PC

Patrick Chames

63
Documents
Affiliations actuelles
  • Centre National de la Recherche Scientifique (CNRS)
  • Institut National de la Santé et de la Recherche Médicale (INSERM)
  • Centre de Recherche en Cancérologie de Marseille (CRCM)
  • Institut Paoli-Calmettes (IPC)
  • Aix Marseille Université (AMU)
Identifiants chercheurs
Contact

Présentation

The main interest of our lab is to use antibody engineering solutions to generate innovative molecules able to efficiently modulate the anti-tumor immune response through the recruitment and activation of immune cells using multispecific cell engagers or multispecific immune checkpoint inhibitors, or through the vectorization of radionuclides and nanoparticles.

Our team has developed a solid expertise in the generation and use of nanobodies, corresponding to the variable domain of antibodies naturally devoid of light chain found in camelids, for cancer therapy and diagnostic using in vitro selection methods. We are exploring the possibilities offered by this technology for the development of innovative cancer imaging and therapy approaches.

Domaines de recherche

Cancer Immunothérapie

Publications

Publications

Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier

Marion David , Romy Cohen , Diane Beuzelin , Géraldine Ferracci , Marina Cresci et al.
Article dans une revue hal-05088982 v1

Epitope Identification of an mGlu5 Receptor Nanobody Using Physics-Based Molecular Modeling and Deep Learning Techniques

Floriane Eshak , Léo Pion , Pauline Scholler , Damien Nevoltris , Patrick Chames et al.
Journal of Chemical Information and Modeling, 2024, 64 (11), pp.4436-4461. ⟨10.1021/acs.jcim.3c01620⟩
Article dans une revue hal-04723363 v1
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Antigen density and applied force control enrichment of nanobody-expressing yeast cells in microfluidics

Merlin Sanicas , Remy Torro , Laurent Limozin , Patrick Chames
Lab on a Chip, 2024, Online ahead of print. ⟨10.1039/D4LC00011K⟩
Article dans une revue hal-04562911 v2
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Specific Targeting of Mesothelin-Expressing Malignant Cells Using Nanobody-Functionalized Magneto-Fluorescent Nanoassemblies

Tina Briolay , Judith Fresquet , Damien Meyer , Brigitte Kerfelec , Patrick Chames et al.
International Journal of Nanomedicine, 2024, 19, pp.633 - 650. ⟨10.2147/ijn.s435787⟩
Article dans une revue inserm-04465814 v1
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Combination of T cell-redirecting strategies with a bispecific antibody blocking TGF-β and PD-L1 enhances antitumor responses

Antonio Tapia-Galisteo , Iñigo Sánchez-Rodríguez , Javier Narbona , Patricia Iglesias-Hernández , Saray Aragón-García et al.
OncoImmunology, 2024, 13 (1), pp.2338558. ⟨10.1080/2162402X.2024.2338558⟩
Article dans une revue hal-04583762 v1
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A single-domain antibody for the detection of pathological Tau protein in the early stages of oligomerization

Nicolas de Leiris , Pascale Perret , Charlotte Lombardi , Bülent Gözel , Sabine Chierici et al.
Journal of Translational Medicine, 2024, 22 (1), pp.163. ⟨10.1186/s12967-024-04987-1⟩
Article dans une revue hal-04583802 v1
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Nanobody-based sensors reveal a high proportion of mGlu heterodimers in the brain

Jiyong Meng , Chanjuan Xu , Pierre-André Lafon , Salomé Roux , Michaël Mathieu et al.
Nature Chemical Biology, 2022, 18, pp.894-903. ⟨10.1038/s41589-022-01050-2⟩
Article dans une revue hal-03699580 v1
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Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer

Antonio Tapia-Galisteo , Íñigo Sánchez Rodríguez , Oscar Aguilar-Sopeña , Seandean Lykke Harwood , Javier Narbona et al.
OncoImmunology, 2022, 11, ⟨10.1080/2162402x.2022.2034355⟩
Article dans une revue hal-03745498 v1
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A nanobody activating metabotropic glutamate receptor 4 discriminates between homo- and heterodimers

Jordi Haubrich , Joan Font , Robert Quast , Anne Goupil-Lamy , Pauline Scholler et al.
Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (33), pp.e2105848118. ⟨10.1073/pnas.2105848118⟩
Article dans une revue hal-03322348 v1
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Anti-NKG2D single domain-based antibodies for the modulation of anti-tumor immune response

