metalloenzymes – cofactor assembly, catalytic mechanism and redox partners”., Nov 2022, Bochum, Germany
Communication dans un congrès
hal-04628800
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Role of the chloroplastic iron-sulfur transfer protein NFU1 in the microalga Chlamydomonas reinhardtiiBrussels Institute for Advanced Studies - Photosynthesis and primary production, Christian Hermans, Feb 2022, e-conference, Belgium
Communication dans un congrès
hal-04176818
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NAD(P)H cofactor electroenzymatic regeneration for selective biocatalytic reductions in a hybrid flow reactor73rd Annual Meeting of the International Society of Electrochemistry, Sep 2022, On Line, Switzerland
Communication dans un congrès
hal-04728428
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Rhodanese domain-containing sulfurtransferase family: a yet mysterious component of sulfur trafficking in plants12th International Plant Sulfur Workshop, Jul 2022, London, Canada
Communication dans un congrès
hal-03775204
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The chloroplastic iron-sulfur transfer protein NFU1 is required for chlorophyll synthesis in the dark and fermentation in the green microalga Chlamydomonas reinhardtii.Iron Sulfur Proteins : Biogenesis, Regulation and Function meeting., Sep 2022, Sainte-maxime, France
Communication dans un congrès
hal-04628802
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3-mercaptopyruvate sulfurtransferases: new actors in sulfur trafficking and H2S mediated-redox signalling in plants?Redox Biology Congress 2022 Oxidative stress, redox biology and antioxidants from plants to humans, Aug 2022, Ghent, Belgium
Communication dans un congrès
hal-03775274
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Maturation of iron-sulfur proteins in chloroplasts: focus on the NFU transfer proteinsSPS “Symposium on redox regulation and carbon-nitrogen interactions in plants. A tribute to Pierre Gadal”, Jun 2022, Gif sur Yvette, France
Communication dans un congrès
hal-03781860
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The chloroplastic iron-sulfur transfer protein NFU1 is required for chlorophyll synthesis in the dark and fermentation in the green microalga Chlamydomonas reinhardtiiIron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Sep 2022, Sainte Maxime, France
Communication dans un congrès
hal-03781853
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Redox properties of the plant atypical thioredoxin DCC1.Redox Biology Congress, Aug 2022, Ghent, Belgium
Communication dans un congrès
hal-03781876
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Deciphering the biochemical relationships between cysteine desulfurase and sulfurtransferase protein families in sulfur trafficking12th International Plant Sulfur Workshop, Jul 2022, London, Canada
Communication dans un congrès
hal-03775257
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Gene atlas of iron-containing proteins (ironOME) in Arabidopsis thalianaIron Nutrition and Interactions in Plants, Jul 2022, Reims, France
Communication dans un congrès
hal-04176793
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Identification of client proteins of the chloroplastic iron-sulfur transfer protein NFU2 in Arabidopsis thalianaJournées du PROTEOME VERT, INRAE, Jun 2022, Gif sur Yvette, France
Communication dans un congrès
hal-04766719
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The critical role of glutaredoxin S15 for the maturation of iron-sulfur proteins in mitochondria.12th International Conference for Plant Mitochondrial Biology 2022, May 2022, Malmö, Sweden
Communication dans un congrès
hal-03705989
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Towards the identification of novel metal- or thiol-based regulated proteins in plantsInternational Symposium of the DFG Priority Program 1710 Dynamics of Thiol-based Redox Switches in Cellular Physiology, Mar 2021, e-conference, Germany
Communication dans un congrès
hal-03197988
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Role of the chloroplastic NFU3 iron-sulfur transfer protein in the microalga Chlamydomonas reinhardtii.International Bioenergy and environment congress, Feb 2021, e-conference, France
Communication dans un congrès
hal-03197977
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The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in plant mitochondria.Congrès du Groupe Français de Bioénergétique, Sep 2021, online seminars, France
Communication dans un congrès
hal-03705957
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The role of chloroplastic NFU in the green alga Chlamydomonas reinhardtiiOnline seminar series on “Iron-sulfur protein biogenesis 2021”, Jun 2021, e-seminar, France
Communication dans un congrès
hal-03298704
