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NICOLAS ROUHIER

280
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Presentation

I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins. After a PhD in plant physiology, molecular biology and biochemistry, I have been recruited in 2004 as an assistant professor at the Université of Nancy, obtained an habilitation (HDR) in 2007 before gettting a professor position in 2011 at the newly created Université of Lorraine. For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with *Populus trichocarpa* and *Arabidopsis thaliana* as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles. - **Redox post-translational modifications of proteins** In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX. The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations. **Collaborators: C. Didierjean (Univ. Lorraine), JM. Herrmann (Kaiserslautern), S. Lemaire (CNRS – Sorbonne Université), A. Meyer (Bonn), B. Morgan (Saarbrücken), P. Rey (CEA Cadarache)** - **Molecular analysis of late steps of Fe-S cluster maturation in organelles** The general objective here is to understand the functioning of the Fe-S cluster assembly machineries in organelles and more precisely the molecular mechanisms controlling the second step, *i.e.* the trafficking of preformed Fe-S clusters from scaffold proteins (made in the first step) to final acceptors. After focusing for some time on the capacity of several plant GRXs to assemble \[2Fe-2S\] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. Particular emphasis is given to the maturation of the photosynthetic and respiratory electron transfer complexes. **Collaborators: J. Balk (JIC, Norwich), MK. Johnson (Athens, USA), O. Keech (Umea), A. Meyer (Bonn), U. Mühlenhoff (Marburg), C. Remacle (Liège), F. Vignols (BPMP Montpellier)** - **Structure-Function analysis of glutathione transferases** Glutathione transferases (GSTs) constitute large multigenic families of more than 50 genes in terrestrial plants. They have three major biochemical functions. Those having a serinyl residue in their catalytic site (Ser-GSTs) possess GSH-conjugation activity. This catalytic activity is often described as required for xenobiotic detoxification. Those having a cysteinyl residue in their catalytic site (Cys-GSTs) possess the ability to catalyze the reverse reaction, *i.e.*, the removal of glutathione from a number of structurally different heterocyclic molecules. The third function is the binding and/or transport of molecules/specialized metabolites without catalytic transformation, so-called ligandin function. It can be performed both by Ser-GSTs and Cys-GSTs. Although they are present in all kingdoms, the physiological functions of most plant GSTs remain elusive. The current work is centered on the structure-function analysis of specific classes of glutathione transferases, focusing in particular on the isolation and identification of physiological substrates explaining their implication in biotic or abiotic stress responses. In particular, we are interested in GSTs regulated during poplar leaf infection by the rust fungus *Melampsora larici-populina* or regulated during iron starvation in *A. thaliana*. The strategies used are to perform pull-down and affinity chromatographies, inhibition kinetics and thermal denaturation combining fluorescent probes and model ligands or plant extracts that are eventually separated by liquid ichromatography. We will not only focus on the previously characterized Cys-GSTs (lambda GSTs and glutathionyl hydroquinone reductases) but also on less explored or newly identified GST families. The nature (ligandin *vs* catalytic transformation) and the strength of the interactions will be validated by measuring binding constants using glutathionylated and non-glutathionylated molecules using biophycal approaches (ITC, Switchsense, fluorimetry). In parallel, the structure of GST alone or in complex with their ligand/substrate will be tentatively solved. Overall, we expect to decipher new intermediary steps of xenobiotic detoxification or of metabolic pathways requiring glutathione-conjugating or transport activities. New research questions have emerged recently from the observation that tau GST are able to bind porphyrins and hemes b in particular. **Collaborators: C. Didierjean (Univ. Lorraine)**, **C. Dubos (BPMP, Montpellier), S. Lemaire (CNRS – Sorbonne Université)** **Grants** -2019-2022 : **Projet ANR-2018**: MITOGLU: Dissecting the molecular interactions of mitochondrial glutaredoxin S15 in plants (coordinators N. Rouhier/A. Meyer) -2018-2021 : **Projet Lorraine Université Excellence**, PEPS Mirabelle + : FeSprot: Maturation and structure-function analyses of iron-sulfur proteins (coordinator N. Rouhier) – 2018-2021: **Projet ANR 2017**: MOBIFER: Dynamics of coumarin secretion by plant roots into the soil in the context of iron nutrition (coordinator C. Dubos) – 2014-2017: **Projet ANR 2013**: Fe-S traffic: The cellular trafficking of iron-sulfur (Fe-S) clusters in plants (coordinator N. Rouhier) – 2011-2013: **Projet ANR 2010**: FIRES: Functions of glutaredoxins in Iron and REdox sensing and Signaling in plants (coordinator N. Rouhier) – 2009-2011: **Projet INRA/FORMAS:** The role of organellar glutaredoxins in iron-sulfur cluster biogenesis in plants: an interactomic study for the identification of protein partners (G. Wingsle (Umea Plant Science center, sweden)/ coordinator N. Rouhier) – 2008-2010: **Projet ANR Jeune Chercheur**: The role of organellar CGFS containing glutaredoxins in iron-sulfur clusters assembly and biogenesis in plants (coordinator N. Rouhier) – 2006-2008: **Projet ANR, Réseau de Génomique végétale-Genoplante 2010**: GNP05010P: Repair mechanisms of oxidized proteins in chloroplasts of *Arabidopsis thaliana* (coordinator Pascal Rey)

Publications

Ferredoxins at the crossroads

Nicolas Rouhier
Nature Chemical Biology, 2023, 19 (2), pp.129-130. ⟨10.1038/s41589-022-01176-3⟩
Journal articles hal-04059912v1

Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis

Helene Röhricht , Jonathan Przybyla-Toscano , Joachim Forner , Clément Boussardon , Olivier Keech
Plant Physiology, 2023, 191 (4), pp.2170-2184. ⟨10.1093/plphys/kiad040⟩
Journal articles hal-04059909v1

Relationships between the Reversible Oxidation of the Single Cysteine Residue and the Physiological Function of the Mitochondrial Glutaredoxin S15 from Arabidopsis thaliana

Loïck Christ , Jérémy Couturier , Nicolas Rouhier
Antioxidants , 2023, 12 (1), pp.102. ⟨10.3390/antiox12010102⟩
Journal articles hal-04059914v1
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Maturation and Assembly of Iron-Sulfur Cluster-Containing Subunits in the Mitochondrial Complex I From Plants

Alicia López-López , Olivier Keech , Nicolas Rouhier
Frontiers in Plant Science, 2022, 13, pp.916948. ⟨10.3389/fpls.2022.916948⟩
Journal articles hal-03705940v1

The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18

Benjamin Selles , Anna Moseler , Damien Caubrière , Sheng-Kai Sun , Morgane Ziesel
Journal of Biological Chemistry, 2022, 298 (4), pp.101749. ⟨10.1016/j.jbc.2022.101749⟩
Journal articles hal-03583118v1
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Thiol Reductases in Deinococcus Bacteria and Roles in Stress Tolerance

Arjan de Groot , Laurence Blanchard , Nicolas Rouhier , Pascal Jean Del Rey
Antioxidants , 2022, 11 (3), pp.561. ⟨10.3390/antiox11030561⟩
Journal articles hal-03617589v1
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Redox regulation of enzymes involved in sulfate assimilation and in the synthesis of sulfur-containing amino acids and glutathione in plants

Linda de Bont , Natacha Donnay , Jérémy Couturier , Nicolas Rouhier
Frontiers in Plant Science, 2022, 13, pp.958490. ⟨10.3389/fpls.2022.958490⟩
Journal articles hal-03774111v1

Structural Insights into a Fusion Protein between a Glutaredoxin-like and a Ferredoxin-Disulfide Reductase Domain from an Extremophile Bacterium

Flavien Zannini , Sandrine Mathiot , Jérémy Couturier , Claude Didierjean , Nicolas Rouhier
Inorganics, 2022, 10 (2), pp.24. ⟨10.3390/inorganics10020024⟩
Journal articles hal-03610879v1
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Protein lipoylation in mitochondria requires Fe–S cluster assembly factors NFU4 and NFU5

Jonathan Przybyla-Toscano , Andrew E Maclean , Marina Franceschetti , Daniela Liebsch , Florence Vignols
Plant Physiology, 2022, 188 (2), pp.997-1013. ⟨10.1093/plphys/kiab501⟩
Journal articles hal-03435679v1

Glutaredoxins with iron-sulphur clusters in eukaryotes - Structure, function and impact on disease

Carsten Berndt , Loïck Christ , Nicolas Rouhier , Ulrich Mühlenhoff
Biochimica biophysica acta (BBA) - Bioenergetics, 2021, 1862 (1), pp.148317. ⟨10.1016/j.bbabio.2020.148317⟩
Journal articles hal-03304415v1

Gene atlas of iron‐containing proteins in Arabidopsis thaliana

Jonathan Przybyla‐toscano , Clément Boussardon , Simon Law , Nicolas Rouhier , Olivier Keech
Plant Journal, 2021, 106 (1), pp.258-274. ⟨10.1111/tpj.15154⟩
Journal articles hal-03304452v1

Coumarin accumulation and trafficking in Arabidopsis thaliana : a complex and dynamic process

Kevin Robe , Geneviève Conejero , Fei Gao , Linnka Lefebvre‐legendre , Elodie Sylvestre‐gonon
New Phytologist, 2021, 229 (4), pp.2062-2079. ⟨10.1111/nph.17090⟩
Journal articles hal-03450444v1
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Arabidopsis thaliana 3-mercaptopyruvate sulfurtransferases interact with and are protected by reducing systems

Anna Moseler , Tiphaine Dhalleine , Nicolas Rouhier , Jérémy Couturier
Journal of Biological Chemistry, 2021, pp.100429. ⟨10.1016/j.jbc.2021.100429⟩
Journal articles hal-03157547v1

Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae

Jonathan Przybyla-Toscano , Jérémy Couturier , Claire Remacle , Nicolas Rouhier
International Journal of Molecular Sciences, 2021, 22 (6), pp.3175. ⟨10.3390/ijms22063175⟩
Journal articles hal-03304524v1
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Atypical Iron-Sulfur Cluster Binding, Redox Activity and Structural Properties of Chlamydomonas reinhardtii Glutaredoxin 2

Thomas Roret , Bo Zhang , Anna Moseler , Tiphaine Dhalleine , Xing-Huang Gao
Antioxidants , 2021, 10 (5), pp.803. ⟨10.3390/antiox10050803⟩
Journal articles hal-03241937v1

Iron–sulfur proteins in plant mitochondria: roles and maturation

Jonathan Przybyla-Toscano , Loïck Christ , Olivier Keech , Nicolas Rouhier
Journal of Experimental Botany, 2021, 72 (6), pp.2014-2044. ⟨10.1093/jxb/eraa578⟩
Journal articles hal-03304521v1

A Redox-Sensitive Cysteine Is Required for PIN1At Function

Benjamin Selles , Tiphaine Dhalleine , Alexis Boutilliat , Nicolas Rouhier , Jérémy Couturier
Frontiers in Plant Science, 2021, 12, pp.735423. ⟨10.3389/fpls.2021.735423⟩
Journal articles hal-03541790v1
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[4Fe-4S] cluster trafficking mediated by Arabidopsis mitochondrial ISCA and NFU proteins

