Xavier ROBERT

Ph.D., Ingénieur de Recherche (IR) au CNRS
67%
Libre accès
54
Documents
Affiliation actuelle
  • Microbiologie moléculaire et biochimie structurale - Molecular Microbiology and Structural Biochemistry (MMSB)
Identifiants chercheurs
Contact

Présentation

Xavier Robert est Ingénieur de Recherche (IR) au sein de l'Unité "Microbiologie Moléculaire et Biochimie Structurale" (MMSB, UMR5086, CNRS / Université de Lyon, France) depuis 2001. Il a obtenu sa Maîtrise puis son Doctorat en Biochimie à l'Université Claude Bernard Lyon 1 en 1998 et 2002. Xavier Robert effectue des recherches en rétrovirologie en utilisant des méthodes de biologie structurale (cristallographie aux rayons X) et de bio-informatique (simulations assistées par ordinateur, docking moléculaire et modélisation) ainsi que le génie logiciel. Il est co-développeur des services web ESPript, ENDscript et FoldScript (https://espript.ibcp.fr, https://endscript.ibcp.fr & https://foldscript.ibcp.fr).

Domaines de recherche

Biologie structurale [q-bio.BM] Bio-informatique [q-bio.QM] Modélisation et simulation Génie logiciel [cs.SE]

Compétences

Protein X-ray Crystallography Structural Biology Structural Bioinformatics Bioinformatics Protein Structure Molecular Simulation Molecular Docking Molecular Modeling Software Engineering Protein Sequence Analysis

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Publications

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Biochemical and Biophysical Research Communications Integrating different approaches for the identification of new disruptors of HIV-1 capsid multimerization

Zoraima Artía , Christophe Guillon , Xavier Robert , Maximiliano Granzella , Ana Clara Segovia et al.
Biochemical and Biophysical Research Communications, 2025, 763, pp.151572. ⟨10.1016/j.bbrc.2025.151572⟩
Article dans une revue hal-05035946 v1
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FoldScript: a web server for the efficient analysis of AI-generated 3D protein models

Xavier Robert , Christophe Guillon , Patrice Gouet
Nucleic Acids Research, 2025, 23rd annual Nucleic Acids Research Web Server Issue 2025, ⟨10.1093/nar/gkaf326⟩
Article dans une revue hal-05049646 v1
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“It’s Only a Model”: When Protein Structure Predictions Need Experimental Validation, the Case of the HTLV-1 Tax Protein

Christophe Guillon , Xavier Robert , Patrice Gouet
Pathogens, 2024, 13 (3), pp.241. ⟨10.3390/pathogens13030241⟩
Article dans une revue (article de synthèse) hal-04497855 v1
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Unveiling a Shield of Hope: A Novel Multiepitope-Based Immunogen for Cross-Serotype Cellular Defense against Dengue Virus

Nilanshu Manocha , Daphné Laubreton , Xavier Robert , Jacqueline Marvel , Virginie Gueguen-Chaignon et al.
Vaccines, 2024, 12 (3), pp.316. ⟨10.3390/vaccines12030316⟩
Article dans une revue hal-04902274 v1
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Purine containing carbonucleoside phosphonate analogues as novel chemotype for Plasmodium falciparum Inhibition

Bemba Sidi Mohamed , Minh Chau Nguyen , Sharon Wein , Jean-Pierre Uttaro , Xavier Robert et al.
European Journal of Medicinal Chemistry, 2023, 258, pp.115581. ⟨10.1016/j.ejmech.2023.115581⟩
Article dans une revue hal-04269850 v1
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Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall

Shiheng Zhang , Shuang Gu , Piers Rycroft , Florence Ruaudel , Frédéric Delolme et al.
mBio, 2022, 13 (3), pp.e0025322. ⟨10.1128/mbio.00253-22⟩
Article dans une revue hal-03748109 v2
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Identification of 2 -(4 -N ,N -Di methyl aminop henyl) -5-methyl -1-phenethyl -1H-benzimidazole targeting HIV-1 CA capsid protein and inhibiting HIV-1 replication in cellulo

Guzmán Alvarez , Lisa van Pul , Xavier Robert , Zoraima Artía , Ad C van Nuenen et al.
BMC Pharmacology and Toxicology, 2022, 23, pp.43. ⟨10.1186/s40360-022-00581-7⟩
Article dans une revue hal-03708244 v1
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Specific Xray diffraction patterns of membrane proteins caused by secondary structure collinearity

Juliette Martin , Xavier Robert , Patrice Gouet , Pierre Falson , Vincent Chaptal
Biochimica et Biophysica Acta:Biomembranes, 2022, pp.184065. ⟨10.1016/j.bbamem.2022.184065⟩
Article dans une revue hal-03796617 v1
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Identification of a Potential Inhibitor of the FIV p24 Capsid Protein and Characterization of Its Binding Site

