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Fernanda FONSECA

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#### **EDUCATION** **Habilitation à Diriger des Recherches (HDR).** Université de Technologie de Compiègne, 2010. Title : « Stabilization of bio functionalities trough an approach combining the understanding of mechanisms and the product-process engineering” **Ph.D. Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech),**2001**. Ph.D. thesis title: "Influence of cryoprotection and freezing on the acidification activity of thermopile lactic acid bacteria: relationships between biological and thermodynamic properties." **Master Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech), **1997**. M.Sc. thesis title: "Study of the freezing step on the activity of frozen lactic acid starters." Engineer** in **Chemical Engineering**, Montevideo University (Uruguay), **1996.** **SCIENTIFIC EXPERIENCE** ------------------------- **Research Director (since January 2015),** in Paris-Saclay Food and Bioproduct Engineering research unit (UMR 782 SayFood INRAE-AgroParisTech), Versailles-Grignon Centre, at the French National Institute for Agriculture, Food and Environment (INRAE), France. **Researcher Scientist** (**September 2001 – December 2014**), in the Joint Research Unit for Food Process Engineering and Microbiology (UMR 782 GMPA INRA-AgroParisTech), Versailles-Grignon Centre, Institut National de la Recherche Agronomique (INRA), France. **Main fields of expertise:**Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. - Bioproducts: micro-organisms, proteins, food products. - Stabilization processes: freezing and freeze-drying - Biophysical characterization of bioproducts (thermal analysis, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy) **Research approach:** Focusing on biophysical and chemical behaviors of bioproducts during stabilization processes and by relating them to biological and functional responses, to identify and quantify the mechanisms governing the degradation and preservation of bioproducts during the stabilization processes. The ambition is also to find general principles for simplifying and rationalizing the development of newly formulated products and the stabilization process optimization, with particular attention to limiting the environmental impact.

Research domains

Biotechnology Chemical and Process Engineering Engineering Sciences [physics]

Skills

Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. Bioproducts: micro-organisms, proteins, food products. Stabilization processes: freezing and freeze-drying Biophysical characterization of bioproducts (thermal analysis-DSC, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy)

Publications

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Mechanistic study of the differences in lactic acid bacteria resistance to freeze- or spray-drying and storage

Maite Gagneten , Stéphanie Passot , Stéphanie Cenard , Sarrah Ghorbal , Carolina Schebor
Applied Microbiology and Biotechnology, 2024, 108 (1), pp.361. ⟨10.1007/s00253-024-13186-3⟩
Journal articles hal-04607503v1
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Investigation of Freezing and Freeze-Drying for Preserving and Re-Using a Whole Microbial Cheese Community

Wenfan Cao , Stéphanie Passot , Françoise Irlinger , Fernanda Fonseca
Foods, 2024, 13 (12), pp.1809. ⟨10.3390/foods13121809⟩
Journal articles hal-04607514v1
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Chickpeas’ Cooking Wastewater as an Alternative Source of Galacto-Oligosaccharides for Improving the Freeze-Dried Resistance of Lactic Acid Bacteria

Stephanie Passot , Stéphanie Cenard , Pascale Lieben , Sarrah Ghorbal , Gonçalo Nuno Martins
ACS Food Science & Technology, 2024, 4 (6), pp.1490-1500. ⟨10.1021/acsfoodscitech.4c00120⟩
Journal articles hal-04607490v1
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Use of Legume Wastewater Extracts on the Storage Stability of Freeze-Dried Lactiplantibacillus plantarum WCFS1

Gonçalo Nuno Martins , María Guerrero Sánchez , Angela Daniela Carboni , Stéphanie Cenard , Fernanda Fonseca
Food and Bioprocess Technology, 2024, ⟨10.1007/s11947-024-03554-2⟩
Journal articles hal-04673277v1
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Preservation of Bacillus subtilis’ cellular liquid state at deep sub-zero temperatures in perchlorate brines

Stewart Gault , Fernanda Fonseca , Charles S Cockell
Communications Biology, 2024, 7 (1), pp.588. ⟨10.1038/s42003-024-06277-4⟩
Journal articles hal-04621468v1
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Multiobjective optimization of frozen and freeze-dried Lactobacillus delbrueckii subsp. bulgaricus CFL1 production via the modification of fermentation conditions

Maria de Lourdes Tovilla Coutino , Stephanie Passot , Ioan-Cristian Trelea , Marie-Hélène Ropers , Yann Gohon
Journal of Applied Microbiology, 2023, 134 (2), pp.1-34. ⟨10.1093/jambio/lxad003⟩
Journal articles hal-04345553v1
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Membrane lipid composition of Carnobacterium maltaromaticum CNCM I-3298, a highly cryoresistant lactic bacterium

Ha Phuong Ta , C. Clarisse , E. Maes , N. Yamakawa , Y. Guérardel
Chemistry and Physics of Lipids, 2023, 255, pp.105326. ⟨10.1016/j.chemphyslip.2023.105326⟩
Journal articles hal-04397076v1
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Insights into the mechanisms of L. salivarius CECT5713 resistance to freeze-dried storage

Maria Guerrero Sanchez , Stephanie Passot , Sarrah Ghorbal , Sonia Campoy , Monica Olivares
Cryobiology, 2023, 112, pp.104556. ⟨10.1016/j.cryobiol.2023.104556⟩
Journal articles hal-04395152v1
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Insights into lactic acid bacteria cryoresistance using FTIR micro-spectroscopy

