AB

ALHOSNA BENJDIA

Chargée de recherche INRAE, Université PARIS-SACLAY, Unité Micalis Co-cheffe de l'équipe ChemSyBio "Biochimie et Biologie synthétique"
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Présentation

10+ years experience in Biochemistry with a special focus on enzyme maturation, antibiotic, cofactor and natural product biosynthesis. Special emphasis on radical SAM enzymes within the microbiome. Experience in supervision of undergraduate and postgraduate students. Author of 40 publications including high impact journals.


Compétences

Biochemistry; Structural biology; Microbiome

Publications

Publications

Spectroscopic and Biochemical Characterization of the Noncanonical Radical SAM Enzyme ArsL, Involved in Arsinothricin Biosynthesis

Mickaël Mauger , Alain Guillot , Maryam Seif-Eddine , Stéphane Grimaldi , Alhosna Benjdia et al.
Journal of the American Chemical Society, 2025, 147 (24), pp.20381-20393. ⟨10.1021/jacs.5c01006⟩
Article dans une revue hal-05191495 v1

SARS-CoV-2 infectivity can be modulated through bacterial grooming of the glycocalyx

Cameron Martino , Benjamin Kellman , Daniel Sandoval , Thomas Mandel Clausen , Robert Cooper et al.
mBio, 2025, 16 (4), ⟨10.1128/mbio.04015-24⟩
Article dans une revue hal-05269525 v1
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Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme

Xavier Kubiak , Ivan Polsinelli , Leonard Chavas , Cameron Fyfe , Alain Guillot et al.
Nature Chemical Biology, In press, ⟨10.1038/s41589-023-01493-1⟩
Article dans une revue hal-04371487 v1

Radical S -Adenosyl- l -Methionine Enzyme PylB: A C-Centered Radical to Convert l -Lysine into (3 R )-3-Methyl- d -Ornithine

Feryel Soualmia , Mickael Cherrier , Timothée Chauviré , Mickaël Mauger , Philip Tatham et al.
Journal of the American Chemical Society, 2024, 146 (10), pp.6493-6505. ⟨10.1021/jacs.3c03747⟩
Article dans une revue hal-04496232 v1

B12-dependent radical SAM enzymes: Ever expanding structural and mechanistic diversity

Alhosna Benjdia , Olivier Berteau
Current Opinion in Structural Biology, 2023, 83, pp 102725. ⟨10.1016/j.sbi.2023.102725⟩
Article dans une revue hal-04343304 v1
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Exploring the biosynthetic capacity of TsrM, a B12‐dependent Radical SAM Enzyme Catalyzing Non‐radical Reactions

Feryel Soualmia , Alain Guillot , Nazarii Sabat , Clémence Brewee , Xavier Kubiak et al.
Chemistry - A European Journal, 2022, 28 (31), pp.e202200627. ⟨10.1002/chem.202200627⟩
Article dans une revue hal-03620811 v1
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Crystallographic snapshots of a B12-dependent radical SAM methyltransferase

Cameron Fyfe , Noelia Bernardo-García , Laura Fradale , Stéphane Grimaldi , Alain Guillot et al.
Nature, 2022, 602 (7896), pp.336-342. ⟨10.1038/s41586-021-04355-9⟩
Article dans une revue hal-03808863 v1
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The Epipeptide Biosynthesis Locus epeXEPAB Is Widely Distributed in Firmicutes and Triggers Intrinsic Cell Envelope Stress

Philipp F Popp , Lena Friebel , Alhosna Benjdia , Alain Guillot , Olivier Berteau et al.
Advances in Microbial Physiology, 2021, 31 (3), pp.1-12. ⟨10.1159/000516750⟩
Article dans une revue hal-03817729 v1

Radical SAM Enzymes and Ribosomally‐Synthesized and Post‐translationally Modified Peptides: A Growing Importance in the Microbiomes