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Maturation of Fe-S proteins in plant organelles.12th congress of the French Society of Plant Biology, Jul 2019, Strasbourg, France
Communication dans un congrès
hal-03197997
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Are the ubiquitous mitochondrial glutaredoxin-like proteins of 12 kDa (MGP12) true glutaredoxins?Thiol-based switches and redox regulation – from microbes to men, Sep 2019, San Feliu de Guixols, Spain
Communication dans un congrès
hal-03197996
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Sulfur trafficking and H2S-mediated redox signaling in plants: roles of Arabidopsis 3-MP sulfurtransferasesSPP1710 Conference Thiol-based switches and redox regulation – from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain
Communication dans un congrès
hal-03000210
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The maturation of Fe-S proteins in chloroplasts and mitochondriaJacques Monod conference “Retrograde signalling from endosymbiotic organelles”, Oct 2018, Roscoff, France
Communication dans un congrès
hal-02955303
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Elucidating the function of the chloroplastic glutaredoxin S16 of Arabidopsis thaliana.12th Congress of the International Plant Molecular Biology, Aug 2018, Montpellier, France
Communication dans un congrès
hal-03198404
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Deciphering the biochemical properties of bacterial rhodanese fusion proteins.Fourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany
Communication dans un congrès
hal-03000211
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Dynamics of coumarin secretion by plant roots into the soil to improve iron nutritionXVIII International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan
Communication dans un congrès
hal-02952560
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Functional characterization of mitochondrial NFU proteins in plants8th International Conference on Iron-Sulfur Cluster Biogenesis and Regulation, May 2018, Madison, United States
Communication dans un congrès
hal-02955576
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Who are the partners of mitochondrial Arabidopsis thaliana GRXS15 in trafficking of iron-sulfur clusters?Redox meeting and Conversation, Mar 2017, Vandoeuvre Lès Nancy, France
Communication dans un congrès
hal-04176965
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Arabidopsis thaliana GRXS16, a multifaceted glutaredoxin in chloroplasts.3rd Redox Biology Symposium, Redox regulation: Historical background and future developments, Mar 2017, Nancy, France
Communication dans un congrès
hal-03000213
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How are Fe-S cofactors and proteins assembled in plant cells? focus on NFUs, involved in the late steps of the maturation process in organellesDays of the French society of photosynthesis, Jun 2017, Paris, France
Communication dans un congrès
hal-02952587
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The chloroplastic glutaredoxin S16: a plant-specific, two-domain protein with multiple biochemical properties.EMBO Conference on Redox Biology, Jul 2017, Moscou, Russia
Communication dans un congrès
hal-03198418
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Deciphering the late-steps of FeS cluster assembly in plant organelles: focus on NFUs.Kick off meeting of the COST action CA15133 FeSBioNET “The biogenesis of iron-sulfur proteins: from cellular biology to molecular aspects”., Sep 2016, Patras, Greece
Communication dans un congrès
hal-03198428
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Deciphering the molecular mechanisms of sulfur trafficking in plants.hird meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Jul 2016, Düsseldorf, Germany
Communication dans un congrès
hal-03000214
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Exploring the partners of late-acting Fe-S transfer proteins belonging to the mitochondrial ISC machinery in Arabidopsis thaliana.Labex Mitocross 2015: mitochondria at the crossroad, Sep 2015, Strasbourg, France
Communication dans un congrès
hal-03198462
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Structure-function analysis of cysteine-containing glutathione transferases (GSTs) from Populus trichocarpaThiol-based redox switches in life sciences, EMBO-ESF meeting, Sep 2015, san feliu de guixols, Spain
Communication dans un congrès
hal-03198459
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The highly expanded thioredoxin and glutaredoxin classes in plants: specificities in the regeneration systems of enzymes using a sulfenic acid chemistryTheoretical and Practical Course “Redox Chemistry and Biology of Thiols” and associated symposium, Mar 2015, Montevideo, Uruguay
