Nicolas Soler

Nicolas SOLER, PhD Associate professor at the Université de Lorraine, Nancy, France DynAMic Unit UMR1128 UL-INRAE
23
Documents
Affiliations actuelles
  • Université de Lorraine (UL)
  • Faculté des Sciences et Technologies [Université de Lorraine] (FST)
  • Dynamique des Génomes et Adaptation Microbienne (DynAMic)
  • Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
  • Département Microbiologie et Chaîne Alimentaire - INRAE (MICA)
  • Dynamique des Génomes et Adaptation Microbienne (DynAMic)
Identifiants chercheurs
Contact

Présentation

Biography I carried out my PhD in microbiology at the Université Paris-Sud (now Université Paris-Saclay), in professor Patrick Forterre’s laboratory. My work, defended in 2007, focused on searching and characterizing mobile genetic elements from hyperthermophilic archaea. This raised my interest in molecular microbiology and the evolution of genomes. I was then recruited by the CNRS from 2007 to 2010, in G Baldacci’s team in Institut Curie, in order to work on 2 essential proteins in yeast in connection with DNA replication. Next, I joined Dr Stéphane Emiliani’s lab at the Institut Cochin in Paris for 3 years to study the early steps of the HIV-1 cycle. Since September 2013, I have been working in the DynAMic lab at the University of Lorraine. There, I am studying the molecular aspects of conjugation in Gram+ bacteria, in the team headed by Pr Nathalie Leblond-Bourget.

Research My progress in research enabled me to discover several aspects of biology; indeed, I worked successively on archaea, eucaryotes, a virus, and bacteria, still focusing on micro-organisms and their evolution. During my PhD, I looked for mobile genetic elements, plasmids and viruses, from hyperthermophilic archaea. These pioneering works on Thermococcales led to the discovery of new families of plasmids; consequently, new genetic tools were implemented for this arising phylum. I also highlighted a massive production of membrane vesicles from these archaea, which have since then been involved in horizontal gene transfer. My skills in protein biochemistry enabled me to integrate the Institut Curie where I worked on the biochemical and biophysical characterization of two essential proteins in yeast. These proteins, conserved in eucaryotes, are involved in the biogenesis of the Fe/S clusters in connection with DNA replication. I was then recruited by Institut Cochin to characterize multiprotein complexes, which form during the early steps of lymphocyte infection by HIV-1. I especially focused on viral integrase and how it interacts with other viral or cellular partners. Since I was recruited at the Unviversité de Lorraine, I have been developing a new topic aiming at characterizing the first steps of bacterial conjugation in streptococcus using an ICE model, with molecular and structural approaches. Over the last few years, it has been demonstrated that ICEs are prevalent in bacterial genomes and that they are involved in the transfer of both antibiotic resistance and virulence genes. Characterization of their transfer at the molecular level may one day give insights to limit antibiotic resistance genes to spread. Morevover, whereas some aspects of conjugation are now well understood in proteobacteria, very little data is today available in Firmicutes. Yet, they harbor a lot of multiresistant strains which threaten public health. Thus, when I arrived in DynAMic lab, I was immediately attracted by ICEs as an emerging study topic in Gram+ bacteria.

Teaching Since 2013, I have been teaching formal genetics, general microbiology and microbial ecology at a bachelor level as an associate professor at the University of Lorraine. I also teach in molecular microbiology and virology within the Microbiology master degree. Moreover, since 2022 I am responsible of a training in biotechnology engineering, the “Cursus Master en Ingénierie Biotechnologies en Microbiologie et Ingénierie Moléculaire” (BioMIM) of the Université de Lorraine. This training is a five-year training based on a bachelor and a master degree with a special focus on project-based learning.

Domaines de recherche

Biochimie [q-bio.BM] Biologie moléculaire Biophysique Biologie structurale [q-bio.BM] Organisation et fonctions cellulaires [q-bio.SC] Evolution [q-bio.PE] Systématique, phylogénie et taxonomie Bactériologie

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Publications

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Massive acquisition of conjugative and mobilizable integrated elements fuels Faecalibacterium plasticity and hints at their adaptation to the gut

Gérard Guédon , Florence Charron-Bourgoin , Thomas Lacroix , Toufik Hamadouche , Nicolas Soler et al.

Scientific Reports, 2025, 15 (1), pp.17013. ⟨10.1038/s41598-025-99981-y⟩

Article dans une revue hal-05397378v1
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Two OB-fold proteins from a Gram-positive conjugative element engage in relaxosome assembly and DNA processing

Haifa Laroussi , Robine Maffo-Woulefack , Julien Cappèle , Hicham Sekkouri Alaoui , Lysiane Lessure et al.

Nucleic Acids Research, 2025, 53 (21), pp.gkaf1161. ⟨10.1093/nar/gkaf1161⟩

Article dans une revue hal-05445247v1
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Exploration of DNA processing features unravels novel properties of ICE conjugation in Gram-positive bacteria

Haifa Laroussi , Yanis Aoudache , Emilie Robert , Virginie Libante , Louise Thiriet et al.