Alhosna Benjdia , Olivier Berteau
Frontiers in Chemistry, 2021, 9, ⟨10.3389/fchem.2021.678068⟩
Article dans une revue hal-03808812 v1
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The Epipeptide YydF Intrinsically Triggers the Cell Envelope Stress Response of Bacillus subtilis and Causes Severe Membrane Perturbations

Philipp Popp , Alhosna Benjdia , Henrik Strahl , Olivier Berteau , Thorsten Mascher
Frontiers in Microbiology, 2020, 11, ⟨10.3389/fmicb.2020.00151⟩
Article dans une revue hal-02933776 v1
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Gold-Catalyzed Spirocyclization Reactions of N-Propargyl Tryptamines and Tryptophans in Aqueous Media

Nazarii Sabat , Feryel Soualmia , Pascal Retailleau , Alhosna Benjdia , Olivier Berteau et al.
Organic Letters, 2020, 22 (11), pp.4344-4349. ⟨10.1021/acs.orglett.0c01370⟩
Article dans une revue hal-03004181 v1
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Biosynthesis of the sactipeptide Ruminococcin C by the human microbiome: Mechanistic insights into thioether bond formation by radical SAM enzymes

Clémence Balty , Alain Guillot , Laura Fradale , Clémence Brewee , Benjamin Lefranc et al.
Journal of Biological Chemistry, 2020, 295 (49), pp.16665-16677. ⟨10.1074/jbc.RA120.015371⟩
Article dans une revue hal-03332307 v1

Gold-Catalyzed Spirocyclization Reactions of N -Propargyl Tryptamines and Tryptophans in Aqueous Media

Nazarii Sabat , Feryel Soualmia , Pascal Retailleau , Alhosna Benjdia , Olivier Berteau et al.
Organic Letters, 2020, 22 (14), pp.5714. ⟨10.1021/acs.orglett.0c02206⟩
Article dans une revue hal-02933787 v1
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Ruminococcin C, an anti-clostridial sactipeptide produced by a prominent member of the human microbiota Ruminococcus gnavus

Clémence Balty , Alain Guillot , Laura Fradale , Clémence Brewee , Mylène Boulay et al.
Journal of Biological Chemistry, 2019, 294 (40), pp.14512-14525. ⟨10.1074/jbc.RA119.009416⟩
Article dans une revue hal-02868868 v1
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Mechanistic Investigations of PoyD, a Radical S -Adenosyl- l -methionine Enzyme Catalyzing Iterative and Directional Epimerizations in Polytheonamide A Biosynthesis

Aubérie Parent , Alhosna A. Benjdia , Alain A. Guillot , Xavier Kubiak , Clémence Balty et al.
Journal of the American Chemical Society, 2018, 140 (7), pp.2469 - 2477. ⟨10.1021/jacs.7b08402⟩
Article dans une revue hal-01939282 v1

DNA repair by the radical SAM enzyme spore Photoproduct Lyase: from biochemistry to structural investigations

Olivier Berteau , Alhosna Benjdia
Photochemistry and Photobiology, 2017, 93 (1), pp.67-77. ⟨10.1111/php.12702⟩
Article dans une revue hal-01530836 v1
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Radical SAM enzymes in the biosynthesis of ribosomally synthesized and post-translationally modified peptides (RiPPs)

Alhosna Benjdia , Clémence Balty , Olivier Berteau
Frontiers in Chemistry, 2017, 5, ⟨10.3389/fchem.2017.00087⟩
Article dans une revue hal-02627786 v1
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Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis

Alhosna Benjdia , Alain Guillot , Pauline Ruffié , Jérôme Leprince , Olivier Berteau
Nature Chemistry, 2017, 9 (7), pp.698-707. ⟨10.1038/NCHEM.2714⟩
Article dans une revue hal-01960697 v1
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Insights into the catalysis of a lysine-tryptophan bond in bacterial peptides by a SPASM domain radical S-adenosylmethionine (SAM) peptide cyclase

Alhosna Benjdia , Laure L. Decamps , Alain Guillot , Xavier Kubiak , Pauline Ruffié et al.
Journal of Biological Chemistry, 2017, 292 (26), pp.10835-10844. ⟨10.1074/jbc.M117.783464⟩
Article dans une revue hal-02628401 v1

Sulfatases and radical SAM enzymes: emerging themes in glycosaminoglycan metabolism and the human microbiota

Alhosna Benjdia , Olivier Berteau
Biochemical Society Transactions, 2016, 44 (1), pp.109-115. ⟨10.1042/BST20150191⟩
Article dans une revue hal-01532684 v1

Carbon-sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE.

