Didier Flament

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Publications

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On the origins and variation of nucleotide skews of archaeal genomes

Adrien Paravel , Clémence Mottez , Romain Puech , Didier Flament , Hubert F Becker et al.

Frontiers in Microbiology, 2026, 17, pp.1727296. ⟨10.3389/fmicb.2026.1727296⟩

Article dans une revue hal-05543510v1
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Wide diversity and complex evolution of M42 aminopeptidases with contrasted functional properties in Archaea

Emilie Chagny , Najwa Taib , Daphna Fenel , Eric Girard , Simonetta Gribaldo et al.

Molecular Biology and Evolution, 2025, 42 (10), ⟨10.1093/molbev/msaf233⟩

Article dans une revue hal-05274166v1
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Cooperation between two modes for DNA replication initiation in the archaeon <i>Thermococcus barophilus</i>

Logan Mc Teer , Yann Moalic , Valérie Cueff-Gauchard , Ryan Catchpole , Gaëlle Hogrel et al.

mBio, 2024, 15, ⟨10.1128/mbio.03200-23⟩

Article dans une revue hal-04546373v1
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Evolutionary and functional insights into the Ski2-like helicase family in Archaea: a comparison of Thermococcales ASH-Ski2 and Hel308 activities

Manon Batista , Petra Langendijk-Genevaux , Marta Kwapisz , Isabelle Canal , Duy khanh Phung et al.

NAR Genomics and Bioinformatics, 2024, 6 (1), lqae026 (16p.). ⟨10.1093/nargab/lqae026⟩

Article dans une revue hal-04573206v1
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Cooperation between two modes for DNA replication initiation in the archaeon Thermococcus barophilus

Logan Mc Teer , Yann Moalic , Valérie Cueff-Gauchard , Ryan Catchpole , Gaëlle Hogrel et al.

mBio, 2024, 15 (4), ⟨10.1128/mbio.03200-23⟩

Article dans une revue hal-04728152v1
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DNA polymerization in icy moon abyssal pressure conditions

Lorenzo Carré , Ghislaine Henneke , Etienne Henry , Didier Flament , Éric Girard et al.

Astrobiology, 2023, pp.0201. ⟨10.1089/ast.2021.0201⟩

Article dans une revue hal-03944643v1
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DNA-binding mechanism and evolution of replication protein A

Clément Madru , Markel Martínez-Carranza , Sébastien Laurent , Alessandra Alberti , Maelenn Chevreuil et al.

Nature Communications, 2023, 14 (1), pp.2326. ⟨10.1038/s41467-023-38048-w⟩

Article dans une revue hal-04112308v1
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Structural and functional determinants of the archaeal 8-oxoguanine-DNA glycosylase AGOG for DNA damage recognition and processing

Coste Franck , Stéphane Goffinont , Julien Cros , Virginie Gaudon , Guérin Martine et al.

Nucleic Acids Research, 2022, ⟨10.1093/nar/gkac932⟩

Article dans une revue hal-03841526v1
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Characterization of a small tRNA ‐binding protein that interacts with the archaeal proteasome complex

Gaëlle Hogrel , Laura Marino-Puertas , Sébastien Laurent , Ziad Ibrahim , Jacques Covès et al.

Molecular Microbiology, 2022, Bacterial macromolecular machineries, 118 (1-2), pp.16-29. ⟨10.1111/mmi.14948⟩

Article dans une revue hal-03701716v1
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Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase

Mirna Hajj , Petra Langendijk-Genevaux , Manon Batista , Yves Quentin , Sébastien Laurent et al.

Biomolecules, 2021, 11 (7), pp.950. ⟨10.3390/biom11070950⟩

Article dans une revue hal-03365138v1
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RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery

Duy Khanh Phung , Clarisse Etienne , Manon Batista , Petra Langendijk-Genevaux , Yann Moalic et al.

