Vladimir SHEVCHIK

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Publications

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An inner membrane protein is covalently attached to peptidoglycan in the γ-proteobacterium Dickeya dadantii

Xavier Nicolai , Yucheng Liang , Florence Ruaudel , Magdalena Narajczyk , Robert Czajkowski et al.

Communications Biology, 2025, 8 (1), pp.1071. ⟨10.1038/s42003-025-08488-9⟩

Article dans une revue hal-05191598v1
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Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall

Shiheng Zhang , Shuang Gu , Piers Rycroft , Florence Ruaudel , Frédéric Delolme et al.

mBio, 2022, 13 (3), pp.e0025322. ⟨10.1128/mbio.00253-22⟩

Article dans une revue hal-03748109v2
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Secondary structure and 1H, 15 N & 13C resonance assignments of the periplasmic domain of OutG, major pseudopilin from Dickeya dadantii type II secretion system

Theis Jacobsen , Régine Dazzoni , Melvin Renault , Benjamin Bardiaux , Michael Nilges et al.

Biomolecular NMR Assignments, 2022, 16 (2), pp.231-236. ⟨10.1007/s12104-022-10085-4⟩

Article dans une revue hal-03836613v1
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Structure–function analysis of pectate lyase Pel3 reveals essential facets of protein recognition by the bacterial type 2 secretion system.

Camille Pineau , Natalia Guschinskaya , Isabelle R Gonçalves , Florence Ruaudel , Xavier Robert et al.

Journal of Biological Chemistry, 2021, pp.100305. ⟨10.1016/j.jbc.2021.100305⟩

Article dans une revue hal-03143879v1

Dickeya dadantii pectic enzymes necessary for virulence are also responsible for activation of the Arabidopsis thaliana innate immune system

D. Expert , O. Patrit , Vladimir Shevchik , C. Perino , V. Boucher et al.

Molecular Plant Pathology, 2018, 19 (2), pp.313-327. ⟨10.1111/mpp.12522⟩

Article dans une revue hal-02001363v1

The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system

S. Gu , Vladimir Shevchik , R. Shaw , R. W. Pickersgill , J. A. Garnett

Biochim Biophys Acta Proteins Proteom, 2017, 1865 (10), pp.1255-1266. ⟨10.1016/j.bbapap.2017.07.006⟩

Article dans une revue hal-02001349v1

Bacterial pectate lyases, structural and functional diversity

Nicole Hugouvieux-Cotte-Pattat , Guy Condemine , Vladimir Shevchik

Environ Microbiol Rep, 2014, 6 (5), pp.427-40

Article dans une revue hal-02001305v1

Substrate recognition by the bacterial type II secretion system: more than a simple interaction

Christophe Pineau , Natalia Guschinskaya , X. Robert , P. Gouet , L. Ballut et al.

Molecular Microbiology, 2014, 94 (1), pp.126-40. ⟨10.1111/mmi.12744⟩

Article dans une revue hal-02001308v1

PelN is a new pectate lyase of Dickeya dadantii with unusual characteristics

S. Hassan , Vladimir Shevchik , X. Robert , Nicole Hugouvieux-Cotte-Pattat

Journal of Bacteriology, 2013, 195 (10), pp.2197-206. ⟨10.1128/JB.02118-12⟩

Article dans une revue hal-02001296v1

Anatomy of secretin binding to the Dickeya dadantii type II secretion system pilotin

S. Rehman , S. Gu , Vladimir Shevchik , R. W. Pickersgill

Acta Crystallogr D Biol Crystallogr, 2013, 69 (Pt 8), pp.1381-6

Article dans une revue hal-02001300v1
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Dynamic interplay between the periplasmic and transmembrane domains of GspL and GspM in the type II secretion system

Mathilde Lallemand , Frédéric H. Login , Natalia Guschinskaya , Camille Pineau , Géraldine Effantin et al.

