Access content directly

Richard GALINIER

48
Documents
Researcher identifiers
  • IdHAL richard-galinier
  • ResearcherId : G-8278-2011
  • ORCID 0000-0002-4180-9303
  • Google Scholar : https://scholar.google.com/citations?user=n4UMqRsAAAAJ&hl=en&oi=ao
  • IdRef : 081056109
  • ResearcherId : http://www.researcherid.com/rid/G-8278-2011

Presentation

Je pilote des projets de recherche portant sur les interactions immunobiologiques au sein des systèmes hôte/pathogène. Plusieurs objectifs guident ces projets : a) l’identification et de caractérisation des molécules clés de la virulence du pathogène et de la résistance de l’hôte, b) décortiquer les mécanismes de l’immunité chez les invertébrés et les replacer dans un contexte évolutif, c) lutter contre les infections relatives aux pathogènes étudiés. Nos approches expérimentales consistent principalement en des analyses comparatives d’expression de gènes et de protéines impliquées dans ces interactions en fonction de conditions environnementales changeantes, et cela tant au niveau des individus que des populations. Je suis également intéressé par la caractérisation fonctionnelle des protéines candidates ainsi identifiées. Je maitrise un large panel de techniques de biologie moléculaire et de biochimie. Pour la partie transcriptomique: extraction d’ADN, d’ARN et de protéines, PCR, clonage, mutagénèse dirigée, PCR quantitative, construction de banques EST, de banques de micorsatellites, de banques soustractives, hybridation *in situ*, mais également analyse de séquençage Sanger et de séquençage massif de transcriptome (RNA-seq), analyse de profil microsatellite en multiplex. Pour la partie protéomique : électrophorèse 1D de protéines, Western blot, immunoprécipitation, expression recombinante de protéines et leur purification, approches d’interactome. Pour plus d'informations: <http://ihpe.univ-perp.fr/ihpe-galinier-richard/>
I am leading research projects dealing with immunobiological interactions in host/pathogen systems. Goals are a) the identification and characterization of key molecules involved in pathogen virulence and host resistance, b) to decipher the mechanisms of invertebrate immunity at the molecular and evolution levels, c) to fight against the disease caused by studied pathogens. Our experimental approaches are mainly comparative analyses of genes and proteins expression between different environmental or experimental conditions, at both individual and population levels. I am also interested by the functional validation of candidate protein identified. My expertise domain concern transcriptomic and proteomic approaches. At the transcriptomic level: nucleic acid extraction, PCR, cloning, mutagenesis, real time PCR, library construction (EST, microsatellite, substractive,…), in situ hybridization, RNA-Seq and Sanger sequencing analyses, microsatellite multiplex analyses. At the proteomic level: protein 1D electrophoresis, western blot, immunoprecipitation, heterologous and cell free expression system, protein purification system, interactome approach. For more information: <http://ihpe.univ-perp.fr/en/ihpe-galinier-richard-english/>

Publications

Image document

Single cell RNA sequencing reveals hemocyte heterogeneity in Biomphalaria glabrata: Plasticity over diversity

Remi Pichon , Silvain Pinaud , Emmanuel Vignal , Cristian Chaparro , Marine Pratlong
Frontiers in Immunology, 2022, 13, ⟨10.3389/fimmu.2022.956871⟩
Journal articles hal-03772193v1
Image document

Genetic Diversity and Connectivity of the Ostreid Herpesvirus 1 Populations in France: A First Attempt to Phylogeographic Inference for a Marine Mollusc Disease

Jean Delmotte , Camille Pelletier , Benjamin Morga , Richard Galinier , Bruno Petton
Virus Evolution, 2022, 8 (1), pp.veac039. ⟨10.1093/ve/veac039⟩
Journal articles hal-03652456v1
Image document

Hemocyte siRNA uptake is increased by 5′ cholesterol-TEG addition in Biomphalaria glabrata , snail vector of schistosome