Roman Rohac , Patricia Amara , Alhosna Benjdia , Lydie Martin , Pauline Ruffié et al.
Nature Chemistry, 2016, 8 (5), pp.491-500. ⟨10.1038/NCHEM.2490⟩
Article dans une revue hal-01341083 v1
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The B-12-Radical SAM enzyme PoyC catalyzes valine C-beta-Methylation during Polytheonamide Biosynthesis

Auberie Parent , Alain Guillot , Alhosna Benjdia , Gwladys Chartier , Jerome Leprince et al.
Journal of the American Chemical Society, 2016, 138 (48), pp.15515-15518. ⟨10.1021/jacs.6b06697⟩
Article dans une revue hal-01605179 v1
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Thioether bond formation by SPASM domain radical SAM enzymes: C α H-atom abstraction in subtilosin A biosynthesis

Alhosna Benjdia , Alain Guillot , Benjamin Lefranc , Hubert Vaudry , Jérôme Leprince et al.
Chemical Communications, 2016, 52 (37), pp.6249-6252. ⟨10.1039/c6cc01317a⟩
Article dans une revue hal-01960692 v1
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The thiostrepton A tryptophan methyltransferase TsrM catalyses a cob(II)alamin-dependent methyl transfer reaction.

Alhosna Benjdia , Stéphane Pierre , Carmen Gherasim , Alain Guillot , Manon Carmona et al.
Nature Communications, 2015, 6, pp.8377. ⟨10.1038/ncomms9377⟩
Article dans une revue hal-01216185 v1

Rescuing DNA repair activity by rewiring the H-atom transfer pathway in the radical SAM enzyme, spore photoproduct lyase

Alhosna A. Benjdia , Korbinian Heil , Andreas Winkler , Thomas Carell , Ilme Schlichting
Chemical Communications, 2014, 50 (91), pp.14201-14204. ⟨10.1039/C4CC05158K⟩
Article dans une revue hal-02631377 v1
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Characterization of Gglycosaminoglycan (GAG) sulfatases from the human gut symbiont bacteroides thetaiotaomicron reveals the first GAG-specific bacterial endosulfatase

Jonathan Ulmer , Eric Morssing Vilen , Ramesh Babu Namburi , Alhosna Benjdia , Julie Beneteau et al.
Journal of Biological Chemistry, 2014, 289 (35), pp.24289 - 24303. ⟨10.1074/jbc.M114.573303⟩
Article dans une revue hal-01204451 v1

A radical transfer pathway in spore photoproduct lyase

Linlin Yang , Renae S. Nelson , Alhosna A. Benjdia , Gengjie Lin , Joshua Telser et al.
Biochemistry, 2013, 52 (18), pp.3041-3050. ⟨10.1021/bi3016247⟩
Article dans une revue hal-02642679 v1

Chondroitin-4-O-sulfatase from Bacteroides thetaiotaomicron: exploration of the substrate specificity

Annie Malleron , Alhosna Benjdia , Olivier Berteau , Christine Le Narvor
Carbohydrate Research, 2012, 353, pp.96 - 99. ⟨10.1016/j.carres.2012.03.033⟩
Article dans une revue istex hal-01003430 v1
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Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme

Alhosna A. Benjdia , Korbinian Heil , Thomas R. M. Barends , Thomas R. M. Carell , Ilme Schlichting
Nucleic Acids Research, 2012, 40 (18), pp.9308-9318. ⟨10.1093/nar/gks603⟩
Article dans une revue hal-02647062 v1

Thiostrepton tryptophan methyltransferase expands the chemistry of radical SAM enzymes

