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Stéphane GUYOT

Researcher identifiers
  • IdHAL stephane-guyot
  • ResearcherId : A-3482-2012
  • ORCID 0000-0002-2316-234X
  • Google Scholar :
  • IdRef : 124704298
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**Dr Stéphane GUYOT** **Lecturer** UMR PAM, team PMB – AgroSup Dijon – Université de Bourgogne Franche-Comté 1, Esplande Erasme 21000 Dijon, France +33 (0)3 80 77 23 87 <> or <> **1) Situation** Nationality: French and Swiss Date of birth: 23rd August 1977 Marital status: married, 4 children **2) Education and Career** **Education** **2002-2007 Ph D**, ENSBANA, University of Burgundy (Dijon, France) **2001-2002 Master 2 Research of Food Sciences**, University of Burgundy **1999-2000 Master 1 of Cellular and Physiological Biology**, University of Burgundy **1998-1999 Bachelor of Science in Cellular and Physiological Biology**, University of Burgundy **1995-1997 Technical Degree in Biological and Biochemical Analysis**, University of Burgundy **Career** **2008-présent Lecturer: Microbiological and Food Processes**, AgroSup Dijon (France) **2007-2008 Teacher-Researcher (temporary, 50%)**, ENSBANA, University of Burgundy **2005-2006 Teacher-Researcher (temporary, 50%)**, ENSBANA, University of Burgundy **2003-2005 High-education teacher**, ENSBANA, University of Burgundy Main field of research: Study of microorganism (pathogenic bacteria, yeasts) stress response to improve food processes (as destruction of undesired microorganisms). Study of the impact of different parameter (as magnitude, kinetics and holding time) inherent to an environmental perturbation (as heat treatments, drying) · Use of wild type and mutant strains (KO strains) · Impact on cell components (membranes, proteins, sphingolipids, stress granules): flow cytometry, fluorescent microscopy (confocal, two-photon), Fourier-Transform Infrared Spectroscopy … Responsabilities (most significant) / scientific activities 02/2018 Coordinator of the national workshop “Fight against pathogenic and alteration flora” (Dijon, France) 01/2016-present One of the three heads of the International Research Master “Microbiology and Physicochemitry for Food and Wine Processes (MP²)” 01/2017-10/2018 Co deputy then Deputy Head of the “Procédés Microbiologiques et Biotechnologiques” research team (UMR PAM) 04/2016-present Co-responsible of a transversal research area (UMR PAM) 2012-2015 Head of the 2 years preparatory classes (integrated in AgroSup Dijon) 2012-2013 Young doctor expert for AERES (evaluation of doctoral schools in Life Sciences at Paris 6, France) 2008-2017 Reviewer for international peer-reviewed scientific journals: 13 papers 2014-present Examiner in 3 thesis juries **2) Scientific production** ***Web of Sciences*** ***Research Gate*** ***Google Scholar*** ***Number of citations*** 270 306 400 ***H*** 10 11 13 ***H/n (n = 14)*** 0.71 0.78 0.92 - 30 articles published in peer-reviewed international scientific journals - International congresses : 7 oral presentations (including 1 invited) + 11 written presentations - National congresses : 7 oral presentations + 12 written presentations - Regional congresses : 1 oral presentation + 6 written presentations - Local seminar : 2 oral presentations abroad + 1 oral presentation in France **3) Co-supervision** - Thesis: 2 thesis co-supervised between 2013-2016 (ANR EcoSec 33% and CIFRE: 50%), 1 thesis co-supervised since October 2016 (MESR grant: 50%) - Postdoctoral training: 2 postdoctoral researchers supervised (100% each, 2015 and 2016) - Master 2 Research: 13 students co-supervised (1x100% and 12 x 50%)


The Yin-Yang of the Green Fluorescent Protein: Impact on Saccharomyces cerevisiae stress resistance

Mélanie Ragon , Lucie Bertheau , Jennifer Dumont , Tiffany Bellanger , Marie Grosselin
Journal of Photochemistry and Photobiology B: Biology, 2023, 238, pp.112603. ⟨10.1016/j.jphotobiol.2022.112603⟩
Journal articles hal-03902943v1
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Experimental evolution forcing Oenococcus oeni acid tolerance highlights critical role of the citrate locus

Frédérique Julliat , Camille Eicher , Nezha Tourti , Philippe Glaser , Nicolas Cabanel
Research in Microbiology, 2023, 174 (5), pp.104048. ⟨10.1016/j.resmic.2023.104048⟩
Journal articles hal-04034609v1
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The potential of microorganisms as biomonitoring and bioremediation tools for mercury-contaminated soils

