Emmanuel Margeat

51
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Publications

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Triple Labeling Resolves a GPCR Intermediate State by Using Three-Color Single Molecule FRET

Léo Bonhomme , Ecenaz Bilgen , Caroline Clerté , Jean-Philippe Pin , Philippe Rondard et al.
Journal of the American Chemical Society, 2025, 147 (21), pp.17689-17700. ⟨10.1021/jacs.4c18364⟩
Article dans une revue hal-05079126 v1
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Single-stranded DNA drives σ subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations

Rishi Kishore Vishwakarma , Nils Marechal , Zakia Morichaud , Mickaël Blaise , Emmanuel Margeat et al.
Nucleic Acids Research, 2025, 53 (7), pp.gkaf272. ⟨10.1093/nar/gkaf272⟩
Article dans une revue hal-05037511 v1
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Structural basis of orientated asymmetry in a mGlu heterodimer

Weizhu Huang , Nan Jin , Jia Guo , Cangsong Shen , Chanjuan Xu et al.
Nature Communications, 2024, 15 (1), pp.10345. ⟨10.1038/s41467-024-54744-7⟩
Article dans une revue hal-04819462 v1
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Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

Ganesh Agam , Christian Gebhardt , Milana Popara , Rebecca Mächtel , Julian Folz et al.
Nature Methods, 2023, 20 (4), pp.523 - 535. ⟨10.1038/s41592-023-01807-0⟩
Article dans une revue hal-04120543 v1
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Concerted conformational changes control metabotropic glutamate receptor activity

Nathalie Lecat-Guillet , Robert Quast , Hongkang Liu , Emmanuel Bourrier , Thor Møller et al.
Science Advances , 2023, 9 (22), pp.eadf1378. ⟨10.1126/sciadv.adf1378⟩
Article dans une revue hal-04121751 v1

Dissecting metabotropic glutamate receptor activation by single molecule FRET using minimally invasive labeling strategies

Leo Bonhomme , Robert Quast , Nathalie Lecat-Guillet , Jean-Philippe Pin , Philippe Rondard et al.
Biophysical Journal, 2023, 122 (3), pp.58a. ⟨10.1016/j.bpj.2022.11.521⟩
Article dans une revue hal-04598517 v1
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Viro-fluidics: Real-time analysis of virus production kinetics at the singlecell level

Joëlle Eid , Marius Socol , Antoine Naillon , Jérôme Feuillard , Luca Ciandrini et al.
Biophysical Reports, 2022, 2 (3), pp.100068. ⟨10.1016/j.bpr.2022.100068⟩
Article dans une revue hal-03751935 v2
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A modular spring-loaded actuator for mechanical activation of membrane proteins

A. Mills , N. Aissaoui , Damien Maurel , J. Elezgaray , F. Morvan et al.
Nature Communications, 2022, 13 (1), pp.3182. ⟨10.1038/s41467-022-30745-2⟩
Article dans une revue hal-03751001 v1
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Cryo-EM structure of transcription termination factor Rho from Mycobacterium tuberculosis reveals bicyclomycin resistance mechanism

Emmanuel Saridakis , Rishi Vishwakarma , Josephine Lai-Kee-Him , Kevin Martin , Isabelle Simon et al.
Communications Biology, 2022, 5 (1), pp.120. ⟨10.1038/s42003-022-03069-6⟩
Article dans une revue hal-03563179 v1
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FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Eitan Lerner , Anders Barth , Jelle Hendrix , Benjamin Ambrose , Victoria Birkedal et al.
eLife, 2021, 10, ⟨10.7554/elife.60416⟩
Article dans une revue hal-03191929 v1
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Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state

Anne-Marinette Cao , Robert B Quast , Fataneh Fatemi , Philippe Rondard , Jean-Philippe Pin et al.
Nature Communications, 2021, 12 (1), pp.5426. ⟨10.1038/s41467-021-25620-5⟩
Article dans une revue hal-03352901 v2
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Single-molecule FRET on its way to structural biology in live cells

Robert Quast , Emmanuel Margeat
Nature Methods, 2021, 18 (4), pp.344-345. ⟨10.1038/s41592-021-01084-9⟩
Article dans une revue hal-03191934 v1
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Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies

Thales Fernandes , Oscar Saavedra , Emmanuel Margeat , Pierre-Emmanuel Milhiet , Luca Costa
Scientific Reports, 2020, 10 (1), pp.7098. ⟨10.1038/s41598-020-62529-3⟩
Article dans une revue hal-02568884 v1
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Studying GPCR conformational dynamics by single molecule fluorescence

Robert Quast , Emmanuel Margeat
Molecular and Cellular Endocrinology, 2019, 493, pp.110469. ⟨10.1016/j.mce.2019.110469⟩
Article dans une revue hal-02346229 v1

A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription

Cédric Nadiras , Mildred Delaleau , Annie Schwartz , Emmanuel Margeat , Marc Boudvillain
Biochemistry, 2019, 58 (7), pp.865-874. ⟨10.1021/acs.biochem.8b01216⟩
Article dans une revue hal-02067416 v1
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Accurate Determination of Human CPR Conformational Equilibrium by smFRET Using Dual Orthogonal Noncanonical Amino Acid Labeling

Robert Quast , Fataneh Fatemi , Michel Kranendonk , Emmanuel Margeat , Gilles Truan
ChemBioChem, 2019, 20 (5), pp.659-666. ⟨10.1002/cbic.201800607⟩
Article dans une revue hal-02174030 v1
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Single-molecule analysis reveals the mechanism of transcription activation in M. tuberculosis

Rishi Kishore Vishwakarma , Anne-Marinette Cao , Zakia Morichaud , Ayyappasamy Sudalaiyadum Perumal , Emmanuel Margeat et al.
Science Advances , 2018, 4 (5), pp.eaao5498. ⟨10.1126/sciadv.aao5498⟩
Article dans une revue hal-02346231 v1
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Oligomerization of a G protein-coupled receptor in neurons controlled by its structural dynamics

Thor C Møller , Jérôme Hottin , Caroline Clerté , Jurriaan M. Zwier , Thierry Durroux et al.
Scientific Reports, 2018, 8 (1), ⟨10.1038/s41598-018-28682-6⟩
Article dans une revue hal-02398134 v1
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Chloride ions stabilize the glutamate-induced active state of the metabotropic glutamate receptor 3

Amélie S. Tora , Xavier Rovira , Anne-Marinette Cao , Alexandre Cabaye , Linnea Olofsson et al.
Neuropharmacology, 2018, 140, pp.275-286. ⟨10.1016/j.neuropharm.2018.08.011⟩
Article dans une revue hal-02346230 v1
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Competitive folding of RNA structures at a termination-antitermination site

Soraya Ait-Bara , Caroline Clerté , Nathalie Declerck , Emmanuel Margeat
RNA, 2017, 23 (5), pp.721-734. ⟨10.1261/rna.060178.116⟩
Article dans une revue hal-01602716 v1
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Imaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies

Mireia Ferrer , Caroline Clerté , Célia Chamontin , Eugenia Basyuk , Sébastien Lainé et al.
Nucleic Acids Research, 2016, 44 (16), pp.7922-7934. ⟨10.1093/nar/gkw511⟩
Article dans une revue hal-02141204 v1

Optimisation of vectorisation property: A comparative study for a secondary amphipathic peptide

Karidia Konate , Mattias F. Lindberg , Anaïs Vaissiere , Carole Jourdan , Gudrun Aldrian et al.
International Journal of Pharmaceutics, 2016, 509 (1-2), pp.71--84. ⟨10.1016/j.ijpharm.2016.05.030⟩
Article dans une revue hal-01878021 v1
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Automatic detection of diffusion modes within biological membranes using back-propagation neural network

Patrice Dosset , Patrice Rassam , Laurent Fernandez , Cedric Espenel , Eric Rubinstein et al.
BMC Bioinformatics, 2016, 17 (1), pp.197. ⟨10.1186/s12859-016-1064-z⟩
Article dans une revue inserm-01322561 v1
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Plasmonic Nanoantennas Enable Forbidden Förster Dipole–Dipole Energy Transfer and Enhance the FRET Efficiency

Juan de Torres , Mathieu Mivelle , Satish Babu Moparthi , Hervé Rigneault , Niek F. Van Hulst et al.
Nano Letters, 2016, 16 (10), pp.6222-6230. ⟨10.1021/acs.nanolett.6b02470⟩
Article dans une revue hal-01421637 v1
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Direct observation of the translocation mechanism of transcription termination factor Rho