Amélie Girardeau , Stephanie Passot , Julie Meneghel , Stéphanie Cenard , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2022, 414 (3), pp.1425-1443. ⟨10.1007/s00216-021-03774-x⟩
Journal articles hal-03552585v1
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Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

M. Guerrero Sanchez , Stephanie Passot , S. Campoy , M. Olivares , F. Fonseca
Applied Microbiology and Biotechnology, 2022, 106 (1), pp.57-80. ⟨10.1007/s00253-021-11694-0⟩
Journal articles hal-03822336v1
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Use of 3D mathematical modelling to understand the heat transfer mechanisms during freeze-drying using high-throughput vials

Juan Patricio Buceta , Stephanie Passot , Bernadette Scutellà , Erwan Bourlés , Fernanda Fonseca
Applied Thermal Engineering, 2022, 207, pp.118099. ⟨10.1016/j.applthermaleng.2022.118099⟩
Journal articles hal-03562611v1
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Effect of protective agents on the storage stability of freeze‑dried Ligilactobacillus salivarius CECT5713

Maria Guerrero Sanchez , Stéphanie Passot , Sonia Campoy , Monica Olivares , Fernanda Fonseca
Applied Microbiology and Biotechnology, 2022, 106, pp.7235-7249. ⟨10.1007/s00253-022-12201-9⟩
Journal articles hal-03845922v1
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Dynamic Modeling of Carnobacterium Maltaromaticum CNCM I-3298 Growth and Metabolite Production and Model-Based Process Optimization

Cristian Puentes , Amélie Girardeau , Stephanie Passot , Fernanda Fonseca , Ioan-Cristian Trelea
Foods, 2021, 10 (8), pp.1922. ⟨10.3390/foods10081922⟩
Journal articles hal-03552068v1
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Heat Transfer During Freeze-Drying Using a High-throughput vial System in view of Process Scale-up to Serum vials

Juan Patricio Buceta , Ioan Trelea , Bernadette Scutellà , Erwan Bourlés , Fernanda Fonseca
Journal of Pharmaceutical Sciences, 2021, 110 (3), pp.1323-1336. ⟨10.1016/j.xphs.2020.11.029⟩
Journal articles hal-03117931v1
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The transfer temperature from slow cooling to cryogenic storage is critical for optimal recovery of cryopreserved mammalian cells

Peter Kilbride , Julie Meneghel , Fernanda Fonseca , John Morris
PLoS ONE, 2021, 16 (11), pp.e0259571. ⟨10.1371/journal.pone.0259571⟩
Journal articles hal-03446319v1

FTIR micro-spectroscopy using synchrotron-based and thermal source-based radiation for probing live bacteria

Julie Meneghel , Stéphanie Passot , Frédéric Jamme , Stéphane Lefrançois , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2020, 412 (26), pp.7049-7061. ⟨10.1007/s00216-020-02835-x⟩
Journal articles hal-03004447v1
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Highlighting Protective Effect of Encapsulation on Yeast Cell Response to Dehydration Using Synchrotron Infrared Microspectroscopy at the Single-Cell Level

Thanh Dat Nguyen , Stéphane Guyot , Caroline Pénicaud , Stéphanie Passot , Christophe Sandt
Frontiers in Microbiology, 2020, 11 (6), pp.1887. ⟨10.3389/fmicb.2020.01887⟩
Journal articles hal-02939385v1
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FTIR micro-spectroscopy using synchrotron-based and thermal source-based radiation for probing live bacteria

Julie Meneghel , Stephanie Passot , Frédéric Jamme , Stéphane Lefrançois , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2020, 412 (26), pp.7049-7061. ⟨10.1007/s00216-020-02835-x⟩
Journal articles hal-03824330v1
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The Impact of varying cooling and thawing rates on the quality of cryopreserved human peripheral blood t cells

Jasmin Baboo , Peter Kilbride , Mike Delahaye , Stuart Milne , Fernanda Fonseca
Scientific Reports, 2019, 9 (1), pp.1-13. ⟨10.1038/s41598-019-39957-x⟩
Journal articles hal-02154617v1
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Influence of culture conditions on the technological properties of Carnobacterium maltaromaticum CNCM I-3298 starters

Amélie Girardeau , C. Puentes , S. Keravec , P. Peteuil , Ioan-Cristian Trelea
Journal of Applied Microbiology, 2019, 126 (5), pp.1468-1479. ⟨10.1111/jam.14223⟩
Journal articles hal-02154448v1

Écoconception des aliments, bioproduits, bioprocédés et procédés alimentaires

Caroline Pénicaud , Fernanda Fonseca
Techniques de l'Ingénieur, 2019
Journal articles hal-03086204v1
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Relating transformation process, eco-design, composition and sensory quality in cheeses using PO2 ontology

Caroline Pénicaud , Liliana Ibanescu , Thomas Allard , Fernanda Fonseca , Stephane S. Dervaux
International Dairy Journal, 2019, 92, pp.1-10. ⟨10.1016/j.idairyj.2019.01.003⟩
Journal articles hal-02043246v1
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Physical events occurring during the cryopreservation of immortalized human T cells