Stéphane S. Pierre , Alain A. Guillot , Alhosna A. Benjdia , Corine C. Sandstrom , Philippe P. Langella et al.
Nature Chemical Biology, 2012, 8 (12), pp.957 - 959. ⟨10.1038/NCHEMBIO.1091⟩
Article dans une revue hal-01004114 v1

DNA photolyases and SP lyase: structure and mechanism of light-dependent and independent DNA lyases

Alhosna A. Benjdia
Current Opinion in Structural Biology, 2012, 22 (6), pp.711-720. ⟨10.1016/j.sbi.2012.10.002⟩
Article dans une revue istex hal-02651842 v1

Biosynthesis of F-0, Precursor of the F-420 Cofactor, Requires a Unique Two Radical-SAM Domain Enzyme and Tyrosine as Substrate

Laure L. Decamps , Benjamin B. Philmus , Alhosna A. Benjdia , Robert R. White , Tadhg P. T. P. Begley et al.
Journal of the American Chemical Society, 2012, 134 (44), pp.18173 - 18176. ⟨10.1021/ja307762b⟩
Article dans une revue hal-01004118 v1
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Sulfatases and a Radical S-Adenosyl-L-methionine (AdoMet) Enzyme Are Key for Mucosal Foraging and Fitness of the Prominent Human Gut Symbiont, Bacteroides thetaiotaomicron

Alhosna A. Benjdia , Eric C. E. C. Martens , Jeffrey I. J. I. Gordon , Olivier Berteau
Journal of Biological Chemistry, 2011, 286 (29), pp.25973 - 25982. ⟨10.1074/jbc.M111.228841⟩
Article dans une revue hal-01004563 v1

Anaerobic sulfatase-maturating enzyme - A mechanistic link with glycyl radical-activating enzymes?

Alhosna Benjdia , Sowmya Subramanian , Jérôme Leprince , Hubert Vaudry , Michael Johnson et al.
FEBS Journal, 2010, 277 (8), pp.1906-1920. ⟨10.1111/j.1742-4658.2010.07613.x⟩
Article dans une revue hal-01960689 v1

An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily

Bert van Loo , Stefanie Jonas , Ann C. Babtie , Alhosna A. Benjdia , Olivier Berteau et al.
Proceedings of the National Academy of Sciences of the United States of America, 2010, 107 (7), pp.2740-2745. ⟨10.1073/pnas.0903951107⟩
Article dans une revue hal-02663877 v1

Mechanistic Investigations of Anaerobic Sulfatase-Maturating Enzyme: Direct C β H-Atom Abstraction Catalyzed by a Radical AdoMet Enzyme

Alhosna Benjdia , Corine C. Sandstrom , Hubert Vaudry , Olivier Berteau , Jérôme Leprince et al.
Journal of the American Chemical Society, 2009, 131 (24), pp.8348-8349. ⟨10.1021/ja901571p⟩
Article dans une revue hal-01960686 v1
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Anaerobic Sulfatase-maturating Enzymes, First Dual Substrate Radical S -Adenosylmethionine Enzymes

Alhosna Benjdia , Sowmya Subramanian , Jérôme Leprince , Hubert Vaudry , Michael Johnson et al.
Journal of Biological Chemistry, 2008, 283 (26), pp.17815-17826. ⟨10.1074/jbc.M710074200⟩
Article dans une revue hal-01960684 v1

Anaerobic Sulfatase-Maturating Enzymes: Radical SAM Enzymes Able To Catalyze in Vitro Sulfatase Post-translational Modification

Alhosna Benjdia , Jérôme Leprince , Alain Guillot , Hubert Vaudry , Sylvie Rabot et al.
Journal of the American Chemical Society, 2007, 129 (12), pp.3462-3463. ⟨10.1021/ja067175e⟩
Article dans une revue istex hal-01960680 v1

First evidences for a third sulfatase maturation system in prokaryotes from E. coli aslB and ydeM deletion mutants