Communication dans un congrès
hal-03198450
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Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexesIUBMB Symposium FeS 2015 - Iron Sulfur Cluster Biogenesis and Regulation, Jun 2015, Bergamo, Italy
Communication dans un congrès
hal-03000372
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Plastidial Nfu2 and Nfu3 are crucial for a proper root and shoot development in Arabidopsis thaliana7th International Conference on Iron-Sulfur Cluster Biogenesis and Regulation., Jun 2015, bergame, Italy
Communication dans un congrès
hal-03198457
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The multiple facets of glutaredoxins: one fold, several partners and functions12th International Conference on Reactive Oxygen and Nitrogen Species in Plants: from model systems to field, Jun 2015, verone, Italy
Communication dans un congrès
hal-03198452
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Structure-function analyses of plant glutathione transferases with catalytic cysteine residuesTheoretical and Practical Course “Redox Chemistry and Biology of Thiols” and associated symposium., Mar 2015, montevideo, Uruguay
Communication dans un congrès
hal-03198451
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Plastidial glutaredoxins: glutathione-dependent enzymes involved in detoxification, redox signalling and iron-sulfur cluster biogenesis.9th International Workshop - Sulfur Metabolism in Plants., Apr 2014, freiburg, Germany
Communication dans un congrès
hal-03198438
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Biochemical, spectroscopic and structural insights into glutaredoxins and their multiple types of interactions with BolAs2nd Symposium of the DFG Priority Program SPP 1710 “Dynamics of Thiol-based Redox Switches in Cellular Physiology”, Sep 2014, Heidelberg, Germany
Communication dans un congrès
hal-03198444
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Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexes.IJB Symposium «Molecular Sciences, Facing Up to Major Societal Challenges»., Oct 2014, Nancy, France
Communication dans un congrès
hal-03198448
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Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexes.Gordon research conference “Thiol-based redox regulation and signalling”., Jul 2014, girone, Spain
Communication dans un congrès
hal-03198441
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Monothiol glutaredoxin and BolA in plants: two proteins with multiple interaction modes.9th International Workshop on Sulfur Metabolism in Plants: Molecular Physiology and Ecophysiology of Sulfur, Apr 2014, Freiburg, Germany
Communication dans un congrès
hal-03000377
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Genomics, transcriptomics and effectoromics of the poplar leaf rust fungus <em>Melampsora larici-populina</em>IUFRO Joint Meeting on Genetics of Five-Needle Pines and Rusts in Mountain Landscapes, Jun 2014, Fort Collins, United States
Communication dans un congrès
hal-02801804
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Investigation of the role of glutaredoxins and NFU proteins for the plastidial iron-sulfur cluster assembly machinary5th International Meeting of the Institute of Metals in Biology of Grenoble (IMBG)/Metal Homeostasis, Sep 2012, Autrans, France
Communication dans un congrès
hal-02952861
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Les apports de la génomique pour l’étude des familles multigéniques : exemple des thaumatin-like proteins (TLPs) chez le peuplierSéminaire de l’Ecole Doctorale RP2E. Biodiversité, Ingénierie écologique, Jan 2011, Vandoeuvre-lès-Nancy, France. 8 p
Communication dans un congrès
hal-02746248
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Characterization of thioredoxin-like 3, a conserved protein in the green lineagePlant Oxygen Group, May 2024, Juan-les-Pins / Antibes, France
Poster de conférence
hal-04602106
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Toward the characterisation of an unexplored class of glutaredoxins in photosynthetic eucaryotesVillage A2F, Jun 2024, Vandoeuvre -les-Nancy, France
Poster de conférence
hal-04666604
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Biochemical characterization of thioredoxin-related protein Clot/TRP14 of Populus trichocarpaCompartmentalized redox biology, meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2023, Düsseldorf, Germany
Poster de conférence
hal-04300914
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Development of a genetically-encoded biosensor to monitor an H2S-related metaboliteSociety for Redox Biology and Medicine (SfRBM) 30th Annual Conference and Society for Free Radical Research International (SFRRI) 21st Biennial Congress, Nov 2023, Punta del Este, Uruguay, Uruguay
Poster de conférence