Julie Meneghel , Peter Kilbride , John G. Morris , Fernanda Fonseca
PLoS ONE, 2019, 14 (5), pp.e0217304. ⟨10.1371/journal.pone.0217304⟩
Journal articles hal-02154629v1
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A Guide for Ex Vivo Handling and storage of stool samples Intended for Fecal Microbiota transplantation

Sebastian D Burz , Anne-Laure Abraham , Fernanda Fonseca , Olivier David , Audrey Chapron
Scientific Reports, 2019, 9 (1), pp.8897. ⟨10.1038/s41598-019-45173-4⟩
Journal articles hal-02466120v1
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Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Fernanda Fonseca , Caroline Pénicaud , E. Elizabeth Tymczyszyn , Andrea Gómez-Zavaglia , Stephanie Passot
Applied Microbiology and Biotechnology, 2019, 103 (17), pp.6867-6883. ⟨10.1007/s00253-019-10002-1⟩
Journal articles hal-03824544v1
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Determination of the dried product resistance variability and its influence on the product temperature in pharmaceutical freeze-drying

Bernadette Scutella , Ioan-Cristian Trelea , Erwan Bourlés , Fernanda Fonseca , Stéphanie Passot
European Journal of Pharmaceutics and Biopharmaceutics, 2018, 128, pp.379-388. ⟨10.1016/j.ejpb.2018.05.004⟩
Journal articles hal-01789065v1
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Effect of freeze-dryer design on heat transfer variability investigated using a 3D mathematical model

B. Scutellà , E. Bourlés , Artemio Plana-Fattori , F. Fonseca , D. Flick
Journal of Pharmaceutical Sciences, 2018, 107 (8), pp.2098-2106. ⟨10.1016/j.xphs.2018.04.002⟩
Journal articles hal-01769104v1

Life cycle assessment of the production of stabilized lactic acid bacteria for the environmentally-friendly preservation of living cells

Caroline Pénicaud , Vincent Monclus , Bruno Perret , Stéphanie Passot , Fernanda Fonseca
Journal of Cleaner Production, 2018, 184, pp.847-858. ⟨10.1016/j.jclepro.2018.02.191⟩
Journal articles hal-01784901v1

Nouveaux dispositifs médicaux et nouveau procédé de fabrication pour un transfert de microbiote fécal par voie rectale, sans risque, standardisé et prêt à l’emploi

Jean-François Dubuisson , Catherine Juste , Fernanda Fonseca , Patricia Lepage , Christel Béra-Maillet
Journal de Pharmacie Clinique, 2018, 37 (2), pp.73-83
Journal articles hal-02628188v1
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The virtual food system: Innovative models and experiential feedback in technologies for winemaking, the cereals chain, food packaging and eco-designed starter production

Cesar Arturo Aceves-Lara , Violaine Athès , Patrice Buche , Guy Della Valle , Vincent Farines
Innovative Food Science & Emerging Technologies / Innovative Food Science and Emerging Technologies , 2018, Food Science and Technology in France: INRA’s contribution to this area, 46, pp.54-64. ⟨10.1016/j.ifset.2017.10.006⟩
Journal articles lirmm-01651998v1
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How vial geometry variability influences heat transfer and product temperature during freeze-drying

Bernadette Scutella , Stéphanie Passot , Erwan Bourlés , Fernanda Fonseca , Ioan-Cristian Trelea
Journal of Pharmaceutical Sciences, 2017, 106 (3), pp.770-778. ⟨10.1016/j.xphs.2016.11.007⟩
Journal articles hal-01465155v1

Subcellular membrane fluidity of Lactobacillus delbrueckii subsp. bulgaricus under cold and osmotic stress

Julie Meneghel , Stéphanie Passot , Stéphanie Cenard , Matthieu Refregiers , Frédéric Jamme
Applied Microbiology and Biotechnology, 2017, 101 (18), pp.6907 - 6917. ⟨10.1007/s00253-017-8444-9⟩
Journal articles hal-01634459v1

Biophysical characterization of the Lactobacillus delbrueckii subsp. bulgaricus membrane during cold and osmotic stress and its relevance for cryopreservation

Julie Meneghel , Stéphanie Passot , Sébastien Dupont , Fernanda Fonseca
Applied Microbiology and Biotechnology, 2017, 101 (4), pp.1427 - 1441. ⟨10.1007/s00253-016-7935-4⟩
Journal articles hal-01559240v1

Understanding the responses of Saccharomyces cerevisiae yeast strain during dehydration processes using synchrotron infrared spectroscopy

T. Nguyen , Stéphane Guyot , Caroline Pénicaud , S. Passot , C. Sandt
Analyst, 2017, 142 (19), pp.3620 - 3628. ⟨10.1039/c7an00257b⟩
Journal articles hal-01616052v1
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3D mathematical modelling to understand atypical heat transfer observed in vial freeze-drying

Bernadette Scutellà , Artemio Plana-Fattori , Stéphanie Passot , E Bourlès , Fernanda Fonseca
Applied Thermal Engineering, 2017, 126, pp.226 - 236. ⟨10.1016/j.applthermaleng.2017.07.096⟩
Journal articles hal-01582545v1
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Draft Genome Sequence of Lactobacillus delbrueckii subsp. bulgaricus CFL1, a Lactic Acid Bacterium Isolated from French Handcrafted Fermented Milk