Alhosna Benjdia , Gianni Dehò , Sylvie Rabot , Olivier Berteau
FEBS Letters, 2007, 581 (5), pp.1009-1014. ⟨10.1016/j.febslet.2007.01.076⟩
Article dans une revue istex hal-02659709 v1
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A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes

Olivier Berteau , Alain A. Guillot , Alhosna Benjdia , Sylvie S. Rabot
Journal of Biological Chemistry, 2006, 281 (32), pp.22464-22470. ⟨10.1074/jbc.M602504200⟩
Article dans une revue hal-02656640 v1

Radically new methylation reactions in antibiotic biosynthesis: Insights into the mechanism of B12-dependent radical SAM enzymes

Olivier Berteau , Stéphane Pierre , Alhosna Benjdia , Carmen Gherasim , Ruma Banerjee
Experimental Biology Meeting, Mar 2015, Boston, United States
Communication dans un congrès hal-01536523 v1

Structural perspectives on the mechanism of the radical SAM enzyme, spore photoproduct lyase

Alhosna Benjdia , Korbinian Heil , Andreas Winkler , Thomas Carell , Ilme Schlichting
Experimental Biology Meeting, Mar 2015, Boston, United States
Communication dans un congrès hal-01536522 v1

Spore Photoproduct Lyase, a radically different DNA repair enzyme for bacterial spore extreme UV-resistance

Alhosna Benjdia , Thomas Barends , Ilme Schlichting , Korbinian Heil , Thomas Carell
16. International Conference on Biological Inorganic Chemistry (ICBIC), IMBG. FRA., Jul 2013, Grenoble, France. 1 p
Communication dans un congrès hal-01204366 v1

One enzyme for two radical reactions: Deciphering the mechanism of a novel radical SAM enzyme critical for F-420 cofactor biosynthesis

Laure Decamps , Alhosna Benjdia , Olivier Berteau , Benjamin Philmus , Tadhg P. Begley et al.
16. International Conference on Biological Inorganic Chemistry (ICBIC), IMBG. FRA., Jul 2013, Grenoble, France. 1 p
Communication dans un congrès hal-01204368 v1

Investigation of a critical radical SAM enzyme involved in co-factor biosynthesis in Actinobacteria

Laure Decamps , Alhosna Benjdia , R. H. White , Olivier Berteau
22. IUBMB Congress, Sep 2012, Seville, Spain. 608 p
Communication dans un congrès hal-01189734 v1

Novel methyl transfer reactions: expanding the chemistry of radical SAM enzymes

Stéphane Pierre , Alain Guillot , Alhosna Benjdia , Philippe Langella , Olivier Berteau
22. IUBMB Congress, Sep 2012, Seville, Spain. 608 p
Communication dans un congrès hal-01189735 v1

Insight into a new type of methyltransferase belonging to the radical AdoMet enzyme superfamily: the antibiotic resistance enzyme Cfr from [i]Staphylococcus aureus[/i]

Gaëlle Vassiliadis , Alhosna Benjdia , C. Sandström , V. Guerineau , Olivier Berteau
22. IUBMB Congress, Sep 2012, Seville, Spain. 608 p
Communication dans un congrès hal-01189736 v1

B12-Dependant Radical SAM Enzymes: An Emerging Family of Biocatalysts Catalyzing Peptide and Protein Post-Anslational Modifiations

Thibaut Bourdin , Alain Guillot , Stéphane Grimaldi , Benjamin Lefranc , Cameron Fyfe et al.
47th FEBS Congress, Jul 2023, Tours, France. 2023
Poster de conférence hal-05168057 v1

A radical alternative for methyl transfer reactions: Emergence of the B12-binding/radical SAM-domain enzymes

Olivier Berteau , S. Pierre , Alain Guillot , Alhosna Benjdia , Philippe Langella et al.
16. International Conference on Biological Inorganic Chemistry (ICBIC), Jul 2013, Grenoble, France. Springer, JBIC. Journal of Biological Inorganic Chemistry, 19, 1 p., 2014, XVIth International Conference on Biological Inorganic Chemistry
Poster de conférence hal-01204367 v1