Anaïs Portet , Richard Galinier , Damien Lassalle , Alexandre Faille , Benjamin Gourbal
PeerJ, 2021, 9, pp.e10895. ⟨10.7717/peerj.10895⟩
Journal articles hal-03163875v1
Image document

Experimental Infection of the Biomphalaria glabrata Vector Snail by Schistosoma mansoni Parasites Drives Snail Microbiota Dysbiosis

Anaïs Portet , Eve Toulza , Ana Lokmer , Camille Huot , David Duval
Microorganisms, 2021, 9 (5), pp.1084. ⟨10.3390/microorganisms9051084⟩
Journal articles hal-03315096v1
Image document

New insights into biomphalysin gene family diversification in the vector snail Biomphalaria glabrata

Silvain Pinaud , Guillaume Tetreau , Pierre Poteaux , Richard Galinier , Cristian Chaparro
Frontiers in Immunology, 2021, 12, pp.635131. ⟨10.3389/fimmu.2021.635131⟩
Journal articles hal-03219003v1
Image document

A New Assessment of Thioester-Containing Proteins Diversity of the Freshwater Snail Biomphalaria glabrata

David Duval , Remi Pichon , Damien Lassalle , Maud Laffitte , Benjamin Gourbal
Journal articles hal-02431935v1
Image document

Contribution of Viral Genomic Diversity to Oyster Susceptibility in the Pacific Oyster Mortality Syndrome

Jean Delmotte , Cristian Chaparro , Richard Galinier , Julien de Lorgeril , Bruno Petton
Frontiers in Microbiology, 2020, 11, pp.1579. ⟨10.3389/fmicb.2020.01579⟩
Journal articles hal-02915023v1
Image document

Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor

Guillaume Tetreau , Julien Dhinaut , Richard Galinier , Pascaline Audant-Lacour , Sébastien Voisin
PLoS Pathogens, 2020, 16 (10), pp.e1008935. ⟨10.1371/journal.ppat.1008935⟩
Journal articles hal-02994654v1
Image document

Schistosomiasis vector snails and their microbiota display a phylosymbiosis pattern

Camille Huot , Camille Clerissi , Benjamin Gourbal , Richard Galinier , David Duval
Frontiers in Microbiology, inPress, ⟨10.3389/fmicb.2019.03092⟩
Journal articles hal-02440210v1
Image document

Glabralysins, Potential New β-Pore-Forming Toxin Family Members from the Schistosomiasis Vector Snail Biomphalaria glabrata

Damien Lassalle , Guillaume Tetreau , Silvain Pinaud , Richard Galinier , Neil Crickmore
Journal articles hal-02431962v1
Image document

The immunobiological interplay between Pseudosuccinea columella resistant/susceptible snails with Fasciola hepatica: Hemocytes in the spotlight

Annia Alba , David Duval , Jorge Sánchez , Ana B Perez , Silvain Pinaud
Developmental and Comparative Immunology, 2020, 102, pp.103485. ⟨10.1016/j.dci.2019.103485⟩
Journal articles hal-02279344v1
Image document

Sympatric versus allopatric evolutionary contexts shape differential immune response in Biomphalaria / Schistosoma interaction

Anaïs Portet , Silvain Pinaud , Cristian Chaparro , Richard Galinier , Nolwenn M. Dheilly
PLoS Pathogens, 2019, 15 (3), pp.e1007647. ⟨10.1371/journal.ppat.1007647⟩
Journal articles hal-02076267v1
Image document

Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata

Silvain Pinaud , Anaïs Portet , Jean-François Allienne , Lucid Belmudes , Cécile Saint-Beat
Developmental and Comparative Immunology, 2019, 92, pp.238-252. ⟨10.1016/j.dci.2018.12.001⟩
Journal articles hal-01952235v1
Image document

BgTEP: an antiprotease involved in innate immune sensing in Biomphalaria glabrata