Lorraine Meyer , Stéphane Guyot , Michel Chalot , Nicolas Capelli
Ecotoxicology and Environmental Safety, 2023, 262, pp.115185. ⟨10.1016/j.ecoenv.2023.115185⟩
Journal articles hal-04152835v1
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Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope

Fiona Zoz , Stéphane Guyot , Cosette Grandvalet , Mélanie Ragon , Eric Lesniewska
Foods, 2021, 10 (9), pp.1-15. ⟨10.3390/foods10092002⟩
Journal articles hal-03327279v1
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Cardiolipin content controls mitochondrial coupling and energetic efficiency in muscle

Alexandre Prola , Jordan Blondelle , Aymeline Vandestienne , Jérôme Piquereau , Raphaël Denis
Science Advances , 2021, 7 (1), pp.eabd6322. ⟨10.1126/sciadv.abd6322⟩
Journal articles hal-03105418v1
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Increased xerotolerance of Saccharomyces cerevisiae during an osmotic pressure ramp over several generations

Stéphane Guyot , Laurence Pottier , Lucie Bertheau , Jennifer Dumont , Eminence Dorelle Hondjuila Miokono
Microbial Biotechnology, 2021, 14 (4), pp.1445-1461. ⟨10.1111/1751-7915.13789⟩
Journal articles hal-03174738v1
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Estimation of Microbial Viability Using Flow Cytometry

Hazel Davey , Stéphane Guyot
Current Protocols in Cytometry, 2020, 93 (1), pp.1-13. ⟨10.1002/cpcy.72⟩
Journal articles hal-02544120v1
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Highlighting Protective Effect of Encapsulation on Yeast Cell Response to Dehydration Using Synchrotron Infrared Microspectroscopy at the Single-Cell Level

Thanh Dat Nguyen , Stéphane Guyot , Caroline Pénicaud , Stéphanie Passot , Christophe Sandt
Frontiers in Microbiology, 2020, 11 (6), pp.1887. ⟨10.3389/fmicb.2020.01887⟩
Journal articles hal-02939385v1

Automatic Counting of Intra-Cellular Ribonucleo-Protein Aggregates in Saccharomyces cerevisiae Using a Textural Approach

Ambroise Marin , Emmanuel Denimal , Lucie Bertheau , Stéphane Guyot , Ludovic Journaux
Microscopy and Microanalysis, 2019, 25 (1), pp.164-179. ⟨10.1017/S1431927619000084⟩
Journal articles hal-02171876v1
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Cellular Injuries in Cronobacter sakazakii CIP 103183T and Salmonella enterica Exposed to Drying and Subsequent Heat Treatment in Milk Powder

Emilie Lang , Stéphane Guyot , Caroline Peltier , Pablo Alvarez-Martin , Jean-Marie Perrier-Cornet
Frontiers in Microbiology, 2018, 9, pp.475. ⟨10.3389/fmicb.2018.00475⟩
Journal articles hal-01907589v1

Caco-2 Invasion by Cronobacter sakazakii and Salmonella enterica Exposed to Drying and Heat Treatments in Dried State in Milk Powder

Émilie Lang , Stéphane Guyot , Pablo Alvarez-Martin , Jean-Marie Perrier-Cornet , Patrick Gervais
Frontiers in Microbiology, 2017, 8, ⟨10.3389/fmicb.2017.01893⟩
Journal articles hal-01618841v1

Comparison of the effects of major fatty acids present in the Mediterranean diet (oleic acid, docosahexaenoic acid) and in hydrogenated oils (elaidic acid) on 7-ketocholesterol-induced oxiapoptophagy in microglial BV-2 cells

Meryam Debbabi , Amira Zarrouk , Maryem Bezine , Wiem Meddeb , Thomas Nury
Chemistry and Physics of Lipids, 2017, 207 (Pt B), pp.151-170. ⟨10.1016/j.chemphyslip.2017.04.002⟩
Journal articles hal-01912331v1

Modeling the heat inactivation of foodborne pathogens in milk powder : High relevance of the substrate water activity

Emilie Lang , Layla Chemlal , Paul Molin , Stéphane Guyot , Pablo Alvarez-Martin
Food Research International, 2017, 99 (1), pp.577-585. ⟨10.1016/j.foodres.2017.06.028⟩
Journal articles hal-01558447v1

Understanding the responses of Saccharomyces cerevisiae yeast strain during dehydration processes using synchrotron infrared spectroscopy

T. Nguyen , Stéphane Guyot , C. C. Penicaud , S. Passot , C. Sandt
Analyst, 2017, 142 (19), pp.3620 - 3628. ⟨10.1039/c7an00257b⟩
Journal articles hal-01616052v1