Tamanna Azam , Jonathan Przybyla-Toscano , Florence Vignols , Jérémy Couturier , Nicolas Rouhier
Journal of Biological Chemistry, 2020, 295 (52), pp.18367-18378. ⟨10.1074/jbc.RA120.015726⟩
Journal articles hal-03052839v1

Characterization of redox properties of FAD cofactor of Thermotoga maritima thioredoxin reductase

Benjaminas Valiauga , Nicolas Rouhier , Jean-Pierre Jacquot , Narimantas Čėnas
Chemija, 2020, 31 (3), pp.191-195. ⟨10.6001/chemija.v31i3.4293⟩
Journal articles hal-02945103v1
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The Arabidopsis Mitochondrial Glutaredoxin GRXS15 Provides [2Fe-2S] Clusters for ISCA-Mediated [4Fe-4S] Cluster Maturation

Tamanna Azam , Jonathan Przybyla-Toscano , Florence Vignols , Jérémy Couturier , Nicolas Rouhier
International Journal of Molecular Sciences, 2020, 21 (23), pp.9237. ⟨10.3390/ijms21239237⟩
Journal articles hal-03076877v1

Sinorhizobium meliloti YrbA binds divalent metal cations using two conserved histidines

Thomas Roret , Geneviève Alloing , Jean-Michel Girardet , Thomas Perrot , Tiphaine Dhalleine
Bioscience Reports, 2020, 40 (10), pp.51-64. ⟨10.1042/BSR20202956⟩
Journal articles hal-03000204v1

Novel insights into the diversity of the sulfurtransferase family in photosynthetic organisms with emphasis on oak

Anna Moseler , Benjamin Selles , Nicolas Rouhier , Jérémy Couturier
New Phytologist, 2020, 226 (4), pp.967-977. ⟨10.1111/nph.15870⟩
Journal articles hal-02545811v1
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A Global Proteomic Approach Sheds New Light on Potential Iron-Sulfur Client Proteins of the Chloroplastic Maturation Factor NFU3

Nathalie Berger , Florence Vignols , Brigitte Touraine , Maël Taupin-Broggini , Valérie Rofidal
International Journal of Molecular Sciences, 2020, 21 (21), ⟨10.3390/ijms21218121⟩
Journal articles hal-03052853v1
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Identification of client iron-sulfur proteins of the chloroplastic NFU2 transfer protein in Arabidopsis thaliana

Nathalie Berger , Florence Vignols , Jonathan Przybyla-Toscano , Mélanie Roland , Valerie Rofidal
Journal of Experimental Botany, inPress, 71 (14), pp.4171-4187. ⟨10.1093/jxb/eraa166⟩
Journal articles hal-02533200v1

Methylglyoxal Detoxification Revisited: Role of Glutathione Transferase in Model Cyanobacterium Synechocystis sp. Strain PCC 6803

Xavier Kammerscheit , Arnaud Hecker , Nicolas Rouhier , Franck Chauvat , Corinne Cassier-Chauvat
mBio, 2020, 11 (4), pp.1-13. ⟨10.1128/mBio.00882-20⟩
Journal articles hal-02928172v1

Is there a role for tau glutathione transferases in tetrapyrrole metabolism and retrograde signalling in plants?

Elodie Sylvestre-Gonon , Mathieu Schwartz , Jean-Michel Girardet , Arnaud Hecker , Nicolas Rouhier
Philosophical Transactions of the Royal Society B: Biological Sciences, 2020, 375 (1801), ⟨10.1098/rstb.2019.0404⟩
Journal articles hal-02890959v1
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The plastidial Arabidopsis thaliana NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins

Mélanie Roland , Jonathan Przybyla-Toscano , Florence Vignols , Nathalie Berger , Tamanna Azam
Journal of Biological Chemistry, 2020, 295 (6), pp.1727-1742. ⟨10.1074/jbc.RA119.011034⟩
Journal articles hal-02473677v1

Iron–sulfur protein NFU2 is required for branched-chain amino acid synthesis in Arabidopsis roots

Brigitte Touraine , Florence Vignols , Jonathan Przybyla-Toscano , Till Ischebeck , Tiphaine Dhalleine
Journal of Experimental Botany, 2019, 70 (6), pp.1875-1889. ⟨10.1093/jxb/erz050⟩
Journal articles hal-02095655v1
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Is There a Role for Glutaredoxins and BOLAs in the Perception of the Cellular Iron Status in Plants?

Pascal Rey , Maël Taupin-Broggini , Jérémy Couturier , Florence Vignols , Nicolas Rouhier
Frontiers in Plant Science, 2019, 10, pp.712. ⟨10.3389/fpls.2019.00712⟩
Journal articles hal-02571980v2
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The Thioredoxin-Regulated α-Amylase 3 of Arabidopsis thaliana Is a Target of S-Glutathionylation

Libero Gurrieri , Luca Distefano , Claudia Pirone , Daniel Horrer , David Seung
Frontiers in Plant Science, 2019, 10, ⟨10.3389/fpls.2019.00993⟩
Journal articles hal-02352493v1

Redox Homeostasis in Photosynthetic Organisms: Novel and Established Thiol-Based Molecular Mechanisms

Mirko Zaffagnini , Simona Fermani , Christophe Marchand , Alex Costa , Francesca Sparla
Antioxidants and Redox Signaling, 2019, 31 (3), pp.155-210. ⟨10.1089/ars.2018.7617⟩
Journal articles hal-02350148v1
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Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds

Karine de Guillen , Cécile Lorrain , Pascale Tsan , Philippe Barthe , Benjamin Petre
Scientific Reports, 2019, 9 (1), pp.18084. ⟨10.1038/s41598-019-53816-9⟩
Journal articles hal-02548559v1

Oxidative protein folding

Andreas J Meyer , Jan Riemer , Nicolas Rouhier
New Phytologist, 2019, 221 (3), pp.1230-1246. ⟨10.1111/nph.15436⟩
Journal articles hal-02154461v1

Rhodanese domain-containing sulfurtransferases

Benjamin Selles , Anna-Maria Moseler , Nicolas Rouhier , Jérémy Couturier
Journal of Experimental Botany, 2019, 70 (16), pp.4139-4154. ⟨10.1093/jxb/erz213⟩
Journal articles hal-02352485v1

The thioredoxin-mediated recycling of Arabidopsis thaliana GRXS16 relies on a conserved C-terminal cysteine

Flavien Zannini , Anna-Maria Moseler , Raphael Bchini , Tiphaine Lamant Dhalleine , Andreas J Meyer
Biochimica et Biophysica Acta (BBA) - General Subjects, 2019, 1863 (2), pp.426-436. ⟨10.1016/j.bbagen.2018.11.014⟩
Journal articles hal-02154449v1
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Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases.

Elodie Sylvestre-Gonon , Simon Law , Mathieu Schwartz , Kevin Robe , Olivier Keech
Frontiers in Plant Science, 2019, 10, pp.608. ⟨10.3389/fpls.2019.00608⟩
Journal articles hal-02139339v1
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Structural snapshots along the reaction mechanism of the atypical poplar thioredoxin-like2.1.

Kamel Chibani , Frederick Saul , Claude Didierjean , Nicolas Rouhier , Ahmed Haouz
FEBS Letters, 2018, 592 (6), pp.1030-1041. ⟨10.1002/1873-3468.13009⟩
Journal articles hal-01844909v1
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Function and maturation of the Fe-S center in dihydroxyacid dehydratase from Arabidopsis

Huanyao Gao , Tamanna Azam , Sajini Randeniya , Jérémy Couturier , Nicolas Rouhier
Journal of Biological Chemistry, 2018, 293 (12), pp.4422-4433. ⟨10.1074/jbc.RA117.001592⟩
Journal articles hal-01816913v1

Conserved functions of Arabidopsis mitochondrial late-acting maturation factors in the trafficking of iron‑sulfur clusters

Marta A. Uzarska , Jonathan Przybyla Toscano , Farah Spantgar , Flavien Zannini , Roland Lill
Biochimica et Biophysica Acta - Molecular Cell Research, 2018, 1865 (9), pp.1250-1259. ⟨10.1016/j.bbamcr.2018.06.003⟩
Journal articles hal-01893946v1
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Mitochondrial Arabidopsis thaliana TRXo Isoforms Bind an Iron–Sulfur Cluster and Reduce NFU Proteins In Vitro

Flavien Zannini , Thomas Roret , Jonathan Przybyla-Toscano , Tiphaine Dhalleine , Nicolas Rouhier
Antioxidants , 2018, 7 (10), pp.142. ⟨10.3390/antiox7100142⟩
Journal articles hal-02545816v1
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Oak genome reveals facets of long lifespan

Christophe Plomion , Jean-Marc Aury , Joelle Amselem , Thibault Leroy , Florent Murat
Nature Plants, 2018, 4 (7), pp.440-452. ⟨10.1038/s41477-018-0172-3⟩
Journal articles hal-01820559v1

Roles and maturation of iron–sulfur proteins in plastids

Jonathan Przybyla-Toscano , Mélanie Roland , Frederic Gaymard , Jérémy Couturier , Nicolas Rouhier
Journal of Biological Inorganic Chemistry, 2018, 23 (4), pp.545-566. ⟨10.1007/s00775-018-1532-1⟩
Journal articles hal-01690262v1

Covalent and Non-Covalent Associations Mediate MED28 Homo- Oligomerization

Jehad Shaikhali , Nicolas Rouhier , Arnaud Hecker , Kristoffer Brannstrom , Gunnar Wingsle
Journal of Plant Biochemistry & Physiology, 2017, 05 (02), ⟨10.4172/2329-9029.1000189⟩
Journal articles hal-02945082v1
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Atypical protein disulfide isomerases (PDI)

Benjamin Selles , Flavien Zannini , Jérémy Couturier , Jean-Pierre Jacquot , Nicolas Rouhier
PLoS ONE, 2017, 12 (3), pp.e0174753. ⟨10.1371/journal.pone.0174753⟩
Journal articles hal-01562315v1

The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations

Olivier Keech , Per Gardeström , Leszek A Kleczkowski , Nicolas Rouhier
Plant, Cell and Environment, 2017, 40 (4), pp.553-569. ⟨10.1111/pce.12713⟩
Journal articles hal-01573241v1

Post-translational modifications of Medicago truncatula glutathione peroxidase 1 induced by nitric oxide

Claude Castella , Ioanna Mirtziou , Aurélie Seassau , Alexandre Boscari , Françoise Montrichard
Nitric Oxide: Biology and Chemistry, 2017, 68, pp.125-136. ⟨10.1016/j.niox.2017.02.004⟩
Journal articles hal-01573246v1
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Involvement of Arabidopsis glutaredoxin S14 in the maintenance of chlorophyll content

Pascal Rey , Noëlle Becuwe , Sébastien Tourrette , Nicolas Rouhier
Plant, Cell and Environment, 2017, 40 (10), pp.2319-2332. ⟨10.1111/pce.13036⟩
Journal articles hal-01686869v1

Structural plasticity among glutathione transferase Phi members

Henri Pégeot , Sandrine Mathiot , Thomas Perrot , Frédéric Gense , Arnaud Hecker
FEBS Journal, 2017, 284 (15), pp.2442-2463. ⟨10.1111/febs.14138⟩
Journal articles hal-01593202v1
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Erv1 of Arabidopsis thaliana can directly oxidize mitochondrial intermembrane space proteins in the absence of redox-active Mia40.