Adeline Raynaud , Klervi Desrumeaux , Laurent Vidard , Elise Termine , Daniel Baty et al.
OncoImmunology, 2020, 10, ⟨10.1080/2162402x.2020.1854529⟩
Article dans une revue hal-03371613 v1
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Special Issue: Nanobody

Patrick Chames , Ulrich Rothbauer
Antibodies, 2020, 9 (1), pp.6. ⟨10.3390/antib9010006⟩
Article dans une revue hal-03017191 v1
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A Novel Anti-Kv10.1 Nanobody Fused to Single-Chain TRAIL Enhances Apoptosis Induction in Cancer Cells

Franziska Hartung , Thomas Krüwel , Xiaoyi Shi , Klaus Pfizenmaier , Roland Kontermann et al.
Frontiers in Pharmacology, 2020, 11, ⟨10.3389/fphar.2020.00686⟩
Article dans une revue hal-03017218 v1
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Nanobody Engineering: Toward Next Generation Immunotherapies and Immunoimaging of Cancer

Timothée Chanier , Patrick Chames
Antibodies, 2019, 8 (1), pp.13. ⟨10.3390/antib8010013⟩
Article dans une revue hal-02002577 v1
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Nanobody-CD16 Catch Bond Reveals NK Cell Mechanosensitivity

Cristina González , Patrick Chames , Brigitte Kerfelec , Daniel Baty , Philippe Robert et al.
Biophysical Journal, 2019, 116 (8), pp.1516-1526. ⟨10.1016/j.bpj.2019.03.012⟩
Article dans une revue hal-02323625 v1
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A Bispecific Antibody-Based Approach for Targeting Mesothelin in Triple Negative Breast Cancer

Joanie del Bano , Rémy Florès-Florès , Emmanuelle Josselin , Armelle Goubard , Laëtitia Ganier et al.
Frontiers in Immunology, 2019, 10, pp.1593. ⟨10.3389/fimmu.2019.01593⟩
Article dans une revue hal-02520930 v1
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Les anticorps et scaffold bispécifiques, des médicaments innovants en oncologie impliquant le ciblage des cellules immunitaires

Patrick Chames , Thierry Wurch
Médecine/Sciences, 2019, 35 (12), pp.1072-1082. ⟨10.1051/medsci/2019242⟩
Article dans une revue hal-03017227 v1

Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays

Alyona Sukhanova , Fernanda Ramos-Gomes , Patrick Chames , Daniel Baty , Frauke Alves et al.
Proceedings of SPIE, the International Society for Optical Engineering, 2019, pp.112072A. ⟨10.1117/12.2530233⟩
Article dans une revue hal-03007502 v1

Advanced Nanotools for Imaging of Solid Tumors and Circulating and Disseminated Cancer Cells

D. Baty , I. Nabiev , A. Sukhanova , F. Ramos-Gomes , F. Alves et al.
Optics and Spectroscopy, 2018, 125 (5), pp.703-707. ⟨10.1134/S0030400X18110309⟩
Article dans une revue hal-02143643 v1
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Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots

Fernanda Ramos-Gomes , Julia Bode , Alyona Sukhanova , Svetlana V Bozrova , Mara Saccomano et al.
Scientific Reports, 2018, 8 (1), pp.4595. ⟨10.1038/s41598-018-22973-8⟩
Article dans une revue hal-01765351 v1
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Allosteric nanobodies uncover a role of hippocampal mGlu2 receptor homodimers in contextual fear consolidation

Pauline Scholler , Damien Nevoltris , Dimitri de Bundel , Simon Bossi , David Moreno-Delgado et al.
Nature Communications, 2017, 8 (1), pp.1967. ⟨10.1038/s41467-017-01489-1⟩
Article dans une revue hal-01672805 v2

Nanosized Fluorescent Diagnostic Probes Consisting of Single-domain Antibodies Conjugated with Quantum Dots

Alyona Sukhanova , Klervi Even-Desrumeaux , Jean-Marc Millot , Patrick Chames , Daniel Baty et al.
Materials Today: Proceedings, 2016, 3 (2), pp.518-522. ⟨10.1016/j.matpr.2016.01.083⟩
Article dans une revue hal-03112307 v1