Julie Meneghel , Eric Dugat-Bony , Françoise Irlinger , Valentin Loux , Marie Vidal
Genome Announcements, 2016, 4 (2), pp.e00052-16. ⟨10.1128/genomea.00052-16⟩
Journal articles hal-03649172v1
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Dynamic modelling of the secondary drying stage of freeze-drying reveals distinct desorption kinetics for bound water

Ioan-Cristian Trelea , Fernanda Fonseca , Stéphanie Passot
Drying Technology, 2016, 34 (3), pp.335-345. ⟨10.1080/07373937.2015.1054509⟩
Journal articles hal-01531598v1

Protection of yeast cells in micro-organized shells of natural polyelectrolytes during drying process

Thanh Dat Nguyen , Stéphane Guyot , Christophe Sandt , Caroline Pénicaud , Stéphanie Passot
STP Pharma Pratiques, 2016, 26 (2), pp.133
Journal articles hal-01531646v1
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Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation

Fernanda Fonseca , Julie Meneghel , Stéphanie Cenard , Stéphanie Passot , G. John Morris
PLoS ONE, 2016, 11 (4), pp.e0152939. ⟨10.1371/journal.pone.0152939⟩
Journal articles hal-01565156v1
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Draft Genome Sequence of Lactobacillus delbrueckii subsp. bulgaricus CFL1, a Lactic Acid Bacterium Isolated from French Handcrafted Fermented Milk

Julie Meneghel , Eric Dugat-Bony , Françoise Irlinger , Valentin Loux , Marie Vidal
Genome Announcements, 2016, 4 (2), pp.e00052-16. ⟨10.1128/genomeA.00052-16⟩
Journal articles hal-01511494v1

Cyclopropanation of unsaturated fatty acids and membrane rigidification improve the freeze-drying resistance of Lactococcus lactis subsp lactis TOMSC161

Helene Velly , Marielle Bouix , Stéphanie Passot , Caroline Pénicaud , Helene Beinsteiner
Applied Microbiology and Biotechnology, 2015, 99 (2), pp.907 - 918. ⟨10.1007/s00253-014-6152-2⟩
Journal articles hal-01535230v1
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A binary gas transport model Improves the prediction of mass transfer in freeze drying

Ioan-Cristian Trelea , Fernanda Fonseca , Stéphanie Passot , Denis Flick
Drying Technology, 2015, 33 (15-16), pp.1849-1858. ⟨10.1080/07373937.2015.1040025⟩
Journal articles hal-01269386v1

Understanding the cryotolerance of lactic acid bacteria using combined synchrotron infrared and fluorescence microscopies

Stéphanie Passot , Julie Gautier , Frédéric Jamme , Stéphanie Cenard , Paul Dumas
Analyst, 2015, 140 (17), pp.5920-5928. ⟨10.1039/C5AN00654F⟩
Journal articles hal-01536496v1
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Genome Sequence of the Lactic Acid Bacterium Lactococcus lactis subsp. lactis TOMSC161, Isolated from a Nonscalded Curd Pressed Cheese

Hélène Velly , Pierre Renault , Anne-Laure Abraham , Valentin Loux , Agnes Delacroix-Buchet
Genome Announcements, 2014, 2 (6), ⟨10.1128/genomeA.01121-14⟩
Journal articles hal-01193999v1
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Physiological and biochemical responses of Yarrowia lipolytica to dehydration induced by air-drying and freezing

Caroline Pénicaud , Sophie Landaud-Liautaud , Frederic Jamme , Pauline Talbot , Marielle Bouix
PLoS ONE, 2014, 9 (10), ⟨10.1371/journal.pone.0111138⟩
Journal articles hal-01195500v1

Cell growth and resistance of Lactococcus lactis subsp lactis TOMSC161 following freezing, drying and freeze-dried storage are differentially affected by fermentation conditions

Helene Velly , Fernanda Fonseca , Stéphanie Passot , Agnes Delacroix-Buchet , Marielle Bouix
Journal of Applied Microbiology, 2014, 117 (3), pp.729 - 740. ⟨10.1111/jam.12577⟩
Journal articles hal-01194098v1

Synchrotron UV fluorescence microscopy for determining membrane fluidity modification of single bacteria with temperatures

Stéphanie S. Passot , Frederic Jamme , M. Refregiers , Julie J. Gautier , Stéphanie Cenard
Biomedical Spectroscopy and Imaging, 2014, 3 (3), pp.203-210. ⟨10.3233/BSI-140062⟩
Journal articles hal-02629450v1

A low membrane lipid phase transition temperature is associated with a high cryotolerance of [i]Lactobacillus delbrueckii[/i] subspecies [i]bulgaricus[/i] CFL1

Julie J. Gautier , Stéphanie S. Passot , Caroline Pénicaud , Herve H. Guillemin , Stéphanie S. Cenard
Journal of Dairy Science, 2013, 96 (9), pp.5591-5602. ⟨10.3168/jds.2013-6802⟩
Journal articles hal-01001061v1
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A low temperature limit for life on Earth

Andrew Clarke , G. John Morris , Fernanda Fonseca , Benjamin J. Murray , Elizabeth Acton
PLoS ONE, 2013, 8 (6), ⟨10.1371/journal.pone.0066207⟩
Journal articles hal-01190603v1