Anaïs Portet , Richard Galinier , Silvain Pinaud , Julien Portela , Fanny Nowacki
Frontiers in Immunology, 2018, 9, pp.1206. ⟨10.1101/308130⟩
Journal articles hal-01838951v1
Image document

Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters

Julien de Lorgeril , Aude Lucasson , Bruno Petton , Eve Toulza , Caroline Montagnani
Nature Communications, 2018, 9 (1), pp.4215. ⟨10.1038/s41467-018-06659-3⟩
Journal articles hal-01898110v1
Image document

Whole genome analysis of a schistosomiasis-transmitting freshwater snail

C. Adema , L. Hillier , C. Jones , E.S. Loker , M. Knight
Nature Communications, 2017, 8, pp.15451. ⟨10.1038/ncomms15451⟩
Journal articles hal-01523953v1
Image document

Specific Pathogen Recognition by Multiple Innate Immune Sensors in an Invertebrate

Guillaume Tetreau , Silvain Pinaud , Anaïs Portet , Richard Galinier , Benjamin Gourbal
Frontiers in Immunology, 2017, 8, pp.1249. ⟨10.3389/fimmu.2017.01249⟩
Journal articles hal-01729395v1
Image document

Oxidative stress induced by glyphosate-based herbicide on freshwater turtles

Laurent Héritier , David Duval , Richard Galinier , Anne-Leila Meistertzheim , Olivier Verneau
Environmental Toxicology and Chemistry, 2017, 36 (12), pp.3343 - 3350. ⟨10.1002/etc.3916⟩
Journal articles hal-01808555v1
Image document

First characterization of viruses from freshwater snails of the genus Biomphalaria, the intermediate host of the parasite Schistosoma mansoni

Richard Galinier , Guillaume Tetreau , Anaïs Portet , Silvain Pinaud , David Duval
Acta Tropica, 2017, 167, pp.196-203. ⟨10.1016/j.actatropica.2016.12.021⟩
Journal articles hal-01435053v1
Image document

Integrated multi-omic analyses in Biomphalaria-Schistosoma dialogue reveal the immunobiological significance of FREP- Sm PoMuc interaction

Anaïs Portet , Silvain Pinaud , Guillaume Tetreau , Richard Galinier , Celine Cosseau
Developmental and Comparative Immunology, 2017, 75, pp.16 - 27. ⟨10.1016/j.dci.2017.02.025⟩
Journal articles hal-01598455v1
Image document

A multistrain approach to studying the mechanisms underlying compatibility in the interaction between Biomphalaria glabrata and Schistosoma mansoni

Richard Galinier , Emmanuel Roger , Yves Moné , David Duval , Anaïs Portet
PLoS Neglected Tropical Diseases, 2017, 11 (3), pp.e0005398. ⟨10.1371/journal.pntd.0005398.s005⟩
Journal articles hal-01511153v1
Image document

Outbreak of urogenital schistosomiasis in Corsica (France): an epidemiological case study

Jérôme Boissier , Sébastien Grech-Angelini , B. L. Webster , Jean-François Allienne , Tine Huyse
The Lancet Infectious Diseases, 2016, 16 (8), pp.971-979. ⟨10.1016/S1473-3099(16)00175-4⟩
Journal articles hal-01364002v1
Image document

A shift from cellular to humoral responses contributes to innate immune memory in the vector snail $Biomphalaria\ glabrata$

Silvain Pinaud , Julien Portela , David Duval , Fanny Nowacki , Marie-Aude Olive
PLoS Pathogens, 2016, 12 (1), pp.e1005361. ⟨10.1371/journal.ppat.1005361⟩
Journal articles hal-03778962v1
Image document

A Novel Bacterial Pathogen of Biomphalaria glabrata: A Potential Weapon for Schistosomiasis Control?