Valentina Peleh , Flavien Zannini , Sandra Backes , Nicolas Rouhier , Johannes M Herrmann
BMC Biology, 2017, 15, pp.1-14. ⟨10.1186/s12915-017-0445-8⟩
Journal articles hal-02629175v1
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The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the Growth of the Leaf Rust Pathogen Melampsora larici-populina and Triggers Cell Culture Alkalinisation

Benjamin Petre , Arnaud Hecker , Hugo Germain , Pascale Tsan , Jan Sklenar
Frontiers in Plant Science, 2016, 7, pp.e1001732. ⟨10.3389/fpls.2016.00097⟩
Journal articles hal-01579575v1

Plastidic P2 glucose-6P dehydrogenase from poplar is modulated by thioredoxin m-type

Manuela Cardi , Mirko Zaffagnini , Alessia de Lillo , Daniela Castiglia , Kamel Chibani
Plant Science, 2016, 252, pp.257-266. ⟨10.1016/j.plantsci.2016.08.003⟩
Journal articles hal-01496820v1

Regulation of Differentiation of Nitrogen-Fixing Bacteria by Microsymbiont Targeting of Plant Thioredoxin s1.

Carolina Werner Ribeiro , Fabien Baldacci-Cresp , Olivier Pierre , Marie Larousse , Sofiane Benyamina
Current Biology - CB, 2016, 27 (2), pp.250-256. ⟨10.1016/j.cub.2016.11.013⟩
Journal articles hal-01439293v1
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Structural and functional characterization of tree proteins involved in redox regulation: a new frontier in forest science

Jean-Pierre Jacquot , Jérémy Couturier , Claude Didierjean , Éric Gelhaye , Mélanie Morel-Rouhier
Annals of Forest Science, 2016, 73 (1), pp.119-134. ⟨10.1007/s13595-014-0442-9⟩
Journal articles hal-01284229v1

Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa

Pierre-Alexandre Lallement , Thomas Roret , Pascale Tsan , José M Gualberto , Jean-Michel Girardet
Biochemical Journal, 2016, 473 (6), pp.717-31. ⟨10.1042/BJ20151147⟩
Journal articles hal-01287772v1

Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence

Daria Chrobok , Simon R. Law , Bastiaan Brouwer , Pernilla Lindén , Agnieszka Ziolkowska
Plant Physiology, 2016, 172 (4), pp.2132-2153. ⟨10.1104/pp.16.01463⟩
Journal articles hal-01494737v1

Biochemical and redox characterization of the mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein.

Jehad Shaikhali , Céline Davoine , Kristoffer Brännström , Nicolas Rouhier , Joakim Bygdell
Biochemical Journal, 2015, 468 (3), pp.385-400. ⟨10.1042/BJ20150132⟩
Journal articles hal-01269429v1

Function of glutathione peroxidases in legume root nodules

Manuel A. Matamoros , Ana Saiz , Maria Peñuelas , Pilar Bustos-Sanmamed , Jose M. Mulet
Journal of Experimental Botany, 2015, 66 (10), pp.2979-2990. ⟨10.1093/jxb/erv066⟩
Journal articles hal-01269091v1

The roles of glutaredoxins ligating Fe-S clusters: Sensing, transfer or repair functions?

Jérémy Couturier , Jonathan Przybyla-Toscano , Thomas Roret , Claude Didierjean , Nicolas Rouhier
Biochimica et Biophysica Acta - Molecular Cell Research, 2015, 1853 (6, SI), pp.1513-1527. ⟨10.1016/j.bbamcr.2014.09.018⟩
Journal articles hal-01521788v1
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Quinone- and nitroreductase reactions of [i]Thermotoga maritima[/i] thioredoxin reductase

Benjaminas Valiauga , Nicolas Rouhier , Jean-Pierre Jacquot
Acta Biochimica Polonica, 2015, 62 (2), pp.303-309. ⟨10.18388/abp.2015_1003⟩
Journal articles hal-01269392v1

The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana

Anna Moseler , Isabel Aller , Stephan Wagner , Thomas Nietzel , Jonathan Przybyla Toscano
Proceedings of the National Academy of Sciences of the United States of America, 2015, 112 (44), pp.13735-13740. ⟨10.1073/pnas.1510835112⟩
Journal articles hal-01269453v1

Involvement of thiol-based mechanisms in plant development

Nicolas Rouhier , Delphine Cerveau , Jérémy Couturier , Jean-Philippe Reichheld , Pascal Rey
Biochimica et Biophysica Acta (BBA) - General Subjects, 2015, 1850 (8, SI), pp.1479-1496. ⟨10.1016/j.bbagen.2015.01.023⟩
Journal articles hal-01579585v1
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Glutathionyl-hydroquinone reductases from poplar are plastidial proteins that deglutathionylate both reduced and oxidized glutathionylated quinones

Pierre-Alexandre Lallement , Edgar Meux , José M. Gualberto , Stéphane Dumarcay , Frédérique Favier
FEBS Letters, 2015, 589 (1), pp.37 - 44. ⟨10.1016/j.febslet.2014.11.021⟩
Journal articles hal-01522015v1

Arabidopsis glutaredoxin S17 and its partner NF-YC11/NC2α contribute to maintenance of the shoot apical meristem under long-day photoperiod

Johannes Knuesting , Christophe Riondet , Carlos Maria , Inga Kruse , Noëlle Becuwe
Plant Physiology, 2015, 167 (4), pp.1643-58. ⟨10.1104/pp.15.00049⟩
Journal articles hal-01140698v1

X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana

Thomas Roret , Henri Pégeot , Jérémy Couturier , Guillermo Mulliert , Nicolas Rouhier
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2014, 70 (9), pp.1180-1185. ⟨10.1107/S2053230X14017026⟩
Journal articles hal-01521313v1
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Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes

Thomas Roret , Pascale Tsan , Jérémy Couturier , Bo Zhang , Michael K. Johnson
Journal of Biological Chemistry, 2014, 289 (35), pp.24588-24598. ⟨10.1074/jbc.M114.572701⟩
Journal articles hal-01521194v1
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Characterization of poplar GrxS14 in different structural forms

Lei Wang , Yifei Li , Jean-Pierre Jacquot , Nicolas Rouhier , Bin Xia
Protein and Cell, 2014, 5 (5), pp.329-333. ⟨10.1007/s13238-014-0042-3⟩
Journal articles hal-01268571v1
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Genome analysis of poplar LRR-RLP gene clusters reveals RISP, a defense-related gene coding a candidate endogenous peptide elicitor

Benjamin Petre , Stéphane Hacquard , Sébastien Duplessis , Nicolas Rouhier
Frontiers in Plant Science, 2014, 5, ⟨10.3389/fpls.2014.00111⟩
Journal articles hal-02548502v1
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The still mysterious roles of cysteine-containing glutathione transferases in plants

Pierre-Alexandre Lallement , Bastiaan Brouwer , Olivier Keech , Arnaud Hecker , Nicolas Rouhier
Frontiers in Pharmacology, 2014, 5, 22 p. ⟨10.3389/fphar.2014.00192⟩
Journal articles hal-01268957v1
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The poplar Phi class glutathione transferase: expression, activity and structure of GSTF1

Henri Pégeot , Cha San Koh , Benjamin Petre , Sandrine Mathiot , Sébastien Duplessis
Frontiers in Plant Science, 2014, 5, article 712 (15 p.). ⟨10.3389/fpls.2014.00712⟩
Journal articles hal-01521321v1

Putative roles of glutaredoxin-BolA holo-heterodimers in plants

Tiphaine Dhalleine , Nicolas Rouhier , Jérémy Couturier
Plant Signaling and Behavior, 2014, 9 (5), pp. e28564. ⟨10.4161/psb.28564⟩
Journal articles hal-01579702v1

Monothiol Glutaredoxin-BolA Interactions: Redox Control of Arabidopsis thaliana BolA2 and SufE1

Jérémy Couturier , Hui-Chen Wu , Tiphaine Dhalleine , Henri Pégeot , Damien Sudre
Molecular Plant, 2014, 7 (1), pp.187-205. ⟨10.1093/mp/sst156⟩
Journal articles hal-00936792v1

Structural and enzymatic insights into Lambda glutathione transferases from Populus trichocarpa, monomeric enzymes constituting an early divergent class specific to terrestrial plants

Pierre-Alexandre Lallement , Edgar Meux , José Manuel Gualberto , Pascalita Prosper , Claude Didierjean
Biochemical Journal, 2014, 462 (1), pp.39-52. ⟨10.1042/BJ20140390⟩
Journal articles hal-01050560v1
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Thiol-based redox homeostasis and signaling

Francisco J Cejudo , Andreas A Meyer , Jean-Philippe J Reichheld , Nicolas Rouhier , Jose A Traverso
Frontiers in Plant Science, 2014, 5, pp.450 - 450. ⟨10.3389/fpls.2014.00266⟩
Journal articles hal-01579721v1
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Cysteine-based redox regulation and signaling in plants

Jérémy Couturier , Kamel Chibani , Jean-Pierre Jacquot , Nicolas Rouhier
Frontiers in Plant Science, 2013, 4, pp.105. ⟨10.3389/fpls.2013.00105⟩
Journal articles hal-01578659v1
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Mechanisms of Nitrosylation and Denitrosylation of Cytoplasmic Glyceraldehyde-3-phosphate Dehydrogenase from Arabidopsis thaliana

Mirko Zaffagnini , Samuel Morisse , Mariette Bedhomme , Christophe H. Marchand , Margherita Festa
Journal of Biological Chemistry, 2013, 288 (31), pp.22777 - 22789. ⟨10.1074/jbc.M113.475467⟩
Journal articles hal-01577781v1

In the Absence of Thioredoxins, What Are the Reductants for Peroxiredoxins in Thermotoga maritima?

