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Valérie LABAS

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Après un parcours universitaire en physiologie animale, microbiologie et biochimie, j’ai obtenu un DEA « Structure et système biologique intégré » en1999 à l'Université d'Orsay (Paris XI). J’ai intégré le CNRS en 1999 à l'ESPCI Paris Tech en tant qu’ingénieur d’Etudes en Protéomique. Après 6 ans, en 2005, j’ai engagé une mobilité pour intégrer l’UMR PRC du Centre de recherche INRAE Val de Loire. Par voie de concours interne en 2007, j’ai intégré l’INRA en tant qu’Ingénieur de Recherche et évolué à différents postes de responsabilité au sein des plates-formes (PPAF >PAIB2>CIRE>PIXANIM). Actuellement, je suis directrice adjointe de la plate-forme PIXANIM (certifiée ISO 9001 v 2015, labellisée IBiSa). Mon domaine d'expertise est la spectrométrie de masse appliquée à la protéomique et plus récemment, depuis 2015, à la lipidomique. Mon activité porte sur la spectrométrie de masse en tandem à haute résolution couplée à la nano-chromatographie liquide (spectromètre de masse LTQ VelosPro Orbitrap, Thermofisher) et la spectrométrie de masse MALDI-TOF (RapifleX, Bruker daltonics). En tant que responsable scientifique dans mon domaine d’expertise, je participe à la réalisation de ≈ 60 projets/an d’envergure régionale, nationale européenne et internationale dans le cadre de la recherche (projets Région Centre, ANR et Européens) et de l’innovation (2 projets de pré-maturation, projet de création d’une start-up). Pour mener à bien ces projets, j’emploie différentes stratégies analytiques : 1\) phénotypage moléculaire par MALDI-TOF à partir de l’analyse directe protéomique/lipidomique de fluides biologiques ou de cellules entières eucaryotes supérieures en association avec du « machine learning » pour la création de tests diagnostics en intelligence artificielle ; 2\) le phénotypage tissulaire ou imagerie moléculaire par MALDI-TOF à partir de coupes de tissus ; 3\) les approches Top-down pour l’identification des molécules endogènes intactes et entières (analyse structurale des protéines ou lipides) et ; 4\) les approches classiques de protéomique qualitative et quantitative de type label-free par bottom-up.
After a university training in animal physiology, microbiology and biochemistry, I obtained a DEA (post-graduate diploma) in "Structure and integrated biological system" in 1999 at the University of Orsay (Paris XI). I joined the CNRS in 1999 at the ESPCI Paris Tech as an engineer in Proteomics. After 6 years, in 2005, I moved to the UMR PRC of the INRAE Val de Loire Research Centre. In 2007, I joined INRAE as a Research Engineer and held various positions of responsibility within the platforms (PPAF >PAIB2>CIRE>PIXANIM). Currently, I am deputy director of the PIXANIM platform (ISO 9001 v 2015 certified, IBiSa labelled). My field of expertise is mass spectrometry applied to proteomics and more recently, since 2015, to lipidomics. My activity focuses on high resolution tandem mass spectrometry coupled to liquid nano-chromatography (LTQ VelosPro Orbitrap mass spectrometer, Thermofisher) and MALDI-TOF mass spectrometry (RapifleX, Bruker daltonics). I participate in the realization of ≈ 60 projects/year of regional, national, European and international scope in the framework of research (Région Centre, ANR and European projects) and innovation (2 pre-maturation projects, start-up project). To carry out these projects, I use different analytical strategies: 1\) molecular phenotyping by MALDI-TOF from direct proteomic/lipidomic analysis of biological fluids or higher eukaryotic whole cells in combination with machine learning for the creation of diagnostic tests in artificial intelligence; 2\) Tissue phenotyping or molecular imaging by MALDI-TOF from tissue sections; 3\) Top-down approaches for the identification of intact and whole endogenous molecules (structural analysis of proteins or lipids) and 4\) classical bottom-up label-free qualitative and quantitative proteomics approaches.