Drying parameters greatly affect the destruction of Cronobacter sakazakii and Salmonella Typhimurium in standard buffer and milk

Emilie Lang , Cyril Laconelli , Fiona Zoz , Stéphane Guyot , Pablo Alvarez-Martin
Food Microbiology, 2017, 62, pp.82 - 91. ⟨10.1016/⟩
Journal articles hal-01509615v1

Listeria monocytogenes ability to survive desiccation: Influence of serotype, origin, virulence, and genotype

Fiona Zoz , Cosette Grandvalet , Emilie Lang , Cyril Laconelli , Patrick Gervais
International Journal of Food Microbiology, 2017, 248, pp.82 - 89. ⟨10.1016/j.ijfoodmicro.2017.02.010⟩
Journal articles hal-01509875v1

Automatic Biological Cell Counting Using a Modified Gradient Hough Transform

Emmanuel Denimal , Ambroise Marin , Stéphane Guyot , Ludovic Journaux , Paul Molin
Microscopy and Microanalysis, 2017, 23 (01), pp.11 - 21. ⟨10.1017/S1431927616012617⟩
Journal articles hal-01509941v1

Design of new sensitive α,β-unsaturated carbonyl 1,8-naphtalimide fluorescent probes for thiol bioimaging

Nicolas Sok , Maria Nikolantonaki , Stéphane Guyot , Thanh Dat Nguyen , Anne-Sophie Viaux
Sensors and Actuators B: Chemical, 2017, 242, pp.865 - 871. ⟨10.1016/j.snb.2016.09.171⟩
Journal articles hal-01509840v1

Influence of the air humidity on the drying of a liquid droplet on a solid plate and on bacterial destruction

Onrawee Laguerre , L. Lecoq , F. Zoz , Stéphane Guyot , Laurent Beney
Journal of Food Engineering, 2017, 212, pp.76-86. ⟨10.1016/j.jfoodeng.2017.05.014⟩
Journal articles hal-01558465v1

Innovative High Gas Pressure Microscopy Chamber Designed for Biological Cell Observation

Mélanie Ragon , Hue Nguyen Thi Minh , Stéphane Guyot , Pauline Loison , Gaëtan Burgaud
Microscopy and Microanalysis, 2016, 22 (01), pp.63 - 70. ⟨10.1017/S1431927615015639⟩
Journal articles hal-01485208v1

Recovery Estimation of Dried Foodborne Pathogens Is Directly Related to Rehydration Kinetics

Émilie Lang , Fiona Zoz , Cyril Laconelli , Stéphane Guyot , Pablo Alvarez-Martin
PLoS ONE, 2016, 11 (8), ⟨10.1371/journal.pone.0160844⟩
Journal articles hal-01466823v1

Control of Relative Air Humidity as a Potential Means to Improve Hygiene on Surfaces: A Preliminary Approach with Listeria monocytogenes

Fiona Zoz , Cyril Laconelli , Émilie Lang , Stéphane Guyot , Cosette Grandvalet
PLoS ONE, 2016, 11 (2), ⟨10.1371/journal.pone.0148418⟩
Journal articles hal-01466198v1

Protection of yeast cells in micro-organized shells of natural polyelectrolytes during drying process

Thanh Dat Nguyen , Stéphane Guyot , Christophe Sandt , Caroline Pénicaud , Stéphanie Passot
STP Pharma Pratiques, 2016, 26 (2), pp.133
Journal articles hal-01531646v1

An Off-On-Off Fluorescent Sensor for pH Windows Based on the 13aneN4-Zn 2+ System

Valeria Amendola , Greta Bergamaschi , Giacomo Dacarro , Franck Denat , Frederic Boschetti
European Journal of Inorganic Chemistry, 2016, 2016 (32), pp.5106 - 5113. ⟨10.1002/ejic.201600749⟩
Journal articles hal-01492871v1

A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting

Ambroise Marin , Emmanuel Denimal , Stéphane Guyot , Ludovic Journaux , Paul Molin
Microscopy and Microanalysis, 2015, 21 (01), pp.239-248. ⟨10.1017/S1431927614013671⟩
Journal articles hal-01114543v1

Reliable Detection and Smart Deletion of Malassez Counting Chamber Grid in Microscopic White Light Images for Microbiological Applications

Emmanuel Denimal , Ambroise Marin , Ludovic Journaux , Stéphane Guyot , Paul Molin
Microscopy and Microanalysis, 2015, pp.1-7. ⟨10.1017/S1431927615000665⟩
Journal articles hal-01164294v1

Surviving the heat: heterogeneity of response in S accharomyces cerevisiae provides insight into thermal damage to the membrane

