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Mélanie Paillard

Assistant Professor/CRCN Inserm
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Présentation

Mélanie Paillard is an Inserm Associate Researcher in the IRIS (Ischemia-Reperfusion Injury Syndromes) team at CarMeN laboratory in Lyon. Her main research focus consists in pursuing a translational research for the development of new protective strategies targeting mitochondria during cardiometabolic diseases (including diabetic cardiomyopathy, HFpEF, myocardial infarction and stroke). Mélanie graduated from the Ecole Normale Supérieure of Lyon in 2008 and obtained her PhD in Physiology in 2012 from the University Claude Bernard Lyon1. Her PhD work focused on the role of some mitochondrial functions in the mechanisms of cardiomyocyte death during ischemia-reperfusion, notably the reticulum-mitochondria Ca2+ coupling (Paillard et al., Circulation 2013). Mélanie joined the MitoCare center of Pr Gyorgy Hajnoczky in 2013 (Philadelphia, USA), supported by postdoctoral fellowships from Fondation pour la Recherche Médicale and the American Heart Association. During her post-doctoral training, her research led to the discovery of the physio-pathological role of MICU1, a key regulator of the mitochondrial uniporter (Antony, Paillard et al., Nat Commun 2016; Paillard et al., Mol Cell 2018), for which she received the Young Bioenergeticist Award in 2017. Thanks to the combination of state-of-the-art techniques in live fluorescent imaging microscopy, proteomic analyses and in vivo mouse models up to the translational level in isolated human cardiomyocytes, she is now conducting a translational research focusing on mitochondrial Ca2+ regulation in the excitation-metabolic coupling, notably in diabetic HFpEF (Dia et al., Basic Res Cardiol 2020) and myocardial infarction (ANR MitoCaRe).

Domaines de recherche


Compétences

Publications

MICU1 controls the sensitivity of the mitochondrial Ca2+ uniporter to activators and inhibitors

Macarena Rodríguez-Prados , Kai-Ting Huang , Katalin Márta , Melanie Paillard , György Csordás
Cell Chemical Biology, 2023, 30 (6), pp.606-617.e4. ⟨10.1016/j.chembiol.2023.05.002⟩
Article dans une revue hal-04316205v1

SERCA2 phosphorylation at serine 663 is a key regulator of Ca2+ homeostasis in heart diseases

Fabrice Gonnot , Laura Boulogne , Camille Brun , Maya Dia , Yves Gouriou
Nature Communications, 2023, 14 (1), pp.3346. ⟨10.1038/s41467-023-39027-x⟩
Article dans une revue hal-04316213v1
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Long COVID and the cardiovascular system - elucidating causes and cellular mechanisms in order to develop targeted diagnostic and therapeutic strategies: A joint Scientific Statement of the ESC Working Groups on Cellular Biology of the Heart and Myocardial & Pericardial Diseases

M. Gyöngyösi , P. Alcaide , F. W. Asselbergs , Bjjm Brundel , G. G. Camici
Cardiovascular Research, 2023, 119 (2), pp.336-356. ⟨10.1093/cvr/cvac115⟩
Article dans une revue inserm-03831076v1

MICU1 occludes the mitochondrial calcium uniporter in divalent-free conditions

Macarena Rodríguez-Prados , Elena Berezhnaya , Maria Teresa Castromonte , Sergio Menezes-Filho , Melanie Paillard
Proceedings of the National Academy of Sciences of the United States of America, 2023, 120 (19), ⟨10.1073/pnas.2218999120⟩
Article dans une revue hal-04316217v1
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A bibliometric analysis: Ca2+ fluxes and inflammatory phenotyping by flow cytometry in peripheral blood mononuclear cells

Camille Brun , Lucie Chalet , Florentin Moulin , Thomas Bochaton , Sylvie Ducreux
Frontiers in Immunology, 2023, 14, pp.1272809. ⟨10.3389/fimmu.2023.1272809⟩
Article dans une revue hal-04244675v1
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Effect of Metformin on T2D-Induced MAM Ca2+ Uncoupling and Contractile Dysfunction in an Early Mouse Model of Diabetic HFpEF

Maya Dia , Christelle Leon , Stéphanie Chanon , Nadia Bendridi , Ludovic Gomez
International Journal of Molecular Sciences, 2022, 23 (7), pp.3569. ⟨10.3390/ijms23073569⟩
Article dans une revue hal-03651077v1

Altered composition of the mitochondrial Ca2+uniporter in the failing human heart

Melanie Paillard , Kai-Ting Huang , David Weaver , Jonathan Lambert , John Elrod
Cell Calcium, 2022, 105, pp.102618. ⟨10.1016/j.ceca.2022.102618⟩
Article dans une revue hal-03789636v1
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Endoplasmic reticulum-mitochondria miscommunication is an early and causal trigger of hepatic insulin resistance and steatosis