PLoS ONE, 2013, 8 (11), pp.e79562. ⟨10.1371/journal.pone.0079562⟩

Article dans une revue hal-02001297v1
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Cysteine scanning mutagenesis and disulfide mapping analysis of arrangement of GspC and GspD protomers within the type 2 secretion system

X. Wang , C. Pineau , S. Gu , Natalia Guschinskaya , R. W. Pickersgill et al.

Journal of Biological Chemistry, 2012, 287 (23), pp.19082-93. ⟨10.1074/jbc.M112.346338⟩

Article dans une revue hal-02001294v1

Solution structure of homology region (HR) domain of type II secretion system

S. Gu , G. Kelly , X. Wang , T. Frenkiel , Vladimir Shevchik et al.

Journal of Biological Chemistry, 2012, 287 (12), pp.9072-80

Article dans une revue hal-02001291v1

Structural and functional insights into the pilotin-secretin complex of the type II secretion system

S. Gu , S. Rehman , X. Wang , Vladimir Shevchik , R. W. Pickersgill

PLoS Pathogens, 2012, 8 (2), pp.e1002531. ⟨10.1371/journal.ppat.1002531⟩

Article dans une revue hal-02001292v1
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A 20 residue peptide of the inner membrane protein OutC mediates interaction with two distinct sites of the outer membrane secretin OutD and is essential for the functional type II secretion system in Erwinia chrysanthemi.

Frédéric H. Login , Markus Fries , Xiaohui Wang , Richard W. Pickersgill , Vladimir Shevchik

Molecular Microbiology, 2010, 76 (4), pp.944. ⟨10.1111/j.1365-2958.2010.07149.x⟩

Article dans une revue hal-00552630v1
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The crystal structure of pectate lyase peli from soft rot pathogen Erwinia chrysanthemi in complex with its substrate

Christophe Crezé , Sandra Castang , Emmanuel Derivery , Richard Haser , Nicole Hugouvieux-Cotte-Pattat et al.

Journal of Biological Chemistry, 2008, 283 (26), pp.18260-18268. ⟨10.1074/jbc.M709931200⟩

Article dans une revue hal-00315227v1
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Arabidopsis thaliana expresses multiple lines of defense to counterattack Erwinia chrysanthemi

Mathilde M. Fagard , Alia Dellagi , Camille Roux , Claude Perino , Martine Rigault et al.

Molecular Plant-Microbe Interactions, 2007, 20 (7), pp.794-805. ⟨10.1094/MPMI-20-7-0794⟩

Article dans une revue hal-02659246v1

Molecular basis of the activity of the phytopathogen pectin methylesterase

Markus Fries , Jessica Ihrig , Keith Brocklehurst , Vladimir Shevchik , Richard Pickersgill

EMBO Journal, 2007, 26 (17), pp.3879-3887. ⟨10.1038/sj.emboj.7601816⟩

Article dans une revue hal-03102433v1

The Single Transmembrane Segment Drives Self-assembly of OutC and the Formation of a Functional Type II Secretion System in Erwinia chrysanthemi

Frédéric Login , Vladimir Shevchik

Journal of Biological Chemistry, 2006, 281 (44), pp.33152-33162. ⟨10.1074/jbc.M606245200⟩

Article dans une revue hal-03102756v1

The Crystal Structure of Pectate Lyase Pel9A from Erwinia chrysanthemi

John Jenkins , Vladimir Shevchik , Nicole Hugouvieux-Cotte-Pattat , Richard Pickersgill

Journal of Biological Chemistry, 2004, 279 (10), pp.9139-9145. ⟨10.1074/jbc.M311390200⟩

Article dans une revue hal-03102761v1

Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative.