V. Gocheva , Antoine Le Gall , M. Boudvillain , E. Margeat , Marcelo Nollmann
Nucleic Acids Research, 2015, 43 (4), pp.2367-2377. ⟨10.1093/nar/gkv085⟩
Article dans une revue hal-01171254 v1
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Fine tuning of sub-millisecond conformational dynamics controls metabotropic glutamate receptors agonist efficacy

Linnea Olofsson , S. Felekyan , Etienne Doumazane , Pauline Scholler , Ludovic Fabre et al.
Nature Communications, 2014, 5, pp.5206. ⟨10.1038/ncomms6206⟩
Article dans une revue hal-01943332 v1
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Chromatin insulator factors involved in long-range DNA interactions and their role in the folding of the Drosophila genome

Jutta Vogelmann , Antoine Le Gall , Stephanie Déjardin , Frédéric Allemand , Adrien Gamot et al.
PLoS Genetics, 2014, 10 (8), ⟨10.1371/journal.pgen.1004544⟩
Article dans une revue hal-02640560 v1
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The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).

Emilie Soares , Annie Schwartz , Marcelo Nollmann , Emmanuel Margeat , Marc Boudvillain
Nucleic Acids Research, 2014, 42 (14), pp.9270-9284. ⟨10.1093/nar/gku595⟩
Article dans une revue hal-01180888 v1
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Recruitment, Assembly, and Molecular Architecture of the SpoIIIE DNA Pump Revealed by Superresolution Microscopy

Jean-Bernard Fiche , Diego Cattoni , Nele Diekmann , Julio Mateos Langerak , Caroline Clerte et al.
PLoS Biology, 2013, 11 (5), pp.e1001557. ⟨10.1371/journal.pbio.1001557⟩
Article dans une revue hal-03273727 v1

Analysis of helicase-RNA interactions using nucleotide analog interference mapping.

Annie Schwartz , Makhlouf Rabhi , Emmanuel Margeat , Marc Boudvillain
Methods in Enzymology, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩
Article dans une revue hal-00726256 v1

Structural Insight into the Mycobacterium tuberculosis Rv0020c Protein and Its Interaction with the PknB Kinase

Christian Roumestand , Jade Leiba , Nathalie Galophe , Emmanuel Margeat , André Padilla et al.
Structure, 2011, 19 (10), pp.1525-1534. ⟨10.1016/j.str.2011.07.011⟩
Article dans une revue hal-03726704 v1

Mutagenesis-Based Evidence for an Asymmetric Configuration of the Ring-Shaped Transcription Termination Factor Rho

Makhlouf Rabhi , Veronika Gocheva , Frederique Jacquinot , Andrea Lee , Emmanuel Margeat et al.
Journal of Molecular Biology, 2011, 405 (2), pp.497-518. ⟨10.1016/j.jmb.2010.11.006⟩
Article dans une revue istex hal-00608241 v1

Structural Insight into the Mycobacterium tuberculosis Rv0020c Protein and Its Interaction with the PknB Kinase

Christian Roumestand , Jade Leiba , Nathalie Galophe , Emmanuel Margeat , André Padilla et al.
Structure, 2011, 19 (10), pp.1525-1534. ⟨10.1016/j.str.2011.07.011⟩
Article dans une revue hal-02282715 v1

Keeping up to speed with the transcription termination factor Rho motor.

Marc Boudvillain , Marcelo Nollmann , Emmanuel Margeat
Transcription, 2010, 1 (2), pp.70-75. ⟨10.4161/trns.1.2.12232⟩
Article dans une revue hal-00591655 v1

A stepwise 2 '-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase

Annie Schwartz , Makhlouf Rabhi , Frederique Jacquinot , Emmanuel Margeat , A. Rachid Rahmouni et al.
Nature Structural and Molecular Biology, 2009, 16 (12), pp.1309-1316. ⟨10.1038/nsmb.1711⟩
Article dans une revue istex hal-00525325 v1

Quantitative analysis of the binding of ezrin to large unilamellar vesicles containing phosphatidylinositol 4,5 bisphosphate.

Guillaume Blin , Emmanuel Margeat , Kévin Carvalho , Catherine A. Royer , Christian Roy et al.
Biophysical Journal, 2008, 94 (3), pp.1021-33. ⟨10.1529/biophysj.107.110213⟩
Article dans une revue inserm-00193998 v1

Fructose-1,6-bisphosphate acts both as an inducer and as a structural cofactor of the central glycolytic genes repressor (CggR).