A. Beaulant , M. Dia , B. Pillot , M. A. Chauvin , J. Ji-Cao
Journal of Hepatology, 2022, 77 (3), pp.710-722. ⟨10.1016/j.jhep.2022.03.017⟩
Article dans une revue inserm-03754177v1
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Uncontrolled mitochondrial calcium uptake underlies the pathogenesis of neurodegeneration in MICU1-deficient mice and patients

R. Singh , A. Bartok , M. Paillard , A. Tyburski , M. Elliott
Science Advances , 2022, 8 (11), pp.eabj4716. ⟨10.1126/sciadv.abj4716⟩
Article dans une revue inserm-03754195v1

Myocardial Ischemia-Reperfusion and Diabetes: Lessons Learned From Bedside to Bench

Maya Dia , Alexandre Paccalet , Bruno Pillot , Christelle Leon , Michel Ovize
Frontiers in Cardiovascular Medicine, 2021, 8, ⟨10.3389/fcvm.2021.660698⟩
Article dans une revue hal-03299392v1
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Pharmacological inhibition of the mitochondrial Ca(2+) uniporter: Relevance for pathophysiology and human therapy

K. Márta , P. Hasan , M. Rodríguez-Prados , M. Paillard , G. Hajnóczky
Journal of Molecular and Cellular Cardiology, 2021, 151, pp.135-144. ⟨10.1016/j.yjmcc.2020.09.014⟩
Article dans une revue inserm-03261172v1

An innovative sequence of hypoxia-reoxygenation on adult mouse cardiomyocytes in suspension to perform multilabeling analysis by flow cytometry

A. Paccalet , N. Tessier , M. Paillard , L. Paita , L. Gomez
American journal of physiology. Cell physiology, 2020, 318 (2), pp.C439-C447. ⟨10.1152/ajpcell.00393.2019⟩
Article dans une revue hal-02465757v1
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PET-MRI nanoparticles imaging of blood–brain barrier damage and modulation after stroke reperfusion

Justine Debatisse , Omer Faruk Eker , Océane Wateau , Tae-Hee Cho , Marlene Wiart
Brain Communications, 2020, 2 (2), ⟨10.1093/braincomms/fcaa193⟩
Article dans une revue hal-03060618v1
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Reduced reticulum–mitochondria Ca2+ transfer is an early and reversible trigger of mitochondrial dysfunctions in diabetic cardiomyopathy

Maya Dia , Ludovic Gomez , Helene Thibault , Nolwenn Tessier , Christelle Leon
Basic Research in Cardiology, 2020, 115 (6), pp.74. ⟨10.1007/s00395-020-00835-7⟩
Article dans une revue hal-03122630v1

A novel class of cardioprotective small-molecule PTP inhibitors

S. Antonucci , M. Di Sante , J. Sileikyte , J. Deveraux , T. Bauer
Pharmacological Research, 2020, 151, pp.104548. ⟨10.1016/j.phrs.2019.104548⟩
Article dans une revue hal-02901734v1
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Critical appraisal of STAT3 pattern in adult cardiomyocytes

Zeina Harhous , Sally Badawi , Noelle Gallo Bona , Bruno Pillot , Lionel Augeul
Journal of Molecular and Cellular Cardiology, 2019, 131, pp.91-100. ⟨10.1016/j.yjmcc.2019.04.021⟩
Article dans une revue hal-03060376v1

1-Deoxydihydroceramide causes anoxic death by impairing chaperonin-mediated protein folding

J. T. Hannich , A. G. Haribowo , S. Gentina , M. Paillard , L. Gomez
Nature Metabolism, 2019, 1 (10), pp.996--+. ⟨10.1038/s42255-019-0123-y⟩
Article dans une revue hal-02465918v1

Critical appraisal of STAT3 pattern in adult cardiomyocytes

Z. Harhous , S. Badawi , N. G. Bona , B. Pillot , L. Augeul
Journal of Molecular and Cellular Cardiology, 2019, 131, pp.91--100. ⟨10.1016/j.yjmcc.2019.04.021⟩
Article dans une revue hal-02195242v1

MICU1 Interacts with the D-Ring of the MCU Pore to Control Its Ca2+ Flux and Sensitivity to Ru360

Melanie Paillard , György Csordás , Kai-Ting Huang , Peter Várnai , Suresh Joseph
Molecular Cell, 2018, 72 (4), pp.778-785.e3. ⟨10.1016/j.molcel.2018.09.008⟩
Article dans une revue hal-03313043v1

4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+ transfer

Gabriel Bidaux , Dmitri Gordienko , George Shapovalov , Valerio Farfariello , Anne-Sophie Borowiec
Biochimica et Biophysica Acta - Molecular Cell Research, 2018, 1865 (7), pp.981-994. ⟨10.1016/j.bbamcr.2018.04.007⟩
Article dans une revue hal-01847399v1

Mitochondrial fusion dynamics is robust in the heart and depends on calcium oscillations and contractile activity