hal-04302037
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The complex redox regulation of the SAL1 phosphatase present in plant organellesThiol oxidation in biology: Biochemical mechanisms to physiological outcomes, Oct 2022, sant feliu de guixols, Spain
Poster de conférence
hal-03820252
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Maturation of iron-sulfur-containing subunits of respiratory complex I in plantsIron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Sep 2022, Sainte Maxime, France
Poster de conférence
hal-03781848
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Exploiting a bacterial cysteine desulfurase-sulfurtransferase fusion to develop a roGFP2-based biosensor for free cysteineRedox Biology Congress 2022 Oxidative stress, redox biology and antioxidants from plants to humans, Aug 2022, Ghent, Belgium
Poster de conférence
hal-03775303
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Iron-sulfur cluster formation and transfer using plant mitochondrial glutaredoxin-BOLA complexes.Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting., Sep 2022, Sainte-maxime, France
Poster de conférence
hal-03820255
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Sulfurtransferase-mediated formation of H2S and persulfidation in Arabidopsis thalianaBotanik-Tagung International Conference of the German Society for Plant Sciences, Aug 2022, Bonn, Germany
Poster de conférence
hal-03775320
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Unravelling the role of an atypical mitochondrial ferredoxin from Arabidopsis thaliana.Congrès du Groupe Français de Bioénergétique, Sep 2021, online seminar, France
Poster de conférence
hal-03705964
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Integration of the chloroplastic iron-sulfur transfer protein NFU1 from Chlamydomonas reinhardtii in chlorophyll metabolism in the darkSfphi2021 : Journées de la Société Française de Photosynthèse, Apr 2021, online, France
Poster de conférence
hal-03298609
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Identification of client iron-sulfur proteins of the chloroplastic NFU3 transfer protein from Chlamydomonas reinhardtiiInternational Bioenergy and environment congress, Feb 2021, online, France
Poster de conférence
hal-03298613
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Identification of client iron-sulfur proteins of the chloroplastic NFU3 transfer protein from Chlamydomonas reinhardtii.International Bioenergy and environment congress, Feb 2021, online seminar, France
Poster de conférence
hal-03707813
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Shedding light on a H2S generating pathway in ArabidopsisS-Bio 2021 Glucosinolate Joint meeting for plant and human sulfur biology and glucosinolates, Sep 2021, Seville, Spain
Poster de conférence
hal-03358437
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Investigation of cellular interactions and biochemical characterization of the chloroplastic NFU1 transfer protein from Chlamydomonas reinhardtii.Chlamy 2020+1. 19th International Conference on the Cell and Molecular Biology of Chlamydomonas., Aug 2021, Six-Fours-les-Plages, France
Poster de conférence
hal-03707828
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Deciphering the biochemical relationships between cysteine desulfurase and sulfurtransferase protein families in sulfur traffickingS-Bio 2021 Glucosinolate Joint meeting for plant and human sulfur biology and glucosinolates, Sep 2021, Seville, Spain
Poster de conférence
hal-03358427
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Developing redox biosensors for sulfur compoundsSPP1710 Conference Thiol-based switches and redox regulation – from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain
Poster de conférence
hal-03000388
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Does Arabidopsis sulfurtransferase 18 participate to sulfur trafficking?Thiol-based switches and redox regulation - from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain
Poster de conférence
hal-02991609
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The α-amylase 3 of Arabidopsis thaliana is protected by S-glutathionylation in vitro114° Congresso della Società Botanica Italiana onlus, VI INTERNATIONAL PLANT SCIENCE CONFERENCE (IPSC)., Sep 2019, padova, Italy
Poster de conférence
hal-03298595
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Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition12ème Congrès de la Société Française de Biologie Végétale SFBV, Jul 2019, Strasbourg, France
Poster de conférence
hal-02951139
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Decrypting the chloroplastic [Fe-S] cluster assembly machineryXVIII International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan
Poster de conférence
hal-02951521