Multiphoton Imaging of Tumor Biomarkers in situ Using Single-domain Antibodies Conjugated with Quantum Dots in a Set Orientation

Alyona Sukhanova , Hilal Hafian , Marc Turini , Patrick Chames , Daniel Baty et al.
Materials Today: Proceedings, 2016, 3 (2), pp.523-526. ⟨10.1016/j.matpr.2016.01.084⟩
Article dans une revue hal-03112311 v1
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V1/V2 Neutralizing Epitope is Conserved in Divergent Non-M Groups of HIV-1

Marion Morgand , Mélanie Bouvin-Pley , Jean-Christophe Plantier , Moreau Alain , Elodie Alessandri et al.
Journal of Acquired Immune Deficiency Syndromes - JAIDS, 2016, 71 (3), pp.237-245. ⟨10.1097/QAI.0000000000000854⟩
Article dans une revue hal-01314296 v1

In vivo detection of small tumour lesions by multi-pinhole SPECT applying a (99m)Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor.

Thomas Krüwel , Damien Nevoltris , Julia Bode , Christian Dullin , Daniel Baty et al.
Scientific Reports, 2016, 6, pp.21834
Article dans une revue hal-01450793 v1
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Taking up Cancer Immunotherapy Challenges: Bispecific Antibodies, the Path Forward?

Joanie del Bano , Patrick Chames , Daniel Baty , Brigitte Kerfelec
Antibodies, 2016, 5 (1), ⟨10.3390/antib5010001⟩
Article dans une revue hal-01450799 v1

Conformational nanobodies reveal tethered epidermal growth factor receptor involved in EGFR/ErbB2 predimers.

Damien Nevoltris , Benjamin Lombard , Elodie Dupuis , Gérard Mathis , Patrick Chames et al.
ACS Nano, 2015, 9 (2), pp.1388-99. ⟨10.1021/nn505752u⟩
Article dans une revue hal-01450765 v1
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Oriented Conjugates of Single-domain Antibodies and Fluorescent Quantum Dots for Highly Sensitive Detection of Tumor-associated Biomarkers in Cells and Tissues

Regina Bilan , Kristina Brazhnik , Patrick Chames , Daniel Baty , Igor Nabiev et al.
Physics Procedia, 2015, 73, pp.228-234. ⟨10.1016/j.phpro.2015.09.162⟩
Article dans une revue hal-03112376 v1

Detection of carcinoembryonic antigen using single-domain or full-size antibodies stained with quantum dot conjugates.

Gilles Rousserie , Regina Grinevich , Kristina Brazhnik , Klervi Even-Desrumeaux , Brigitte Reveil et al.
Analytical Biochemistry, 2015, 478, pp.26-32. ⟨10.1016/j.ab.2015.02.029⟩
Article dans une revue hal-01450782 v1

Anti-Mesothelin Nanobodies for Both Conventional and Nanoparticle-Based Biomedical Applications.

Andrew M Prantner , Marc Turini , Brigitte Kerfelec , Shree Joshi , Daniel Baty et al.
Journal of Biomedical Nanotechnology, 2015, pp.1201-12
Article dans une revue hal-01450786 v1

Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells

Tatsiana Rakovich , Omar Mahfoud , Bashir Mohamed , Adriele Prina-Mello , Kieran Crosbie-Staunton et al.
ACS Nano, 2014, 8 (6), pp.5682-5695. ⟨10.1021/nn500212h⟩
Article dans une revue hal-03136167 v1
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A FcγRIII-engaging bispecific antibody expands the range of HER2-expressing breast tumors eligible to antibody therapy

Marc Turini , Patrick Chames , Pierre Bruhns , Daniel Baty , Brigitte Kerfelec
Oncotarget, 2014, 5 (14), ⟨10.18632/oncotarget.2093⟩
Article dans une revue hal-02115462 v1
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Selection of Intracellular Single-Domain Antibodies Targeting the HIV-1 Vpr Protein by Cytoplasmic Yeast Two-Hybrid System

Julie Matz , Cécile Hérate , Jérôme Bouchet , Nelson Dusetti , Odile Gayet et al.
PLoS ONE, 2014, 9 (12), pp.e113729. ⟨10.1371/journal.pone.0113729⟩
Article dans une revue inserm-01093997 v1

Multiphoton imaging of tumor biomarkers with conjugates of single-domain antibodies and quantum dots