Jérémy Couturier , Pascalita Prosper , Alison Winger-Mendes , Arnaud Hecker , Masakazu Hirasawa
Antioxidants and Redox Signaling, 2013, 18 (13), pp.1613 - 1622. ⟨10.1089/ars.2012.4739⟩
Journal articles hal-01268123v1

Monothiol glutaredoxins and A-type proteins: partners in Fe-S cluster trafficking

Daphne T. Mapolelo , Bo Zhang , Sajini Randeniya , Angela-Nadia Albetel , Haoran Li
Dalton Transactions, 2013, 42 (9), pp.3107 - 3115. ⟨10.1039/c2dt32263c⟩
Journal articles hal-01268219v1
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Toward a refined classification of class I dithiol glutaredoxins from poplar: biochemical basis for the definition of two subclasses

Jérémy Couturier , Jean-Pierre Jacquot , Nicolas Rouhier
Frontiers in Plant Science, 2013, 4, ⟨10.3389/fpls.2013.00518⟩
Journal articles hal-01268530v1
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Development of roGFP2-derived redox probes for measurement of the glutathione redox potential in the cytosol of severely glutathione-deficient rml1 seedlings

Isabel Aller , Nicolas Rouhier , Andreas J. Meyer
Frontiers in Plant Science, 2013, 4, ⟨10.3389/fpls.2013.00506⟩
Journal articles hal-01268531v1

Monothiol Glutaredoxins Can Bind Linear [Fe3S4](+) and [Fe4S4](2+) Clusters in Addition to [Fe2S2](2+) Clusters: Spectroscopic Characterization and Functional Implications

Bo Zhang , Sibali Bandyopadhyay , Priyanka Shakamuri , Sunil G. Naik , Boi Hanh Huynh
Journal of the American Chemical Society, 2013, 135 (40), pp.15153-15164. ⟨10.1021/ja407059n⟩
Journal articles hal-01578664v1

Arabidopsis thaliana Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for in Vitro Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins

Huanyao Gao , Sowmya Subramanian , Jérémy Couturier , Sunil G. Naik , Sung-Kun Kim
Biochemistry, 2013, 52 (38), pp.6633 - 6645. ⟨10.1021/bi4007622⟩
Journal articles hal-01268928v1

Overexpression, purification and enzymatic characterization of a recombinant plastidial glucose-6-phosphate dehydrogenase from barley (Hordeum vulgare cv. Nure) roots

Manuela Cardi , Kamel Chibani , Daniela Castiglia , Donata Cafasso , Elio Pizzo
Plant Physiology and Biochemistry, 2013, 73, pp.266-273. ⟨10.1016/j.plaphy.2013.10.008⟩
Journal articles hal-01268539v1

Two Sinorhizobium meliloti glutaredoxins regulate iron metabolism and symbiotic bacteroid differentiation

Sofiane M. Benyamina , Fabien Baldacci-Cresp , Jérémy Couturier , Kamel Chibani , Julie Hopkins
Environmental Microbiology, 2013, 15 (3), pp.795 - 810. ⟨10.1111/j.1462-2920.2012.02835.x⟩
Journal articles hal-01577790v1
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The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions.

Jérémy Couturier , Brigitte Touraine , Jean-François Briat , Frédéric Gaymard , Nicolas Rouhier
Frontiers in Plant Science, 2013, 4, pp.259. ⟨10.3389/fpls.2013.00259⟩
Journal articles hal-00920681v1
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RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter

Benjamin Petre , Emmanuelle Morin , Emilie Tisserant , Stéphane Hacquard , Corinne da Silva
PLoS ONE, 2012, 7 (8), pp.1-12. ⟨10.1371/journal.pone.0044408⟩
Journal articles hal-01267887v1

Glutathione- and glutaredoxin-dependent reduction of methionine sulfoxide reductase A

Jérémy Couturier , Florence Vignols , Jean-Pierre Jacquot , Nicolas Rouhier
FEBS Letters, 2012, 586 (21), pp.3894-3899. ⟨10.1016/j.febslet.2012.09.020⟩
Journal articles hal-02643185v1

Glutathione regulates the transfer of iron-sulfur cluster from monothiol and dithiol glutaredoxins to apo ferredoxin

Lei Wang , Bingjie Ouyang , Yifei Li , Yingang Feng , Jean-Pierre Jacquot
Protein and Cell, 2012, 3 (9), pp.714-721. ⟨10.1007/s13238-012-2051-4⟩
Journal articles hal-03386454v1

Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro

Mariette Bedhomme , Mattia Adamo , Christophe H. Marchand , Jérémy Couturier , Nicolas Rouhier
Biochemical Journal, 2012, 445, pp.337 - 347. ⟨10.1042/BJ20120505⟩
Journal articles hal-01268311v1

Affinity chromatography: a valuable strategy to isolate substrates of methionine sulfoxide reductases?

Lionel Tarrago , Sylvie Kieffer-Jaquinod , Tiphaine Lamant Dhalleine , Marlene Marcellin , Jerome Garin
Antioxidants and Redox Signaling, 2012, 16 (1), pp.79 - 84. ⟨10.1089/ars.2011.4153⟩
Journal articles hal-01268395v1
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Atypical thioredoxins in poplar: the glutathione-dependent thioredoxin-like 2.1 supports the activity of target enzymes possessing a single redox active cysteine

Kamel Chibani , Lionel Tarrago , José Manuel Gualberto , Gunnar Wingsle , Pascal Rey
Plant Physiology, 2012, 159 (2), pp.592-605. ⟨10.1104/pp.112.197723⟩
Journal articles hal-01137170v1

Glutaredoxin S12: Unique Properties for Redox Signaling

Mirko Zaffagnini , Mariette Bedhomme , Christophe H. Marchand , Jérémy Couturier , Xing-Huang Gao
Antioxidants and Redox Signaling, 2012, 16 (1), pp.17 - 32. ⟨10.1089/ars.2011.3933⟩
Journal articles hal-01268394v1
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Quinone- and nitroreductase reactions of Thermotoga maritima 3 peroxiredoxin–nitroreductase hybrid enzyme

Zilvinas Anusevicius , Lina Misevičienė , Nicolas Rouhier , Jean-Pierre Jacquot
Archives of Biochemistry and Biophysics, 2012, 528 (1), pp.50-56. ⟨10.1016/j.abb.2012.08.014⟩
Journal articles hal-01332162v1

Hydroperoxide and peroxynitrite reductase activity of poplar thioredoxin-dependent glutathione peroxidase 5: kinetics, catalytic mechanism and oxidative inactivation.

Benjamin Selles , Martin Hugo , Madia Trujillo , Vaibhav Srivastava , Gunnar Wingsle
Biochemical Journal, 2012, 442 (2), pp.369-380. ⟨10.1042/BJ20111378⟩
Journal articles hal-01268025v1
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The poplar-poplar rust interaction: insights from genomics and transcriptomics

Stéphane S. Hacquard , Benjamin Petre , Pascal Frey , Arnaud Hecker , Nicolas Rouhier
Journal of Pathogens, 2011, EPUB 2011
Journal articles hal-02642064v1

Biochemical properties of poplar thioredoxin z.

Kamel Chibani , Lionel Tarrago , Peter Schürmann , Jean-Pierre Jacquot , Nicolas Rouhier
FEBS Letters, 2011, 585 (7), pp.1077-1081. ⟨10.1016/j.febslet.2011.03.006⟩
Journal articles hal-02644309v1

Cadmium Affects the Glutathione/Glutaredoxin System in Germinating Pea Seeds

Moez Smiri , Abdelilah Chaoui , Nicolas Rouhier , Éric Gelhaye , Jean-Pierre Jacquot
Biological Trace Element Research, 2011, 142 (1), pp.93 - 105. ⟨10.1007/s12011-010-8749-3⟩
Journal articles hal-02652310v1
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Arabidopsis Chloroplastic Glutaredoxin C5 as a Model to Explore Molecular Determinants for Iron-Sulfur Cluster Binding into Glutaredoxins

Jérémy Couturier , Elke Ströher , Angela-Nadia Albetel , Thomas Roret , Meenakumari Muthuramalingam
Journal of Biological Chemistry, 2011, 286 (31), pp.27515-27527. ⟨10.1074/jbc.M111.228726⟩
Journal articles hal-02545674v1

H-1, C-13, and N-15 resonance assignments of reduced GrxS14 from Populus tremula x tremuloides

Lei Wang , Xiaobai Ren , Yifei Li , Nicolas Rouhier , Jean-Pierre Jacquot
Biomolecular NMR Assignments, 2011, 5 (1), pp.121 - 124. ⟨10.1007/s12104-010-9282-1⟩
Journal articles hal-02642042v1
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Genome-wide analysis of eukaryote thaumatin-like proteins (TLPs) with an emphasis on poplar

Benjamin Petre , Ian Major , Nicolas Rouhier , Sébastien Duplessis
BMC Plant Biology, 2011, 11 (1), pp.33. ⟨10.1186/1471-2229-11-33⟩
Journal articles hal-02548481v1

Comparative genomic study of protein disulfide isomerases from photosynthetic organisms

Benjamin Selles , Jean-Pierre Jacquot , Nicolas Rouhier
Genomics, 2011, 97 (1), pp.37 - 50. ⟨10.1016/j.ygeno.2010.10.001⟩
Journal articles hal-02648687v1

Two-electron reduction of nitroaromatic compounds by Thermotoga maritima hybrid peroxiredoxin-nitroreductase enzyme

Nicolas Rouhier , Jean-Pierre Jacquot , Lina Miseviciene , Zilvinas Anusevicius , Jonas Sarlauskas
Chemija, 2011, 22 (2), pp.143 - 148
Journal articles hal-02649470v1

Characterization and manipulation of the pathway-specific late regulator AlpW reveals Streptomyces ambofaciens as a new producer of kinamycins

R. Bunet , L. Song , M. Vaz Mendez , C. Corre , L. Hotel
Journal of Bacteriology, 2011, 193 ((5)), pp. 1142-1153. ⟨10.1128/JB.01269-10⟩
Journal articles hal-00606766v1

Peroxiredoxins and NADPH-Dependent Thioredoxin Systems in the Model Legume Lotus japonicus

Alejandro Tovar-Méndez , Manuel Matamoros , Pilar Bustos-Sanmamed , Karl-Josef Dietz , Francisco Javier Cejudo
Plant Physiology, 2011, 156 (3), pp.1535-1547. ⟨10.1104/pp.111.177196⟩
Journal articles hal-03386456v1
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Obligate biotrophy features unraveled by the genomic analysis of rust fungi

Sébastien Duplessis , Christina A C. A. Cuomo , Yao-Cheng Y.-C. Lin , Andrea Aerts , Emilie Tisserant
Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (22), pp.9166-9171. ⟨10.1073/pnas.1019315108⟩
Journal articles hal-01000169v1

Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenase

Manuela Cardi , Kamel Chibani , Donata Cafasso , Nicolas Rouhier , Jean-Pierre Jacquot
Journal of Experimental Botany, 2011, 62 (11), pp.4013 - 4023. ⟨10.1093/jxb/err100⟩
Journal articles hal-02649210v1

Introduction

Jean-Pierre Jacquot , Nicolas Rouhier
Photosynthesis Research, 2010, 104 (1), pp.1-3. ⟨10.1007/s11120-010-9550-z⟩
Journal articles hal-02657238v1

Plant glutaredoxins

Nicolas Rouhier
New Phytologist, 2010, 186 (2), pp.365-372. ⟨10.1111/j.1469-8137.2009.03146.x⟩
Journal articles hal-02663806v1

Oxidative damage and redox change in pea seeds treated with cadmium

Moez Smiri , Abdelilah Chaoui , Nicolas Rouhier , Éric Gelhaye , Jean-Pierre Jacquot
Comptes Rendus Biologies, 2010, 333 (11-12), pp.801 - 807. ⟨10.1016/j.crvi.2010.09.002⟩
Journal articles hal-02667925v1