A low temperature limit for life on earth

A. Clark , G.J. Morris , F. Fonseca , B.J. Murray , E. Acton
PLoS ONE, 2013, in press
Journal articles hal-01564482v1

Freezing injury: the special case of the sperm cell

G.J. Morris , E. Acton , B. Murray , F. Fonseca
Cryobiology, 2012, 64, pp.71--80
Journal articles hal-01564145v1
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Critical water activity and amorphous state for optimal preservation of lyophilised lactic acid bacteria

Stéphanie Passot , Stéphanie Cenard , Inès Douania , Ioan Cristian Trelea , Fernanda Fonseca
Food Chemistry, 2012, 132 (4), pp.1699-1705. ⟨10.1016/j.foodchem.2011.06.012⟩
Journal articles hal-01536691v1

Freezing injury: The special case of the sperm cell

G. John Morris , Elizabeth Acton , Benjamin J. Murray , Fernanda Fonseca
Cryobiology, 2012, 64 (2), pp.71-80. ⟨10.1016/j.cryobiol.2011.12.002⟩
Journal articles hal-02649267v1
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Toward Optimal Operation Conditions of Freeze-Drying Processes via a Multilevel Approach

Luis T. Antelo , Stéphanie Passot , Fernanda Fonseca , Ioan-Cristian Trelea , Antonio A. Alonso
Drying Technology, 2012, 30 (13), pp.1432 - 1448. ⟨10.1080/07373937.2012.686079⟩
Journal articles hal-01053126v1

Freezing protocol effect on quality properties of fruit tissue according to the fruit, the variety and the stage of maturity

Sophie Chassagne-Berces , Fernanda Fonseca , Morgane Citeau , Michele Marin
LWT - Food Science and Technology, 2010, 43 (9), pp.1441 - 1449. ⟨10.1016/j.lwt.2010.04.004⟩
Journal articles hal-01195463v1

Effect of controlled ice nucleation on primary drying stage and protein recovery in vials cooled in a modified freeze-dryer

Stéphanie Passot , Ioan-Cristian Trelea , Michele Marin , Miquel Galan , G. John Morris
Journal of Biomechanical Engineering, 2009, 131 (7), pp.074511:1-5. ⟨10.1115/1.3143034⟩
Journal articles hal-01537095v1
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Model for Heat and Mass Transfer in Freeze-Drying of Pellets

Ioan-Cristian Trelea , Stephanie Passot , Michele Marin , Fernanda Fonseca
Journal of Biomechanical Engineering, 2009, 131 (7), pp.074501. ⟨10.1115/1.3142975⟩
Journal articles hal-01536947v1

Changes in texture, cellular structure and cell wall composition in apple tissue as a result of freezing

Sophie Chassagne , Cécile Poirier , Marie Francoise Devaux , Fernanda Fonseca , Marc Lahaye
Food Research International, 2009, 42 (7), pp.788-797. ⟨10.1016/j.foodres.2009.03.001⟩
Journal articles hal-01195428v1
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An Interactive Tool for the Optimization of Freeze-Drying Cycles Based on Quality Criteria

Ioan-Cristian Trelea , Stéphanie Passot , Fernanda Fonseca , Michele Marin
Drying Technology, 2007, 25 (5), pp.741-751. ⟨10.1080/07373930701370100⟩
Journal articles hal-01537101v1

Rapidly cooled horse spermatozoa: loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation

G. John Morris , K. Faszer , J. E. Green , D. Draper , B. W. W. Grout
Theriogenology, 2007, 68 (5), pp.804-812
Journal articles hal-02653848v1

Effect of product temperature applied during primary drying on the long-term stability of lyophilized proteins

Stéphanie S. Passot , Fernanda Fonseca , Naziha Barbouche , - M. Alarcon-Lorca , - D. Rolland
Pharmaceutical Development and Technology, 2007, 12 (6), pp.543-53
Journal articles hal-02655952v1

Stabilization of frozen Lactobacillus delbrueckii subsp. bulgaricus in glycerol suspensions : freezing kinetics and storage temperature effects

Fernanda Fonseca , Michèle Marin , G. J. Morris
Applied and Environmental Microbiology, 2006, 72 (10), pp.6474-6482. ⟨10.1128/aem.00998-06⟩
Journal articles hal-02663860v1

The high viscosity encountered during freezing in glycerol solutions: effects on cryopreservation

G. John Morris , Martha Goodrich , Elizabeth Acton , Fernanda Fonseca
Cryobiology, 2006, 52 (3), pp.323-334. ⟨10.1016/j.cryobiol.2006.01.003⟩
Journal articles hal-02667959v1

Caractérisation et lyophilisation de matrices fromagères

Catherine Béal , Fernanda Fonseca , A. Thomas , Michèle Marin
Sciences des aliments = Food science : an international journal of food science and technology, 2006, 26 (1), pp.89-102. ⟨10.3166/sda.26.89-102⟩
Journal articles hal-02653766v1

Physical characterisation of formulations for the development of two stable freeze-dried proteins during both dried and liquid storage

Stéphanie S. Passot , Fernanda Fonseca , M. Alarcon-Lorca , D. Rolland , Michèle Marin
European Journal of Pharmaceutics and Biopharmaceutics, 2005, 60 (3), pp.335-348. ⟨10.1016/j.ejpb.2005.02.013⟩
Journal articles hal-02679545v1

Effect of sucrose and maltodextrin on the physical properties and survival of air-dried Lactobacillus bulgaricus: an in situ fourier transform infrared spectroscopy study