Hilal Hafian , Alyona Sukhanova , Marc Turini , Patrick Chames , Daniel Baty et al.
Nanomedicine: Nanotechnology, Biology and Medicine, 2014, 10 (8), pp.1701-1709. ⟨10.1016/j.nano.2014.05.014⟩
Article dans une revue hal-03136281 v1
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Masked Selection: A Straightforward and Flexible Approach for the Selection of Binders Against Specific Epitopes and Differentially Expressed Proteins by Phage Display

Klervi Even-Desrumeaux , Damien Nevoltris , Marie Noelle Lavaut , Karima Alim , Jean-Paul Borg et al.
Molecular and Cellular Proteomics, 2014, 13 (2), pp.653-665. ⟨10.1074/mcp.O112.025486⟩
Article dans une revue hal-02115444 v1

Single-Domain Antibody-Based and Linker-Free Bispecific Antibodies Targeting Fc RIII Induce Potent Antitumor Activity without Recruiting Regulatory T Cells

C. Rozan , A. Cornillon , C. Petiard , M. Chartier , G. Behar et al.
Molecular Cancer Therapeutics, 2013, 12 (8), pp.1481-1491. ⟨10.1158/1535-7163.MCT-12-1012⟩
Article dans une revue hal-02115468 v1

Heavy Chain-Only IgG2b Llama Antibody Effects Near-Pan HIV-1 Neutralization by Recognizing a CD4-Induced Epitope That Includes Elements of Coreceptor- and CD4-Binding Sites

P. Acharya , T. Luongo , I. Georgiev , J. Matz , S. Schmidt et al.
Journal of Virology, 2013, 87 (18), pp.10173-10181. ⟨10.1128/JVI.01332-13⟩
Article dans une revue hal-02530765 v1
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Single-domain antibody-based and linker-free bispecific antibodies targeting FcγRIII induce potent antitumor activity without recruiting regulatory T cells.

Caroline Rozan , Amélie Cornillon , Corinne Petiard , M. Chartier , G. Behar et al.
Molecular Cancer Therapeutics, 2013, 12 (8), pp.1481-1491. ⟨10.1158/1535-7163.MCT-12-1012⟩
Article dans une revue hal-02454959 v1
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Straightforward selection of broadly neutralizing single-domain antibodies targeting the conserved CD4 and co-receptor binding sites of HIV-1 gp120

Julie Matz , Pacal Kessler , Jérome Bouchet , Olivier Combes , Daniel Baty et al.
Retrovirology, 2012, 9 (Suppl 2), pp.P214
Article dans une revue inserm-00731772 v1

Oriented conjugates of monoclonal and single-domain antibodies with quantum dots for flow cytometry and immunohistochemistry diagnostic applications

Alyona Sukhanova , Klervi Even-Desrumeaux , Jean-Marc Millot , Patrick Chames , Daniel Baty et al.
Proceedings of SPIE, the International Society for Optical Engineering, 2012, Colloidal Nanocrystals for Biomedical Applications VII, 8232, pp.82320T. ⟨10.1117/12.905896⟩
Article dans une revue hal-03184792 v1

Oriented conjugates of single-domain antibodies and quantum dots: toward a new generation of ultrasmall diagnostic nanoprobes

Alyona Sukhanova , Klervi Even-Desrumeaux , Aymric Kisserli , Thierry Tabary , Brigitte Reveil et al.
Nanomedicine: Nanotechnology, Biology and Medicine, 2012, 8 (4), pp.516-525. ⟨10.1016/j.nano.2011.07.007⟩
Article dans une revue istex hal-03184846 v1
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Structural definition of a novel CD4-induced epitope that is targeted by a single-headed immunoglobulin to effect broad and potent HIV neutralization

Priyamvada Acharya , Timothy S. Luongo , Julie Matz , Stephen D. Schmidt , Gwo-Yu Chuang et al.
Retrovirology, 2012, 9 (Suppl 2), pp.P346
Article dans une revue inserm-00731776 v1
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Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches.