Publications

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Extracellular Vesicles Contribute to the Difference in Lipid Composition between Ovarian Follicles of Different Size Revealed by Mass Spectrometry Imaging

Emilie Maugrion , Ekaterina N Shedova , Rustem Uzbekov , Ana-Paula Teixeira-Gomes , Valérie Labas
Metabolites, 2023, 13 (9), pp.1001. ⟨10.3390/metabo13091001⟩
Journal articles hal-04214124v1

Metabolic exchanges between the oocyte and its environment: focus on lipids

Svetlana Uzbekova , Priscila Silvana Bertevello , Rozenn Dalbies-Tran , Sebastien Elis , Valerie Labas
Reproduction, Fertility and Development, 2022, 34 (2), pp.1-26. ⟨10.1071/RD21249⟩
Journal articles hal-03457344v1
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The proteomic analysis of bovine embryos developed in vivo or in vitro reveals the contribution of the maternal environment to early embryo

Charles Banliat , Coline Mahé , R Lavigne , Emmanuelle Com , Charles Pineau
BMC Genomics, 2022, 23 (1), pp.839. ⟨10.1186/s12864-022-09076-5⟩
Journal articles hal-03923178v1
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Dynamic changes in the proteome of early bovine embryos developed in vivo

Charles Banliat , Coline Mahe , Régis Lavigne , Emmanuelle Com , Charles Pineau
Frontiers in Cell and Developmental Biology, 2022, 10, pp.1-16. ⟨10.3389/fcell.2022.863700⟩
Journal articles hal-03658017v1
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Multifaceted roles of the egg perivitelline layer in avian reproduction: Functional insights from the proteomes of chicken egg inner and outer sublayers

Mégane Brégeon , Daniel Tomas , Benoît Bernay , Céline Zatylny-Gaudin , Sonia Georgeault
Journal of Proteomics, 2022, 258, pp.104489. ⟨10.1016/j.jprot.2022.104489⟩
Journal articles hal-03541518v1

Protein palmitoylation in bovine ovarian follicle

Svetlana Uzbekova , Ana-Paula Teixeira-Gomes , Aurélie Marestaing , Peggy Jarrier-Gaillard , Pascal Papillier
International Journal of Molecular Sciences, 2021, 22 (21), pp.1-27. ⟨10.3390/ijms222111757⟩
Journal articles hal-03457402v1
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The defibrillation conundrum: new insights into the mechanisms of shock-related myocardial injury sustained from a life-saving therapy

Nicolas Clementy , Alexandre Bodin , Arnaud Bisson , Ana-Paula Teixeira-Gomes , Sebastien Roger
International Journal of Molecular Sciences, 2021, 22 (9), pp.1-17. ⟨10.3390/ijms22095003⟩
Journal articles hal-03741929v1
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Pluripotent stem cell derived dopaminergic subpopulations model the selective neuron degeneration in Parkinson’s disease

Tony Oosterveen , Pedro Garção , Emma Moles-Garcia , Clement Soleilhavoup , Marco Travaglio
Stem Cell Reports, 2021, 16 (11), pp.2718 - 2735. ⟨10.1016/j.stemcr.2021.09.014⟩
Journal articles hal-03681035v1
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Genetic determinants of seed protein plasticity in response to the environment in Medicago truncatula

Kevin Cartelier , Delphine Aimé , Joseph Ly Vu , Lucie Combes‐soia , Valérie Labas
Plant Journal, 2021, pp.Early Access. ⟨10.1111/TPJ.15236⟩
Journal articles hal-03177967v1
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Three‐dimensional structures of avian beta‐microseminoproteins: insight from the chicken egg‐specific beta‐microseminoprotein 3 paralog

Franck Coste , Thierry Moreau , Valérie Labas , Magali Chessé , Mégane Brégeon
FEBS Open Bio, 2021, 11 (6), pp.1739-1756. ⟨10.1002/2211-5463.13166⟩
Journal articles hal-03216446v1
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Use of MALDI-TOF mass spectrometry to explore the peptidome and proteome of in-vitro produced bovine embryos pre-exposed to oviduct fluid

Charles Banliat , Valérie Labas , Daniel Tomas , Ana-Paula Teixeira-Gomes , Benoît Guyonnet
Reproductive biology, 2021, 21 (4), pp.1-5. ⟨10.1016/j.repbio.2021.100545⟩
Journal articles hal-03511989v1
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Proteomic Changes Associated With Sperm Fertilizing Ability in Meat-Type Roosters

Anais Vitorino Carvalho , Laura Soler , Aurore Thélie , Isabelle Grasseau , Luiz Cordeiro
Frontiers in Cell and Developmental Biology, 2021, 9, 15 p. ⟨10.3389/fcell.2021.655866⟩
Journal articles hal-03213124v1
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Avian uterine fluid proteome: exosomes and biological processes potentially involved in sperm survival

Cindy Riou , Aurélien Brionne , Luiz-Augusto Cordeiro , Grégoire Harichaux , Audrey Gargaros-Ratajczak
Molecular Reproduction and Development, 2020, acceptée (4), Acceptée. ⟨10.1002/mrd.23333⟩
Journal articles hal-01605894v1