V. Eisner , R. R. Cupo , E. Gao , G. Csordas , W. S. Slovinsky
Proceedings of the National Academy of Sciences of the United States of America, 2017, 114 (5), pp.E859-E868. ⟨10.1073/pnas.1617288114⟩
Article dans une revue hal-01848305v1

Tissue-Specific Mitochondrial Decoding of Cytoplasmic Ca 2+ Signals Is Controlled by the Stoichiometry of MICU1/2 and MCU

Melanie Paillard , György Csordás , Gergö Szanda , Tünde Golenár , Valentina Debattisti
Cell Reports, 2017, 18 (10), pp.2291-2300. ⟨10.1016/j.celrep.2017.02.032⟩
Article dans une revue hal-03313040v1

MICU1 regulation of mitochondrial Ca2+ uptake dictates survival and tissue regeneration

Anil Noronha Antony , Melanie Paillard , Cynthia Moffat , Egle Juskeviciute , Jason Correnti
Nature Communications, 2016, 7 (1), ⟨10.1038/ncomms10955⟩
Article dans une revue hal-03313035v1
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Disruption of calcium transfer from ER to mitochondria links alterations of mitochondria-associated ER membrane integrity to hepatic insulin resistance

Jennifer Rieusset , J. Fauconnier , Mélanie Paillard , Elise Belaidi , Emily Tubbs
Diabetologia, 2016, 59 (3), pp.614-623. ⟨10.1007/s00125-015-3829-8⟩
Article dans une revue hal-01786231v1

The SR/ER-mitochondria calcium crosstalk is regulated by GSK3beta during reperfusion injury

L. Gomez , P. A. Thiebaut , M. Paillard , S. Ducreux , M. Abrial
Cell Death and Differentiation, 2016, 23 (2), pp.313-22. ⟨10.1038/cdd.2015.101⟩
Article dans une revue hal-01850428v1

Reliance of ER–mitochondrial calcium signaling on mitochondrial EF-hand Ca2+ binding proteins: Miros, MICUs, LETM1 and solute carriers

György Hajnóczky , David Booth , György Csordás , Valentina Debattisti , Tünde Golenár
Current Opinion in Cell Biology, 2014, 29, pp.133-141. ⟨10.1016/j.ceb.2014.06.002⟩
Article dans une revue hal-03312949v1

Depressing Mitochondria-Reticulum Interactions Protects Cardiomyocytes From Lethal Hypoxia-Reoxygenation Injury

Melanie Paillard , Emily Tubbs , Pierre-Alain Thiebaut , Ludovic Gomez , J. Fauconnier
Circulation, 2013, 128 (14), pp.1555-1565. ⟨10.1161/CIRCULATIONAHA.113.001225⟩
Article dans une revue hal-03312946v1

Postconditioning Modulates Ischemia-damaged Mitochondria During Reperfusion

Qun Chen , Melanie Paillard , Ludovic Gomez , Heng Li , Ying Hu
Journal of Cardiovascular Pharmacology, 2012, 59 (1), pp.101-108. ⟨10.1097/FJC.0b013e31823827cc⟩
Article dans une revue hal-03312942v1

Inhibition of mitochondrial permeability transition to prevent the post-cardiac arrest syndrome: a pre-clinical study

M. Cour , J. Loufouat , M. Paillard , L. Augeul , J. Goudable
European Heart Journal, 2011, 32 (2), pp.226-235. ⟨10.1093/eurheartj/ehq112⟩
Article dans une revue hal-03312939v1

A novel role for mitochondrial sphingosine-1-phosphate produced by sphingosine kinase-2 in PTP-mediated cell survival during cardioprotection

Ludovic Gomez , Melanie Paillard , Megan Price , Qun Chen , Geoffrey Teixeira
Basic Research in Cardiology, 2011, 106 (6), pp.1341-1353. ⟨10.1007/s00395-011-0223-7⟩
Article dans une revue hal-03312943v1

Activation of mitochondrial μ-calpain increases AIF cleavage in cardiac mitochondria during ischemia–reperfusion

Qun Chen , Melanie Paillard , Ludovic Gomez , Thomas Ross , Ying Hu
Biochemical and Biophysical Research Communications, 2011, 415 (4), pp.533-538. ⟨10.1016/j.bbrc.2011.10.037⟩
Article dans une revue hal-03312945v1

Sphingosine‐1‐phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration

Graham Strub , Melanie Paillard , Jie Liang , Ludovic Gomez , Jeremy Allegood
FASEB Journal, 2010, 25 (2), pp.600-612. ⟨10.1096/fj.10-167502⟩
Article dans une revue hal-03312941v1

Postconditioning inhibits mPTP opening independent of oxidative phosphorylation and membrane potential

Melanie Paillard , Ludovic Gomez , Lionel Augeul , Joseph Loufouat , Edward Lesnefsky
Journal of Molecular and Cellular Cardiology, 2009, 46 (6), pp.902-909. ⟨10.1016/j.yjmcc.2009.02.017⟩
Article dans une revue hal-03312937v1