S. Castang , Ve Shevchik , N. Hugouvieux-Cotte-Pattat , P. Legrand , R. Haser et al.

Acta Crystallogr D Biol Crystallogr, 2004, 60, pp.190-192

Article dans une revue hal-00313538v1

PaeX, a Second Pectin Acetylesterase of Erwinia chrysanthemi 3937

Vladimir Shevchik , Nicole Hugouvieux-Cotte-Pattat

Journal of Bacteriology, 2003, 185 (10), pp.3091-3100. ⟨10.1128/jb.185.10.3091-3100.2003⟩

Article dans une revue hal-03102763v1

Characterization of pectin methylesterase B, an outer membrane lipoprotein of Erwinia chrysanthemi 3937

Vladimir Shevchik , Guy Condemine , Nicole Hugouvieux-Cotte-Pattat , Janine Robert‐baudouy

Molecular Microbiology, 2003, 19 (3), pp.455-466. ⟨10.1046/j.1365-2958.1996.389922.x⟩

Article dans une revue hal-03102867v1

Identification of a bacterial pectin acetyl esterase in Erwinia chrysanthemi 3937

Vladimir Shevchik , Nicole Hugouvieux-Cotte-Pattat

Molecular Microbiology, 2003, 24 (6), pp.1285-1301. ⟨10.1046/j.1365-2958.1997.4331800.x⟩

Article dans une revue hal-03102863v1

Processing of the pectate lyase PelI by extracellular proteases of Erwinia chrysanthemi 3937

Vladimir Shevchik , Martine Boccara , Régine Vedel , Nicole Hugouvieux-Cotte-Pattat

Molecular Microbiology, 2002, 29 (6), pp.1459-1469. ⟨10.1046/j.1365-2958.1998.01028.x⟩

Article dans une revue hal-03102839v1

PehN, a Polygalacturonase Homologue with a Low Hydrolase Activity, Is Coregulated with the Other Erwinia chrysanthemi Polygalacturonases

Nicole Hugouvieux-Cotte-Pattat , Vladimir Shevchik , William Nasser

Journal of Bacteriology, 2002, 184 (10), pp.2664-2673. ⟨10.1128/jb.184.10.2664-2673.2002⟩

Article dans une revue hal-03102765v1

The PDZ domain of OutC and the N-terminal region of OutD determine the secretion specificity of the type II out pathway of Erwinia chrysanthemi1 1Edited by I. B. Holland

Julien Bouley , Guy Condemine , Vladimir Shevchik

Journal of Molecular Biology, 2001, 308 (2), pp.205-219. ⟨10.1006/jmbi.2001.4594⟩

Article dans une revue istex hal-03102769v1

Overproduction of the secretin OutD suppresses the secretion defect of an Erwinia chrysanthemi outB mutant

Guy Condemine , Vladimir Shevchik

Microbiology, 2000, 146 (3), pp.639-647. ⟨10.1099/00221287-146-3-639⟩

Article dans une revue hal-03102781v1

Characterization of the Exopolygalacturonate Lyase PelX of Erwinia chrysanthemi 3937

Vladimir Shevchik , Harry Kester , Jacques Benen , Jaap Visser , Janine Robert-Baudouy et al.

Journal of Bacteriology, 1999, 181 (5), pp.1652-1663. ⟨10.1128/JB.181.5.1652-1663.1999⟩

Article dans une revue hal-03102812v1

Modes of Action of Five Different Endopectate Lyases from Erwinia chrysanthemi 3937

Caroline Roy , Harry Kester , Jaap Visser , Vladimir Shevchik , Nicole Hugouvieux-Cotte-Pattat et al.

Journal of Bacteriology, 1999, 181 (12), pp.3705-3709. ⟨10.1128/JB.181.12.3705-3709.1999⟩

Article dans une revue hal-03102810v1

Analysis of three clustered polygalacturonase genes in Erwinia chrysanthemi 3937 revealed an anti-repressor function for the PecS regulator

William Nasser , Vladimir Shevchik , Nicole Hugouvieux-Cotte-Pattat

Molecular Microbiology, 1999, 34 (4), pp.641-650. ⟨10.1046/j.1365-2958.1999.01609.x⟩

Article dans une revue hal-03102793v1

The Exopolygalacturonate Lyase PelW and the Oligogalacturonate Lyase Ogl, Two Cytoplasmic Enzymes of Pectin Catabolism in Erwinia chrysanthemi 3937

Vladimir Shevchik , Guy Condemine , Janine Robert-Baudouy , Nicole Hugouvieux-Cotte-Pattat

Journal of Bacteriology, 1999, 181 (13), pp.3912-3919. ⟨10.1128/JB.181.13.3912-3919.1999⟩

Article dans une revue hal-03102804v1

Performance of Selected Microbial Pectinases on Synthetic Monomethyl-esterified Di- and Trigalacturonates

Harry Kester , Didier Magaud , Caroline Roy , Daniel Anker , Alain Doutheau et al.