Silvia Zorrilla , Denis Chaix , Alvaro Ortega , Carlos Alfonso , Thierry Doan et al.
Biochemistry, 2007, 46 (51), pp.14996-5008. ⟨10.1021/bi701805e⟩
Article dans une revue hal-00315549 v1

Inducer-modulated cooperative binding of the tetrameric CggR repressor to operator DNA.

Silvia Zorrilla , Thierry Doan , Carlos Alfonso , Emmanuel Margeat , Alvaro Ortega et al.
Biophysical Journal, 2007, 92 (9), pp.3215-27. ⟨10.1529/biophysj.106.095109⟩
Article dans une revue hal-00164236 v1
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Transcription termination factor Rho can displace streptavidin from biotinylated RNA

Annie Schwartz , Emmanuel Margeat , A. Rachid Rahmouni , Marc Boudvillain
Journal of Biological Chemistry, 2007, 282 (43), pp.31469-31476. ⟨10.1074/jbc.M706935200⟩
Article dans une revue hal-00176284 v1

The H-NS dimerization domain defines a new fold contributing to DNA recognition.

Vanessa Bloch , Yinshan Yang , Emmanuel Margeat , Alain Chavanieu , Marie Thérèse Augé et al.
Nature Structural Biology, 2003, 10 (3), pp.212-8. ⟨10.1038/nsb904⟩
Article dans une revue istex hal-00257410 v1

Structural basis for resistance of Mycobacterium tuberculosis Rho to bicyclomycin

Emmanuel Saridakis , Rishi Vishwakarma , Joséphine Lai Kee Him , Kevin Martin , Isabelle Simon et al.
Helicases & Nucleic Acid-Based Machines: Structure, Mechanism & Regulation, and Roles in HumanDisease, Jul 2021, Paris, France
Communication dans un congrès hal-03419029 v1

4D vertical imaging of plant growth in physiological conditions with two photons microscopy

Houssam Hajjoul , Marc Lartaud , Christophe Perin , Philippe Nacry , Soazig Guyomarc'H et al.
Hot Topics in Molecular Imaging-TOPIM, Jan 2014, Les Houches, France
Communication dans un congrès hal-01594253 v1

Second Harmonic Generation in GaN-based photonic crystals for single molecule investigations

Dominique Coquillat , Jeremie Torres , Marine Le Vassor d'Yerville , David Cassagne , Frederic Teppe et al.
GALLIUM NITRIDE MATERIALS AND DEVICES VII, Jan 2012, San Francisco (CA), United States. pp.82621R, ⟨10.1117/12.905593⟩
Communication dans un congrès hal-00704334 v1

Viro-fluidics: a new strategy to study HIV release in real time at the single cell and single particle scales

J. Eid , M. Socol , Antoine Naillon , J. Feuillard , Emmanuel Margeat et al.
XXIIIes Journées francophones de virologie, Apr 2021, En ligne, France
Poster de conférence hal-03424136 v1

Structural Dynamics of Single Metabotropic Glutamate Receptor Dimers

Robert B Quast , Anne-Marinette Cao , Fataneh Fatemi , Linnea Olofsson , Philippe Rondard et al.
Biophysical Society. Biophysical Society 64th Annual Meeting, Feb 2020, San Diego (CA), United States. Elsevier, BIOPHYSICAL JOURNAL, 118 (3), pp.58a, 2020, ⟨10.1016/j.bpj.2019.11.493⟩
Poster de conférence hal-04772779 v1

La viro-fluidique une nouvelle stratégie pour étudier le relargage du VIH-1

Joëlle Eid , Marius Socol , Benoît Charlot , Emmanuel Margeat , Marylène Mougel
Les journées ANRS: "Recherche dur le VIH/SIDA et les hépatites virales: quels nouveaux enjeux?", Nov 2019, Paris, France
Poster de conférence hal-02327002 v1

Relationships between Rho and RNA polymerase in living bacteria

C. Clerte , O. Manier , J-B. Fiche , M. Boudvillain , L. Bossi et al.
Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France
Poster de conférence hal-02902111 v1

Single-molecule analysis of E. coli Rho translocase, Helicase, transcription termination activities, and its interactions with RNA polymerase

R. Vishwakarma , A. Nord , F. Pedaci , M. Boudvillain , E. Margeat
Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France
Poster de conférence hal-02902115 v1