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Deciphering the biochemical properties of plastidial GRXS14 and mitochondrial GRXS15 isoforms of Arabidopsis thalianaFourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany
Poster de conférence
hal-03158701
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Dynamics of coumarin secretion by plant roots into the soil to improve iron nutritionXVIII International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan
Poster de conférence
hal-02951465
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Decrypting the chloroplastic [fe-s] cluster assembly machinery12th Congress of the International Plant Molecular Biology, Aug 2018, Montpellier, France. , 2018
Poster de conférence
hal-01869861
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Functional characterization of Arabidopsis BolA proteins in iron-sulfur center transfer pathways and in iron homeostasis12th International Plant Molecular Biology Congress, Aug 2018, Montpellier, France
Poster de conférence
hal-02951237
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Deciphering the biochemical properties of a bacterial ferredoxin-thioredoxin reductase-rhodanese fusion proteinFourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany
Poster de conférence
hal-03000393
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Sulfur trafficking and H2S mediated-redox signalling in plants: Biochemical characterization of Arabidopsis thaliana 3-MP sulfurtransferases12th Congress of the International Plant Molecular Biology (IPMB), Aug 2018, Montpellier, France
Poster de conférence
hal-03158693
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Seeking the role of the plastidial NFU1 transfer protein in Arabidopsis thaliana.Iron-Sulfur Proteins-Biogenesis, Regulation and Function 39th Steenbock Symposium, May 2018, Madison, United States
Poster de conférence
hal-03158684
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Mitochondrial Fe-S cluster assembly in Arabidopsis thaliana: In vitro characterization of the properties and role of GRX, ISCA and NFU proteinsIron-Sulfur Proteins-Biogenesis, Regulation and Function 39th Steenbock Symposium, May 2018, Madison, United States
Poster de conférence
hal-03158679
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Biochemical characterization of glutathione transferases potentially involved in the metabolism of coumarins in Arabidopsis thaliana during iron deficiencyJacques Monod conference “Retrograde signalling from endosymbiotic organelles", Oct 2018, Roscoff, France
Poster de conférence
hal-03298648
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Biochemical characterization of glutathione transferases potentially involved in the metabolism of coumarins in Arabidopsis thaliana during iron deficiencyMeeting of the SPP1710/Study group redox biology of the GBM, Sep 2018, Berlin, Germany
Poster de conférence
hal-03298653
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MIA40 of Arabidopsis thaliana is essential for the optimal in vitro oxidation of TIM and COX proteinsFourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany
Poster de conférence
hal-03158695
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Mia40 is not just a “poor man’s thioredoxin”Thiol oxidation in toxicity and signalling., Sep 2017, Sant Feliu de Guixols, Spain
Poster de conférence
hal-03298589
v1
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The cellular maturation of iron-sulfur proteins in plants1st FrenchBIC summer school, Methods for studying metals in biology, Sep 2017, Carry-le-Rouet, France
Poster de conférence
hal-03158671
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Towards the roles of Arabidopsis plastidial CGFS glutaredoxins: involvement of GRXS14 in the maintenance of chlorophyll content under specific physiological situations“Thiol oxidation in toxicity and signalling” Workshop, Sep 2017, Sant Feliu de Guixols, Spain
Poster de conférence
hal-03158359
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Comparison of the redox properties of plant and yeast MIA40/ERV1 couples“Thiol oxidation in toxicity and signalling” Workshop, Sep 2017, Sant Feliu de Guixols, Spain
Poster de conférence
hal-03158362
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Deciphering the biochemical properties of plant sulfurtransferases to dissect cellular sulfur trafficking mechanisms.“Thiol oxidation in toxicity and signalling” Workshop, Sep 2017, Sant Feliu de Guixols, Spain
Poster de conférence
hal-03158365
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Biochemical characterization of glutathione transferases potentially involved in the metabolism of coumarins in Arabidopsis thaliana during iron deficiencyThiol oxidation in toxicity and signalling, Sep 2017, Sant Feliu de Guixols, Spain
Poster de conférence
