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Virgile Adam

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  • http://www.virgile-adam.com/

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Photophysical Studies at Cryogenic Temperature Reveal a Novel Photoswitching Mechanism of rsEGFP2

Angela Mantovanelli , Oleksandr Glushonkov , Virgile Adam , Jip Wulffelé , Daniel Thédié
Journal of the American Chemical Society, 2023, 145 (27), pp.14636-14646. ⟨10.1021/jacs.3c01500⟩
Journal articles hal-04162903v1
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Structural heterogeneity in a phototransformable fluorescent protein impacts its photochemical properties

Arijit Maity , Jip Wulffelé , Isabel Ayala , Adrien Favier , Virgile Adam
Advanced Science, 2023, pp.e2306272. ⟨10.1002/advs.202306272⟩
Journal articles hal-04426227v1
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Rational control of off‐state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement

Virgile Adam , Kyprianos Hadjidemetriou , Nickels Jensen , Robert L. Shoeman , Joyce Woodhouse
ChemPhysChem, 2022, 23 (19), pp.e202200192. ⟨10.1002/cphc.202200192⟩
Journal articles hal-03758975v1
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Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy

Nina Eleni Christou , Karine Giandoreggio-Barranco , Isabel Ayala , Oleksandr Glushonkov , Virgile Adam
Journal of the American Chemical Society, 2021, 143 (19), pp.7521-7530. ⟨10.1021/jacs.1c02442⟩
Journal articles hal-03328569v1
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Supramolecular assembly of the Escherichia coli LdcI upon acid stress

Matthew Jessop , Clarissa Liesche , Jan Felix , Ambroise Desfosses , Megghane Baulard
Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (2), pp.e2014383118. ⟨10.1073/pnas.2014383118⟩
Journal articles hal-03112998v1
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Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State

Elke de Zitter , Jacqueline Ridard , Daniel Thédié , Virgile Adam , Bernard C. Levy
Journal of the American Chemical Society, 2020, 142 (25), pp.10978-10988. ⟨10.1021/jacs.0c01880⟩
Journal articles hal-02895781v1
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Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy.

Joyce Woodhouse , Gabriela Nass Kovacs , Nicolas Coquelle , Lucas M. Uriarte , Virgile Adam
Nature Communications, 2020, 11 (1), pp.741. ⟨10.1038/s41467-020-14537-0⟩
Journal articles hal-02498645v1

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

Elke de Zitter , Daniel Thédié , Viola Mönkemöller , Siewert Hugelier , Joël Beaudouin
Nature Methods, 2019, 16 (8), pp.707-710. ⟨10.1038/s41592-019-0462-3⟩
Journal articles hal-02270788v1
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NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein

Nina Eleni Christou , Isabel Ayala , Karine Giandoreggio-Barranco , Martin Byrdin , Virgile Adam
Biophysical Journal, 2019, 117 (11), pp.2087-2100. ⟨10.1016/j.bpj.2019.10.035⟩
Journal articles hal-02389522v1
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Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography

Nicolas Coquelle , Michel Sliwa , Joyce Woodhouse , Giorgio Schirò , Virgile Adam
Nature Chemistry, 2018, 10 (1), pp.31-37. ⟨10.1038/nchem.2853⟩
Journal articles hal-01618533v1

Photoswitching of Green mEos2 by Intense 561 nm Light Perturbs Efficient Green-to-Red Photoconversion in Localization Microscopy

Daniel Thédié , Romain Berardozzi , Virgile Adam , Dominique Bourgeois
Journal of Physical Chemistry Letters, 2017, 8 (18), pp.4424 - 4430. ⟨10.1021/acs.jpclett.7b01701⟩
Journal articles hal-01592521v1

Serial femtosecond crystallography and ultrafast absorption spectroscopy of the photoswitchable fluorescent protein IrisFP

Jacques-Philippe Colletier , M. Sliwa , François-Xavier Gallat , M. Sugahara , Virginia Guillon
Journal of Physical Chemistry Letters, 2016, 7 (5), pp.882 - 887. ⟨10.1021/acs.jpclett.5b02789⟩
Journal articles hal-01285611v1
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Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm

Mariam El Khatib , Alexandre Martins , Dominique Bourgeois , Jacques-Philippe Colletier , Virgile Adam
Scientific Reports, 2016, 6, ⟨10.1038/srep18459⟩
Journal articles hal-01280266v1
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Arginine 66 Controls Dark-State Formation in Green-to-Red Photoconvertible Fluorescent Proteins

Romain Berardozzi , Virgile Adam , Alexandre Martins , Dominique Bourgeois
Journal of the American Chemical Society, 2016, 138 (2), pp.558-565. ⟨10.1021/jacs.5b09923⟩
Journal articles hal-01266594v1
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Remodeling of the Z-Ring Nanostructure during the Streptococcus pneumoniae Cell Cycle Revealed by Photoactivated Localization Microscopy.