Mathieu Long , François-Xavier Cantrelle , Xavier Robert , Emmanuelle Boll , Natalia Sierra et al.
Biochemistry, 2021, 60 (24), pp.1896-1908. ⟨10.1021/acs.biochem.1c00228⟩
Article dans une revue hal-03280829 v1
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CryoEM reconstructions of membrane proteins solved in several amphipathic solvents, nanodisc, amphipol and detergents, yield amphipathic belts of similar sizes corresponding to a common ordered solvent layer

Veronica Zampieri , Alexia Gobet , Xavier Robert , Pierre Falson , Vincent Chaptal
Biochimica et Biophysica Acta:Biomembranes, 2021, 1863 (11), pp.183693. ⟨10.1016/j.bbamem.2021.183693⟩
Article dans une revue hal-03453888 v1
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Structure–function analysis of pectate lyase Pel3 reveals essential facets of protein recognition by the bacterial type 2 secretion system.

Camille Pineau , Natalia Guschinskaya , Isabelle R Gonçalves , Florence Ruaudel , Xavier Robert et al.
Journal of Biological Chemistry, 2021, pp.100305. ⟨10.1016/j.jbc.2021.100305⟩
Article dans une revue hal-03143879 v1
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Pyrazol(in)e derivatives of curcumin analogs as a new class of anti- Trypanosoma cruzi agents

Dimitris Matiadis , Tatiana Saporiti , Elena Aguilera , Xavier Robert , Christophe Guillon et al.
Future Medicinal Chemistry, 2021, 13 (8), pp.701-714. ⟨10.4155/fmc-2020-0349⟩
Article dans une revue hal-03230237 v1
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Exploring molecular determinants of polysaccharide lyase family 6–1 enzyme activity

Sébastien Violot , Frédéric Galisson , Loïc Carrique , Vinesh Jugnarain , Léa Conchou et al.
Glycobiology, 2021, ⟨10.1093/glycob/cwab073⟩
Article dans une revue hal-03379698 v1
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Novel and selective inactivators of Triosephosphate isomerase with anti-trematode activity

Florencia Ferraro , Ileana Corvo , Lucia Bergalli , Andrea Ilarraz , Mauricio Cabrera et al.
Scientific Reports, 2020, 10 (1), ⟨10.1038/s41598-020-59460-y⟩
Article dans une revue hal-02571943 v1
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Human H4 tail stimulates HIV-1 integration through binding to the carboxy-terminal domain of integrase

Eric Mauro , Paul Lesbats , Delphine Lapaillerie , Stéphane Chaignepain , Benoit Maillot et al.
Nucleic Acids Research, 2019, 47 (7), pp.3607-3618. ⟨10.1093/nar/gkz091⟩
Article dans une revue hal-02122315 v1
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Au courant computation of the PDB to audit diffraction anisotropy of soluble and membrane proteins

Xavier Robert , Josiane Kassis-Sahyoun , Nicoletta Ceres , Juliette Martin , Michael R Sawaya et al.
Data in Brief, 2018, 19, pp.753-757. ⟨10.1016/j.dib.2018.05.072⟩
Article dans une revue hal-02326014 v1
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Looking for Novel Capsid Protein Multimerization Inhibitors of Feline Immunodeficiency Virus

Natalia Sierra , Christelle Folio , Xavier Robert , Mathieu Long , Christophe Guillon et al.
Pharmaceuticals, 2018, 11 (3), pp.67. ⟨10.3390/ph11030067⟩
Article dans une revue hal-02121651 v1
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Identification and visualization of protein binding regions with the ArDock server

Sébastien Reille , Mélanie Garnier , Xavier Robert , Patrice Gouet , Juliette Martin et al.
Nucleic Acids Research, 2018, 46 (W1), pp.W417-W422. ⟨10.1093/nar/gky472⟩
Article dans une revue hal-02344721 v1
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X-ray diffraction reveals the intrinsic difference in the physical properties of membrane and soluble proteins

Xavier Robert , Josiane Kassis-Sahyoun , Nicoletta Ceres , Juliette Martin , Michael Sawaya et al.
Scientific Reports, 2017, 7 (1), ⟨10.1038/s41598-017-17216-1⟩
Article dans une revue hal-02110047 v1
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Modulation of the functional association between the HIV-1 intasome and the nucleosome by histone amino-terminal tails

Mohamed S Benleulmi , Julien Matysiak , Xavier Robert , Csaba Miskey , Eric Mauro et al.
Retrovirology, 2017, 14 (1), pp.54. ⟨10.1186/s12977-017-0378-x⟩
Article dans une revue hal-03532360 v1

Substrate recognition by the bacterial type II secretion system: more than a simple interaction