Nucleic Acids Research, 2022, 50 (14), pp.8127-8142. ⟨10.1093/nar/gkac607⟩

Article dans une revue hal-03881644v1
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Des vésicules pour échanger de l’ADN

Nicolas Soler , Patrick Forterre

Médecine/Sciences, 2021, 37 (6-7), pp.583-585. ⟨10.1051/medsci/2021070⟩

Article dans une revue hal-03273134v1
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Extracellular membrane vesicles and nanotubes in Archaea

Junfeng Liu , Nicolas Soler , Aurore Gorlas , Virginija Cvirkaite-Krupovic , Mart Krupovic et al.

microLife, 2021, 2, pp.uqab007. ⟨10.1093/femsml/uqab007⟩

Article dans une revue pasteur-03264764v2

Vesiduction: the fourth way of HGT

Nicolas Soler , Patrick Forterre

Environmental Microbiology, 2020, ⟨10.1111/1462-2920.15056⟩

Article dans une revue hal-02571417v1
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Characterization of a relaxase belonging to the MOBT family, a widespread family in Firmicutes mediating the transfer of ICEs

Nicolas Soler , Emilie Robert , Isaure Chauvot de Beauchêne , Philippe Monteiro , Virginie Libante et al.

Mobile DNA, 2019, 10 (1), pp.1-16. ⟨10.1186/s13100-019-0160-9⟩

Article dans une revue hal-02138843v1
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Membrane vesicles in natural environments: a major challenge in viral ecology.

Nicolas Soler , Mart Krupovic , Evelyne Marguet , Patrick Forterre

The International Society of Microbiologial Ecology Journal, 2015, 9 (4), pp.793-6. ⟨10.1038/ismej.2014.184⟩

Article dans une revue hal-01436521v1

Plasmids from Euryarchaeota.

Patrick Forterre , Mart Krupovic , Kasie Raymann , Nicolas Soler

Microbiology Spectrum, 2014, 2 (6), pp.27. ⟨10.1128/microbiolspec.PLAS-0027-2014⟩

Article dans une revue hal-01477453v1
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Identification of low molecular weight nuclear complexes containing integrase during the early stages of HIV-1 infection.

Annabelle Gérard , Nicolas Soler , Emmanuel Ségéral , Michael Belshan , Stéphane Emiliani

Retrovirology, 2013, 10 (1), pp.13. ⟨10.1186/1742-4690-10-13⟩

Article dans une revue inserm-00788098v1

Fake virus particles generated by fluorescence microscopy.

Patrick Forterre , Nicolas Soler , Mart Krupovic , Evelyne Marguet , Hans-W Ackermann

Trends in Microbiology, 2013, 21 (1), pp.1-5. ⟨10.1016/j.tim.2012.10.005⟩

Article dans une revue istex hal-00779641v1

Plasmids, viruses and virus-like membrane vesicles from Thermococcales.

Nicolas Soler , Marie Gaudin , Evelyne Marguet , Patrick Forterre

Biochemical Society Transactions, 2011, 39 (1), pp.36-44. ⟨10.1042/BST0390036⟩

Article dans une revue hal-00596065v1

Interaction between the reductase Tah18 and highly conserved Fe-S containing Dre2 C-terminus is essential for yeast viability.

Nicolas Soler , Emmanuelle Delagoutte , Simona Miron , Céline Facca , Dorothée Baïlle et al.

Molecular Microbiology, 2011, 82 (1), pp.54-67. ⟨10.1111/j.1365-2958.2011.07788.x⟩

Article dans une revue hal-00634151v1
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Two novel families of plasmids from hyperthermophilic archaea encoding new families of replication proteins

Nicolas Soler , Evelyne Marguet , Diego Cortez , Nicole Desnoues , Jenny Keller et al.

Nucleic Acids Research, 2010, 38 (15), pp.5088-5104. ⟨10.1093/nar/gkq236⟩

Article dans une revue hal-04450616v1
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A Newly Identified Essential Complex, Dre2-Tah18, Controls Mitochondria Integrity and Cell Death after Oxidative Stress in Yeast

Laurence Vernis , Céline Facca , Emmanuelle Delagoutte , Nicolas Soler , Roland Chanet et al.

PLoS ONE, 2009, 4 (2), pp.e4376. ⟨10.1371/journal.pone.0004376⟩

Article dans une revue hal-01625408v1

A protein encoded by a new family of mobile elements from Euryarchaea exhibits three domains with novel folds.

J. Keller , N. Leulliot , N. Soler , B. Collinet , R. Vincentelli et al.

Protein Science, 2009, 18 (4), pp.850-5. ⟨10.1002/pro.73⟩

Article dans une revue hal-00529493v1

Virus-like vesicles and extracellular DNA produced by hyperthermophilic archaea of the order Thermococcales.

Nicolas Soler , Evelyne Marguet , Jean-Marc Verbavatz , Patrick Forterre

Research in Microbiology, 2008, 159 (5), pp.390-9. ⟨10.1016/j.resmic.2008.04.015⟩

Article dans une revue istex hal-00356456v1

The rolling-circle plasmid pTN1 from the hyperthermophilic archaeon Thermococcus nautilus.

Nicolas Soler , Anthony Justome , Sophie Quevillon-Cheruel , Florence Lorieux , Eric Le Cam et al.

Molecular Microbiology, 2007, 66 (2), pp.357-70. ⟨10.1111/j.1365-2958.2007.05912.x⟩

Article dans une revue hal-00192504v1

Plasmids from Euryarchaeota

Patrick Forterre , Mart Krupovic , Kasie Raymann , Nicolas Soler

Marcelo E. Tolmasky, Juan C. Alonso. Plasmids: Biology and Impact in Biotechnology and Discovery, ASM Press, pp.718, 2015, 9781555818975

Chapitre d'ouvrage hal-01478643v1