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Wassim El Nemer

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#### Professional record - 2020-present: Scientific Director at EFS (The French Blood Establishment) - 2012-2020: Group leader at Inserm UMR\_S 1134 “Integrated Biology of the Red Cell” - 2014-2020: Head of the “Red Cell Core Facility” at UMR\_S 1134/INTS - 2002-2020: Tenured scientist at Inserm UMR\_S 1134 “Red Blood Cell Integrated Biology” - 2001-2002: Post-doctoral fellow at the University of California San Francisco - 1999-2001: Assistant Professor at the University of Paris Diderot – Paris 7 #### Education - 2011: Habilitation à Diriger les Recherches (HDR) degree, qualifying for research and PhD students supervision - 2000: PhD at the University of Paris Diderot – Paris 7 (Major: Human Genetics) - 1996: Master of Science in Human Genetics at the University of Paris Diderot – Paris 7 #### Research and academia - Since 2000: supervisor of 9 PhD students and > 20 MSc students - Teaching in PhD and MSc courses - Associate Editor of “Frontiers in Physiology”, “Clinical Hemorheology and Microcirculation”, “Transfusion Clinique et Biologique”, “Blood Cells, Molecules and Diseases” - Member of the Amercian Society of Hematology (ASH), the European Haematology Association (EHA), the French Society of Haematology (SFH), the Red Cell and Iron Club (CGRF) and the American Red Cell Club - Reviewer for Blood, Haematologica, The Journal of Biological Chemistry, Plos One, Frontiers… - Abstract reviewing: ASH 2020 & 2021, SFH 2021, SFTS 2021, SFH 2022 - Coordinating reviewer for ASH 2021 section 113-Hemoglobinopathies, Excluding Thalassemia: Basic and Translational #### Conference organisation - Co-Chair of the CGRF 2022 meeting, 28-30 September 2022 (Presqu’Île de Giens, France) - Co-chair of the GR-Ex – ESH Training course on “Red Blood Cells: Genesis and Pathophysiology”, 18-20 April 2018 (Paris – France) ( - Co-chair of the GR-Ex – ESH Training course on “Red Blood Cells: Genesis and Pathophysiology”, 23-26 April 2014 (Chantilly – France) ( #### Awards - 2010 & 2018: a 4-year “Scientific Excellence Award” (PES) from Inserm - 2005: “Young researcher award” from the Red Cell and Iron Club - 2001: Human Frontier Science Program Long-Term fellowship awardee #### Publications and communication activities - > 70 published articles in peer-reviewed journals - > 60 invited and selected oral presentations in national and international conferences #### European grants - EVIDENCE: Erythrocytes properties and viability in dependence of flow and extra-cellular Environment. 2019. ITN Marie-Curie H2020 (Partner) - RELEVANCE: Regulation of red cell life-span, erythropoiesis, survival, senescence and clearance. 2015. ITN Marie-Curie H2020 (Partner) #### ANR grant - IRIS: Investigating ineffective erythropoiesis in sickle cell disease. ANR 2021 (Coordinator)


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Base-editing-mediated dissection of a γ-globin cis-regulatory element for the therapeutic reactivation of fetal hemoglobin expression

Panagiotis Antoniou , Giulia Hardouin , Pierre Martinucci , Giacomo Frati , Tristan Felix
Nature Communications, 2022, 13 (1), pp.6618. ⟨10.1038/s41467-022-34493-1⟩
Journal articles mnhn-03868573v1
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Altered Ca2+ Homeostasis in Red Blood Cells of Polycythemia Vera Patients Following Disturbed Organelle Sorting during Terminal Erythropoiesis

Ralfs Buks , Tracy Dagher , Maria Giustin Rotordam , David Monedero Alonso , Sylvie Cochet
Cells, 2022, 11 (1), pp.49. ⟨10.3390/cells11010049⟩
Journal articles hal-03523607v1

Combination of lentiviral and genome editing technologies for the treatment of sickle cell disease

Sophie Ramadier , Anne Chalumeau , Tristan Felix , Nadia Othman , Sherazade Aknoun
Molecular Therapy, 2022, 30 (1), pp.145-163. ⟨10.1016/j.ymthe.2021.08.019⟩
Journal articles hal-03531070v1
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Characterisation of Asp669Tyr Piezo1 cation channel activity in red blood cells: an unexpected phenotype