hal-02951534
v1
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The cellular maturation of iron-sulfur proteins in plants1st FeSBioNet Training School, Jun 2017, Lisbonne, Portugal
Poster de conférence
hal-03158357
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Arabidopsis thaliana NFU proteins participate in the final steps of the maturation of mitochondrial iron-sulfur proteinsGordon Research Conferences on Thiol-Based Redox Regulation & Signaling, Jul 2016, Barga, Italy
Poster de conférence
hal-02951566
v1
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Towards the function of the chloroplastic Arabidopsis thaliana glutaredoxin S163rd meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Jul 2016, Düsseldorf, Germany
Poster de conférence
hal-03158354
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Mitochondrial Arabidopsis thaliana NFU transfer proteins: cooperation with ISCA proteins to deliver [4Fe-4S] cluster to specific apo-targetsXVIII International Symposium on Iron Nutrition and Interactions in Plants, May 2016, Madrid, Spain. 2016
Poster de conférence
hal-01595219
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Structural characterization of BolA-metal complexes through Sinorhizobium meliloti YrbACongrès de l’Association Française de Cristallographie (AFC), Jul 2016, Marseille, France
Poster de conférence
hal-03158348
v1
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Identification of mitochondrial ferredoxin partners from Arabidospis thaliana leaves18th International Symposium on Iron Nutrition and Interaction in Plants, Jun 2016, Madrid, Spain
Poster de conférence
hal-03158345
v1
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Exploring the functions and partners of mitochondrial ISCA transfer proteins from A. thalianaIUBMB Symposium FeS 2015 - Iron Sulfur Cluster Biogenesis and Regulation, Jun 2015, Bergamo, Italy
Poster de conférence
hal-03158314
v1
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Structural investigation of poplar dehydroascorbate reductase by NMR spectroscopy2nd Redox meeting, 2015, Vandoeuvre lès Nancy, France
Poster de conférence
hal-04482685
v1
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Deciphering the functions and partners of mitochondrial ferredoxins from Arabidopsis thalianaChemistry & Biology of Iron-Sulfur Clusters 6th International IMBG Meeting & Advanced Courses, Sep 2015, Villard de Lans, France
Poster de conférence
hal-03158320
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Exploring the functions and partners of mitochondrial ISCA transfer proteins from A. thalianaMosbacher Kolloquium - Metals in Biology: Cellular functions and diseases, Mar 2015, Mosbach, Germany
Poster de conférence
hal-03158301
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Diverse structural forms involving BolA proteinsMosbacher Kolloquium - Metals in Biology: Cellular functions and diseases, Mar 2015, Mosbach, Germany
Poster de conférence
hal-03158306
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Arabidopsis thaliana plastidial Nfu2 and Nfu3 are required for the maturation of [4Fe-4S] clusters into photosystem I7th International Conference on Iron-Sulfur Cluster Biogenesis and Regulation, May 2013, South Carolina, United States
Poster de conférence
hal-03158292
v1
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NMR study of Arabidopsis thaliana BolA2 : structure, dynamics and interaction with glutaredoxinsEuromar Magnetic Resonance Conference, Jun 2013, Hersonissos, Greece
Poster de conférence
hal-04482665
v1
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Arabidopsis thaliana plastidial Nfu2 and Nfu3 are required for the maturation of [4Fe-4S] clusters into photosystem7th International Conference on the Biogenesis of Iron Sulphur Proteins, May 2013, Columbia, United States. 2013
Poster de conférence
hal-01594163
v1
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Study of Arabidopsis thaliana BolA2 : Structure, Dynamics and Interaction with GlutaredoxinsXXVth International Conference on Magnetic Resonance in Biological Systems, Aug 2012, Lyon, France
Poster de conférence
hal-03158262
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NMR study of Arabidopsis thaliana BolA2 : structure, dynamics and interaction with glutaredoxinsXXVth International Conference on Magnetic Resonance in Biological Systems, Aug 2012, Lyon, France
Poster de conférence
hal-04482637
v1
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Clusters fer-soufre : rôle des glutarédoxines dans la machinerieGTBio, Apr 2012, Montpellier, France
Poster de conférence
hal-04482628
v1
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Roles of glutaredoxins in iron-sulfur cluster biogenesis and intracellular iron sensing in plants5th International Meeting and Second Advanced Courses of the IMBG: Metals Homeostasis, Sep 2012, Autrans, France