C. Pineau , Natalia Guschinskaya , X. Robert , P. Gouet , L. Ballut et al.
Molecular Microbiology, 2014, 94 (1), pp.126-40. ⟨10.1111/mmi.12744⟩
Article dans une revue hal-02001308 v1
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Deciphering key features in protein structures with the new ENDscript server

Xavier Robert , Patrice Gouet
Nucleic Acids Research, 2014, Issue W1, 42 (W1), pp.W320-W324. ⟨10.1093/nar/gku316⟩
Article dans une revue hal-04909755 v1

PelN is a new pectate lyase of Dickeya dadantii with unusual characteristics

S. Hassan , Vladimir Shevchik , X. Robert , Nicole Hugouvieux-Cotte-Pattat
Journal of Bacteriology, 2013, 195 (10), pp.2197-206. ⟨10.1128/JB.02118-12⟩
Article dans une revue hal-02001296 v1

Biophysical characterization and crystal structure of the Feline Immunodeficiency Virus p15 matrix protein

Jennifer Serrière , Xavier Robert , Magali Perez , Patrice Gouet , Christophe Guillon
Retrovirology, 2013, 10 (1), pp.64. ⟨10.1186/1742-4690-10-64⟩
Article dans une revue hal-02121708 v1

Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity

Alexandra Lipski , Hildegard Watzlawick , Stéphanie Ravaud , Xavier Robert , Moez Rhimi et al.
Acta crystallographica Section D : Structural biology [1993-..], 2013, 69 (Pt 2), pp.298-307. ⟨10.1107/S0907444912045532⟩
Article dans une revue istex hal-01204490 v1
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Dynamic interplay between the periplasmic and transmembrane domains of GspL and GspM in the type II secretion system

Mathilde Lallemand , Frédéric H. Login , Natalia Guschinskaya , Camille Pineau , Géraldine Effantin et al.
PLoS ONE, 2013, 8 (11), pp.e79562. ⟨10.1371/journal.pone.0079562⟩
Article dans une revue hal-02001297 v1
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A crystal structure of the catalytic core domain of an avian sarcoma and leukemia virus integrase suggests an alternate dimeric assembly

Allison Ballandras , Karen Moreau , Xavier Robert , Marie-Pierre Confort , Romain Merceron et al.
PLoS ONE, 2011, 6 (8), pp.1 - 10. ⟨10.1371/journal.pone.0023032⟩
Article dans une revue hal-02645754 v1

The Extended Conformation of the 2.9-Å Crystal Structure of the Three-PASTA Domain of a Ser/Thr Kinase from the Human Pathogen Staphylococcus aureus

Patricia Paracuellos , Allison Ballandras , Xavier Robert , Richard Kahn , Mireille Hervé et al.
Journal of Molecular Biology, 2010, 404 (5), pp.847-858. ⟨10.1016/j.jmb.2010.10.012⟩
Article dans une revue istex hal-04246133 v1

Structural determinants of product specificity of sucrose isomerases

Stéphanie Ravaud , Xavier Robert , Hildegard Watzlawick , Richard Haser , Ralf Mattes et al.
FEBS Letters, 2009, 583 (12), pp.1964-1968. ⟨10.1016/j.febslet.2009.05.002⟩
Article dans une revue istex hal-03094606 v1

Crystallization and initial X-ray diffraction study of the three PASTA domains of the Ser/Thr kinase Stk1 from the human pathogen Staphylococcus aureus

Patricia Paracuellos , Allison Ballandras , Xavier Robert , Alain Cozzone , Bertrand Duclos et al.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2009, 65 (11), pp.1187-1189. ⟨10.1107/s174430910904250x⟩
Article dans une revue hal-04246128 v1

C-terminal Residues Regulate Localization and Function of the Antiapoptotic Protein Bfl-1

Gaelle Brien , Anne-Laure Debaud , Xavier Robert , Lisa Oliver , Marie-Claude Trescol-Biemont et al.
Journal of Biological Chemistry, 2009, 284 (44), pp.30257-30263. ⟨10.1074/jbc.M109.040824⟩
Article dans une revue hal-04911284 v1

Multi-site substrate binding and interplay in barley alpha-amylase 1.