David Duval , Richard Galinier , Gabriel Mouahid , Eve Toulza , Jean-François Allienne
PLoS Neglected Tropical Diseases, 2015, 9 (2), pp.e0003489. ⟨10.1371/journal.pntd.0003489.s002⟩
Journal articles hal-01162649v1
Image document

A family of variable immunoglobulin and lectin domain containing molecules in the snail Biomphalaria glabrata

Nolwenn M. Dheilly , David Duval , Gabriel Mouahid , Rémi Emans , Jean-François Allienne
Developmental and Comparative Immunology, 2015, 48 (1), pp.234-243. ⟨10.1016/j.dci.2014.10.009⟩
Journal articles hal-01082870v1
Image document

Who is the puppet master? Replication of a parasitic wasp-associated virus correlates with host behaviour manipulation

Nolwenn M. Dheilly , Fanny Maure , Marc Ravallec , Richard Galinier , Josée Doyon
Proceedings of the Royal Society B: Biological Sciences, 2015, 282 (1803), pp.20142773. ⟨10.1098/rspb.2014.2773⟩
Journal articles hal-01141139v1
Image document

Evidence for Specific Genotype-Dependent Immune Priming in the Lophotrochozoan Biomphalaria glabrata Snail.

Julien Portela , David Duval , Anne Rognon , Richard Galinier , Jérôme Boissier
Journal of Innate Immunity, 2013, 5 (3), pp.261-276. ⟨10.1159/000345909⟩
Journal articles halsde-00790554v1
Image document

Biomphalysin, a new β pore-forming toxin involved in Biomphalaria glabrata immune defense against Schistosoma mansoni.

Richard Galinier , Julien Portela , Yves Moné , Jean-François Allienne , Hélène Henri
PLoS Pathogens, 2013, 9 (3), pp.e1003216. ⟨10.1371/journal.ppat.1003216⟩
Journal articles halsde-00823685v1

Genetic diversity, fixation and differentiation of the freshwater snail Biomphalaria pfeifferi (Gastropoda, Planorbidae) in arid lands.

Rodrigue Mintsa Nguema , Juliette Langand , Richard Galinier , Mohamed A Idris , Mahmoud A Shaban
Genetica, 2013, 141, pp.171-184. ⟨10.1007/s10709-013-9715-8⟩
Journal articles halsde-00823688v1

Complete genomic sequence and taxonomic position of eel virus European X (EVEX), a rhabdovirus of European eel.

Richard Galinier , Steven van Beurden , Elsa Amilhat , Jeannette Castric , Guy Schoehn
Virus Res, 2012, 166 (1-2), pp.1-12. ⟨10.1016/j.virusres.2012.02.020⟩
Journal articles halsde-00700373v1

Expression patterns of Abd-A/Lox4 in a monogenean parasite with alternative developmental paths.

Mathieu Badets , Guillaume Mitta , Richard Galinier , Olivier Verneau
Molecular and Biochemical Parasitology, 2010, 173 (2), pp.154-157. ⟨10.1016/j.molbiopara.2010.05.011⟩
Journal articles halsde-00504771v1

High phenotypic frequencies of complete albinism in wild populations of Biomphalaria pfeifferi (Gastropoda: Pulmonata)

Gabriel Mouahid , Rodrigue Mintsa Nguema , M.A. Idris , M.A. Shaban , S. Al Yafee
Malacologia, 2010, 53 (1), pp.161-166
Journal articles halsde-00608656v1

Halocyntin and papillosin, two new antimicrobial peptides isolated from hemocytes of the solitary tunicate, Halocynthia papillosa

Richard Galinier , E. Roger , P.E. Sautière , A. Aumelas , B. Banaigs
Journal of Peptide Science, 2009, 15 (1), pp.48-55. ⟨10.1002/psc.1101⟩
Journal articles halsde-00371895v1
Image document

Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages

S. Beltran , Richard Galinier , Jean-François Allienne , Jérôme Boissier
Memórias do Instituto Oswaldo Cruz, 2008, 103 (5), pp.501-503
Journal articles halsde-00344405v1

Expression analysis of highly polymorphic mucin proteins (Sm PoMuc) from the parasite Schistosoma mansoni

E. Roger , Benjamin Gourbal , Christoph Grunau , R.J. Pierce , Richard Galinier
Molecular and Biochemical Parasitology, 2008, 157, pp.217-227. ⟨10.1016/j.molbiopara.2007.11.015⟩
Journal articles halsde-00286479v1
Image document

Controlled chaos of polymorphic mucins in a metazoan parasite (Schistosoma mansoni) interacting with its invertebrate host (Biomphalaria glabrata).