Nucleic Acids Research, 2020, 48 (7), pp.3832-3847. ⟨10.1093/nar/gkaa052⟩

Article dans une revue hal-03028340v1
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Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea

Gaelle Hogrel , Yang Lu , Nicolas Alexandre , Audrey Bossé , Remi Dulermo et al.

Biomolecules, 2020, 10 (7), 1045 (17p.). ⟨10.3390/biom10071045⟩

Article dans une revue hal-04202612v1
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Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus

Gaëlle Hogrel , Yang Lu , Sébastien Laurent , Etienne Henry , Clarisse Etienne et al.

Nucleic Acids Research, 2018, 46 (11), pp.5651-5663. ⟨10.1093/nar/gky322⟩

Article dans une revue hal-02390072v1
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Development of an Effective 6-Methylpurine Counterselection Marker for Genetic Manipulation in Thermococcus barophilus

Tiphaine Birien , Axel Thiel , Ghislaine Henneke , Didier Flament , Yann Moalic et al.

Genes, 2018, 9 (2), pp.77. ⟨10.3390/genes9020077⟩

Article dans une revue hal-04202052v1
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Genetic Manipulations of the Hyperthermophilic Piezophilic Archaeon Thermococcus barophilus

Axel Thiel , Grégoire Michoud , Yann Moalic , Didier Flament , Mohamed Jebbar

Applied and Environmental Microbiology, 2014, 80 (7), pp.2299-2306. ⟨10.1128/AEM.00084-14⟩

Article dans une revue hal-01111536v1

Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of eIF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive

Sébastien Laurent , Adrien Richard , Odile Mulner-Lorillon , Julia Morales , Didier Flament et al.

Frontiers in Genetics, 2014, 5, pp.117. ⟨10.3389/fgene.2014.00117⟩

Article dans une revue hal-01079758v1
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Genetic manipulations of the hyperthermophilic piezophilic archaeon Thermococcus barophilus.

Axel Thiel , Grégoire Michoud , Yann Moalic , Didier Flament , Mohamed Jebbar

Applied and Environmental Microbiology, 2014, 80 (7), pp.2299-306. ⟨10.1128/AEM.00084-14⟩

Article dans une revue hal-01019712v1

An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs

Pierre-François Pluchon , Thomas Fouqueau , Christophe Crezé , Sébastien Laurent , Julien Briffotaux et al.

PLoS ONE, 2013, 8 (11), pp.1-15. ⟨10.1371/journal.pone.0079707⟩

Article dans une revue hal-00911795v1

Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels.

Christophe Creze , Alessio Ligabue , Sébastien Laurent , Roxane Lestini , Sergey P. Laptenok et al.

Journal of Biological Chemistry, 2012, 287 (19), pp.15648-60. ⟨10.1074/jbc.M112.346361⟩

Article dans une revue hal-00808519v1

Structure and function of a novel endonuclease acting on branched DNA substrates

C. Creze , Roxane Lestini , Joëlle Kühn , Alessio Ligabue , Hubert F. Becker et al.

Biochemical Society Transactions, 2011, 39 (1), pp.145-149. ⟨10.1042/BST0390145⟩

Article dans une revue hal-00838893v1
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MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms.

Agnès Groisillier , Cécile Hervé , Alexandra Jeudy , Etienne Rebuffet , Pierre François Pluchon et al.

Microb Cell Fact, 2010, 9, pp.45. ⟨10.1186/1475-2859-9-45⟩

Article dans une revue hal-00609166v1

The glycine-rich motif of Pyrococcus abyssi DNA polymerase D is critical for protein stability.

Benoît Castrec , Sébastien Laurent , Ghislaine Henneke , Didier Flament , Jean-Paul Raffin

Journal of Molecular Biology, 2010, 396 (4), pp.840-848. ⟨10.1016/j.jmb.2010.01.006⟩

Article dans une revue hal-00609913v1

Structure and function of a novel endonuclease acting on branched DNA substrates.

Bin Ren , Joelle Kühn , Laurence Meslet-Cladiere , Julien Briffotaux , Cedric Norais et al.