Poster de conférence
hal-03158283
v1
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Peroxide detoxification systems in poplarPlant Abiotic Stress tolerance II. Section Plant Response to Oxidative Stress, Feb 2012, Vienna, Austria
Poster de conférence
hal-03158255
v1
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Structure/function analysis of plant BolAs, deciphering their interaction with glutaredoxinsBiogenesis of iron sulphur proteins and regulatory functions, Aug 2011, Cambridge, United Kingdom
Poster de conférence
hal-03158225
v1
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Structural and functional characterization of a plastidial poplar glutaredoxin with an atypical WCSYS active site36ème Forum des Jeunes Chercheurs de la Société Française de Biochimie et de Biologie Moléculaire, Aug 2009, Nancy, France
Poster de conférence
hal-03158204
v1
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Structural and functional characterization of a plastidial poplar glutaredoxin with an atypical WCSYS active siteGlutathione International Symposium: Glutathione and related thiols in microorganisms and plants, Aug 2008, Nancy, France
Poster de conférence
hal-03158072
v1
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Structural and functional characterization of a plastidial poplar glutaredoxin with an atypical WCSYS active siteGordon Research Conferences : Thio-Based Redox Regulation & Signalling, May 2008, Barga, Italy
Poster de conférence
hal-03158003
v1
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Structural diversity and specificities within the ferredoxin disulfide/thioredoxin reductase familyJohn Wiley & Sons. Encyclopedia of Inorganic and bioinorganic chemistry, 2024, 9781119951438. ⟨10.1002/9781119951438.eibc2868⟩
Chapitre d'ouvrage
hal-04628824
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The mitochondrion: from genome to proteomeThe Chlamydomonas Sourcebook, Elsevier, pp.369-412, 2022, ⟨10.1016/B978-0-12-821430-5.00011-0⟩
Chapitre d'ouvrage
hal-04176659
v1
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Thiol-based redox control in chloroplastsRedox Chemistry and Biology of Thiols, Elsevier, pp.507-532, 2022, ⟨10.1016/B978-0-323-90219-9.00023-6⟩
Chapitre d'ouvrage
hal-03706004
v1
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News on the redox front—A follow-up of ABR volume 52: Oxidative stress and redox regulation in plantsAdvances in Botanical Research, 100, pp.355-378, 2021, ⟨10.1016/bs.abr.2021.01.010⟩
Chapitre d'ouvrage
hal-03229149
v1
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FeS Cluster Assembly: Role of Monothiol Grxs and Nfu ProteinsEncyclopedia of Inorganic and Bioinorganic Chemistry, John Wiley & Sons, Ltd, 2017, ⟨10.1002/9781119951438.eibc2470⟩
Chapitre d'ouvrage
hal-02945095
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In Vitro Alkylation Methods for Assessing the Protein Redox StatePhotorespiration : Methods and Protocols, pp.51-64, 2017, ⟨10.1007/978-1-4939-7225-8_4⟩
Chapitre d'ouvrage
hal-02913009
v1
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Redox HomeostasisUrsula Jakob; Dan Reichmann. Oxidative Stress and Redox Regulation, Springer Netherlands, pp.59-84, 2013, 978-94-007-5786-8. ⟨10.1007/978-94-007-5787-5_3⟩
Chapitre d'ouvrage
hal-01940943
v1
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Redox Regulation in Plants: Glutathione and “Redoxin” Related FamiliesUrsula Jakob; Dan Reichmann. Oxidative Stress and Redox Regulation, Springer Netherlands, pp.213-231, 2013, 978-94-007-5786-8. ⟨10.1007/978-94-007-5787-5_8⟩
Chapitre d'ouvrage
hal-01940924
v1
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Glutaredoxin: The Missing Link Between Thiol-Disulfide Oxidoreductases and Iron Sulfur EnzymesAdvances in Botanical Research: Oxidative stress and redox regulations in plants, 52, Elsevier, 2009, Advances in Botanical Research, 978-0-12-378622-7. ⟨10.1016/S0065-2296(10)52013-5⟩
Chapitre d'ouvrage
hal-02823131
v1
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Régulation rédox glutathion dépendante chez le peuplier : Isolement et caractérisation de la glutarédoxine2000, 22p
Autre publication scientifique
hal-01600417
v1
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The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in mitochondria2022
Pré-publication, Document de travail
hal-03857817
v2
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Aspects fonctionnels et structuraux des systèmes thiorédoxine et glutarédoxine de peuplier et de leurs enzymes cibles, les peroxyrédoxines et les méthionine sulfoxyde réductases de type ABiologie végétale. Université Henri Poincaré - Nancy 1, 2003. Français. ⟨NNT : 2003NAN10104⟩
Thèse
tel-01748118
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