Emmanuel Roger , Christoph Grunau , Raymond J. Pierce , Hirohisa Hirai , Benjamin Gourbal
PLoS Neglected Tropical Diseases, 2008, 2 (11), pp.e330. ⟨10.1371/journal.pntd.0000330⟩
Journal articles halsde-00344615v1

Identification and expression of gene transcripts generated during an anti-parasitic response in Biomphalaria glabrata.

François Guillou , Guillaume Mitta , Richard Galinier , Christine Coustau
Developmental and Comparative Immunology, 2007, 31 (7), pp.657-71. ⟨10.1016/j.dci.2006.10.001⟩
Journal articles hal-00170685v1

Gene discovery and expression analysis of immune-relevant genes from Biomphalaria glabrata hemocytes.

G. Mitta , Richard Galinier , P. Tisseyre , Jean-François Allienne , Y. Girerd-Chambaz
Developmental and Comparative Immunology, 2005, 29 (5), pp.393-407. ⟨10.1016/j.dci.2004.10.002⟩
Journal articles hal-00104494v1

Characterisation of proteins differentially present in the plasma of Biomphalaria glabrata susceptible or resistant to Echinostoma caproni.

D. Vergote , A. Bouchut , P. E. Sautière , E. Roger , Richard Galinier
International Journal for Parasitology, 2005, 35 (2), pp.215-24. ⟨10.1016/j.ijpara.2004.11.006⟩
Journal articles hal-00104691v1

Adenovirus protein involved in virus internalization recruits ubiquitin-protein ligases.

Richard Galinier , Evelyne Gout , Hugues Lortat-Jacob , Jonathan Wood , Jadwiga Chroboczek
Biochemistry, 2002, 41 (48), pp.14299-305
Journal articles hal-01061430v1

Cracking the code of Pacific oyster mortality syndrom

Guillaume Mitta , Julien de Lorgeril , Aude Lucasson , Bruno Petton , Eve Toulza
Aqua 2018 14th International Symposium on Aquaculture Nutrition, Aug 2018, Montpellier, France
Conference papers hal-03965691v1

A systems biology approach linking genetic, epigenetic, cytoplasmic and holobiont inheritance to understand rapid adaptation of parasitic flatworms (Schistosoma sp.)

Céline Cosseau , Eve Toulza , Richard Galinier , Benjamin Gourbal , Christoph Grunau
66th Annual Meeting of the American Society of Tropical Medicine and Hygiene, Nov 2017, Baltimore, United States. pp.183
Conference papers hal-03132820v1

An integrative approach to decipher the summer mortality syndrome affecting Pacific oysters

Julien de Lorgeril , Aude Lucasson , Bruno Petton , Eve Toulza , Philippe Haffner
Immuninv 2017, Jun 2017, Lyon, France
Conference papers hal-03966171v1

An integrative approach to decipher the mortality syndrome affecting juvenile Pacific oysters

Julien de Lorgeril , Aude Lucasson , Bruno Petton , Eve R Toulza , Philippe Haffner
International Conference on Holobionts, Apr 2017, Paris, France
Conference papers hal-03965715v1

Proportions élevées d'albinisme en population naturelle chez Biomphalaria pfeifferi : implication dans la transmission de Schistosoma mansoni en Oman

R. Mintsa Nguema , Juliette Langand , Richard Galinier , H. Moné , G. Mouahid
30e Petit Pois Déridé, Aug 2008, Rennes, France
Conference papers halsde-00330465v1