EMBO Journal, 2009, 28 (16), pp.2479-89. ⟨10.1038/emboj.2009.192⟩

Article dans une revue hal-00406013v1

Binding to PCNA in Euryarchaeal DNA Replication requires two PIP motifs for DNA polymerase D and one PIP motif for DNA polymerase B

Benoît Castrec , Christophe Rouillon , Ghislaine Henneke , Didier Flament , Joël Querellou et al.

Journal of Molecular Biology, 2009, 394 (2), pp.209-218. ⟨10.1016/j.jmb.2009.09.044⟩

Article dans une revue hal-00609928v1

The heterodimeric primase from the euryarchaeon Pyrococcus abyssi: a multifunctional enzyme for initiation and repair?

Magali Le Breton , Ghislaine Henneke , Cédric Norais , Didier Flament , Hannu Myllykallio et al.

Journal of Molecular Biology, 2007, 374 (5), pp.1172-85. ⟨10.1016/j.jmb.2007.10.015⟩

Article dans une revue hal-00195212v1

Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi

Claire Geslin , Mélusine Gaillard , Didier Flament , K. Rouault , Marc Le Romancer et al.

Journal of Bacteriology, 2007, 189 (12), pp.4510-4519. ⟨10.1128/JB.01896-06⟩

Article dans une revue hal-00616744v1
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Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families

Didier Flament , Tristan Barbeyron , Murielle Jam , Philippe Potin , Mirjam Czjzek et al.

Applied and Environmental Microbiology, 2007, 73 (14), pp.4691-4694. ⟨10.1128/AEM.00496-07⟩

Article dans une revue hal-00616725v1

A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen.

Laurence Meslet-Cladiére , Cédric Norais , Joëlle Kuhn , Julien Briffotaux , Jerry W Sloostra et al.

Journal of Molecular Biology, 2007, 372 (5), pp.1137-48. ⟨10.1016/j.jmb.2007.06.056⟩

Article dans une revue hal-00193755v1

Structural insights into a new homodimeric self-activated GTPase family.

Stéphanie Gras , Valérie Chaumont , Bernard Fernandez , Philippe Carpentier , Fabienne Charrier-Savournin et al.

EMBO Reports, 2007, 8 (6), pp.569-75. ⟨10.1038/sj.embor.7400958⟩

Article dans une revue hal-00195309v1

DNA polymerase switching on homotrimeric PCNA at the replication fork of the euryarchaea Pyrococcus abyssi

Christophe Rouillon , Ghislaine Henneke , Didier Flament , Joël Querellou , Jean-Paul Raffin

Journal of Molecular Biology, 2007, 369 (2), pp.343-355. ⟨10.1016/j.jmb.2007.03.054⟩

Article dans une revue hal-00617495v1

The Hyperthermophilic Euryarchaeota Pyrococcus abyssi Likely Requires the Two DNA Polymerases D and B for DNA Replication

Ghislaine Henneke , Didier Flament , Ulrich Hübscher , Joël Querellou , Jean-Paul Raffin

Journal of Molecular Biology, 2005, 350 (1), pp.53-64. ⟨10.1016/j.jmb.2005.04.042⟩

Article dans une revue hal-05586459v1

The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours.

M. Jam , D. Flament , J. Allouch , P. Potin , Dolorès Thion et al.

Biochemical Journal, 2005, 385, pp.703-713

Article dans une revue hal-00092292v1

The endo-β-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans : two paralogue enzymes with different molecular organizations and catalytic behaviours

Murielle Jam , Didier Flament , Julie Allouch , Philippe Potin , Laurent Thion et al.

Biochemical Journal, 2005, 385 (3), pp.703-713. ⟨10.1042/BJ20041044⟩

Article dans une revue hal-05586491v1

Genetic elements of Thermococcales

D. Prieur , G. Erauso , C. Geslin , S. Lucas , M. Gaillard et al.

Biochemical Society Transactions, 2004, 32 (2), pp.184-187. ⟨10.1042/bst0320184⟩

Article dans une revue hal-05587290v1

Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas

Yannick Gueguen , Jean-Paul Cadoret , Didier Flament , Catherine Barreau-Roumiguière , Anne-Laure Girardot et al.