Laurent Pérès , David Monedero Alonso , Morgane Nudel , Martin Figeac , Judith Bruge
British Journal of Haematology, 2021, ⟨10.1111/bjh.17467⟩
Journal articles hal-03242645v1
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Lysophosphatidic Acid-Activated Calcium Signaling Is Elevated in Red Cells from Sickle Cell Disease Patients

Jue Wang , Laura Hertz , Sandra Ruppenthal , Wassim El Nemer , Philippe Connes
Cells, 2021, 10 (2), pp.456. ⟨10.3390/cells10020456⟩
Journal articles hal-03531054v1

Ineffective erythropoiesis in sickle cell disease: new insights and future implications

Wassim El Nemer , Auria Godard , Sara El Hoss
Current Opinion in Hematology, 2021, 28 (3), pp.171-176. ⟨10.1097/MOH.0000000000000642⟩
Journal articles hal-03291949v1
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ABCG2 Is Overexpressed on Red Blood Cells in Ph-Negative Myeloproliferative Neoplasms and Potentiates Ruxolitinib-Induced Apoptosis

Ralfs Buks , Mégane Brusson , Sylvie Cochet , Tatiana Galochkina , Bruno Cassinat
International Journal of Molecular Sciences, 2021, 22 (7), pp.3530. ⟨10.3390/ijms22073530⟩
Journal articles hal-03531056v1
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Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype

Leslie Weber , Giacomo Frati , Tristan Felix , Giulia Hardouin , Antonio Casini
Science Advances , 2020, 6 (7), pp.eaay9392. ⟨10.1126/sciadv.aay9392⟩
Journal articles mnhn-03100446v1

Plasma microparticles of sickle patients during crisis or taking hydroxyurea modify endothelium inflammatory properties

Yohann Garnier , Séverine Ferdinand , Marie Garnier , Kizzy-Clara Cita , Régine Hierso
Blood, 2020, 136 (2), pp.247-256. ⟨10.1182/blood.2020004853⟩
Journal articles hal-03091322v1
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Characterization of red blood cell microcirculatory parameters using a bioimpedance microfluidic device

Tieying Xu , Maria A Lizarralde-Iragorri , Jean Roman , Rasta Ghasemi , Jean-Pierre Lefevre
Scientific Reports, 2020, ⟨10.1038/s41598-020-66693-4⟩
Journal articles hal-02872830v1
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Oxidative stress activates red cell adhesion to laminin in sickle cell disease

Maria Alejandra Lizarralde-Iragorri , Sophie Lefevre , Sylvie Cochet , Sara El Hoss , Valentine Brousse
Haematologica, 2020, 106 (9), pp.haematol.2020.261586. ⟨10.3324/haematol.2020.261586⟩
Journal articles hal-02929195v1

Dimerization and phosphorylation of Lutheran/basal cell adhesion molecule are critical for its function in cell migration on laminin

Anna Guadall , Sylvie Cochet , Olivier Renaud , Yves Colin , Caroline Le van Kim
Journal of Biological Chemistry, 2019, 294 (41), pp.14911-14921. ⟨10.1074/jbc.RA119.007521⟩
Journal articles hal-02416861v1

Reticulocyte and red blood cell deformation triggers specific phosphorylation events

Pedro Moura , Maria Lizarralde Iragorri , Olivier Français , Bruno Le Pioufle , Johannes Dobbe
Blood Advances, 2019, 3 (17), pp.2653-2663. ⟨10.1182/bloodadvances.2019000545⟩
Journal articles hal-02289553v1

Absolute proteome quantification of highly purified populations of circulating reticulocytes and mature erythrocytes

Emilie-Fleur Gautier , Marjorie Leduc , Sylvie Cochet , Karine Bailly , Catherine Lacombe
Blood Advances, 2018, 2 (20), pp.2646-2657. ⟨10.1182/bloodadvances.2018023515⟩
Journal articles hal-02365675v1

Band 3 phosphorylation induces irreversible alterations of stored red blood cells

Slim Azouzi , Marc Romana , Nobuto Arashiki , Yuichi Takakuwa , Wassim El Nemer
American Journal of Hematology, 2018, 93 (5), pp.E110 - E112. ⟨10.1002/ajh.25044⟩
Journal articles hal-01825593v1

A microfluidic approach to study the effect of mechanical stress on erythrocytes in sickle cell disease

Maria Alejandra Lizarralde Iragorri , Sara El Hoss, , Valentine Brousse , Sophie Lefevre , Michael Dussiot
Lab on a Chip, 2018, 18 (19), pp.2975-2984. ⟨10.1039/C8LC00637G⟩
Journal articles hal-01875109v1