The chloroplastic thiol reducing systems

Kamel Chibani , Jérémy Couturier , Benjamin Selles , Jean-Pierre Jacquot , Nicolas Rouhier
Photosynthesis Research, 2010, 104 (1), pp.75-99. ⟨10.1007/s11120-009-9501-8⟩
Journal articles hal-02667673v1

Engineered mutated glutaredoxins mimicking peculiar plant class III glutaredoxins bind iron-sulfur centers and possess reductase activity

Jérémy Couturier , Claude Didierjean , Jean-Pierre Jacquot , Nicolas Rouhier
Biochemical and Biophysical Research Communications, 2010, 403 (3-4), pp.435 - 441. ⟨10.1016/j.bbrc.2010.11.050⟩
Journal articles hal-02662291v1

Glutaredoxins: roles in iron homeostasis

Nicolas Rouhier , Jérémy Couturier , Michael Johnson , Jean-Pierre Jacquot
Trends in Biochemical Sciences, 2010, 35 (1), pp.43-52. ⟨10.1016/j.tibs.2009.08.005⟩
Journal articles hal-02545637v1

Cadmium induced mitochondrial redox changes in germinating pea seed

Moez Smiri , Abdelilah Chaoui , Nicolas Rouhier , Kamel Chibani , Éric Gelhaye
BioMetals, 2010, 23 (6), pp.973-984. ⟨10.1007/s10534-010-9344-y⟩
Journal articles hal-02663288v1
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Structure-Function Relationship of the Chloroplastic Glutaredoxin S12 with an Atypical WCSYS Active Site

Jérémy Couturier , Cha San Koh , Mirko Zaffagnini , Alison Winger , José Manuel Gualberto
Journal of Biological Chemistry, 2009, 284 (14), pp.9299-9310. ⟨10.1074/jbc.M807998200⟩
Journal articles hal-02545616v1

Alternative splicing studies of the reactive oxygen species gene network in populus reveal two isoforms of high-isoelectric-point superoxide dismutase

Vaibhav Srivastava , Manoj Kumar Srivastava , Kamel Chibani , Robert Nilsson , Nicolas Rouhier
Plant Physiology, 2009, 149 (4), pp.1848-1859. ⟨10.1104/pp.108.133371⟩
Journal articles hal-02662132v1
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Regeneration mechanisms of arabidopsis thaliana methionine sulfoxide reductases B by glutaredoxins and thioredoxins

Lionel Tarrago , Edith Laugier , Mirko Zaffagnini , Christophe Marchand , Pierre Le Maréchal
Journal of Biological Chemistry, 2009, 284 (28), pp.18963-18971. ⟨10.1074/jbc.M109.015487⟩
Journal articles hal-02665795v1

Evolution and diversity of glutaredoxins in photosynthetic organisms

Jérémy Couturier , Jean-Pierre Jacquot , Nicolas Rouhier
Cellular and Molecular Life Sciences, 2009, 66 (15), pp.2539-2557. ⟨10.1007/s00018-009-0054-y⟩
Journal articles hal-02668190v1

Structural and evolutionary aspects of thioredoxin reductases in photosynthetic organisms

Jean-Pierre Jacquot , Hans Eklund , Nicolas Rouhier , Peter Schürmann
Trends in Plant Science, 2009, 14 (6), pp.336-343. ⟨10.1016/j.tplants.2009.03.005⟩
Journal articles hal-02656851v1

Comparative genomic study of the thioredoxin family in photosynthetic organisms with emphasis on populus trichocarpa

Kamel Chibani , Gunnar Wingsle , Jean-Pierre Jacquot , Éric Gelhaye , Nicolas Rouhier
Molecular Plant, 2009, 2 (2), pp.308-322. ⟨10.1093/mp/ssn076⟩
Journal articles hal-02667708v1

The glutathione peroxidase gene family of Lotus japonicus

Javier Ramos , Manuel A. Matamoros , Loreto Naya , Euan K. James , Nicolas Rouhier
New Phytologist, 2009, 181 (1), pp.103-114. ⟨10.1111/j.1469-8137.2008.02629.x⟩
Journal articles hal-02667256v1
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An atypical catalytic mechanism involving three cysteines of thioredoxin

Cha San Koh , Nicolas Navrot , Claude Didierjean , Nicolas Rouhier , Masakazu Hirasawa
Journal of Biological Chemistry, 2008, 283 (34), pp.23062-23072. ⟨10.1074/jbc.M802093200⟩
Journal articles hal-02667186v1

Functional analysis and expression characteristics of chloroplastic Prx IIE

Filipe Gama , Claire Bréhélin , Éric Gelhaye , Yves Meyer , Jean-Pierre Jacquot
Physiologia Plantarum, 2008, 133 (3), pp.599-610. ⟨10.1111/j.1399-3054.2008.01097.x⟩
Journal articles hal-02118623v1

Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe–2S] clusters

Sibali Bandyopadhyay , Filipe Gama , Maria Micaela Molina‐navarro , José Manuel Gualberto , Ronald Claxton
EMBO Journal, 2008, 27 (7), pp.1122-1133. ⟨10.1038/emboj.2008.50⟩
Journal articles hal-01332174v1
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The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis

F. Martin , A. Aerts , D. Ahrén , A. Brun , E. G. J. Danchin
Nature, 2008, 452 (7183), pp.88-93. ⟨10.1038/nature06556⟩
Journal articles halsde-00261893v1
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Regulation of the Synthesis of the Angucyclinone Antibiotic Alpomycin in Streptomyces ambofaciens by the Autoregulator Receptor AlpZ and Its Specific Ligand

Robert Bunet , M. V. V Mendes , Nicolas Rouhier , Xiuhua Pang , L. Hotel
Journal of Bacteriology, 2008, 190 (9), pp.3293 - 3305. ⟨10.1128/JB.01989-07⟩
Journal articles hal-01659448v1

Getting sick may help plants overcome abiotic stress

Nicolas Rouhier , Jean-Pierre Jacquot
New Phytologist, 2008, 180 (4), pp.738-741. ⟨10.1111/j.1469-8137.2008.02673.x⟩
Journal articles hal-02659237v1
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The Role of Glutathione in Photosynthetic Organisms: Emerging Functions for Glutaredoxins and Glutathionylation

Nicolas Rouhier , Stéphane D. Lemaire , Jean-Pierre Jacquot
Annual Review of Plant Biology, 2008, 59, pp.143-166. ⟨10.1146/annurev.arplant.59.032607.092811⟩
Journal articles hal-02660326v1

Comparison of the thiol-dependent antioxidant systems in the ectomycorrhizal Laccaria bicolor and the saprotrophic Phanerochaete chrysosporium

Mélanie Morel , Annegret Kohler , Francis Martin , Éric Gelhaye , Nicolas Rouhier
New Phytologist, 2008, 180 (2), pp.391-407. ⟨10.1111/j.1469-8137.2008.02498.x⟩
Journal articles hal-02655538v1
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Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima

Carole Barbey , Nicolas Rouhier , Ahmed Haouz , Alda Navaza , Jean-Pierre Jacquot
Acta crystallographica Section F : Structural biology communications [2014-..], 2008, 64 (1), pp.29-31. ⟨10.1107/S1744309107064391⟩
Journal articles hal-01332178v1

Specificity of thioredoxins and glutaredoxins as electron donors to two distinct classes of Arabidopsis plastidial methionine sulfoxide reductases B

Christina Vieira dos Santos , Edith Laugier , Lionel Tarrago , Vincent Massot , Emmanuelle Issakidis-Bourguet
FEBS Letters, 2007, 581 (23), pp.4371-4376. ⟨10.1016/j.febslet.2007.07.081⟩
Journal articles hal-02929740v1

Crystal Structures of a Poplar Thioredoxin Peroxidase that Exhibits the Structure of Glutathione Peroxidases: Insights into Redox-driven Conformational Changes

Cha San Koh , Claude Didierjean , Nicolas Navrot , Santosh Panjikar , Guillermo Mulliert
Journal of Molecular Biology, 2007, 370 (3), pp.512-529. ⟨10.1016/j.jmb.2007.04.031⟩
Journal articles hal-03386486v1

PeroxiBase: The peroxidase database

Filippo Passardi , Grégory Theiler , Marcel Zamocky , Claudia Cosio , Nicolas Rouhier
Phytochemistry, 2007, 68 (12), pp.1605-1611. ⟨10.1016/j.phytochem.2007.04.005⟩
Journal articles hal-01999868v1

Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer

Andreas Meyer , Thorsten Brach , Laurent Marty , Susanne Kreye , Nicolas Rouhier
Plant Journal, 2007, 52 (5), pp.973-986. ⟨10.1111/j.1365-313X.2007.03280.x⟩
Journal articles hal-03386481v1

Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin

N. Rouhier , H. Unno , S. Bandyopadhyay , L. Masip , S.K. Kim
Proceedings of the National Academy of Sciences of the United States of America, 2007, 104, pp.7379-7384
Journal articles hal-00253984v1

Redox based anti-oxidant systems in plants: Biochemical and structural analyses

Nicolas Rouhier , Cha San Koh , Éric Gelhaye , Catherine Corbier , Frederique Favier
Biochimica et Biophysica Acta (BBA) - General Subjects, 2007, 1780 (11), pp.1249-1260. ⟨10.1016/j.bbagen.2007.12.007⟩
Journal articles hal-02662632v1
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Functional and structural aspects of poplar cytosolic and plastidial type A methionine sulfoxide reductases

Nicolas Rouhier , Brice Kauffmann , Frédérique Tete-Favier , Pasquale Palladino , Pierre Gans
Journal of Biological Chemistry, 2007, 282 (5), pp.3367-3378. ⟨10.1074/jbc.M605007200⟩
Journal articles hal-02655515v1

The mitochondrial type II peroxiredoxin from poplar

Filipe Gama , Olivier Keech , Françoise Eymery , Iris Finkemeier , Éric Gelhaye
Physiologia Plantarum, 2007, 129 (1), pp.196-206. ⟨10.1111/j.1399-3054.2006.00785.x⟩
Journal articles hal-02667238v1

Ascorbate peroxidase–thioredoxin interaction

Éric Gelhaye , Nicolas Navrot , Isabel K. Macdonald , Nicolas Rouhier , Emma Lloyd Raven
Photosynthesis Research, 2006, 89 (2-3), pp.193-200. ⟨10.1007/s11120-006-9100-x⟩
Journal articles hal-02655714v1

Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses

Nicolas Navrot , Valérie Collin , José Gualberto , Éric Gelhaye , Masakazu Hirasawa
Plant Physiology, 2006, 142 (4), pp.1364-1379. ⟨10.1104/pp.106.089458⟩
Journal articles hal-02669180v1

Plant methionine sulfoxide reductase A and B multigenic families

Nicolas Rouhier , Christina Vieira Dos Santos , Lionel Tarrago , Pascal Rey
Photosynthesis Research, 2006, 89 (2-3), pp.247-262. ⟨10.1007/s11120-006-9097-1⟩
Journal articles hal-02929742v1

Engineering functional artificial hybrid proteins between poplar peroxiredoxin II and glutaredoxin or thioredoxin