Harriëtte Oldenhof , Willem F. Wolkers , Fernanda Fonseca , Stéphanie S. Passot , Michèle Marin
Biotechnology Progress, 2005, 21 (3), pp.885-892. ⟨10.1021/bp049559j⟩
Journal articles hal-02680835v1

Collapse temperature of bacterial suspensions: the effect of cell type and concentration

Fernanda Fonseca , Stéphanie S. Passot , Pascale Lieben , Michèle Marin
Cryo Letters, 2004, 25 (4), pp.425-434
Journal articles hal-02672062v1

Collapse temperature of freeze-dried Lactobacillus bulgaricus suspensions and protective media

Fernanda Fonseca , Stéphanie S. Passot , O. Cunin , Michèle Marin
Biotechnology Progress, 2004, 20 (1), pp.229-238. ⟨10.1021/bp034136n⟩
Journal articles hal-02679282v1

Improvement of cryopreservation of Lactobacillus delbrueckii subsp. bulgaricus CFL1 with additives displaying different protective effects

Fernanda Fonseca , Catherine Béal , Fatma Mihoub , Michèle Marin , Georges G. Corrieu
International Dairy Journal, 2003, 13 (11), pp.917-926. ⟨10.1016/S0958-6946(03)00119-5⟩
Journal articles hal-02680827v1

Improvement of cryopreservation of Lactobacillus delbruekii subsp bulgaricus CFL1 with additives displaying different protective effects

Fernanda Fonseca , Catherine Béal , Fatma Mihoub , Michèle Marin , Georges Corrieu
International Dairy Journal, 2003, 13 (11), pp.917-926
Journal articles hal-02673309v1

State diagrams and sorption isotherms of bacterial suspensions and fermented medium

Fernanda Fonseca , Jean-Philippe Obert , Catherine Béal , Michèle Marin
Thermochimica Acta, 2001, 366 (2), pp.167-182. ⟨10.1016/S0040-6031(00)00725-5⟩
Journal articles hal-02678486v1

Operating conditions that affect the resistance of lactic acid bacteria to freezing and frozen storage

Fernanda Fonseca , Catherine Béal , Georges Corrieu
Cryobiology, 2001, 43 (3), pp.189-198. ⟨10.1006/cryo.2001.2343⟩
Journal articles hal-02681116v1

Resistance to freezing and frozen storage of Streptococcus thermophilus is related to membrane fatty acid composition

Catherine Béal , Fernanda Fonseca , Georges Corrieu
Journal of Dairy Science, 2001, 84 (11), pp.2347-2356
Journal articles hal-02670204v1
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Resistance to freezing and to frozen storage of Streptococcus themophilus is related to membrane fatty acid composition

Catherine Béal , Fernanda Fonseca , Georges G. Corrieu
Journal of Dairy Science, 2001, 84 (11), pp.2347-2356
Journal articles hal-02670974v1

Method of quantifying the loss of acidification activity of lactic acid starters during freezing and frozen storage

Fernanda Fonseca , Catherine Béal , Georges Corrieu
Journal of Dairy Research, 2000, 67 (1), pp.83-90
Journal articles hal-02696403v1
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The challenge of preserving beneficial bacteria: strategies and tools to identify stress mechanisms and sites of cell damage induced by dehydration processes

Fernanda Fonseca , Stephanie Passot
ISLFD 2024 - 10Th International Symposium on Lyophilization of Pharmaceuticals, May 2024, Turin (IT), Italy
Conference papers hal-04647619v1

Differential scanning calorimetry applied to the preservation of bacteria and mammalian cells

Fernanda Fonseca , Stéphanie Passot
JCAT53 - 53èmes Journées de Calorimétrie et d'Analyse Thermique, May 2023, Palaiseau, France
Conference papers hal-04395225v1

Environmental performances of the production of dried lactic acid bacteria concentrates protected by fructo-oligosaccharides ICEF14, June 20 th to 23 rd 2023, Nantes, France

Stéphanie Passot , Maite Gagneten , Camille Quentier , Stéphanie Cenard , Baptiste Gauducheau-Chevalier
International Congress on Engineering and Food, Jun 2023, Nantes, France
Conference papers hal-04397246v1

Perchlorate salts lower the vitrification point of Bacillus subtilis to temperatures lower than those expected beneath the Martian south pole

Stewart Gault , Charles S. Cockell , Fernanda Fonseca , Stephanie Passot
EANA - European Astrobiology Network Association, Universidad Carlos III de Madrid Ronda de Toledo, Sep 2023, Madrid, Spain
Conference papers hal-04564015v1

Stabilization of Lactiplantibacillus plantarum WCFS1 during freezing, freeze-drying, and storage using legume wastewater extracts containing galacto-oligosaccharides

Gonçalo N. Martins , Maria Guerrero Sanchez , Angela D. Carboni , Fernanda Fonseca , Sonia Campoy
10th CQM Annual Meeting, Jun 2023, Funchal, Portugal
Conference papers hal-04397545v1

Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole

Stewart Gault , Charles S Cockell , Fernanda Fonseca , Stéphanie Passot
European Astrobiology Network Association, Sep 2023, Madrid, Spain
Conference papers hal-04397603v1

A critical transfer temperature from slow cooling to cryogenic storage for optimal recovery across a range of cryopreserved mammalian cells.