Maxime Jacq , Virgile Adam , Dominique Bourgeois , Christine Moriscot , Anne-Marie Di Guilmi
mBio, 2015, 6 (4), pp.e01108-15
Journal articles hal-01199165v1

Rational design of enhanced photoresistance in a photoswitchable fluorescent protein

Chenxi Duan , Martin Byrdin , Mariam El Kathib , Xavier Henry , Virgile Adam
Methods and Applications in Fluorescence, 2015, 3 (1), pp.XXX. ⟨10.1088/2050-6120/3/1/014004⟩
Journal articles hal-01161782v1

La microscopie de fluorescence à super-résolution : un outil révolutionnaire pour imager le vivant à l'échelle nanométrique.

Virgile Adam , Dominique Bourgeois
Photoniques, 2015, 74, pp.34-39
Journal articles hal-01158907v1
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In cellulo Evaluation of Phototransformation Quantum Yields in Fluorescent Proteins Used As Markers for Single-Molecule Localization Microscopy

Sergiy Avilov , Romain Berardozzi , Mudalige S. Gunewardene , Virgile Adam , Samuel T. Hess
PLoS ONE, 2014, 9 (6), pp.Volume 9 | Issue 6 | e98362. ⟨10.1371/journal.pone.0098362⟩
Journal articles hal-01093348v1
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Phototransformable fluorescent proteins: Future challenges.

Virgile Adam , Romain Berardozzi , Martin Byrdin , Dominique Bourgeois
Current Opinion in Chemical Biology, 2014, 20, pp.92-102
Journal articles hal-01093360v1

Excited state dynamics of the photoconvertible fluorescent protein Kaede revealed by ultrafast spectroscopy

E. Fron , M. Sliwa , Virgile Adam , J. Michiels , Susana Rocha
Photochemical & Photobiological Sciences , 2014, 13, pp.867 - 874. ⟨10.1039/c3pp50335f⟩
Journal articles hal-01113460v1

Phototransformable fluorescent proteins: which one for which application?

Virgile Adam
Histochemistry and Cell Biology, 2014, 142 (1), pp.19-41
Journal articles hal-01131208v1

Structural basis of photoswitching in fluorescent proteins.

Chenxi Duan , Virgile Adam , Martin Byrdin , Dominique Bourgeois
Methods in Molecular Biology, 2014, 1148, pp.177-202
Journal articles hal-01131245v1
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Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2

Eduard Fron , Mark van Der Auweraer , Benjamien Moeyaert , Jan Michiels , Hideaki Mizuno
Journal of Physical Chemistry B, 2013, 117 (8), pp.2300 - 2313. ⟨10.1021/jp309219m⟩
Journal articles hal-01662511v1

Structural evidence for a two-regime photobleaching mechanism in a reversibly switchable fluorescent protein.

Chenxi Duan , Virgile Adam , Martin Byrdin , Jacqueline Ridard , Sylvie Kieffer-Jaquinod
Journal of the American Chemical Society, 2013, 135 (42), pp.15841-50
Journal articles hal-01322350v1

Structural Evidence for a Two-Regime Photobleaching Mechanism in a Reversibly Switchable Fluorescent Protein

Chenxi Duan , Virgile Adam , Martin Byrdin , Jacqueline Ridard , Sylvie Kieffer-Jaquinod
Journal of the American Chemical Society, 2013, 135 (42), pp.15841 - 15850. ⟨10.1021/ja406860e⟩
Journal articles hal-02650196v1

Reversible photoswitching in fluorescent proteins: A mechanistic view

Dominique Bourgeois , Virgile Adam
IUBMB Life, 2012, 64 (6), pp.482-491. ⟨10.1002/iub.1023⟩
Journal articles hal-02389714v1

Photoactivated structural dynamics of fluorescent proteins

Dominique Bourgeois , Aline Regis-Faro , Virgile Adam
Biochemical Society Transactions, 2012, 40 (3), pp.531-538. ⟨10.1042/BST20120002⟩
Journal articles hal-02389719v1