Nicolas Rouhier , Filipe Gama , Gunnar Wingsle , Éric Gelhaye , Pierre Gans
Biochemical and Biophysical Research Communications, 2006, 341 (4), pp.1300-1308. ⟨10.1016/j.bbrc.2006.01.099⟩
Journal articles hal-03141541v1

Engineering functional artificial hybrid proteins between poplar peroxiredoxin II and glutaredoxin or thioredoxin

Nicolas Rouhier , Filipe Gama , Gunnar Wingsle , Pierre Gans , Jean-Pierre Jacquot
Biochemical and Biophysical Research Communications, 2006, 341 (4), pp.1300-1308. ⟨10.1016/j.bbrc.2006.01.099⟩
Journal articles hal-02653205v1
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Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimer

Valérie Noguera-Mazon , Jérôme Lemoine , Olivier Walker , Nicolas Rouhier , Arnaud Salvador
Journal of Biological Chemistry, 2006, 381 (42), pp.31736-31742. ⟨10.1074/jbc.M602188200⟩
Journal articles hal-02661917v1

Identification of a new family of plant proteins loosely related to glutaredoxins with four CxxC motives

Nicolas Navrot , Éric Gelhaye , Jean-Pierre Jacquot , Nicolas Rouhier
Photosynthesis Research, 2006, 89 (2-3), pp.71-79. ⟨10.1007/s11120-006-9083-7⟩
Journal articles hal-02665437v1

Structural insight into poplar glutaredoxin C1 with a bridging iron-sulfur cluster at the active site

Yingang Feng , Nan Zhong , Nicolas Rouhier , Toshiharu Hase , Masami Kusunoki
Biochemistry, 2006, 45 (26), pp.7998-8008. ⟨10.1021/bi060444t⟩
Journal articles hal-02655662v1

Genome-wide analysis of plant glutaredoxin systems

Nicolas Rouhier , Jérémy Couturier , Jean-Pierre Jacquot
Journal of Experimental Botany, 2006, 57 (8), pp.1685-1696. ⟨10.1093/jxb/erl001⟩
Journal articles hal-02545530v1

NMR reveals a novel glutaredoxin-glutaredoxin interaction interface

V. Noguera , O. Walker , Nicolas Rouhier , J.P. Jacquot , I. Krimm
Journal of Molecular Biology, 2005, 353 (3), pp.629-641. ⟨10.1016/j.jmb.2005.08.035⟩
Journal articles hal-02679450v1

Identification of Plant Glutaredoxin Targets

Nicolas Rouhier , Arsenio Villarejo , Manoj K Srivastava , Éric Gelhaye , Olivier Keech
Antioxidants and Redox Signaling, 2005, 7 (7-8), pp.919-929. ⟨10.1089/ars.2005.7.919⟩
Journal articles hal-02321218v1

Analysis of the proteins targeted by CDSP32, a plastidic thioredoxin participating in oxidative stress responses

P. Rey , S. Cuine , F. Eymery , G. Garin , M. Court
Plant Journal, 2005, 41, pp.31-42
Journal articles hal-02676534v1

The Arabidopsis Plastidic Methionine Sulfoxide Reductase B Proteins. Sequence and Activity Characteristics, Comparison of the Expression with Plastidic Methionine Sulfoxide Reductase A, and Induction by Photooxidative Stress

Christina Vieira dos Santos , Stéphan Cuiné , Nicolas Rouhier , Pascal Jean Del Rey
Plant Physiology, 2005, 138 (2), pp.909-922. ⟨10.1104/pp.105.062430⟩
Journal articles hal-03187532v1

Crystal Structure and Solution NMR Dynamics of a D (Type II) Peroxiredoxin Glutaredoxin and Thioredoxin Dependent: A New Insight into the Peroxiredoxin Oligomerism

Aude Echalier , Xavier Trivelli , Catherine Corbier , Nicolas Rouhier , Olivier Walker
Biochemistry, 2005, 44 (6), pp.1755-1767. ⟨10.1021/bi048226s⟩
Journal articles hal-02680941v1

Synergistic wood preservatives involving EDTA, irganox 1076 and 2-hydroxypyridine-N-oxide

A. Mabicka , Stéphane Dumarçay , Nicolas Rouhier , Michel Linder , J.P. Jacquot
International Biodeterioration and Biodegradation, 2005, 55, pp.203-211
Journal articles hal-02680142v1

The plant multigenic family of thiol peroxidases

Nicolas Rouhier , J.P. Jacquot
Free Radical Biology and Medicine, 2005, 38, pp.1413-1421
Journal articles hal-02679702v1

The plant thioredoxin system

Éric Gelhaye , Nicolas Rouhier , Nicolas Navrot , Jean-Pierre Jacquot
Cellular and Molecular Life Sciences, 2005, 62 (1), pp.24-35. ⟨10.1007/s00018-004-4296-4⟩
Journal articles hal-02679065v1

Letter to the Editor: 1H, 15N, and 13C resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides

Yingang Feng , Nicolas Rouhier , Jean-Pierre Jacquot , Bin Xia
Journal of Biomolecular NMR, 2005, 31 (3), pp.263-264. ⟨10.1007/s10858-005-0497-x⟩
Journal articles hal-02677439v1

Analysis of the proteins targeted by CDSP32, a plastidic thioredoxin participating in oxidative stress responses

Pascal Jean Del Rey , Stéphan Cuiné , Françoise Eymery , Jérome Garin , Magali Court
Plant Journal, 2005, 41 (1), pp.31-42. ⟨10.1111/j.1365-313X.2004.02271.x⟩
Journal articles hal-03187481v1

A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase

Éric Gelhaye , Nicolas Rouhier , J. Gerard , Yves Jolivet , José Gualberto
Proceedings of the National Academy of Sciences of the United States of America, 2004, 101 (40), pp.14545-14550
Journal articles hal-02680989v1

The thioredoxin h system of higher plants

Éric Gelhaye , Nicolas Rouhier , J.P. Jacquot
Plant Physiology and Biochemistry, 2004, 42, pp.265-271
Journal articles hal-02673403v1
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Poplar peroxiredoxin Q. A thioredoxin-linked chloroplast antioxidant functional in pathogen defense

Nicolas Rouhier , Éric Gelhaye , J.M. Gualberto , M.N. Jordy , E. de Fay
Plant Physiology, 2004, 134 (3), pp.1027-1036
Journal articles hal-02680157v1

Active site mutagenesis and phospholipid hydroperoxide reductase activity of poplar type II peroxiredoxin

Nicolas Rouhier , Éric Gelhaye , C. Corbier , J.P. Jacquot
Physiologia Plantarum, 2004, 120, pp.57-62
Journal articles hal-02672433v1

Letter to the Editor: 1H, 13C and 15N NMR assignment of the homodimeric poplar phloem type II peroxiredoxin

Sandrine Bouillac , Nicolas Rouhier , Pascale Tsan , Jean-Pierre Jacquot , Jean-Marc Lancelin
Journal of Biomolecular NMR, 2004, 30 (1), pp.105-106. ⟨10.1023/B:JNMR.0000042965.46172.1b⟩
Journal articles hal-02677501v1

Letter to the Editor: 1H, 13C and 15N resonance assignments of poplar phloem glutaredoxin

Valérie Noguera , Nicolas Rouhier , Isabelle Krimm , Jean-Pierre Jacquot , Jean-Marc Lancelin
Journal of Biomolecular NMR, 2004, 30 (2), pp.219-220. ⟨10.1023/B:JNMR.0000048849.02529.66⟩
Journal articles hal-02681554v1

Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria

Yves Balmer , William H. Vensel , Charlene K. Tanaka , William J. Hurkman , Éric Gelhaye
Proceedings of the National Academy of Sciences of the United States of America, 2004, 101 (8), pp.2642-2647. ⟨10.1073/pnas.0308583101⟩
Journal articles hal-01332173v1

Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research

Jean-Pierre Jacquot , Nicolas Rouhier , Nicolas Navrot , Jean-Pierre Decottignies
Biotechnology and Applied Biochemistry, 2004, 40 (2), pp.181-185. ⟨10.1042/BA20030196⟩
Journal articles hal-02671937v1

Plant glutaredoxins: still mysterious reducing systems

N. Rouhier , Éric Gelhaye , J.-P. Jacquot
Cellular and Molecular Life Sciences, 2004, 61 (11), pp.1266-1277. ⟨10.1007/s00018-004-3410-y⟩
Journal articles hal-03143834v1

Molecular and catalytic properties of a peroxiredoxin-glutaredoxin hybrid from Neisseria meningitidis

Nicolas Rouhier , Jean-Pierre Jacquot
FEBS Letters, 2003, 554 (1-2), pp.149-153. ⟨10.1016/s0014-5793(03)01156-6⟩
Journal articles hal-03386458v1

Characterization of the Redox Properties of Poplar Glutaredoxin

Nicolas Rouhier , Alexios Vlamis-Gardikas , Christopher Horst Lillig , Carsten Berndt , Jens-Dirk Schwenn
Antioxidants and Redox Signaling, 2003, 5 (1), pp.15-22. ⟨10.1089/152308603321223504⟩
Journal articles hal-03386460v1

Evidence for a subgroup of thioredoxin h that requires GSH/Grx for its reduction

Éric Gelhaye , Nicolas Rouhier , J.P. Jacquot
FEBS Letters, 2003, 555, pp.443-448
Journal articles hal-02679184v1

Crystallization and preliminary X-ray studies of the glutaredoxin from poplar in complex with glutathione

K. d'Ambrosio , B. Kauffman , Nicolas Rouhier , E. Benedetti , J.P. Jacquot
Acta crystallographica Section D : Structural biology [1993-..], 2003, D59, pp.1043-1045. ⟨10.1107/S0907444903006814⟩
Journal articles hal-02674741v1

Identification and characterization of a third thioredoxin h in poplar

Éric Gelhaye , Nicolas Rouhier , A. Vlamis-Gardikas , J.M. Girardet , P.E. Sautière
Plant Physiology and Biochemistry, 2003, 41, pp.629-635
Journal articles hal-02675821v1

Enhancement of Poplar Glutaredoxin Expression by Optimization of the cDNA Sequence

Nicolas Rouhier , Éric Gelhaye , Pierre-Eric Sautière , Jean-Pierre Jacquot
Protein Expression and Purification, 2002, 24 (2), pp.234-241. ⟨10.1006/prep.2001.1574⟩
Journal articles hal-03141520v1

Isolation and characterization of an extended thioredoxin h from poplar

Éric Gelhaye , Nicolas Rouhier , Pascal Laurent , Pierre-Eric Sautière , Francis Martin
Physiologia Plantarum, 2002, 114 (2), pp.165-171. ⟨10.1034/j.1399-3054.2002.1140202.x⟩
Journal articles hal-03141495v1

Glutaredoxin-dependent Peroxiredoxin from Poplar

Nicolas Rouhier , Éric Gelhaye , Jean-Pierre Jacquot
Journal of Biological Chemistry, 2002, 277 (16), pp.13609-13614. ⟨10.1074/jbc.M111489200⟩
Journal articles hal-03141509v1