Julie Meneghel , Peter Kilbride , Fernanda Fonseca , John Morris
Annual Meeting of the Society for Cryobiology, Jul 2022, Dublin, Ireland
Conference papers hal-04397788v1

Perchlorate brines in the Martian deep subsurface and the low temperature limit for life

Stewart Gault , Charles S. Cockell , Fernanda Fonseca , Stéphanie Passot
Astrobiology Science Conference, May 2022, Atlanta, United States
Conference papers hal-04397708v1

Perchlorate brines in the Martian deep subsurface and the low temperature limit for life.

Stewart Gault , Charles S. Cockell , Fernanda Fonseca , Stephanie Passot
Astrobiology Science Conference, 2022, Atlanta, United States
Conference papers hal-04564033v1

Bacterial cell adaptation to environmental stress during fermentation improves cryo-and drying resistance: A focus on membrane properties

Maria de L Tovilla-Coutino , Stéphanie Passot , Jérôme Delettre , Sarrah Ghorbal , Marie-Hélène Ropers
5th Microbial Stress Conference of the European Federation of Biotechnology, Nov 2020, Virtual, France
Conference papers hal-04398166v1

Effects of pH stress during growth on the physiological, biophysical and biochemical properties of Carnobacterium maltaromaticum cells

Amélie Girardeau , Stéphanie Passot , Stéphanie Cénard , Pascale Lieben , Fernanda Fonseca
5th Microbial Stress Conference of the European Federation of Biotechnology, Nov 2020, Virtual, France
Conference papers hal-04398133v1

Fructose oligosaccharides as a novel cryoprotectant for mammalian cells

Peter Kilbride , Hugo, A , Julie Meneghel , Jérôme J. Delettre , Romano, N
59th Annual Meeting of the Society for Cryobiology, Jul 2020, Inconnue, France. pp.174
Conference papers hal-03006765v1

Avancées récentes sur la production et la stabilisation de bactéries lactiques à haute fonctionnalité

Stéphanie Passot , Catherine Béal , Fernanda Fonseca
CBL 2019 - 22. Colloque du Club des Bactéries Lactiques, Jun 2019, Caen, France
Conference papers hal-02268915v1
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Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation

Fernanda Fonseca , Julie Meneghel , Peter Kilbride , Stéphanie Passot , G. John Morris
9. International Symposium on Lyophilization of Pharmaceuticals ISLFD, Sep 2019, Ghent, Belgium
Conference papers hal-02734376v1

Complementary analytical approaches improving knowledge on lactic acid bacteria cryoresistance

Amélie Girardeau , Julie Meneghel , Ioan-Cristian Trelea , Stéphanie Passot , Fernanda Fonseca
56. Annual Meeting of the Society for Cryobiology, Jul 2019, San Diego, CA, United States. 166-166 p., ⟨10.1016/j.cryobiol.2019.10.086⟩
Conference papers hal-02266910v1

Development of a simplified lipid model of bacterial membrane to investigate specific interactions with oligosaccharides

Fernanda Fonseca , Michele da Silva Pinto , Julie Meneghel , Pascale Lieben , Stéphanie Passot
56. Annual Meeting of the Society for Cryobiology, Jul 2019, San Diego, CA, United States. pp.173, ⟨10.1016/j.cryobiol.2019.10.109⟩
Conference papers hal-02266911v1

Methodological approach towards environmentally friendly processes for preserving lactic acid bacteria

Caroline Pénicaud , Bruno Perret , Stéphanie Passot , Camille Quentier , Fernanda Fonseca
Club des Bactéries Lactiques, Jun 2019, Caen, France
Conference papers hal-02266899v1

Recent advances on production and stabilization of lactic acid bacteria with high de functionality

Stéphanie Passot , Catherine Béal , Fernanda Fonseca
CBL 2019 - 22. Colloque du Club des Bactéries Lactiques, Jun 2019, Caen, France
Conference papers hal-02998766v1

The critical thermal range for controlled rate cooling for mammalian cell cryopreservation

Peter Kilbride , Julie Meneghel , Fernanda Fonseca , John Morris
56. Annual Meeting of the Society for Cryobiology, Jul 2019, San Diego, United States. ⟨10.1016/j.cryobiol.2019.10.118⟩
Conference papers hal-02266912v1

LIDeOGraM: an interactive evolutionary modelling tool

Thomas Chabin , Marc Barnabe , Nadia Boukhelifa , Fernanda Fonseca , Alberto Tonda
13. Biennial International Conference on Artificial Evolution (EA), 2018, CHAM, Switzerland. pp.13, ⟨10.1007/978-3-319-78133-4_14⟩
Conference papers hal-02736924v1

A semi-automatic modelling approach for the production and freeze drying of lactic acid bacteria

Thomas Chabin , Marc Barnabe , Alberto Tonda , Nadia Boukhelifa , Fernanda Fonseca
10. International Conference on Simulation and Modelling in the Food and Bio-Industry, FOODSIM 2018. Eurosis, 2018., Apr 2018, Ghent, Belgium
Conference papers hal-02266291v1

Use of a multi-vial mathematical model to design freeze-drying cycles for pharmaceuticals at known risk of failure

Bernadette Scutellà , Ioan-Cristian Trelea , Erwan Bourlés , Fernanda Fonseca , Stéphanie Passot
21. International Drying Symposium, Sep 2018, Valencia, Spain. ⟨10.4995/IDS2018.2018.7421⟩
Conference papers hal-02266914v1
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Interactive evolutionary modelling of living complex food systems