Beta-nerve growth factor stimulates spontaneous electrical activity of in vitro embryonic mouse GnRH neurons through a P75 mediated-mechanism

Caroline Pinet-Charvet , Renaud Fleurot , Flavie Derouin-Tochon , Simon de Graaf , Xavier Druart
Scientific Reports, 2020, 10 (1), 14 p. ⟨10.1038/s41598-020-67665-4⟩
Journal articles hal-03094626v1
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Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct

Charles Banliat , Guillaume Tsikis , Valérie Labas , Ana-Paula Teixeira-Gomes , Emmanuelle Com
International Journal of Molecular Sciences, 2020, 21 (2), 17 p. ⟨10.3390/ijms21020466⟩
Journal articles hal-02448383v1
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Progesterone induces sperm release from oviductal epithelial cells by modifying sperm proteomics, lipidomics and membrane fluidity

Marina Ramal-Sanchez , Nicola Bernabo , Guillaume Tsikis , Marie-Claire Blache , Valérie Labas
Molecular and Cellular Endocrinology, 2020, 504, pp.1-10. ⟨10.1016/j.mce.2020.110723⟩
Journal articles hal-03151338v1
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Intact cell MALDI-TOF mass spectrometry, a promising proteomic profiling method in farm animal clinical and reproduction research

Laura Soler , Svetlana Uzbekova , Elisabeth Blesbois , Xavier Druart , Valérie Labas
Theriogenology, 2020, 150, pp.113-121. ⟨10.1016/j.theriogenology.2020.02.037⟩
Journal articles hal-02860780v1
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Protein Cargo of Extracellular Vesicles From Bovine Follicular Fluid and Analysis of Their Origin From Different Ovarian Cells

Svetlana Uzbekova , Carmen Alminana , Valérie Labas , Ana-Paula Teixeira-Gomes , Lucie Combes-Soia
Frontiers in Veterinary Science, 2020, 7, 20 p. ⟨10.3389/fvets.2020.584948⟩
Journal articles hal-02995254v1
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Intrinsic alterations in peripheral neutrophils from cystic fibrosis newborn piglets

Deborah Brea , Laura Soler , Isabelle Fleurot , Sandrine Melo , Claire Chevaleyre
Journal of Cystic Fibrosis, 2020, 19 (5), pp.830-836. ⟨10.1016/j.jcf.2020.02.016⟩
Journal articles hal-03012482v1

Acute pathophysiological myocardial changes following intra-cardiac electrical shocks using a proteomic approach in a sheep model

Alexandre Bodin , Valérie Labas , Arnaud Bisson , Ana-Paula Teixeira-Gomes , Hélène Blasco
Scientific Reports, 2020, 10 (1), 12 p. ⟨10.1038/s41598-020-77346-x⟩
Journal articles hal-03151051v1
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Structure, function, and evolution of Gga -AvBD11, the archetype of the structural avian-double-β-defensin family

Nicolas Guyot , Sascha Trapp , Herve Meudal , Sophie Iochmann , Anne Silvestre
Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (1), pp.337-345. ⟨10.1073/pnas.1912941117⟩
Journal articles hal-02522250v1
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MALDI-TOF Mass Spectrometry Revealed Significant Lipid Variations in Follicular Fluid and Somatic Follicular Cells but Not in Enclosed Oocytes between the Large Dominant and Small Subordinate Follicles in Bovine Ovary

Priscila Silvana Bertevello , Ana-Paula Teixeira-Gomes , Valérie Labas , Luiz Cordeiro , Marie-Claire Blache
International Journal of Molecular Sciences, 2020, 21 (18), 30 p. ⟨10.3390/ijms21186661⟩
Journal articles hal-02959768v1
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Oviduct Fluid Extracellular Vesicles Change the Phospholipid Composition of Bovine Embryos Developed In Vitro

Charles Banliat , Daniel Le Bourhis , Ophélie Bernardi , Daniel Tomas , Valérie Labas
International Journal of Molecular Sciences, 2020, 21 (15), pp.1-13. ⟨10.3390/ijms21155326⟩
Journal articles hal-03151188v1
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Graphene oxide: A glimmer of hope for Assisted Reproductive Technology

Marina Ramal-Sanchez , Luca Valbonetti , Guillaume Tsikis , Florine Dubuisson , Marie-Claire Blache
Carbon, 2019, 150, pp.518 - 530. ⟨10.1016/j.carbon.2019.05.055⟩
Journal articles hal-02619027v1

Intraoviductal concentrations of steroid hormones during in vitro culture changed phospholipid profiles and cryotolerance of bovine embryos