Gene, 2003, 303, pp.139-145. ⟨10.1016/S0378-1119(02)01149-6⟩

Article dans une revue istex hal-02498520v1

An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi

Georges Cohen , Valérie Barbe , Didier Flament , Michael Galperin , Roland Heilig et al.

Molecular Microbiology, 2003, 47 (6), pp.1495-1512. ⟨10.1046/j.1365-2958.2003.03381.x⟩

Article dans une revue hal-04141223v1

Comment on "The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1": evidence that the unique N-terminal sequence proposed comes from a maltodextrin phosphorylase

Jean-Luc Rolland , Yannick Gueguen , Didier Flament , Yann Pouliquen , Peter Street et al.

Extremophiles, 2002, 6 (4), pp.349-350. ⟨10.1007/s00792-001-0258-z⟩

Article dans une revue istex hal-05587322v1

Replication Factor C from the Hyperthermophilic Archaeon Pyrococcus abyssi Does Not Need ATP Hydrolysis for Clamp-loading and Contains a Functionally Conserved RFC PCNA-binding Domain

Ghislaine Henneke , Yannick Gueguen , Didier Flament , Philippe Azam , Joël Querellou et al.

Journal of Molecular Biology, 2002, 323 (5), pp.795-810. ⟨10.1016/s0022-2836(02)01028-8⟩

Article dans une revue hal-05587314v1

Characterization of the maltooligosyl trehalose synthase from the thermophilic archaeon Sulfolobus acidocaldarius

Yannick Gueguen , Jean-Luc Rolland , Silke Schroeck , Didier Flament , Sophie Defretin et al.

FEMS Microbiology Letters, 2001, 194 (2), pp.201-206. ⟨10.1111/j.1574-6968.2001.tb09470.x⟩

Article dans une revue hal-05587331v1

Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon <i>Pyrococcus abyssi</i>: DNA polymerases from <i>Pyrococcus abyssi</i>

Yannick Gueguen , Jean-Luc Rolland , Odile Lecompte , Philippe Azam , Gisèle Le Romancer et al.

European Journal of Biochemistry, 2001, 268 (22), pp.5961-5969. ⟨10.1046/j.0014-2956.2001.02550.x⟩

Article dans une revue hal-04094338v1

Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi

Joseph Boudrant , Jean Luc Rolland , Didier Flament , Yannick Gueguen , Jacques Dietrich

Applied and Environmental Microbiology, 2001, 67 (10), pp.4504-11. ⟨10.1128/AEM.67.10.4504-4511.2001⟩

Article dans une revue hal-03059758v1

Expression, purification, crystallization and preliminary X-ray analysis of the ι-carrageenase from Alteromonas fortis

Gurvan Michel , Didier Flament , Tristan Barbeyron , Thierry Vernet , Bernard Kloareg et al.

Acta Crystallographica Section D: Biological Crystallography, 2000, 56 (6), pp.766-768. ⟨10.1107/S0907444900004844⟩

Article dans une revue istex hal-05587498v1

Expression, purification, crystallization and preliminary X-ray analysis of the κ-carrageenase from Pseudoalteromonas carrageenovora

Gurvan Michel , Tristan Barbeyron , Didier Flament , Thierry Vernet , Bernard Kloareg et al.

Acta Crystallographica Section D: Biological Crystallography, 1999, 55 (4), pp.918-920. ⟨10.1107/s0907444998018526⟩

Article dans une revue istex hal-05587507v1

Real-Time Fluorescence Assays to Monitor DNA Polymerase Activities

Etienne Henry , Ghislaine Henneke , Didier Flament

61st Biophysical Society Annual Meeting, Feb 2017, New Orleans LA USA, United States. pp.513a, ⟨10.1016/j.bpj.2016.11.2776⟩

Communication dans un congrès hal-04461169v1