Klervi Even-Desrumeaux , Patrick Fourquet , Véronique Secq , Daniel Baty , Patrick Chames
Molecular BioSystems, 2012, 8 (9), pp.2385-2394. ⟨10.1039/c2mb25063b⟩
Article dans une revue inserm-00722930 v1

Bi-photon imaging and diagnostics using ultra-small diagnostic probes engineered from semiconductor nanocrystals and single-domain antibodies

Hilal Hafian , Alyona Sukhanova , Patrick Chames , Daniel Baty , Michel Pluot et al.
Proceedings of SPIE, the International Society for Optical Engineering, 2012, Nanosystems in Engineering and Medicine, 8548, pp.85480R. ⟨10.1117/12.960493⟩
Article dans une revue hal-03177842 v1

Straightforward Selection of Broadly Neutralizing Single-Domain Antibodies Targeting the Conserved CD4 and Coreceptor Binding Sites of HIV-1 gp120

J. Matz , P. Kessler , J. Bouchet , O. Combes , O. Ramos et al.
Journal of Virology, 2012, 87 (2), pp.1137-1149. ⟨10.1128/JVI.00461-12⟩
Article dans une revue cea-02530610 v1
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Strong and oriented immobilization of single domain antibodies from crude bacterial lysates for high-throughput compatible cost-effective antibody array generation.

Klervi Even-Desrumeaux , Daniel Baty , Patrick Chames
Molecular BioSystems, 2010, 6 (11), pp.2241-8. ⟨10.1039/c005279e⟩
Article dans une revue inserm-00521371 v1
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Therapeutic antibodies for the treatment of pancreatic cancer.

Patrick Chames , Brigitte Kerfelec , Daniel Baty
TheScientificWorldJournal, 2010, 10, pp.1107-20. ⟨10.1100/tsw.2010.103⟩
Article dans une revue inserm-00497886 v1
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Therapeutic Antibodies for the Treatment of Pancreatic Cancer

Patrick Chames , Brigitte Kerfelec , Daniel Baty
The Scientific World Journal, 2010, 10, pp.1107-1120. ⟨10.1100/tsw.2010.103⟩
Article dans une revue hal-02115485 v1

Semiconductor quantum dots for multiplexed bio-detection on solid-state microarrays.

G. Rousserie , A. Sukhanova , K. Even-Desrumeaux , F. Fleury , P. Chames et al.
Critical Reviews in Oncology/Hematology, 2009, 00 (00), pp.00
Article dans une revue hal-00415311 v1

Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen.

G. Behar , P. Chames , I. Teulon , A. Cornillon , F. Alshoukr et al.
FEBS Journal, 2009, june (00), pp.00
Article dans une revue hal-00414287 v1

Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen.

Ghislaine Behar , Patrick Chames , Isabelle Teulon , Amélie Cornillon , Faisal Alshoukr et al.
FEBS Journal, 2009, 276 (14), pp.3881-93. ⟨10.1111/j.1742-4658.2009.07101.x⟩
Article dans une revue hal-00420547 v1
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Bispecific antibodies for cancer therapy: the light at the end of the tunnel?

Patrick Chames , Daniel Baty
mAbs, 2009, 1 (6), pp.539-47
Article dans une revue inserm-00448211 v1
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[The future of antibody fragments, made of a single immunoglobulin domain.]

Patrick Chames , Daniel Baty
Médecine/Sciences, 2009, 25 (12), pp.1159-62
Article dans une revue inserm-00443810 v1

Isolation and characterization of anti-Fc{gamma}RIII (CD16) llama single-domain antibodies that activate natural killer cells.

Ghislaine Behar , Sophie Sibéril , Agnès Groulet , Patrick Chames , Martine Pugnière et al.
Protein Engineering, Design and Selection, 2008, 21 (1), pp.1-10. ⟨10.1093/protein/gzm064⟩
Article dans une revue hal-00258940 v1
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Isolation and characterization of anti-FcgRIII (CD16) llama single-domain antibodies that activate natural killer cells

Ghislaine Béhar , Sophie Sibéril , Agnès Groulet , Patrick Chames , Martine Pugnière et al.
Protein Engineering, Design and Selection, 2007, 21 (1), pp.1-10. ⟨10.1093/protein/gzm064⟩
Article dans une revue hal-02454965 v1

Intracellular Immunization of Prokaryotic Cells against a Bacteriotoxin

Patrick Chames , Jacques Fieschi , Daniel Baty , Denis Duché
Journal of Bacteriology, 1998, 180 (3), pp.514-518. ⟨10.1128/JB.180.3.514-518.1998⟩
Article dans une revue hal-03269515 v1