Thomas Chabin , Marc Barnabe , Nadia Boukhelifa , Fernanda Fonseca , Alberto Tonda
Genetic and Evolutionary Computation Conference GECCO' 17, Jul 2017, Berlin, Germany. ⟨10.1145/3067695.3075992⟩
Conference papers hal-01607872v1

Les bactéries lactiques sous la lumière du synchrotron pour comprendre les mécanismes de cryotolérance

Stéphanie Passot , Frederic Jamme , Mathieu Réfrégiers , Julie Meneghel , Paul Dumas
Société Française de Microbiologie, Oct 2017, Paris, France
Conference papers hal-02266902v1
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Eco-conception des bio‐procédés : exemple des bactéries lactiques stabilisées

Caroline Pénicaud , Fernanda Fonseca , Bruno Perret , Stephanie Passot , Ioan-Cristian Trelea
Métaséminaire CEPIA 2017, INRA-CEPIA, Mar 2017, Massy, France
Conference papers hal-04644051v1
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LIDeOGraM : an interactive evolutionary modelling tool

Thomas Chabin , Marc Barnabe , Nadia Boukhelifa , Fernanda Fonseca , Alberto Tonda
Biennial International Conference on Artificial Evolution (EA-2017), 2017, Paris, France
Conference papers hal-01607681v1

Stabilisation d'écosystèmes microbiens fromagers : preuve de concept

Francoise Irlinger , Wenfan Cao , Thomas Cattenoz , Stéphanie Passot , Marie Noelle Leclercq-Perlat
Société Française de Microbiologie, Oct 2017, Paris, France
Conference papers hal-02266905v1

Can the desorption kinetics explain the residual moisture content heterogeneity observed in pharmaceuticals freeze-drying process?

Bernadette Scutella , Erwan Bourlés , Cassandre Tordjman , Fernanda Fonseca , Yves Mayeresse
6. European Drying Conference: EuroDrying 2017, Jun 2017, Liège, Belgium
Conference papers hal-01604833v1

Eco-design of food and bio-processes: Example of production of stabilized lactic acid bacteria

Caroline Pénicaud , Vincent Monclus , Bruno Perret , Stéphanie Passot , Ioan-Cristian Trelea
Lecture at WUR, Sep 2016, Wageningen, Netherlands
Conference papers hal-01532620v1
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Finite elements model of heat transfer predicts the edge vial effect in different freeze-drying configurations

Bernadette Scutella , Stéphanie Passot , Erwan Bourlés , Fernanda Fonseca , Artemio Plana-Fattori
2016 CPPR Conference Freeze-Drying of Pharmaceuticals and Biologicals, Jul 2016, Breckenridge, United States
Conference papers hal-01567200v1

Production of stabilized lactic acid bacteria viewed from a life cycle assessment perspective

Caroline Pénicaud , Vincent Monclus , Bruno Perret , Stéphanie Passot , Fernanda Fonseca
LCA Food 2016, Oct 2016, Dublin, Ireland
Conference papers hal-01532621v1
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Freeze-drying control by taking into account environmental considerations (communication orale)

Caroline Pénicaud , Ioan Cristian Tréléa , Bruno Perret , Fernanda Fonseca , Stéphanie Passot
FoodFactory, Oct 2016, Laval, France
Conference papers hal-01589685v1
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Mapping membrane fluidity in single bacterial cells to study the impact of cryopreservation and its related stresses

Julie Meneghel , Stéphanie Passot , Stéphanie Cenard , Matthieu Refregiers , Frédéric Jamme
2. International Conference on Physics and Biological Systems 2016, Oct 2016, Gif-sur-Yvette, France
Conference papers hal-02268917v1

How to manage scale-up using the design space approach

Bernadette Scutella , Erwan Bourlés , Fernanda Fonseca , Artemio Plana-Fattori , Denis Flick
2016 PDA Conference, Pharmaceutical Freeze Drying Technology, PDA., Sep 2016, Strasbourg, France
Conference papers hal-01567201v1

Initial measurement of the intracellular glass transition of a mammalian cell type under cryopreservation conditions

Fernanda Fonseca , Julie Meneghel , Stéphanie Cenard , Stéphanie Passot , D. Gilham
52nd Annual Meeting of the Society for Crryobiology, Jul 2015, Ostrava, Czech Republic. pp.538, ⟨10.1016/j.cryobiol.2015.10.008⟩
Conference papers hal-01535362v1

Environmentally eco-reasoned design process for functional yeast production and stabilization

Antonio Camarasa Junior , Thanh Dat Nguyen , Hélène Simonin , Rémi Saurel , Anne Endrizzi
3. European Congress of Applied Biotechnology (ECAB 3), Sep 2015, Nice, France
Conference papers hal-02266908v1

Life cycle assessment for an eco-friendly management of the production of stabilized microorganisms

Vincent Monclus , Caroline Pénicaud , Bruno Perret , Stéphanie Passot , Fernanda Fonseca
Life Cycle Management 2015, Aug 2015, Bordeaux, France
Conference papers hal-01536576v1

Dynamic characterization of membrane biophysical properties to better understand bacterial tolerance to osmotic and cold stresses encountered during freezing

Julie Meneghel , Stéphanie Passot , Frederic Jamme ,