Redox Control by Dithiol-Disulfide Exchange in Plants

Nicolas Rouhier , Éric Gelhaye , Jean-Pierre Jacquot
Annals of the New York Academy of Sciences, 2002, 973 (1), pp.520-528. ⟨10.1111/j.1749-6632.2002.tb04693.x⟩
Journal articles hal-03141516v1

Crytallization and preliminary X-ray data of a bifunctional peroxiredoxin from poplar

A. Echalier , C. Corbier , Nicolas Rouhier , J.P. Jacquot , A. Aubry
Acta crystallographica Section D : Structural biology [1993-..], 2002, D58, pp.1501-1503. ⟨10.1107/S0907444902011782⟩
Journal articles hal-02673347v1

Redox Control by Dithiol-Disulfide Exchange in Plants

Jean-Pierre Jacquot , Nicolas Rouhier , Éric Gelhaye
Annals of the New York Academy of Sciences, 2002, 973 (1), pp.508-519. ⟨10.1111/j.1749-6632.2002.tb04692.x⟩
Journal articles hal-03141505v1

Plant peroxiredoxins: alternative hydroperoxide scavenging enzymes

Nicolas Rouhier , Jean-Pierre Jacquot
Photosynthesis Research, 2002, 74 (3), pp.259-268. ⟨10.1023/A:1021218932260⟩
Journal articles hal-03386459v1

Thioredoxins and related proteins in photosynthetic organisms: molecular basis for thiol dependent regulation

Jean-Pierre Jacquot , Éric Gelhaye , Nicolas Rouhier , Catherine Corbier , Claude Didierjean
Biochemical Pharmacology, 2002, 64 (5-6), pp.1065-1069. ⟨10.1016/S0006-2952(02)01177-2⟩
Journal articles hal-03141500v1

Exploring the active site of plant glutaredoxin by site-directed mutagenesis

Nicolas Rouhier , Éric Gelhaye , Jean-Pierre Jacquot
FEBS Letters, 2002, 511 (1-3), pp.145-149. ⟨10.1016/S0014-5793(01)03302-6⟩
Journal articles hal-03141514v1

Isolation and characterization of a new peroxiredoxin from poplar sieve tubes that uses either glutaredoxin or thioredoxin as a proton donor

Nicolas Rouhier , Éric Gelhaye , Pierre-Eric Sautiere , Annick Brun , Pascal Laurent
Plant Physiology, 2001, 127 (3), pp.1299-1309. ⟨10.1104/pp.010586⟩
Journal articles hal-02915286v1

Role of the chloroplastic iron-sulfur transfer protein NFU1 in the microalga Chlamydomonas reinhardtii

Jonathan Przybyla-Toscano , Nicolas Rouhier , Claire Remacle
Brussels Institute for Advanced Studies - Photosynthesis and primary production, Christian Hermans, Feb 2022, e-conference, Belgium
Conference papers hal-04176818v1

Gene atlas of iron-containing proteins (ironOME) in Arabidopsis thaliana

Jonathan Przybyla-Toscano , Clément Boussardon , Simon R. Law , Nicolas Rouhier , Olivier Keech
Iron Nutrition and Interactions in Plants, Jul 2022, Reims, France
Conference papers hal-04176793v1

Deciphering the biochemical relationships between cysteine desulfurase and sulfurtransferase protein families in sulfur trafficking

Damien Caubrière , Benjamin Selles , Anna Moseler , Sheng-Kai Sun , Morgane Ziesel
12th International Plant Sulfur Workshop, Jul 2022, London, Canada
Conference papers hal-03775257v1

Redox properties of the plant atypical thioredoxin DCC1.

Natacha Donnay , Tiphaine Dhalleine , Flavien Zannini , Nicolas Rouhier , Linda de Bont
Redox Biology Congress, Aug 2022, Ghent, Belgium
Conference papers hal-03781876v1

The chloroplastic iron-sulfur transfer protein NFU1 is required for chlorophyll synthesis in the dark and fermentation in the green microalga Chlamydomonas reinhardtii

Nicolas Rouhier , Jonathan Przybyla-Toscano , Claire Remacle
Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Sep 2022, Sainte Maxime, France
Conference papers hal-03781853v1

3-mercaptopyruvate sulfurtransferases: new actors in sulfur trafficking and H2S mediated-redox signalling in plants?

Anna Moseler , Tiphaine Dhalleine , Nicolas Rouhier , Jérémy Couturier
Redox Biology Congress 2022 Oxidative stress, redox biology and antioxidants from plants to humans, Aug 2022, Ghent, Belgium
Conference papers hal-03775274v1

Maturation of iron-sulfur proteins in chloroplasts: focus on the NFU transfer proteins

Nicolas Rouhier
SPS “Symposium on redox regulation and carbon-nitrogen interactions in plants. A tribute to Pierre Gadal”, Jun 2022, Gif sur Yvette, France
Conference papers hal-03781860v1

Rhodanese domain-containing sulfurtransferase family: a yet mysterious component of sulfur trafficking in plants

Anna Moseler , Benjamin Selles , Damien Caubrière , Sheng-Kai Sun , Morgane Ziesel
12th International Plant Sulfur Workshop, Jul 2022, London, Canada
Conference papers hal-03775204v1

The critical role of glutaredoxin S15 for the maturation of iron-sulfur proteins in mitochondria.

Loïck Christ , Anna Moseler , Jérémy Couturier , Pedroletti Luca , Andreas J. Meyer
12th International Conference for Plant Mitochondrial Biology 2022, May 2022, Malmö, Sweden
Conference papers hal-03705989v1

Towards the identification of novel metal- or thiol-based regulated proteins in plants

Tiphaine Dhalleine , Mathieu Schwartz , Flavien Zannini , Linda de Bont , Jérémy Couturier
International Symposium of the DFG Priority Program 1710 Dynamics of Thiol-based Redox Switches in Cellular Physiology, Mar 2021, e-conference, Germany
Conference papers hal-03197988v1

Role of the chloroplastic NFU3 iron-sulfur transfer protein in the microalga Chlamydomonas reinhardtii.

Claire Remacle , Véronique Larosa , Jonathan Przybyla-Toscano , Nicolas Rouhier
International Bioenergy and environment congress, Feb 2021, e-conference, France
Conference papers hal-03197977v1

The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in plant mitochondria.

Jonathan Przybyla-Toscano , Andrew Mclean , Olivier Keech , Janneke Balk , Nicolas Rouhier
Congrès du Groupe Français de Bioénergétique, Sep 2021, online seminars, France
Conference papers hal-03705957v1

The role of chloroplastic NFU in the green alga Chlamydomonas reinhardtii

Jonathan Przybyla-Toscano , Claire Remacle , Nicolas Rouhier
Online seminar series on “Iron-sulfur protein biogenesis 2021”, Jun 2021, e-seminar, France
Conference papers hal-03298704v1

Maturation of Fe-S proteins in plant organelles.

Nicolas Rouhier
12th congress of the French Society of Plant Biology, Jul 2019, Strasbourg, France
Conference papers hal-03197997v1

Sulfur trafficking and H2S-mediated redox signaling in plants: roles of Arabidopsis 3-MP sulfurtransferases

Tiphaine Dhalleine , Anna Moseler , Christophe H Marchand , Stéphane D. Lemaire , Nicolas Rouhier
SPP1710 Conference Thiol-based switches and redox regulation – from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain
Conference papers hal-03000210v1

Are the ubiquitous mitochondrial glutaredoxin-like proteins of 12 kDa (MGP12) true glutaredoxins?

Tiphaine Dhalleine , Mathieu Schwartz , Marc Quinternet , Benjamin Chagot , Benjamin Petre
Thiol-based switches and redox regulation – from microbes to men, Sep 2019, San Feliu de Guixols, Spain
Conference papers hal-03197996v1

Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition

Kevin Robe , Geneviève Conejero , Nicolas Tissot , Fei Gao , Nathalie Berger
XVIII International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan
Conference papers hal-02952560v1

Functional characterization of mitochondrial NFU proteins in plants

Jonathan Przybyla-Toscano , Andrew Mclean , Tamanna Azam , Cyril Magno , Tiphaine Dhalleine
8th International Conference on Iron-Sulfur Cluster Biogenesis and Regulation, May 2018, Madison, United States
Conference papers hal-02955576v1

Deciphering the biochemical properties of bacterial rhodanese fusion proteins.

Mathilde Hériché , Tiphaine Dhalleine , Nicolas Rouhier , Jérémy Couturier
Fourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany
Conference papers hal-03000211v1

The maturation of Fe-S proteins in chloroplasts and mitochondria

Jonathan Przybyla-Toscano , Andrew Mclean , Tamanna Azam , Cyril Magno , Tiphaine Dhalleine
Jacques Monod conference “Retrograde signalling from endosymbiotic organelles”, Oct 2018, Roscoff, France
Conference papers hal-02955303v1

Elucidating the function of the chloroplastic glutaredoxin S16 of Arabidopsis thaliana.

Nicolas Rouhier , Flavien Zannini , Sowmya Subramanian , Anna-Maria Moseler , Andreas Meyer
12th Congress of the International Plant Molecular Biology, Aug 2018, Montpellier, France
Conference papers hal-03198404v1

Arabidopsis thaliana GRXS16, a multifaceted glutaredoxin in chloroplasts.

Flavien Zannini , Anna Moseler , Andreas J Meyer , Nicolas Rouhier , Jérémy Couturier
3rd Redox Biology Symposium, Redox regulation: Historical background and future developments, Mar 2017, Nancy, France
Conference papers hal-03000213v1

Who are the partners of mitochondrial Arabidopsis thaliana GRXS15 in trafficking of iron-sulfur clusters?

Jonathan Przybyla-Toscano , Anna Moseler , Marta A. Uzarska , Ulrich Mühlenhoff , Jérémy Couturier
Redox meeting and Conversation, Mar 2017, Vandoeuvre Lès Nancy, France
Conference papers hal-04176965v1

The chloroplastic glutaredoxin S16: a plant-specific, two-domain protein with multiple biochemical properties.

Flavien Zannini , Nicolas Rouhier , Jérémy Couturier
EMBO Conference on Redox Biology, Jul 2017, Moscou, Russia
Conference papers hal-03198418v1

How are Fe-S cofactors and proteins assembled in plant cells? focus on NFUs, involved in the late steps of the maturation process in organelles

Brigitte Touraine , Jonathan Przybyla-Toscano , Nathalie Berger , Jérémy Couturier , Frédéric Gaymard
Days of the French society of photosynthesis, Jun 2017, Paris, France
Conference papers hal-02952587v1

Deciphering the molecular mechanisms of sulfur trafficking in plants.

Tiphaine Dhalleine , Pascale Tsan , Christophe H Marchand , Stéphane D.Lemaire , Nicolas Rouhier
hird meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Jul 2016, Düsseldorf, Germany
Conference papers hal-03000214v1

Deciphering the late-steps of FeS cluster assembly in plant organelles: focus on NFUs.