An Optimized Lentiviral Vector Efficiently Corrects the Human Sickle Cell Disease Phenotype

L. Weber , V. Poletti , E. Magrin , C. Antoniani , S. Martin
Mol Ther Methods Clin Dev, 2018, 10, pp.268-280. ⟨10.1016/j.omtm.2018.07.012⟩
Journal articles hal-02880821v1

Impact of hydroxycarbamide and interferon-alpha on red cell adhesion and membrane protein expression in polycythemia vera

Megane Brusson , Maria de Grandis , Sylvie Cochet , Sylvain Bigot , Mickael Marin
Haematologica, 2018, 103 (6), pp.972-981. ⟨10.3324/haematol.2017.182303⟩
Journal articles hal-02117996v1

Induction of fetal hemoglobin synthesis by CRISPR/Cas9-mediated editing of the human beta-globin locus

C. Antoniani , V. Meneghini , A. Lattanzi , T. Felix , O. Romano
Blood, 2018, 131, pp.1960-1973. ⟨10.1182/blood-2017-10-811505⟩
Journal articles hal-02880783v1

Prognostic factors of disease severity in infants with sickle cell anemia: A comprehensive longitudinal cohort study

Valentine Brousse , Sara El Hoss , Naim Bouazza , Cécile Arnaud , Françoise Bernaudin
American Journal of Hematology, 2018, 93 (11), pp.1411-1419. ⟨10.1002/ajh.25260⟩
Journal articles hal-02416879v1
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Gene Therapy in a Patient with Sickle Cell Disease

Jean-Antoine Ribeil , Salima Hacein-Bey-Abina , Emmanuel Payen , Alessandra Magnani , Michaela Semeraro
New England Journal of Medicine, 2017, 376 (9), pp.848 - 855. ⟨10.1056/NEJMoa1609677⟩
Journal articles inserm-01834781v1

The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension

Annalisa Lattanzi , Vasco Meneghini , Giulia Pavani , Fatima Amor , Sophie Ramadier
Cell, 2017, 169 (4), pp.693-707.e14. ⟨10.1016/j.cell.2017.04.013⟩
Journal articles hal-03588462v1

Effects of Poloxamer 188 on red blood cell membrane properties in sickle cell anaemia

Barbara Sandor , Mickaël Marin , Claudine Lapouméroulie , Miklos Rabaï , Sophie Lefevre
British Journal of Haematology, 2016, 173 (1), pp.145 - 149. ⟨10.1111/bjh.13937⟩
Journal articles hal-01881668v1

A biomimetic microfluidic chip to study the circulation and mechanical retention of red blood cells in the spleen

Julien Picot , Papa Alioune Ndour , Sophie D. Lefevre , Wassim El Nemer , Harvey Tawfik
American Journal of Hematology, 2015, ⟨10.1002/ajh.23941⟩
Journal articles hal-01113050v1
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Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria

Julien Duez , John P. Holleran , Papa Alioune Ndour , Sasdekumar Loganathan , Pascal Amireault
Antimicrobial Agents and Chemotherapy, 2015, pp.AAC.05030-14. ⟨10.1128/AAC.05030-14⟩
Journal articles hal-01158996v1
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JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway.

Maria de Grandis , Marie Cambot , Marie-Paule Wautier , Bruno Cassinat , Christine Chomienne
Blood, 2013, 121 (4), pp.658-65. ⟨10.1182/blood-2012-07-440487⟩
Journal articles inserm-00821109v1

Human induced pluripotent stem cells can reach complete terminal maturation In vivo and in vitro evidence in the erythropoietic differentiation model

L. Kobar , F. Yates , N. Oudrhiri , A. Francina , L. Kiger
Haematologica, 2012, 97 (12), pp.1795-1803. ⟨10.3324/haematol.2011.055566⟩
Journal articles hal-02920223v1
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Novel role for the Lu/BCAM-spectrin interaction in actin cytoskeleton reorganization

Emmanuel Collec , Marie-Christine Lecomte , Wassim El-Nemer , Yves Colin , Caroline Le van Kim
Biochemical Journal, 2011, 436 (3), pp.699-708. ⟨10.1042/BJ20101717⟩
Journal articles hal-00596275v1
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Aggregation of mononuclear and red blood cells through an {alpha}4{beta}1-Lu/basal cell adhesion molecule interaction in sickle cell disease.