Charles Banliat , Florine Dubuisson , Emilie Corbin , Julie Beurois , Daniel Tomas
Molecular Reproduction and Development, 2019, 86 (6), pp.661 - 672. ⟨10.1002/mrd.23144⟩
Journal articles hal-02628367v1
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Polymer “ruthenium-cyclopentadienyl” conjugates - New emerging anti-cancer drugs

Tiago Moreira , Rita Francisco , Elisabeta Comsa , Sophie S. Duban-Deweer , Valérie Labas
European Journal of Medicinal Chemistry, 2019, 168, pp.373-384. ⟨10.1016/j.ejmech.2019.02.061⟩
Journal articles hal-02325931v1
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The unique features of proteins depicting the chicken amniotic fluid

Mylène da Silva , Clara Dombre , Aurélien Brionne , Philippe Monget , Magali Chessé
Molecular and Cellular Proteomics, 2019, 18 (3), pp.S174-S190. ⟨10.1074/mcp.RA117.000459⟩
Journal articles hal-02626106v1
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Guinea fowl eggshell quantitative proteomics yield new findings related to its unique structural characteristics and superior mechanical properties

Nathalie Le Roy , Lucie Combes-Soia , Aurélien Brionne , Valérie Labas , Alejandro B. Rodriguez-Navarro
Journal of Proteomics, 2019, 209, ⟨10.1016/j.jprot.2019.103511⟩
Journal articles hal-02623244v1

Proteomic analysis of uterine fluid of fertile and subfertile hens before and after insemination

Cindy Riou , Aurélien Brionne , Luiz Cordeiro , Grégoire Harichaux , Audrey Gargaros
Reproduction -Cambridge- Supplement-, 2019, 158 (4), pp.335-356. ⟨10.1530/REP-19-0079⟩
Journal articles hal-02622969v1
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Digestion dynamics in broilers fed rapeseed meal

Emilie Recoules , Michel Lessire , Valérie Labas , Michel Jacques M.J. Duclos , Lucie Combes-Soia
Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-019-38725-1⟩
Journal articles hal-02628279v1
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Water stress combined with sulfur deficiency in pea affects yield components but mitigates the effect of deficiency on seed globulin composition

Charlotte Henriet , Delphine Aime , Morgane Terezol , Anderson Kilandamoko , Nadia Rossin
Journal of Experimental Botany, 2019, 70 (16), pp.4287 - 4303. ⟨10.1093/jxb/erz114⟩
Journal articles hal-02627230v1
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Effects of a n-3 polyunsaturated fatty acid-enriched diet on embryo production in dairy cows

Sandrine Freret , Mouhamad Oseikria , Daniel Le Bourhis , Alice Desmarchais , Eric Briant
Reproduction -Cambridge- Supplement-, 2019, 158 (1), pp.71-83. ⟨10.1530/REP-18-0644⟩
Journal articles hal-02623225v1
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Stage-dependent changes in oviductal phospholipid profiles throughout the estrous cycle in cattle

Charles Banliat , Daniel Tomas , Ana-Paula Teixeira-Gomes , Svetlana Uzbekova , Benoît Guyonnet
Theriogenology, 2019, 135, pp.65-72. ⟨10.1016/j.theriogenology.2019.06.011⟩
Journal articles hal-02627338v1

Identification by proteomics of oviductal sperm-interacting proteins

Julie Lamy , Perrine Nogues , Lucie Combes-Soia , Guillaume Tsikis , Valérie Labas
Reproduction -Cambridge- Supplement-, 2018, 155 (5), pp.457-466. ⟨10.1530/REP-17-0712⟩
Journal articles hal-02625186v1
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Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle

Priscila Bertevello , Ana-Paula Teixeira-Mechin , Alexandre Seyer , Anais Carvalho , Valérie Labas
International Journal of Molecular Sciences, 2018, 19 (10), pp.3261. ⟨10.3390/ijms19103261⟩
Journal articles hal-02628741v1

Binder of Sperm Proteins 1 and 5 have contrasting effects on the capacitation of ram spermatozoa

Taylor Pini , Simon P de Graaf , Xavier Druart , Guillaume Tsikis , Valérie Labas
Biology of Reproduction, 2018, 98 (6), pp.765-775. ⟨10.1093/biolre/ioy032⟩
Journal articles hal-02626316v1

Binder of Sperm Proteins protect ram spermatozoa from freeze-thaw damage

Taylor Pinia , Kiri Farmer , Xavier Druart , Ana-Paula Teixeira-Gomes , Guillaume Tsikis
Cryobiology, 2018, 82, pp.78-87. ⟨10.1016/j.cryobiol.2018.04.005⟩
Journal articles hal-02625187v1
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Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix

T. Kryza ,