Journal of Biological Chemistry, 1999, 274 (52), pp.37053-37059. ⟨10.1074/jbc.274.52.37053⟩

Article dans une revue hal-03102789v1

Functional characterization of the Erwinia chrysanthemi Outs protein, an element of a type II secretion system

Vladimir Shevchik , Guy Condemine

Microbiology, 1998, 144 (11), pp.3219-3228. ⟨10.1099/00221287-144-11-3219⟩

Article dans une revue hal-03102821v1

Crystallization and preliminary X-ray analysis of a member of a new family of pectate lyases, PeIL from Erwinia chrysanthemi

Vladimir Shevchik , Mandy Scott , Olga Mayans , John Jenkins

Acta crystallographica Section D : Structural biology [1993-..], 1998, 54 (3), pp.419-422. ⟨10.1107/s0907444997012043⟩

Article dans une revue istex hal-03102845v1

Synthesis of the two monomethyl esters of the disaccharide 4-O-α-d-galacturonosyl-d-galacturonic acid and of precursors for the preparation of higher oligomers methyl uronated in definite sequences

Didier Magaud , Cyrille Grandjean , Alain Doutheau , Daniel Anker , Vladimir Shevchik et al.

Carbohydrate Research, 1998, 314 (3-4), pp.189-199. ⟨10.1016/S0008-6215(98)00312-7⟩

Article dans une revue istex hal-02197034v1

An efficient and highly stereoselective α(1→4) glycosylation between two d-galacturonic acid ester derivatives

Didier Magaud , Cyrille Grandjean , Alain Doutheau , Daniel Anker , Vladimir Shevchik et al.

Tetrahedron Letters, 1997, 38 (2), pp.241-244. ⟨10.1016/S0040-4039(96)02308-8⟩

Article dans une revue istex hal-02197035v1

Specific interaction between OutD, an Erwinia chrysanthemi outer membrane protein of the general secretory pathway, and secreted proteins

Vladimir Shevchik , J Robert-Baudouy , Guy Condemine

EMBO Journal, 1997, 16 (11), pp.3007-3016. ⟨10.1093/emboj/16.11.3007⟩

Article dans une revue hal-03102856v1

Pectate lyase PelI of Erwinia chrysanthemi 3937 belongs to a new family.

Vladimir Shevchik , Janine Robert-Baudouy , Nicole Hugouvieux-Cotte-Pattat

Journal of Bacteriology, 1997, 179 (23), pp.7321-7330. ⟨10.1128/jb.179.23.7321-7330.1997⟩

Article dans une revue hal-03102849v1

Differential effect of dsbA and dsbC mutations on extracellular enzyme secretion in Erwinia chrysanthemi

Vladimir Shevchik , Isabelle Bortoli-Gernnan , Janine Robert-Baudouy , Sandrine Robinet , Frédéric Barras et al.

Molecular Microbiology, 1995, 16 (4), pp.745-753. ⟨10.1111/j.1365-2958.1995.tb02435.x⟩

Article dans une revue hal-03102871v1

Characterization of DsbC, a periplasmic protein of Erwinia chrysanthemi and Escherichia coli with disulfide isomerase activity.

Vladimir Shevchik , Guy Condemine , Janine Robert-Baudouy

EMBO Journal, 1994, 13 (8), pp.2007-12

Article dans une revue hal-03102873v1

Production of pectolytic enzymes from Erwinia grown on different carbon sources

Vladimir Shevchik , A. Evtushenkov , H. Babitskaya , Y. Fomichev

World Journal of Microbiology and Biotechnology, 1992, 8 (2), pp.115-120. ⟨10.1007/BF01195828⟩

Article dans une revue istex hal-03102875v1