Vicky Chaar , Julien Picot , Olivier Renaud , Pablo Bartolucci , Ruben Nzouakou
Haematologica, 2010, 95 (11), pp.1841-8. ⟨10.3324/haematol.2010.026294⟩
Journal articles inserm-00821120v1
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Decreased sickle red blood cell adhesion to laminin by hydroxyurea is associated with inhibition of Lu/BCAM protein phosphorylation.

Pablo Bartolucci , Vicky Chaar , Julien Picot , Dora Bachir , Anoosha Habibi
Blood, 2010, 116 (12), pp.2152-9. ⟨10.1182/blood-2009-12-257444⟩
Journal articles inserm-00821117v1
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Ubc9 interacts with Lu/BCAM adhesion glycoproteins and regulates their stability at the membrane of polarized MDCK cells

Emmanuel Collec , Wassim El Nemer , Emilie Gauthier , Pierre Gane , Marie-Christine Lecomte
Biochemical Journal, 2006, 402 (2), pp.311-319. ⟨10.1042/BJ20060861⟩
Journal articles hal-00478614v1

Proteomic analysis of the tetraspanin web using LC-ESI-MS/MS and MALDI-FTICR-MS.

M. André , Jean-Pierre Le Caer , C. Greco , S. Planchon , W. El Nemer
Proteomics, 2006, 6 (5), pp.1437-1449. ⟨10.1002/pmic.200500180⟩
Journal articles hal-00904619v1
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Applying successive mechanical stresses on circulating cells using microfluidic vascular network to assess sickled red blood cells rigidity

Tieying Xu , Maria A. Lizarralde-Iragorri , Olivier Français , Wassim El Nemer , Bruno Le Pioufle
26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Oct 2022, Hangzhou, China
Conference papers hal-03831982v1
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Different miniaturization systems of electrical biosensing and data acquisition during biological cell transiting within vessel size microchannel

Tieying Xu , Maria Lizarralde Iragorri , Jean Roman , Emile Martincic , Valentine Brousse
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences ( MicroTAS 2021 ), Oct 2021, Palm Springs, United States
Conference papers hal-03821200v1
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Spherocytes flow behaviours in microrestriction, compared by normal red blood cells of different heat treatment levels

Tieying Xu , Maria Lizarralde Iragorri , Jean Roman , Emile Martincic , Valentine Brousse
MicroTAS 2021, Oct 2021, Palm Springs, United States
Conference papers hal-03821199v1
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A microfluidic device to statistically determine the distribution of sickle red cell subpopulations using bioimpedance

Tieying Xu , Maria A Lizarralde-Iragorri , Jean Roman , Emile Martincic , Valentine Brousse
Transducers, 2021, on-line, France
Conference papers hal-03260796v1
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Electrical Sensing of sickled red blood cells subpopulations in microfluidic device

Tieying Xu , Maria A Lizarralde-Iragorri , Jean Roman , Emile Martincic , Valentine Brousse
MicroTAS, Oct 2020, on-line, France
Conference papers hal-03078878v1
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Electrical Detection of the Mechanical Alteration of Sickling Red Blood Cells within a Microfluidic Capillary Network

Xu Tieying , Maria Lizarralde , Jean Roman , Wassim El Nemer , Bruno Le Pioufle
MicroTAS, Oct 2019, Basel, Switzerland
Conference papers hal-02387976v1
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Microfluidic device using reusable parylen-pdms packaging for the red blood cell transit time analysis in mechanical constrictions, using impedance measurement

Xu Tieying , Maria Lizarralde , Wassim El Nemer , Bruno Le Pioufle , Olivier Français
MicroTAS, Nov 2018, Kaohsiung, Taiwan
Conference papers hal-01972640v1
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Monitoring Biological Cell Flow within a Mimicking Capillary Device with Impedance Measurement

Tieying Xu, , Maria Lizarralde, , Wassim El Nemer , Bruno Le Pioufle , Olivier Français
Eurosensors, Sep 2017, Paris, France. pp.3 - 6, ⟨10.3390/proceedings1040517⟩
Conference papers hal-01592142v1

A microfluidic device to study red blood cell deformability

Wassim El Nemer , Papa Alioune Ndour , Sophie D. Lefevre , Harvey Tawfik , Julien Picot
4th European Conference on Microfluidics, Dec 2014, Limerick, Ireland
Conference papers hal-01113061v1