Mm Nielsen , Es Seo , Sophie Bozonnet , N. Aghajari , X. Robert et al.
FEBS Letters, 2008, 582, pp.2567-2571
Article dans une revue hal-00315255 v1

Insights into sucrose isomerization from crystal structures of the Pseudomonas mesoacidophila MX-45 sucrose isomerase, MutB

S. Ravaud , X. Robert , H. Watzlawick , S. Laurent , R. Haser et al.
Biocatalysis and Biotransformation, 2008, 26, pp.111-119
Article dans une revue hal-00315188 v1

Trehalulose Synthase Native and Carbohydrate Complexed Structures Provide Insights into Sucrose Isomerization

Stephanie Ravaud , Xavier Robert , Hildegard Watzlawick , Richard Haser , Ralf Mattes et al.
Journal of Biological Chemistry, 2007, 282 (38), pp.28126-28136. ⟨10.1074/jbc.M704515200⟩
Article dans une revue hal-04914520 v1

Sugar-converting enzymes: new insights into structures and mechanisms

Nushin Aghajari , Stephanie Ravaud , Xavier Robert , Hildegard Watzlawick , Birte Svensson et al.
Acta Crystallographica Section A : Foundations of Crystallography [1968-2013], 2007, 63 (a1), pp.s136-s136. ⟨10.1107/S0108767307097000⟩
Article dans une revue hal-04914571 v1

Interactions between Barley α-Amylases, Substrates, Inhibitors and Regulatory Proteins

Maher Abou Hachem , Sophie Bozonnet , Martin Willemoës , Birgit C. Bønsager , Morten Munch Nielsen et al.
Journal of applied Glycoscience, 2006, 53 (2), pp.163-169. ⟨10.5458/jag.53.163⟩
Article dans une revue hal-04914660 v1

Interactions of barley alpha-amylase isozymes with Ca2+, substrates and proteinaceous inhibitors

M. Abouhachem , Sophie Bozonnet , M. Willemoes , B. Kramhoft , K. Fukuda et al.
Biocatalysis and Biotransformation, 2006, 24, pp.83-93
Article dans une revue hal-00314839 v1

Thermostability enhancement and change in starch hydrolysis profile of the maltohexaose-forming amylase of Bacillus stearothermophilus US100 strain.

M. Benali , B. Khemakhem , X. Robert , R. Haser , S. Bejar
Biochemical Journal, 2006, 394, pp.51-56
Article dans une revue hal-00313531 v1

ESPript/ENDscript: sequence and 3D information from protein structures

Patrice Gouet , Xavier Robert , Emmanuel Courcelle
Acta Crystallographica Section A : Foundations of Crystallography [1968-2013], 2005, 61 (a1), pp.c42-c43. ⟨10.1107/S0108767305098211⟩
Article dans une revue hal-04909788 v1

Oligosaccharide binding to barley alpha-amylase 1.

X. Robert , R. Haser , H. Mori , B. Svensson , N. Aghajari
Journal of Biological Chemistry, 2005, 280, pp.32968-32978
Article dans une revue hal-00313533 v1

Binding of carbohydrates and protein inhibitors to the surface of alpha-amylases

Sophie Bozonnet , Bc Bonsager , B. Kramhoft , H. Mori , Ma Hachem et al.
Biologia, 2005, 60, pp.27-36
Article dans une revue hal-00314599 v1

Insights into the "pair of sugar tongs" surface binding site in barley alpha-amylase isozymes and crystallization of appropriate sugar tongs mutants

S. Tranier , K. Deville , X. Robert , Sophie Bozonnet , R. Haser et al.
Biologia, 2005, 60, pp.37-46
Article dans une revue hal-00314600 v1

Carbohydrate recognition by α-amylase isozymes: their crystal structures reveal new binding sites and remarkable functional differences

Richard Haser , Samuel Tranier , Xavier Robert , Birte Svensson , Nushin Aghajari
Acta Crystallographica Section A : Foundations of Crystallography [1968-2013], 2004, 60 (a1), pp.s166-s166. ⟨10.1107/S0108767304096710⟩
Article dans une revue hal-04914604 v1

The structure of barley alpha-amylase isozyme 1 reveals a novel role of domain C in substrate recognition and binding: a pair of sugar tongs.

X. Robert , R. Haser , Te Gottschalk , F. Ratajczak , H. Driguez et al.
Structure, 2003, 11, pp.973-984. ⟨10.1016/S0969-2126(03)00151-5⟩
Article dans une revue hal-00313539 v1

ESPript/ENDscript: Extracting and rendering sequence and 3D information from atomic structures of proteins.

P. Gouet , X. Robert , E. Courcelle
Nucleic Acids Research, 2003, 31, pp.3320-3323
Article dans une revue hal-00314281 v1

Comparison of crystal structures of barley alpha-amylase 1 and 2: implications for isozyme differences in stability and activity

X. Robert , R. Haser , B. Svensson , Xxxx Aghajarin
Biologia, 2002, 57, pp.59-70
Article dans une revue hal-00314580 v1

Expression, purification and preliminary crystallographic studies of alpha-amylase isozyme 1 from barley seeds.

X. Robert , Te Gottschalk , R. Haser , B. Svensson , N. Aghajari
Acta Crystallogr D Biol Crystallogr, 2002, 58, pp.683-686
Article dans une revue hal-00313546 v1