Stéphane Guyot , Patrick Gervais , Michael Young , Pascale Winckler , Jennifer Dumont
Environmental Microbiology, 2015, 17 (8), pp.2982-2992. ⟨10.1111/1462-2920.12866⟩
Journal articles hal-02290604v1

Protection of living yeast cells by micro-organized shells of natural polyelectrolytes

Thanh Dat Nguyen , Stéphane Guyot , Jeannine Lherminier , Yves Waché , Rémi Saurel
Process Biochemistry, 2015, 50 (10), pp.1528-1536. ⟨10.1016/j.procbio.2015.06.003⟩
Journal articles hal-02290612v1

Hydrodynamic characterization of a new small-scale reactor mixed by a magnetic bar

Charline Allonneau , Eric Olmos , Stéphane Guyot , Eric Ferret , Patrick Gervais
Biochemical Engineering Journal, 2015, 96, pp.29-37. ⟨10.1016/j.bej.2014.12.005⟩
Journal articles hal-01267213v1
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HACD1 , a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth

Jordan Blondelle , Yusuke Ohno , Vincent Gache , Stéphane Guyot , Sébastien Storck
Journal of molecular cell biology, 2015, 7 (5), pp.429-440. ⟨10.1093/jmcb/mjv049⟩
Journal articles hal-02290631v1
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Extremely rapid acclimation of Escherichia coli to high temperature over a few generations of a fed-batch culture during slow warming

Stéphane Guyot , Laurence Pottier , Alain Hartmann , Mélanie Ragon , Julia Hauck Tiburski
MicrobiologyOpen, 2014, 3 (1), pp.52-63. ⟨10.1002/mbo3.146⟩
Journal articles hal-02290963v1

Water Distribution in Bacterial Spores: A Key Factor in Heat Resistance

Julia Hauck Tiburski , Amauri Rosenthal , Stéphane Guyot , Jean-Marie Perrier-Cornet , Patrick Gervais
Food Biophysics, 2014, 9 (1), pp.10-19. ⟨10.1007/s11483-013-9312-5⟩
Journal articles hal-02290639v1

Exploration of Lipid Metabolism in Relation with Plasma Membrane Properties of Duchenne Muscular Dystrophy Cells: Influence of L-Carnitine

Françoise Le Borgne , Stéphane Guyot , Morgan Logerot , Laurent Beney , Patrick Gervais
PLoS ONE, 2012, 7 (11), pp.e49346. ⟨10.1371/journal.pone.0049346⟩
Journal articles hal-02291000v1

Physiological responses of Escherichia coli exposed to different heat-stress kinetics

Stéphane Guyot , Laurence Pottier , Eric Ferret , Laurent Gal , Patrick Gervais
Archives of Microbiology, 2010, 192 (8), pp. 651 - 661. ⟨10.1007/s00203-010-0597-1⟩
Journal articles hal-01789685v1

Phospholipidosis and down-regulation of the PI3-K/PDK-1/Akt signalling pathway are vitamin E inhibitable events associated with 7-ketocholesterol-induced apoptosis

Anne Vejux , Stéphane Guyot , Thomas Montange , Jean-Marc Riedinger , Edmond Kahn
The Journal of Nutritional Biochemistry, 2009, 20 (1), pp.45-61. ⟨10.1016/j.jnutbio.2007.12.001⟩
Journal articles hal-02291044v1

Erratum to: Effect of the osmotic conditions during sporulation on the subsequent resistance of bacterial spores

Hue Nguyen Thi Minh , Stéphane Guyot , Jean-Marie Perrier-Cornet , Patrick Gervais
Applied Microbiology and Biotechnology, 2008, 80 (1), pp.107-114. ⟨10.1007/s00253-008-1519-x⟩
Journal articles hal-02291056v1

Yeast cell inactivation related to local heating induced by low-intensity electric fields with long-duration pulses

Stéphane Guyot , Eric Ferret , Jean-Baptiste Boehm , Patrick Gervais
International Journal of Food Microbiology, 2007, 113 (2), pp.180-188. ⟨10.1016/j.ijfoodmicro.2006.06.036⟩
Journal articles hal-02291104v1

Yeast Survival during Thermal and Osmotic Shocks Is Related to Membrane Phase Change

Stéphane Guyot , Eric Ferret , Patrick Gervais
Journal of Agricultural and Food Chemistry, 2006, 54 (22), pp.8450-8455. ⟨10.1021/jf0620158⟩
Journal articles hal-02291112v1

Responses of Saccharomyces cerevisiae to thermal stress

Stéphane Guyot , Eric Ferret , Patrick Gervais
Biotechnology and Bioengineering, 2005, 92 (4), pp.403-409. ⟨10.1002/bit.20600⟩
Journal articles hal-02295180v1