From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family

Jörg Wiedenmann , Susan Gayda , Virgile Adam , Franz Oswald , Karin Nienhaus
Journal of Biophotonics, 2011, 4 (6), pp.377-390. ⟨10.1002/jbio.201000122⟩
Journal articles hal-02389707v1
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Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications

Virgile Adam , Benjamien Moeyaert , Charlotte c. David , Hideaki Mizuno , Mickaël Lelimousin
Chemistry and Biology, 2011, 18 (10), pp.1241-1251. ⟨10.1016/j.chembiol.2011.08.007⟩
Journal articles hal-02389699v1
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The Nature of Transient Dark States in a Photoactivatable Fluorescent Protein

Arijit Roy , Martin J Field , Virgile Adam , Dominique Bourgeois
Journal of the American Chemical Society, 2011, 133 (46), pp.18586-18589. ⟨10.1021/ja2085355⟩
Journal articles hal-02389684v1

Low-temperature switching by photoinduced protonation in photochromic fluorescent proteins

Aline Regis Faro , Virgile Adam , Philippe Carpentier , Claudine Darnault , Dominique Bourgeois
Photochemical & Photobiological Sciences , 2010, 9 (2), pp.254. ⟨10.1039/b9pp00121b⟩
Journal articles hal-02389665v1

Data storage based on photochromic and photoconvertible fluorescent proteins

Virgile Adam , Hideaki Mizuno , Alexei Grichine , Jun-Ichi Hotta , Yutaka Yamagata
African Journal of Biotechnology, 2010, 149 (4), pp.289-298. ⟨10.1016/j.jbiotec.2010.04.001⟩
Journal articles hal-02389676v1

Fine Tuning the Optical Properties of Green to Red Photoconvertible Fluorescent Proteins

Karin Nienhaus , Virgile Adam , Dominique Bourgeois , G. Ulrich Nienhaus
Biophysical Journal, 2010, 98 (3), pp.413a. ⟨10.1016/j.bpj.2009.12.2228⟩
Journal articles hal-02389667v1

Photoconversion of the Fluorescent Protein EosFP: A Hybrid Potential Simulation Study Reveals Intersystem Crossings

Mickaël Lelimousin , Virgile Adam , G. Ulrich Nienhaus , Dominique Bourgeois , Martin J Field
Journal of the American Chemical Society, 2009, 131 (46), pp.16814-16823. ⟨10.1021/ja905380y⟩
Journal articles hal-02389649v1
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Structural basis of X-ray-induced transient photobleaching in a photoactivatable green fluorescent protein.

Virgile Adam , Philippe Carpentier , Sebastien Violot , Mickaël Lelimousin , Claudine Darnault
Journal of the American Chemical Society, 2009, 131 (50), pp.18063-5. ⟨10.1021/ja907296v⟩
Journal articles hal-00474158v1
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Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme.

Gergely Katona , Philippe Carpentier , Vincent Nivière , Patricia Amara , Virgile Adam
Science, 2007, pp.449-53
Journal articles hal-01075775v1
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Detoxification of superoxide without production of H2O2: antioxidant activity of superoxide reductase complexed with ferrocyanide.

Fernando P Molina-Heredia , Chantal Houée-Levin , C. Berthomieu , Danièle Touati , Emilie Tremey
Proceedings of the National Academy of Sciences of the United States of America, 2006, 103 (40), pp.14750-5. ⟨10.1073/pnas.0510828103⟩
Journal articles hal-01075778v1

The crystal structure of Mycobacterium tuberculosis thymidylate kinase in complex with 3'-azidodeoxythymidine monophosphate suggests a mechanism for competitive inhibition.

Emanuela Fioravanti , Virgile Adam , Hélène Munier-Lehmann , Dominique Bourgeois
Biochemistry, 2005, 44 (1), pp.130-7. ⟨10.1021/bi0484163⟩
Journal articles pasteur-00166954v1
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Structure of superoxide reductase bound to ferrocyanide and active site expansion upon X-ray-induced photo-reduction.

Virgile Adam , Antoine Royant , Vincent Nivière , Fernando P Molina-Heredia , Dominique Bourgeois
Structure, 2004, 12, pp.1729-40. ⟨10.1016/j.str.2004.07.013⟩
Journal articles hal-01075786v1
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Etudes photo-physiques et ingénierie des protéines fluorescentes photo-transformables

Virgile Adam
Biophysique. Université Grenoble Alpes, 2023
Habilitation à diriger des recherches tel-04322660v1