Jonathan Przybyla-Toscano , Brigitte Touraine , Mélanie Roland , Florence Vignols , Jérémy Couturier
Kick off meeting of the COST action CA15133 FeSBioNET “The biogenesis of iron-sulfur proteins: from cellular biology to molecular aspects”., Sep 2016, Patras, Greece
Conference papers hal-03198428v1

Plastidial Nfu2 and Nfu3 are crucial for a proper root and shoot development in Arabidopsis thaliana

Brigitte Touraine , Hunyao Gao , Hui-Chen Wu , Przybyla-Toscano Jonathan , Sowmya Subramanian
7th International Conference on Iron-Sulfur Cluster Biogenesis and Regulation., Jun 2015, bergame, Italy
Conference papers hal-03198457v1

Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexes

Thomas Roret , Jérémy Couturier , Pascale Tsan , Bo Zhang , Jean-Pierre Jacques
IUBMB Symposium FeS 2015 - Iron Sulfur Cluster Biogenesis and Regulation, Jun 2015, Bergamo, Italy
Conference papers hal-03000372v1

Structure-function analyses of plant glutathione transferases with catalytic cysteine residues

Nicolas Rouhier
Theoretical and Practical Course “Redox Chemistry and Biology of Thiols” and associated symposium., Mar 2015, montevideo, Uruguay
Conference papers hal-03198451v1

The highly expanded thioredoxin and glutaredoxin classes in plants: specificities in the regeneration systems of enzymes using a sulfenic acid chemistry

Nicolas Rouhier
Theoretical and Practical Course “Redox Chemistry and Biology of Thiols” and associated symposium, Mar 2015, Montevideo, Uruguay
Conference papers hal-03198450v1

Structure-function analysis of cysteine-containing glutathione transferases (GSTs) from Populus trichocarpa

Pierre-Alexandre Lallement , Henri Pégeot , Arnaud Hecker , Nicolas Rouhier
Thiol-based redox switches in life sciences, EMBO-ESF meeting, Sep 2015, san feliu de guixols, Spain
Conference papers hal-03198459v1

Exploring the partners of late-acting Fe-S transfer proteins belonging to the mitochondrial ISC machinery in Arabidopsis thaliana.

Jonathan Przybyla-Toscano , Jean-Pierre Jacquot , Jérémy Couturier , Florence Vignols , Nicolas Rouhier
Labex Mitocross 2015: mitochondria at the crossroad, Sep 2015, Strasbourg, France
Conference papers hal-03198462v1

The multiple facets of glutaredoxins: one fold, several partners and functions

Jérémy Couturier , Mirko Zaffagnini , Elke Ströher , Angela-Nadia Albetel , Bo Zhang
12th International Conference on Reactive Oxygen and Nitrogen Species in Plants: from model systems to field, Jun 2015, verone, Italy
Conference papers hal-03198452v1

Monothiol glutaredoxin and BolA in plants: two proteins with multiple interaction modes.

Jérémy Couturier , Hui-Chen Wu , Thomas Roret , Pascale Tsan , Tiphaine Dhalleine
9th International Workshop on Sulfur Metabolism in Plants: Molecular Physiology and Ecophysiology of Sulfur, Apr 2014, Freiburg, Germany
Conference papers hal-03000377v1

Biochemical, spectroscopic and structural insights into glutaredoxins and their multiple types of interactions with BolAs

Nicolas Rouhier
2nd Symposium of the DFG Priority Program SPP 1710 “Dynamics of Thiol-based Redox Switches in Cellular Physiology”, Sep 2014, Heidelberg, Germany
Conference papers hal-03198444v1

Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexes.

Jérémy Couturier , Thomas Roret , Hui-Chen Wu , Pascale Tsan , Tiphaine Dhalleine
Gordon research conference “Thiol-based redox regulation and signalling”., Jul 2014, girone, Spain
Conference papers hal-03198441v1

Biochemical, spectroscopic and structural insights into BolA-glutaredoxin complexes.

Jérémy Couturier , Thomas Roret , Hui-Chen Wu , Pascale Tsan , Tiphaine Dhalleine
IJB Symposium «Molecular Sciences, Facing Up to Major Societal Challenges»., Oct 2014, Nancy, France
Conference papers hal-03198448v1

Genomics, transcriptomics and effectoromics of the poplar leaf rust fungus Melampsora larici-populina

Benjamin Petre , Stéphane S. Hacquard , Antoine Persoons , Emilie Tisserant , Emmanuelle Morin
IUFRO Joint Meeting on Genetics of Five-Needle Pines and Rusts in Mountain Landscapes, Jun 2014, Fort Collins, United States
Conference papers hal-02801804v1

Plastidial glutaredoxins: glutathione-dependent enzymes involved in detoxification, redox signalling and iron-sulfur cluster biogenesis.

Jérémy Couturier , Mirko Zaffagnini , Elke Ströher , Angela-Nadia Albetel , Thomas Roret
9th International Workshop - Sulfur Metabolism in Plants., Apr 2014, freiburg, Germany
Conference papers hal-03198438v1

Investigation of the role of glutaredoxins and NFU proteins for the plastidial iron-sulfur cluster assembly machinary

Brigitte Touraine , Pascal Rey , Maryline Lièvre , Hui-Chen Wu , Hunyao Gao
5th International Meeting of the Institute of Metals in Biology of Grenoble (IMBG)/Metal Homeostasis, Sep 2012, Autrans, France
Conference papers hal-02952861v1

Les apports de la génomique pour l’étude des familles multigéniques : exemple des thaumatin-like proteins (TLPs) chez le peuplier

Benjamin Petre , Ian Major , Pascal Frey , Francis Martin , Nicolas Rouhier
Séminaire de l’Ecole Doctorale RP2E. Biodiversité, Ingénierie écologique, Jan 2011, Vandoeuvre-lès-Nancy, France. 8 p
Conference papers hal-02746248v1

The complex redox regulation of the SAL1 phosphatase present in plant organelles

Tiphaine Dhalleine , Natacha Donnay , Linda de Bont , Nicolas Rouhier
Thiol oxidation in biology: Biochemical mechanisms to physiological outcomes, Oct 2022, sant feliu de guixols, Spain
Conference poster hal-03820252v1

Maturation of iron-sulfur-containing subunits of respiratory complex I in plants

Alicia López-López , Jonathan Przybyla-Toscano , Olivier Keech , Nicolas Rouhier
Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting, Sep 2022, Sainte Maxime, France
Conference poster hal-03781848v1

Iron-sulfur cluster formation and transfer using plant mitochondrial glutaredoxin-BOLA complexes.

Loïck Christ , Jérémy Couturier , Nicolas Rouhier
Iron Sulfur Proteins: Biogenesis, Regulation and Function meeting., Sep 2022, Sainte-maxime, France
Conference poster hal-03820255v1

Exploiting a bacterial cysteine desulfurase-sulfurtransferase fusion to develop a roGFP2-based biosensor for free cysteine

Damien Caubrière , Benjamin Selles , Anna Moseler , Tiphaine Dhalleine , Morgane Ziesel
Redox Biology Congress 2022 Oxidative stress, redox biology and antioxidants from plants to humans, Aug 2022, Ghent, Belgium
Conference poster hal-03775303v1

Sulfurtransferase-mediated formation of H2S and persulfidation in Arabidopsis thaliana

Anna Moseler , Tiphaine Dhalleine , Andreas J. Meyer , Nicolas Rouhier , Jérémy Couturier
Botanik-Tagung International Conference of the German Society for Plant Sciences, Aug 2022, Bonn, Germany
Conference poster hal-03775320v1

Unravelling the role of an atypical mitochondrial ferredoxin from Arabidopsis thaliana.

Jonathan Przybyla-Toscano , Röhricht Hélène , Olivier Keech , Etienne H Meyer , Nicolas Rouhier
Congrès du Groupe Français de Bioénergétique, Sep 2021, online seminar, France
Conference poster hal-03705964v1

Identification of client iron-sulfur proteins of the chloroplastic NFU3 transfer protein from Chlamydomonas reinhardtii.

Jonathan Przybyla-Toscano , Véronique Larosa , Claire Remacle , Nicolas Rouhier
International Bioenergy and environment congress, Feb 2021, online seminar, France
Conference poster hal-03707813v1

Shedding light on a H2S generating pathway in Arabidopsis

Anna Moseler , Tiphaine Dhalleine , Nicolas Rouhier , Jérémy Couturier
S-Bio 2021 Glucosinolate Joint meeting for plant and human sulfur biology and glucosinolates, Sep 2021, Seville, Spain
Conference poster hal-03358437v1

Investigation of cellular interactions and biochemical characterization of the chloroplastic NFU1 transfer protein from Chlamydomonas reinhardtii.

Jonathan Przybyla-Toscano , Leo Noppe , Claire Remacle , Nicolas Rouhier
Chlamy 2020+1. 19th International Conference on the Cell and Molecular Biology of Chlamydomonas., Aug 2021, Six-Fours-les-Plages, France
Conference poster hal-03707828v1

Deciphering the biochemical relationships between cysteine desulfurase and sulfurtransferase protein families in sulfur trafficking

Damien Caubrière , Benjamin Selles , Anna Moseler , Morgane Ziesel , Dhalleine Tiphaine
S-Bio 2021 Glucosinolate Joint meeting for plant and human sulfur biology and glucosinolates, Sep 2021, Seville, Spain
Conference poster hal-03358427v1

Does Arabidopsis sulfurtransferase 18 participate to sulfur trafficking?

Benjamin Selles , Tiphaine Dhalleine , Nicolas Rouhier , Jérémy Couturier
Thiol-based switches and redox regulation - from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain
Conference poster hal-02991609v1

Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition

Kevin Robe , Geneviève Conejero , Nicolas Tissot , Fei Gao , Nathalie Berger
12ème Congrès de la Société Française de Biologie Végétale SFBV, Jul 2019, Strasbourg, France
Conference poster hal-02951139v1

Developing redox biosensors for sulfur compounds

Anna Moseler , Bruce Morgan , Nicolas Rouhier , Jérémy Couturier
SPP1710 Conference Thiol-based switches and redox regulation – from microbes to men, Sep 2019, Sant Feliu de Guixols, Spain
Conference poster hal-03000388v1

The α-amylase 3 of Arabidopsis thaliana is protected by S-glutathionylation in vitro

Libero Gurrieri , Luca Distefano , Claudia Pirone , Daniel Horrer , David Seung
114° Congresso della Società Botanica Italiana onlus, VI INTERNATIONAL PLANT SCIENCE CONFERENCE (IPSC)., Sep 2019, padova, Italy
Conference poster hal-03298595v1

Deciphering the biochemical properties of plastidial GRXS14 and mitochondrial GRXS15 isoforms of Arabidopsis thaliana

Anna Moseler , Nicolas Rouhier , Jérémy Couturier
Fourth meeting of the study group redox biology of the German Society for Biochemistry and Molecular Biology, Sep 2018, Berlin, Germany
Conference poster hal-03158701v1

Dynamics of coumarin secretion by plant roots into the soil to improve iron nutrition

Kevin Robe , Geneviève Conejero , Nicolas Tissot , Fei Gao , Nathalie Berger
XVIII International Symposium on Iron Nutrition and Interactions in Plants, Jul 2018, Taipei, Taiwan
Conference poster hal-02951465v1

Decrypting the chloroplastic [fe-s] cluster assembly machinery

Nathalie Berger ,